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Dear Zdenek,
our super-res research on the effects of ischemic (hypoxic) environment on
chromatin architecture might be of your interest:
https://genomebiology.biomedcentral.com/articles/10.1186/s13059-015-0802-2(here we used a hypoxia chamber to decrease oxygen concentration in culture
atmosphere)
best regards
Aleks
2018-01-29 17:43 GMT+01:00 <
[hidden email]>:
> *****
> To join, leave or search the confocal microscopy listserv, go to:
>
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy> Post images on
http://www.imgur.com and include the link in your posting.
> *****
>
> Dear Listers,
> when going through the product sheets of Prolong Live (an anti-
> photobleaching agent for live-cell fluorescence imaging) and OxyFluor (an
> oxygen-consuming membrane fraction of E. coli), I couldn't help but notice
> some similarity...
>
>
https://assets.thermofisher.com/TFS-Assets/LSG/manuals/> ProLong_Live_Antifade
> _Reagent_UG.pdf
>
>
>
https://www.oxyrase.com/upload/product_inserts/lab.
> 0093.v006_-_oxyfluor_pi.
> pdf
> Can someone confirm that Prolong Live is indeed based on OxyFluor?
>
> Which brings up the question: How happy are the cells gonna be in an
> oxygen-
> scavenging environment?
>
> Has anyone tried combining this with refractive index matching, such as
> iodixanol (OptiPrep) or Histodenz or other?
>
> Thanks for any tips or comments!
>
> No commercial interest.
>
>
> Best, zdenek
>
> --
> Zdenek Svindrych, Ph.D.
> Research Associate - Imaging Specialist
> Department of Biochemistry and Cell Biology
> Geisel School of Medicine at Dartmouth
> email:
[hidden email]
>
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