A while ago I noted that long shift dyes like the Atto-LS are disappearing from the American market. Some folks responded with dyes used in flow cytometry (thanks again George!), but I couldn't find any that are currently marketed for fluorescence imaging.
Before I start investing in trial and error, is anyone currently using long-shift dyes for fluorescence multiplex imaging? If you do, I'd gratefully appreciate any advice you have re their use.
Thanks and all the best,
Timothy Feinstein, Ph.D.
Research Scientist, University of Pittsburgh Department of Developmental Biology