Looking for the coverslips unicorn

[lechristophe]

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Hi,

We are looking for 18 mm round coverslips #1.5H (170 ± 5 µm), gridded
(photo-etched) for correlative microscopy. Would you know anything
approaching (could do with other formats), or a good place to ask to have
them custom made?

Thank you for your help,

Christophe

--
Christophe Leterrier
NeuroCyto lab
NICN CNRS UMR 7259
Aix Marseille University, France

[Rusty Nicovich]

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We've done similar things in the fab facility on campus. Not sure how
special that equipment is - it was part of the "National Fabrication
Facility" in Australia - but any place with a decent electrical engineering
program would likely have at least one piece of gear that could make
photo-etched coverslips.

You could do it in your own lab if you're feeling industrious.  Coat glass
in negative photoresist, draw some lines with the user-defined FRAP
settings on the scope @ 405 nm, then etch a bit. Clean off resist in
acetone and you should be set.  I say 'should' because I have never
actually done this, but the YouTube videos I've seen make it look easy.

Rusty

On Thu, Oct 26, 2017 at 2:53 AM, Christophe Leterrier <
[hidden email]> wrote:

> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> Post images on http://www.imgur.com and include the link in your posting.
> *****
>
> Hi,
>
> We are looking for 18 mm round coverslips #1.5H (170 ± 5 µm), gridded
> (photo-etched) for correlative microscopy. Would you know anything
> approaching (could do with other formats), or a good place to ask to have
> them custom made?
>
> Thank you for your help,
>
> Christophe
>
> --
> Christophe Leterrier
> NeuroCyto lab
> NICN CNRS UMR 7259
> Aix Marseille University, France
>

[preynolds]

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Great point Rusty - I didn't think of that and I'm supposed to be the fabrication guy!

We have done photolithography on our Zeiss LSM800 in the manner you describe. This worked for features down to tens of microns. We used a microposit resist ( http://microchem.com/products/images/uploads/S1800_Photoresist.pdf ) and I can help with a protocol if anyone is interested.

Believe in unicorns.
Cheers,
Paul

--
Paul Reynolds, Biomedical Engineering
University of Glasgow

[hidden email]
[phone]

Google Scholar: https://goo.gl/0yRpwg
ResearchGate:  http://goo.gl/9lyQq1


________________________________________
From: Confocal Microscopy List [[hidden email]] on behalf of Rusty Nicovich [[hidden email]]
Sent: 26 October 2017 17:49
To: [hidden email]
Subject: Re: Looking for the coverslips unicorn

*****
To join, leave or search the confocal microscopy listserv, go to:
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*****

We've done similar things in the fab facility on campus. Not sure how
special that equipment is - it was part of the "National Fabrication
Facility" in Australia - but any place with a decent electrical engineering
program would likely have at least one piece of gear that could make
photo-etched coverslips.

You could do it in your own lab if you're feeling industrious.  Coat glass
in negative photoresist, draw some lines with the user-defined FRAP
settings on the scope @ 405 nm, then etch a bit. Clean off resist in
acetone and you should be set.  I say 'should' because I have never
actually done this, but the YouTube videos I've seen make it look easy.

Rusty

On Thu, Oct 26, 2017 at 2:53 AM, Christophe Leterrier <
[hidden email]> wrote:

> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> Post images on http://www.imgur.com and include the link in your posting.
> *****
>
> Hi,
>
> We are looking for 18 mm round coverslips #1.5H (170 ± 5 µm), gridded
> (photo-etched) for correlative microscopy. Would you know anything
> approaching (could do with other formats), or a good place to ask to have
> them custom made?
>
> Thank you for your help,
>
> Christophe
>
> --
> Christophe Leterrier
> NeuroCyto lab
> NICN CNRS UMR 7259
> Aix Marseille University, France
>

[DamirSudar]

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Hi Christophe,

Probably not exactly what you need but have you looked at Ibidi's
mini-chamber slide/coverslips?
See: https://ibidi.com/channel-slides/61--slide-corrsight-tm-live.html

I have no connection with them except being a customer.

Cheers,
- Damir

> On Thu, Oct 26, 2017 at 2:53 AM, Christophe Leterrier <
> [hidden email]> wrote:
>
> > *****
> > To join, leave or search the confocal microscopy listserv, go to:
> > http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> > Post images on http://www.imgur.com and include the link in your
> posting.
> > *****
> >
> > Hi,
> >
> > We are looking for 18 mm round coverslips #1.5H (170 ± 5 µm), gridded
> > (photo-etched) for correlative microscopy. Would you know anything
> > approaching (could do with other formats), or a good place to ask to have
> > them custom made?
> >
> > Thank you for your help,
> >
> > Christophe
> >
> > --
> > Christophe Leterrier
> > NeuroCyto lab
> > NICN CNRS UMR 7259
> > Aix Marseille University, France
> >
>

--
Damir Sudar - Affiliate Staff Scientist
Lawrence Berkeley National Laboratory
One Cyclotron Road, MS 977, Berkeley, CA 94720, USA
T: 510/486-5346 - F: 510/486-5586 - E: [hidden email]

Visiting Scientist, Oregon Health and Science University