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Hello everyone,
I am looking for papers that have evaluated the degree of toxicity of
LysoSensor and LysoTracker Probes. I was unable to find details on the
product pages or the references they mention. I have not yet used these
probes, but I am aware that the mechanism of action is that they work by
being protonated and retained in lysosomes. I have been told that these
probes can exhibit an alkalinizing effect on the lysosomes, such that
longer incubations increase pH and lower total lysosomal capacity.
Does anyone have references they have turned to in the past that elucidate
the mechanism of LysoSensor and LysoTracker Probes and the toxic effects of
prolonged use in live cell imaging? I'd be very grateful if you could
direct me to author(s) or papers that have studied this.
Many thanks,
-Feriel
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