MMI cell picker consummables

[Sylvie Le Guyader]

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Dear all

We have an MMI laser microdissection and cell picker system. It works well and we are happy with it but the consumables are outrageously expensive.
I would especially like to find an alternative to buying MMI’s glass capillaries for the cell picker (40€ each capillary!!).
One option is to buy a glass capillary puller and a microforge to smoothen the end.

The capillaries we buy have an inner diameter of 40 um and an outer diameter of 1mm. They have no inner glass filament.
The tough bit is that the end of the capillary is forged so that it is bevelled at 45 degrees to the capillary axis. I need to find a microforge that can bevel our capillaries.

Does anyone (maybe in the zebrafish community?) know about such a microforge?

Thanks! :)

Med vänlig hälsning / Best regards

Sylvie

@@@@@@@@@@@@@@@@@@@@@@@@
Sylvie Le Guyader, PhD
Live Cell Imaging Facility Manager
Karolinska Institutet- Bionut Dpt
Hälsovägen 7C,
Room 7362 (lab)/7840 (office)
14157 Huddinge, Sweden
mobile: [phone]
LCI website<https://ki.se/en/bionut/welcome-to-the-lci-facility>
Follow our microscopy blog!<http://microscopykarolinska.se/>

[Ben Abrams]

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Hi Sylvie,

I recommend talking to Adair Oesterle at Sutter Instruments
https://www.sutter.com/ , she is a wealth of knowledge about this and
has been great for our users.  Good luck.

-Ben

On 10/9/18 5:00 AM, Sylvie Le Guyader wrote:

> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> Post images on http://www.imgur.com and include the link in your posting.
> *****
>
> Dear all
>
> We have an MMI laser microdissection and cell picker system. It works well and we are happy with it but the consumables are outrageously expensive.
> I would especially like to find an alternative to buying MMI’s glass capillaries for the cell picker (40€ each capillary!!).
> One option is to buy a glass capillary puller and a microforge to smoothen the end.
>
> The capillaries we buy have an inner diameter of 40 um and an outer diameter of 1mm. They have no inner glass filament.
> The tough bit is that the end of the capillary is forged so that it is bevelled at 45 degrees to the capillary axis. I need to find a microforge that can bevel our capillaries.
>
> Does anyone (maybe in the zebrafish community?) know about such a microforge?
>
> Thanks! :)
>
> Med vänlig hälsning / Best regards
>
> Sylvie
>
> @@@@@@@@@@@@@@@@@@@@@@@@
> Sylvie Le Guyader, PhD
> Live Cell Imaging Facility Manager
> Karolinska Institutet- Bionut Dpt
> Hälsovägen 7C,
> Room 7362 (lab)/7840 (office)
> 14157 Huddinge, Sweden
> mobile: [phone]
> LCI website<https://ki.se/en/bionut/welcome-to-the-lci-facility>
> Follow our microscopy blog!<http://microscopykarolinska.se/>
>
>

--
Benjamin Abrams,  Ph.D.
Facility Manager
UCSC Life Sciences Microscopy Center

University of California, Santa Cruz
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150 Sinsheimer labs
Santa Cruz, CA 95064

Office/voicemail: [phone]
Mobile: [phone]

http://stemcell.soe.ucsc.edu/facilities/microscopy

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