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MRC 1024 question

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Stephen C. Kempf Stephen C. Kempf
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MRC 1024 question

We're having a problem with our BioRad MRC 1024 confocal that I hope someone else on this list has experienced and resolved. When using the scope a week or so ago, it stopped producing images and started giving solid green, red or blue scans on the various channels. A few days later, one of my other students used it and it was working properly again. Then this weekend, we got the solid green, red or blue scans again. We tried various changes in settings, different accounts, different methods, retarting the software, and restarting the system, but to no avail. Can any one offer some insight as to what the problem might be?

Thank you,

Steve Kempf
______________________________________________________________________________
Stephen C. Kempf E-mail: [hidden email]
Associate Professor Tel:  334-844-3924
Faculty Director, AU Hybridoma Facility Cell: 334-703-6689
Department of Biological Sciences Fax: 334-844-9234
331 Funchess Hall
Auburn University, AL  36849
Course web sites:
Nuno Moreno Nuno Moreno
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Re: MRC 1024 question

Welcome to the world of problems with pre-amps. The similar problems I
had up to know are related with it.


Regards,
NM



Stephen C. Kempf wrote:

> We're having a problem with our BioRad MRC 1024 confocal that I hope
> someone else on this list has experienced and resolved. When using the
> scope a week or so ago, it stopped producing images and started giving
> solid green, red or blue scans on the various channels. A few days
> later, one of my other students used it and it was working properly
> again. Then this weekend, we got the solid green, red or blue scans
> again. We tried various changes in settings, different accounts,
> different methods, retarting the software, and restarting the system,
> but to no avail. Can any one offer some insight as to what the problem
> might be?
>
> Thank you,
>
> Steve Kempf
> ______________________________________________________________________________
> Stephen C. Kempf E-mail: [hidden email] <mailto:[hidden email]>
> Associate Professor Tel:  334-844-3924
> Faculty Director, AU Hybridoma Facility Cell: 334-703-6689
> Department of Biological Sciences Fax: 334-844-9234
> 331 Funchess Hall
> Auburn University, AL  36849
> *Lab Web site* -
> http://www.auburn.edu/cosam/departments/biology/faculty/webpages/kempf/index.htm
> *Course web sites:*
> *   Vert.
> Dev.* - http://www.auburn.edu/academic/classes/zy/vert_embryo/index_vert_embryo.html
> *  
> Histology*  - http://www.auburn.edu/academic/classes/zy/hist0509/index_histology.html 
> <http://www.auburn.edu/academic/classes/zy/hist0509/index_histologyhtml>
>
>
>
Moninger, Thomas Moninger, Thomas
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Re: MRC 1024 question

In reply to this post by Stephen C. Kempf

Steve,

 

Well, let’s check the easiest possibility first. Could it be that the software is occasionally being started in the “No hardware” or emulation mode? What you describe is exactly what happens when you try to scan in emulation mode.

 

Tom

 

Notice: This UI Health Care e-mail (including attachments) is covered by the Electronic Communications Privacy Act, 18 U.S.C. 2510-2521, is confidential and may be legally privileged.  If you are not the intended recipient, you are hereby notified that any retention, dissemination, distribution, or copying of this communication is strictly prohibited.  Please reply to the sender that you have received the message in error, then delete it.  Thank you.

From: Confocal Microscopy List [mailto:[hidden email]] On Behalf Of Stephen C. Kempf
Sent: Wednesday, March 04, 2009 10:44 AM
To: [hidden email]
Subject: MRC 1024 question

 

We're having a problem with our BioRad MRC 1024 confocal that I hope someone else on this list has experienced and resolved. When using the scope a week or so ago, it stopped producing images and started giving solid green, red or blue scans on the various channels. A few days later, one of my other students used it and it was working properly again. Then this weekend, we got the solid green, red or blue scans again. We tried various changes in settings, different accounts, different methods, retarting the software, and restarting the system, but to no avail. Can any one offer some insight as to what the problem might be?

 

Thank you,

 

Steve Kempf

______________________________________________________________________________

Stephen C. Kempf                                                                                               E-mail: [hidden email]

Associate Professor                                                                                           Tel:  334-844-3924

Faculty Director, AU Hybridoma Facility                          Cell: 334-703-6689

Department of Biological Sciences                                 Fax: 334-844-9234

331 Funchess Hall

Auburn University, AL  36849

Course web sites:

 

 

 
Stephen C. Kempf Stephen C. Kempf
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Re: MRC 1024 question

Tom,

I wish it was that simple. The system is running with the scanning software (not emulation) and one of my students just told me that it happened in the middle of a scan that was progressing normally the other day. Seems to me like it might be a hardware problem.

Steve

On Mar 4, 2009, at 10:57 AM, Moninger, Thomas wrote:

Steve,

 

Well, let’s check the easiest possibility first. Could it be that the software is occasionally being started in the “No hardware” or emulation mode? What you describe is exactly what happens when you try to scan in emulation mode.

 

Tom

 

Notice: This UI Health Care e-mail (including attachments) is covered by the Electronic Communications Privacy Act, 18 U.S.C. 2510-2521, is confidential and may be legally privileged.  If you are not the intended recipient, you are hereby notified that any retention, dissemination, distribution, or copying of this communication is strictly prohibited.  Please reply to the sender that you have received the message in error, then delete it.  Thank you.

From: Confocal Microscopy List [[hidden email]] On Behalf Of Stephen C. Kempf
Sent: Wednesday, March 04, 2009 10:44 AM
To: [hidden email]
Subject: MRC 1024 question

 

We're having a problem with our BioRad MRC 1024 confocal that I hope someone else on this list has experienced and resolved. When using the scope a week or so ago, it stopped producing images and started giving solid green, red or blue scans on the various channels. A few days later, one of my other students used it and it was working properly again. Then this weekend, we got the solid green, red or blue scans again. We tried various changes in settings, different accounts, different methods, retarting the software, and restarting the system, but to no avail. Can any one offer some insight as to what the problem might be?

 

Thank you,

 

Steve Kempf

______________________________________________________________________________

Stephen C. Kempf                                                                                               E-mail: [hidden email]

Associate Professor                                                                                           Tel:  334-844-3924

Faculty Director, AU Hybridoma Facility                          Cell: 334-703-6689

Department of Biological Sciences                                 Fax: 334-844-9234

331 Funchess Hall

Auburn University, AL  36849

Course web sites:

 

 

 
George Ring George Ring
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Re: MRC 1024 question

Steve,

First of all, I have no idea what is causing your problem.  You seem to
have adopted the rule of thumb "When in doubt, reboot".  With a different
problem that we've experienced on our MRC1024, we found that rebooting
wasn't sufficient.  In order to "reboot" the system, we have to physically
unplug the control tower.  Even when it is off, it is still receiving power
and doesn't reset by simply switching it off.

Good luck,

George


George Ring, Ph.D.
Dept. of Cell and Developmental Biology
SUNY Upstate Medical University
750 E. Adams St.
Syracuse NY  13210
Tel. (315) 464-8595
FAX (315) 464-8535
email: [hidden email]





>>> On Wed, Mar 4, 2009 at  1:38 PM, in message
<[hidden email]>, "Stephen C. Kempf"
<[hidden email]> wrote:

> Tom,
>
> I wish it was that simple. The system is running with the scanning  
> software (not emulation) and one of my students just told me that it  
> happened in the middle of a scan that was progressing normally the  
> other day. Seems to me like it might be a hardware problem.
>
> Steve
>
> On Mar 4, 2009, at 10:57 AM, Moninger, Thomas wrote:
>
>> Steve,
>>
>> Well, let*s check the easiest possibility first. Could it be that  
>> the software is occasionally being started in the *No hardware* or

>> emulation mode? What you describe is exactly what happens when you  
>> try to scan in emulation mode.
>>
>> Tom
>>
>> Notice: This UI Health Care e-mail (including attachments) is  
>> covered by the Electronic Communications Privacy Act, 18 U.S.C.  
>> 2510-2521, is confidential and may be legally privileged.  If you  
>> are not the intended recipient, you are hereby notified that any  
>> retention, dissemination, distribution, or copying of this  
>> communication is strictly prohibited.  Please reply to the sender  
>> that you have received the message in error, then delete it.  Thank  
>> you.
>>
>> From: Confocal Microscopy List  
>> [mailto:[hidden email]] On Behalf Of Stephen C.  
>> Kempf
>> Sent: Wednesday, March 04, 2009 10:44 AM
>> To: [hidden email]
>> Subject: MRC 1024 question
>>
>> We're having a problem with our BioRad MRC 1024 confocal that I  
>> hope someone else on this list has experienced and resolved. When  
>> using the scope a week or so ago, it stopped producing images and  
>> started giving solid green, red or blue scans on the various  
>> channels. A few days later, one of my other students used it and it  
>> was working properly again. Then this weekend, we got the solid  
>> green, red or blue scans again. We tried various changes in  
>> settings, different accounts, different methods, retarting the  
>> software, and restarting the system, but to no avail. Can any one  
>> offer some insight as to what the problem might be?
>>
>> Thank you,
>>
>> Steve Kempf
>> ______________________________________________________________________
>> ________
>> Stephen C.  
>> Kempf                                                                  
>>                               E-mail: [hidden email]
>> Associate  
>> Professor                                                              
>>                               Tel:  334-844-3924
>> Faculty Director, AU Hybridoma Facility                          
>> Cell: 334-703-6689
>> Department of Biological Sciences                                  
>> Fax: 334-844-9234
>> 331 Funchess Hall
>> Auburn University, AL  36849
>> Lab Web site - http://www.auburn.edu/cosam/departments/biology/ 
>> faculty/webpages/kempf/index.htm
>> Course web sites:
>>    Vert. Dev. - http://www.auburn.edu/academic/classes/zy/ 
>> vert_embryo/index_vert_embryo.html
>>    Histology  - http://www.auburn.edu/academic/classes/zy/hist0509/ 
>> index_histology.html
>>
>>
>>
>>
Renato A. Mortara Renato A. Mortara
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RES: MRC 1024 question

Steve,

This is general reply. Besides the VIS board issue, you may also try to
re-seat all the boards in the computer.

Renato

 

-----Mensagem original-----
De: Confocal Microscopy List [mailto:[hidden email]] Em
nome de George Ring
Enviada em: quarta-feira, 4 de março de 2009 15:52
Para: [hidden email]
Assunto: Re: MRC 1024 question

Steve,

First of all, I have no idea what is causing your problem.  You seem to have
adopted the rule of thumb "When in doubt, reboot".  With a different problem
that we've experienced on our MRC1024, we found that rebooting wasn't
sufficient.  In order to "reboot" the system, we have to physically unplug
the control tower.  Even when it is off, it is still receiving power and
doesn't reset by simply switching it off.

Good luck,

George


George Ring, Ph.D.
Dept. of Cell and Developmental Biology
SUNY Upstate Medical University
750 E. Adams St.
Syracuse NY  13210
Tel. (315) 464-8595
FAX (315) 464-8535
email: [hidden email]





>>> On Wed, Mar 4, 2009 at  1:38 PM, in message
<[hidden email]>, "Stephen C. Kempf"
<[hidden email]> wrote:

> Tom,
>
> I wish it was that simple. The system is running with the scanning
> software (not emulation) and one of my students just told me that it
> happened in the middle of a scan that was progressing normally the
> other day. Seems to me like it might be a hardware problem.
>
> Steve
>
> On Mar 4, 2009, at 10:57 AM, Moninger, Thomas wrote:
>
>> Steve,
>>
>> Well, let*s check the easiest possibility first. Could it be that the
>> software is occasionally being started in the *No hardware* or

>> emulation mode? What you describe is exactly what happens when you
>> try to scan in emulation mode.
>>
>> Tom
>>
>> Notice: This UI Health Care e-mail (including attachments) is covered
>> by the Electronic Communications Privacy Act, 18 U.S.C.
>> 2510-2521, is confidential and may be legally privileged.  If you are
>> not the intended recipient, you are hereby notified that any
>> retention, dissemination, distribution, or copying of this
>> communication is strictly prohibited.  Please reply to the sender
>> that you have received the message in error, then delete it.  Thank
>> you.
>>
>> From: Confocal Microscopy List
>> [mailto:[hidden email]] On Behalf Of Stephen C.  
>> Kempf
>> Sent: Wednesday, March 04, 2009 10:44 AM
>> To: [hidden email]
>> Subject: MRC 1024 question
>>
>> We're having a problem with our BioRad MRC 1024 confocal that I hope
>> someone else on this list has experienced and resolved. When using
>> the scope a week or so ago, it stopped producing images and started
>> giving solid green, red or blue scans on the various channels. A few
>> days later, one of my other students used it and it was working
>> properly again. Then this weekend, we got the solid green, red or
>> blue scans again. We tried various changes in settings, different
>> accounts, different methods, retarting the software, and restarting
>> the system, but to no avail. Can any one offer some insight as to
>> what the problem might be?
>>
>> Thank you,
>>
>> Steve Kempf
>> _____________________________________________________________________
>> _
>> ________
>> Stephen C.  
>> Kempf                                                                  
>>                               E-mail: [hidden email] Associate
>> Professor                                                              
>>                               Tel:  334-844-3924
>> Faculty Director, AU Hybridoma Facility                          
>> Cell: 334-703-6689
>> Department of Biological Sciences                                  
>> Fax: 334-844-9234
>> 331 Funchess Hall
>> Auburn University, AL  36849
>> Lab Web site - http://www.auburn.edu/cosam/departments/biology/
>> faculty/webpages/kempf/index.htm
>> Course web sites:
>>    Vert. Dev. - http://www.auburn.edu/academic/classes/zy/
>> vert_embryo/index_vert_embryo.html
>>    Histology  - http://www.auburn.edu/academic/classes/zy/hist0509/
>> index_histology.html
>>
>>
>>
>>
Glen MacDonald-2 Glen MacDonald-2
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Re: RES: MRC 1024 question

My general procedure with our MRC-1000/1024 update system has been to  
power down and reseat all boards in the computer.  This has been  
required on 3 different computers.
Try that before digging more deeply into the problem.

Regards,
Glen

Glen MacDonald
Core for Communication Research
Virginia Merrill Bloedel Hearing Research Center
Box 357923
University of Washington
Seattle, WA 98195-7923  USA
(206) 616-4156
[hidden email]

******************************************************************************
The box said "Requires WindowsXP or better", so I bought a Macintosh.
******************************************************************************


On Mar 4, 2009, at 11:03 AM, Renato Mortara wrote:

> Steve,
>
> This is general reply. Besides the VIS board issue, you may also try  
> to
> re-seat all the boards in the computer.
>
> Renato
>
>
>
> -----Mensagem original-----
> De: Confocal Microscopy List  
> [mailto:[hidden email]] Em
> nome de George Ring
> Enviada em: quarta-feira, 4 de março de 2009 15:52
> Para: [hidden email]
> Assunto: Re: MRC 1024 question
>
> Steve,
>
> First of all, I have no idea what is causing your problem.  You seem  
> to have
> adopted the rule of thumb "When in doubt, reboot".  With a different  
> problem
> that we've experienced on our MRC1024, we found that rebooting wasn't
> sufficient.  In order to "reboot" the system, we have to physically  
> unplug
> the control tower.  Even when it is off, it is still receiving power  
> and
> doesn't reset by simply switching it off.
>
> Good luck,
>
> George
>
>
> George Ring, Ph.D.
> Dept. of Cell and Developmental Biology
> SUNY Upstate Medical University
> 750 E. Adams St.
> Syracuse NY  13210
> Tel. (315) 464-8595
> FAX (315) 464-8535
> email: [hidden email]
>
>
>
>
>
>>>> On Wed, Mar 4, 2009 at  1:38 PM, in message
> <[hidden email]>, "Stephen C. Kempf"
> <[hidden email]> wrote:
>> Tom,
>>
>> I wish it was that simple. The system is running with the scanning
>> software (not emulation) and one of my students just told me that it
>> happened in the middle of a scan that was progressing normally the
>> other day. Seems to me like it might be a hardware problem.
>>
>> Steve
>>
>> On Mar 4, 2009, at 10:57 AM, Moninger, Thomas wrote:
>>
>>> Steve,
>>>
>>> Well, let*s check the easiest possibility first. Could it be that  
>>> the
>>> software is occasionally being started in the *No hardware* or
>
>>> emulation mode? What you describe is exactly what happens when you
>>> try to scan in emulation mode.
>>>
>>> Tom
>>>
>>> Notice: This UI Health Care e-mail (including attachments) is  
>>> covered
>>> by the Electronic Communications Privacy Act, 18 U.S.C.
>>> 2510-2521, is confidential and may be legally privileged.  If you  
>>> are
>>> not the intended recipient, you are hereby notified that any
>>> retention, dissemination, distribution, or copying of this
>>> communication is strictly prohibited.  Please reply to the sender
>>> that you have received the message in error, then delete it.  Thank
>>> you.
>>>
>>> From: Confocal Microscopy List
>>> [mailto:[hidden email]] On Behalf Of Stephen C.
>>> Kempf
>>> Sent: Wednesday, March 04, 2009 10:44 AM
>>> To: [hidden email]
>>> Subject: MRC 1024 question
>>>
>>> We're having a problem with our BioRad MRC 1024 confocal that I hope
>>> someone else on this list has experienced and resolved. When using
>>> the scope a week or so ago, it stopped producing images and started
>>> giving solid green, red or blue scans on the various channels. A few
>>> days later, one of my other students used it and it was working
>>> properly again. Then this weekend, we got the solid green, red or
>>> blue scans again. We tried various changes in settings, different
>>> accounts, different methods, retarting the software, and restarting
>>> the system, but to no avail. Can any one offer some insight as to
>>> what the problem might be?
>>>
>>> Thank you,
>>>
>>> Steve Kempf
>>> _____________________________________________________________________
>>> _
>>> ________
>>> Stephen C.
>>> Kempf
>>>                              E-mail: [hidden email] Associate
>>> Professor
>>>                              Tel:  334-844-3924
>>> Faculty Director, AU Hybridoma Facility
>>> Cell: 334-703-6689
>>> Department of Biological Sciences
>>> Fax: 334-844-9234
>>> 331 Funchess Hall
>>> Auburn University, AL  36849
>>> Lab Web site - http://www.auburn.edu/cosam/departments/biology/
>>> faculty/webpages/kempf/index.htm
>>> Course web sites:
>>>   Vert. Dev. - http://www.auburn.edu/academic/classes/zy/
>>> vert_embryo/index_vert_embryo.html
>>>   Histology  - http://www.auburn.edu/academic/classes/zy/hist0509/
>>> index_histology.html
>>>
>>>
>>>
>>>
confocalservice confocalservice
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Re: RES: MRC 1024 question

Hi Steve,
 
as I understand this is a NT upgraded, are you still using ISA card or that has been updated to new PCI version?
it seems VIS board is faulty and as some suggested try to clean connectors (use ethanol to clean them) and resit them , it may work
 
farhad
confocalservice

2009/3/4 Glen MacDonald <[hidden email]>
My general procedure with our MRC-1000/1024 update system has been to power down and reseat all boards in the computer.  This has been required on 3 different computers.
Try that before digging more deeply into the problem.

Regards,
Glen

Glen MacDonald
Core for Communication Research
Virginia Merrill Bloedel Hearing Research Center
Box 357923
University of Washington
Seattle, WA 98195-7923  USA
(206) 616-4156
[hidden email]

******************************************************************************
The box said "Requires WindowsXP or better", so I bought a Macintosh.
******************************************************************************



On Mar 4, 2009, at 11:03 AM, Renato Mortara wrote:

Steve,

This is general reply. Besides the VIS board issue, you may also try to
re-seat all the boards in the computer.

Renato



-----Mensagem original-----
De: Confocal Microscopy List [mailto:[hidden email]] Em
nome de George Ring
Enviada em: quarta-feira, 4 de março de 2009 15:52
Para: [hidden email]
Assunto: Re: MRC 1024 question

Steve,

First of all, I have no idea what is causing your problem.  You seem to have
adopted the rule of thumb "When in doubt, reboot".  With a different problem
that we've experienced on our MRC1024, we found that rebooting wasn't
sufficient.  In order to "reboot" the system, we have to physically unplug
the control tower.  Even when it is off, it is still receiving power and
doesn't reset by simply switching it off.

Good luck,

George


George Ring, Ph.D.
Dept. of Cell and Developmental Biology
SUNY Upstate Medical University
750 E. Adams St.
Syracuse NY  13210
Tel. (315) 464-8595
FAX (315) 464-8535
email: [hidden email]





On Wed, Mar 4, 2009 at  1:38 PM, in message
<[hidden email]>, "Stephen C. Kempf"
<[hidden email]> wrote:
Tom,

I wish it was that simple. The system is running with the scanning
software (not emulation) and one of my students just told me that it
happened in the middle of a scan that was progressing normally the
other day. Seems to me like it might be a hardware problem.

Steve

On Mar 4, 2009, at 10:57 AM, Moninger, Thomas wrote:

Steve,

Well, let*s check the easiest possibility first. Could it be that the
software is occasionally being started in the *No hardware* or

emulation mode? What you describe is exactly what happens when you
try to scan in emulation mode.

Tom

Notice: This UI Health Care e-mail (including attachments) is covered
by the Electronic Communications Privacy Act, 18 U.S.C.
2510-2521, is confidential and may be legally privileged.  If you are
not the intended recipient, you are hereby notified that any
retention, dissemination, distribution, or copying of this
communication is strictly prohibited.  Please reply to the sender
that you have received the message in error, then delete it.  Thank
you.

From: Confocal Microscopy List
[mailto:[hidden email]] On Behalf Of Stephen C.
Kempf
Sent: Wednesday, March 04, 2009 10:44 AM
To: [hidden email]
Subject: MRC 1024 question

We're having a problem with our BioRad MRC 1024 confocal that I hope
someone else on this list has experienced and resolved. When using
the scope a week or so ago, it stopped producing images and started
giving solid green, red or blue scans on the various channels. A few
days later, one of my other students used it and it was working
properly again. Then this weekend, we got the solid green, red or
blue scans again. We tried various changes in settings, different
accounts, different methods, retarting the software, and restarting
the system, but to no avail. Can any one offer some insight as to
what the problem might be?

Thank you,

Steve Kempf
_____________________________________________________________________
_
________
Stephen C.
Kempf
                            E-mail: [hidden email] Associate
Professor
                            Tel:  334-844-3924
Faculty Director, AU Hybridoma Facility
Cell: 334-703-6689
Department of Biological Sciences
Fax: 334-844-9234
331 Funchess Hall
Auburn University, AL  36849
Lab Web site - http://www.auburn.edu/cosam/departments/biology/
faculty/webpages/kempf/index.htm
Course web sites:
 Vert. Dev. - http://www.auburn.edu/academic/classes/zy/
vert_embryo/index_vert_embryo.html
 Histology  - http://www.auburn.edu/academic/classes/zy/hist0509/
index_histology.html
Jeff Reece Jeff Reece
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Re: MRC 1024 question

In reply to this post by Stephen C. Kempf
 
Hi Steve,
 
Could be heat-related.  Check all the fans in the electronics, vacuum out any dust, keep the ventilation openings clear.
 
Best,
Jeff

--------------------
Jeff M. Reece
Reecent Technologies, LLC
“Honing the edge of quantitative microscopy”

[hidden email]

Toll-free: 877-217-8465

(direct line: 919-672-4681)

www.reecent.com

612 ½ Center Street Ext
Meadville, PA 16335-2452



----- Original Message -----
Sent: Wednesday, March 04, 2009 1:38 PM
Subject: Re: MRC 1024 question

Tom,

I wish it was that simple. The system is running with the scanning software (not emulation) and one of my students just told me that it happened in the middle of a scan that was progressing normally the other day. Seems to me like it might be a hardware problem.

Steve

On Mar 4, 2009, at 10:57 AM, Moninger, Thomas wrote:

Steve,

Well, let’s check the easiest possibility first. Could it be that the software is occasionally being started in the “No hardware” or emulation mode? What you describe is exactly what happens when you try to scan in emulation mode.

Tom

Notice: This UI Health Care e-mail (including attachments) is covered by the Electronic Communications Privacy Act, 18 U.S.C. 2510-2521, is confidential and may be legally privileged.  If you are not the intended recipient, you are hereby notified that any retention, dissemination, distribution, or copying of this communication is strictly prohibited.  Please reply to the sender that you have received the message in error, then delete it.  Thank you.

From: Confocal Microscopy List [[hidden email]] On Behalf Of Stephen C. Kempf
Sent: Wednesday, March 04, 2009 10:44 AM
To: [hidden email]
Subject: MRC 1024 question

We're having a problem with our BioRad MRC 1024 confocal that I hope someone else on this list has experienced and resolved. When using the scope a week or so ago, it stopped producing images and started giving solid green, red or blue scans on the various channels. A few days later, one of my other students used it and it was working properly again. Then this weekend, we got the solid green, red or blue scans again. We tried various changes in settings, different accounts, different methods, retarting the software, and restarting the system, but to no avail. Can any one offer some insight as to what the problem might be?

Thank you,

Steve Kempf

______________________________________________________________________________

Stephen C. Kempf                                                                                               E-mail: [hidden email]

Associate Professor                                                                                           Tel:  334-844-3924

Faculty Director, AU Hybridoma Facility                          Cell: 334-703-6689

Department of Biological Sciences                                 Fax: 334-844-9234

331 Funchess Hall

Auburn University, AL  36849

Course web sites:
Arvydas Matiukas Arvydas Matiukas
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Axiovert 200M question

In reply to this post by George Ring
Hi all,
 
We are having a problem with our Zeiss Axiovert 200M. It stopped
responding to any buttons. Fuses are OK, and it is getting power.
On the back red LED is on (as well as green one), and LCD screen
is showing squares. Can anyone  offer some insight as to what the
problem might be? LSM software is showing error messages for
COM1 but computer is OK so I assume the problem is on the scope
side.
Thank you,
Arvydas

 
 
Arvydas Matiukas, Ph.D.
Director of Confocal&Two-Photon Imaging Core Facility
Department of Pharmacology
SUNY Upstate Medical University
766 Irving Ave., WH 3159
Syracuse, NY 13210
tel.: 315-464-7997
fax: 315-464-8014
email: [hidden email]
Steffen Dietzel Steffen Dietzel
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Re: Axiovert 200M question

Hi Arvydas,

sounds like a controler board went down. To make sure the problem is
on the scope side, I'd suggest you unplug all cables that lead to the
computer or other electronics and restart the scope. If the problem
persists, that'll be a pretty clear diagnosis.

In a next step, I' d unplug cables between the stand and electronic
condensors, remove filter wheel etc, until you have only the naked
stand left. It might be that the stand electronic is waiting for a
response that is not coming. Else, it is the electronic in the stand itself.

Obviously, you'd better change things only when power is off or you
might end up with more trouble than you start with.

Good bug hunting

Steffen


At 23:05 04.03.2009, you wrote:

>Hi all,
>
>We are having a problem with our Zeiss Axiovert 200M. It stopped
>responding to any buttons. Fuses are OK, and it is getting power.
>On the back red LED is on (as well as green one), and LCD screen
>is showing squares. Can anyone  offer some insight as to what the
>problem might be? LSM software is showing error messages for
>COM1 but computer is OK so I assume the problem is on the scope
>side.
>Thank you,
>Arvydas
>
>
>
>Arvydas Matiukas, Ph.D.
>Director of Confocal&Two-Photon Imaging Core Facility
>Department of Pharmacology
>SUNY Upstate Medical University
>766 Irving Ave., WH 3159
>Syracuse, NY 13210
>tel.: 315-464-7997
>fax: 315-464-8014
>email: [hidden email]
Stephen C. Kempf Stephen C. Kempf
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Re: MRC 1024 question

In reply to this post by Jeff Reece
Just wanted to thank everyone for all the advice about possible solutions to our problem. It turns out our microscopy facility will be undergoing renovations for the next week or so. Once that's completed and I can get to the scope again, I'll try what people suggested and get back to the list about the results.

Thanks again,

Steve
______________________________________________________________________________
Stephen C. Kempf E-mail: [hidden email]
Associate Professor Tel:  334-844-3924
Faculty Director, AU Hybridoma Facility Cell: 334-703-6689
Department of Biological Sciences Fax: 334-844-9234
331 Funchess Hall
Auburn University, AL  36849
Course web sites:



On Mar 4, 2009, at 2:28 PM, Jeff Reece wrote:

 
Hi Steve,
 
Could be heat-related.  Check all the fans in the electronics, vacuum out any dust, keep the ventilation openings clear.
 
Best,
Jeff

--------------------
Jeff M. Reece
Reecent Technologies, LLC
“Honing the edge of quantitative microscopy”

Toll-free: 877-217-8465
(direct line: 919-672-4681)

612 ½ Center Street Ext
Meadville, PA 16335-2452



----- Original Message -----
Sent: Wednesday, March 04, 2009 1:38 PM
Subject: Re: MRC 1024 question

Tom,

I wish it was that simple. The system is running with the scanning software (not emulation) and one of my students just told me that it happened in the middle of a scan that was progressing normally the other day. Seems to me like it might be a hardware problem.

Steve

On Mar 4, 2009, at 10:57 AM, Moninger, Thomas wrote:

Steve,

Well, let’s check the easiest possibility first. Could it be that the software is occasionally being started in the “No hardware” or emulation mode? What you describe is exactly what happens when you try to scan in emulation mode.

Tom

Notice: This UI Health Care e-mail (including attachments) is covered by the Electronic Communications Privacy Act, 18 U.S.C. 2510-2521, is confidential and may be legally privileged.  If you are not the intended recipient, you are hereby notified that any retention, dissemination, distribution, or copying of this communication is strictly prohibited.  Please reply to the sender that you have received the message in error, then delete it.  Thank you.

From: Confocal Microscopy List [[hidden email]] On Behalf Of Stephen C. Kempf
Sent: Wednesday, March 04, 2009 10:44 AM
To: [hidden email]
Subject: MRC 1024 question

We're having a problem with our BioRad MRC 1024 confocal that I hope someone else on this list has experienced and resolved. When using the scope a week or so ago, it stopped producing images and started giving solid green, red or blue scans on the various channels. A few days later, one of my other students used it and it was working properly again. Then this weekend, we got the solid green, red or blue scans again. We tried various changes in settings, different accounts, different methods, retarting the software, and restarting the system, but to no avail. Can any one offer some insight as to what the problem might be?

Thank you,

Steve Kempf

______________________________________________________________________________

Stephen C. Kempf                                                                                               E-mail: [hidden email]

Associate Professor                                                                                           Tel:  334-844-3924

Faculty Director, AU Hybridoma Facility                          Cell: 334-703-6689

Department of Biological Sciences                                 Fax: 334-844-9234

331 Funchess Hall

Auburn University, AL  36849

Course web sites:
Catherine GARNIER Catherine GARNIER
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components of Zeiss LSM 410

Hi,

We have used since 1995  a Zeiss LSM 410 Confocal microscope. We have tried to update it, but the Zeiss service decided to stop the service (which was already not really as we wanted it to be..).

So, we have 3 lasers, changed in June 2004,  used around 1500 h:
        - Laser Argon référence Zeiss 000000 0234 267
        - Laser He Ne 543 nm référence Zeiss 577009 0778 400
        - Laser He Ne 633 nm référence Zeiss 577009 0709 100

2 PMTs,  changed in June 2006 :
    - PMT  N°R4632 (Hamamatsu)
    - PMT  N°R3896 (Hamamatsu)

and the anti-vibration  table 193 x 123 x 88 cm (L x l x h)

If someone is interested by one or more of these equipments, or other electronic components, please contact me directly.

Best regards

Catherine

[hidden email] 
-- 
**************************************************************************
Dr Catherine Garnier
Institut National de la Recherche Agronomique - INRA
Biopolymères, Interactions, Assemblages - BIA
Interfaces et Systèmes Dispersés - ISD
Rue de la Géraudière - BP 71627 - 44316 Nantes cedex 3
Tel : 33 (0)2 40 67 50 45
Fax: 33 (0)2 40 67 50 84
http://www.nantes.inra.fr
**************************************************************************
Stephen C. Kempf Stephen C. Kempf
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Re: MRC 1024 question

In reply to this post by Jeff Reece
Jeff,

Thanks for your suggestions. Once I can get to the scope again, I'll give your suggestions a try.

Steve
______________________________________________________________________________
Stephen C. Kempf E-mail: [hidden email]
Associate Professor Tel:  334-844-3924
Faculty Director, AU Hybridoma Facility Cell: 334-703-6689
Department of Biological Sciences Fax: 334-844-9234
331 Funchess Hall
Auburn University, AL  36849
Course web sites:


On Mar 4, 2009, at 2:28 PM, Jeff Reece wrote:

 
Hi Steve,
 
Could be heat-related.  Check all the fans in the electronics, vacuum out any dust, keep the ventilation openings clear.
 
Best,
Jeff

--------------------
Jeff M. Reece
Reecent Technologies, LLC
“Honing the edge of quantitative microscopy”

Toll-free: 877-217-8465
(direct line: 919-672-4681)

612 ½ Center Street Ext
Meadville, PA 16335-2452



----- Original Message -----
Sent: Wednesday, March 04, 2009 1:38 PM
Subject: Re: MRC 1024 question

Tom,

I wish it was that simple. The system is running with the scanning software (not emulation) and one of my students just told me that it happened in the middle of a scan that was progressing normally the other day. Seems to me like it might be a hardware problem.

Steve

On Mar 4, 2009, at 10:57 AM, Moninger, Thomas wrote:

Steve,

Well, let’s check the easiest possibility first. Could it be that the software is occasionally being started in the “No hardware” or emulation mode? What you describe is exactly what happens when you try to scan in emulation mode.

Tom

Notice: This UI Health Care e-mail (including attachments) is covered by the Electronic Communications Privacy Act, 18 U.S.C. 2510-2521, is confidential and may be legally privileged.  If you are not the intended recipient, you are hereby notified that any retention, dissemination, distribution, or copying of this communication is strictly prohibited.  Please reply to the sender that you have received the message in error, then delete it.  Thank you.

From: Confocal Microscopy List [[hidden email]] On Behalf Of Stephen C. Kempf
Sent: Wednesday, March 04, 2009 10:44 AM
To: [hidden email]
Subject: MRC 1024 question

We're having a problem with our BioRad MRC 1024 confocal that I hope someone else on this list has experienced and resolved. When using the scope a week or so ago, it stopped producing images and started giving solid green, red or blue scans on the various channels. A few days later, one of my other students used it and it was working properly again. Then this weekend, we got the solid green, red or blue scans again. We tried various changes in settings, different accounts, different methods, retarting the software, and restarting the system, but to no avail. Can any one offer some insight as to what the problem might be?

Thank you,

Steve Kempf

______________________________________________________________________________

Stephen C. Kempf                                                                                               E-mail: [hidden email]

Associate Professor                                                                                           Tel:  334-844-3924

Faculty Director, AU Hybridoma Facility                          Cell: 334-703-6689

Department of Biological Sciences                                 Fax: 334-844-9234

331 Funchess Hall

Auburn University, AL  36849

Course web sites:
Stephen C. Kempf Stephen C. Kempf
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Re: RES: MRC 1024 question

In reply to this post by Glen MacDonald-2
Glen,

Thanks for your suggestions. Once I can get to the scope again, I'll give your suggestions a try.

Steve
______________________________________________________________________________
Stephen C. Kempf E-mail: [hidden email]
Associate Professor Tel:  334-844-3924
Faculty Director, AU Hybridoma Facility Cell: 334-703-6689
Department of Biological Sciences Fax: 334-844-9234
331 Funchess Hall
Auburn University, AL  36849
Course web sites:


On Mar 4, 2009, at 1:09 PM, Glen MacDonald wrote:

My general procedure with our MRC-1000/1024 update system has been to power down and reseat all boards in the computer.  This has been required on 3 different computers.
Try that before digging more deeply into the problem.

Regards,
Glen

Glen MacDonald
Core for Communication Research
Virginia Merrill Bloedel Hearing Research Center
Box 357923
University of Washington
Seattle, WA 98195-7923  USA
(206) 616-4156

******************************************************************************
The box said "Requires WindowsXP or better", so I bought a Macintosh.
******************************************************************************


On Mar 4, 2009, at 11:03 AM, Renato Mortara wrote:

Steve,

This is general reply. Besides the VIS board issue, you may also try to
re-seat all the boards in the computer.

Renato



-----Mensagem original-----
De: Confocal Microscopy List [[hidden email]] Em
nome de George Ring
Enviada em: quarta-feira, 4 de março de 2009 15:52
Assunto: Re: MRC 1024 question

Steve,

First of all, I have no idea what is causing your problem.  You seem to have
adopted the rule of thumb "When in doubt, reboot".  With a different problem
that we've experienced on our MRC1024, we found that rebooting wasn't
sufficient.  In order to "reboot" the system, we have to physically unplug
the control tower.  Even when it is off, it is still receiving power and
doesn't reset by simply switching it off.

Good luck,

George


George Ring, Ph.D.
Dept. of Cell and Developmental Biology
SUNY Upstate Medical University
750 E. Adams St.
Syracuse NY  13210
Tel. (315) 464-8595
FAX (315) 464-8535





On Wed, Mar 4, 2009 at  1:38 PM, in message
<[hidden email]>, "Stephen C. Kempf"
Tom,

I wish it was that simple. The system is running with the scanning
software (not emulation) and one of my students just told me that it
happened in the middle of a scan that was progressing normally the
other day. Seems to me like it might be a hardware problem.

Steve

On Mar 4, 2009, at 10:57 AM, Moninger, Thomas wrote:

Steve,

Well, let*s check the easiest possibility first. Could it be that the
software is occasionally being started in the *No hardware* or

emulation mode? What you describe is exactly what happens when you
try to scan in emulation mode.

Tom

Notice: This UI Health Care e-mail (including attachments) is covered
by the Electronic Communications Privacy Act, 18 U.S.C.
2510-2521, is confidential and may be legally privileged.  If you are
not the intended recipient, you are hereby notified that any
retention, dissemination, distribution, or copying of this
communication is strictly prohibited.  Please reply to the sender
that you have received the message in error, then delete it.  Thank
you.

From: Confocal Microscopy List
[[hidden email]] On Behalf Of Stephen C.
Kempf
Sent: Wednesday, March 04, 2009 10:44 AM
Subject: MRC 1024 question

We're having a problem with our BioRad MRC 1024 confocal that I hope
someone else on this list has experienced and resolved. When using
the scope a week or so ago, it stopped producing images and started
giving solid green, red or blue scans on the various channels. A few
days later, one of my other students used it and it was working
properly again. Then this weekend, we got the solid green, red or
blue scans again. We tried various changes in settings, different
accounts, different methods, retarting the software, and restarting
the system, but to no avail. Can any one offer some insight as to
what the problem might be?

Thank you,

Steve Kempf
_____________________________________________________________________
_
________
Stephen C.
Kempf
                             E-mail: [hidden email] Associate
Professor
                             Tel:  334-844-3924
Faculty Director, AU Hybridoma Facility
Cell: 334-703-6689
Department of Biological Sciences
Fax: 334-844-9234
331 Funchess Hall
Auburn University, AL  36849
faculty/webpages/kempf/index.htm
Course web sites:
vert_embryo/index_vert_embryo.html
index_histology.html
Stephen C. Kempf Stephen C. Kempf
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Re: RES: MRC 1024 question

In reply to this post by Renato A. Mortara
Renato,

Thanks for your suggestions. Once I can get to the scope again, I'll give your suggestions a try.

Steve
______________________________________________________________________________
Stephen C. Kempf E-mail: [hidden email]
Associate Professor Tel:  334-844-3924
Faculty Director, AU Hybridoma Facility Cell: 334-703-6689
Department of Biological Sciences Fax: 334-844-9234
331 Funchess Hall
Auburn University, AL  36849
Course web sites:


On Mar 4, 2009, at 1:03 PM, Renato Mortara wrote:

Steve,

This is general reply. Besides the VIS board issue, you may also try to
re-seat all the boards in the computer.

Renato



-----Mensagem original-----
De: Confocal Microscopy List [[hidden email]] Em
nome de George Ring
Enviada em: quarta-feira, 4 de março de 2009 15:52
Assunto: Re: MRC 1024 question

Steve,

First of all, I have no idea what is causing your problem.  You seem to have
adopted the rule of thumb "When in doubt, reboot".  With a different problem
that we've experienced on our MRC1024, we found that rebooting wasn't
sufficient.  In order to "reboot" the system, we have to physically unplug
the control tower.  Even when it is off, it is still receiving power and
doesn't reset by simply switching it off. 

Good luck,

George


George Ring, Ph.D.
Dept. of Cell and Developmental Biology
SUNY Upstate Medical University
750 E. Adams St.
Syracuse NY  13210
Tel. (315) 464-8595
FAX (315) 464-8535





On Wed, Mar 4, 2009 at  1:38 PM, in message
<[hidden email]>, "Stephen C. Kempf"
<[hidden email]> wrote: 
Tom,

I wish it was that simple. The system is running with the scanning 
software (not emulation) and one of my students just told me that it 
happened in the middle of a scan that was progressing normally the 
other day. Seems to me like it might be a hardware problem.

Steve

On Mar 4, 2009, at 10:57 AM, Moninger, Thomas wrote:

Steve,

Well, let*s check the easiest possibility first. Could it be that the 
software is occasionally being started in the *No hardware* or

emulation mode? What you describe is exactly what happens when you 
try to scan in emulation mode.

Tom

Notice: This UI Health Care e-mail (including attachments) is covered 
by the Electronic Communications Privacy Act, 18 U.S.C.
2510-2521, is confidential and may be legally privileged.  If you are 
not the intended recipient, you are hereby notified that any 
retention, dissemination, distribution, or copying of this 
communication is strictly prohibited.  Please reply to the sender 
that you have received the message in error, then delete it.  Thank 
you.

From: Confocal Microscopy List
[[hidden email]] On Behalf Of Stephen C.  
Kempf
Sent: Wednesday, March 04, 2009 10:44 AM
Subject: MRC 1024 question

We're having a problem with our BioRad MRC 1024 confocal that I hope 
someone else on this list has experienced and resolved. When using 
the scope a week or so ago, it stopped producing images and started 
giving solid green, red or blue scans on the various channels. A few 
days later, one of my other students used it and it was working 
properly again. Then this weekend, we got the solid green, red or 
blue scans again. We tried various changes in settings, different 
accounts, different methods, retarting the software, and restarting 
the system, but to no avail. Can any one offer some insight as to 
what the problem might be?

Thank you,

Steve Kempf
_____________________________________________________________________
_
________
Stephen C.  
Kempf                                                                  
                              E-mail: [hidden email] Associate
Professor                                                              
                              Tel:  334-844-3924
Faculty Director, AU Hybridoma Facility                           
Cell: 334-703-6689
Department of Biological Sciences                                  
Fax: 334-844-9234
331 Funchess Hall
Auburn University, AL  36849
faculty/webpages/kempf/index.htm
Course web sites:
vert_embryo/index_vert_embryo.html
index_histology.html
Stephen C. Kempf Stephen C. Kempf
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Re: MRC 1024 question

In reply to this post by George Ring
George,

Thanks for your suggestions. Once I can get to the scope again, I'll keep your comments about re-booting in mind..

Steve
______________________________________________________________________________
Stephen C. Kempf E-mail: [hidden email]
Associate Professor Tel:  334-844-3924
Faculty Director, AU Hybridoma Facility Cell: 334-703-6689
Department of Biological Sciences Fax: 334-844-9234
331 Funchess Hall
Auburn University, AL  36849
Course web sites:


On Mar 4, 2009, at 12:51 PM, George Ring wrote:

Steve,

First of all, I have no idea what is causing your problem.  You seem to
have adopted the rule of thumb "When in doubt, reboot".  With a different
problem that we've experienced on our MRC1024, we found that rebooting
wasn't sufficient.  In order to "reboot" the system, we have to physically
unplug the control tower.  Even when it is off, it is still receiving power
and doesn't reset by simply switching it off. 

Good luck,

George


George Ring, Ph.D.
Dept. of Cell and Developmental Biology
SUNY Upstate Medical University
750 E. Adams St.
Syracuse NY  13210
Tel. (315) 464-8595
FAX (315) 464-8535





On Wed, Mar 4, 2009 at  1:38 PM, in message
<[hidden email]>, "Stephen C. Kempf"
<[hidden email]> wrote: 
Tom,

I wish it was that simple. The system is running with the scanning  
software (not emulation) and one of my students just told me that it  
happened in the middle of a scan that was progressing normally the  
other day. Seems to me like it might be a hardware problem.

Steve

On Mar 4, 2009, at 10:57 AM, Moninger, Thomas wrote:

Steve,

Well, let*s check the easiest possibility first. Could it be that  
the software is occasionally being started in the *No hardware* or 

emulation mode? What you describe is exactly what happens when you  
try to scan in emulation mode.

Tom

Notice: This UI Health Care e-mail (including attachments) is  
covered by the Electronic Communications Privacy Act, 18 U.S.C.  
2510-2521, is confidential and may be legally privileged.  If you  
are not the intended recipient, you are hereby notified that any  
retention, dissemination, distribution, or copying of this  
communication is strictly prohibited.  Please reply to the sender  
that you have received the message in error, then delete it.  Thank  
you.

From: Confocal Microscopy List  
[[hidden email]] On Behalf Of Stephen C.  
Kempf
Sent: Wednesday, March 04, 2009 10:44 AM
Subject: MRC 1024 question

We're having a problem with our BioRad MRC 1024 confocal that I  
hope someone else on this list has experienced and resolved. When  
using the scope a week or so ago, it stopped producing images and  
started giving solid green, red or blue scans on the various  
channels. A few days later, one of my other students used it and it  
was working properly again. Then this weekend, we got the solid  
green, red or blue scans again. We tried various changes in  
settings, different accounts, different methods, retarting the  
software, and restarting the system, but to no avail. Can any one  
offer some insight as to what the problem might be?

Thank you,

Steve Kempf
______________________________________________________________________ 
________
Stephen C.  
Kempf                                                                  
                              E-mail: [hidden email]
Associate  
Professor                                                              
                              Tel:  334-844-3924
Faculty Director, AU Hybridoma Facility                           
Cell: 334-703-6689
Department of Biological Sciences                                  
Fax: 334-844-9234
331 Funchess Hall
Auburn University, AL  36849
faculty/webpages/kempf/index.htm
Course web sites:
vert_embryo/index_vert_embryo.html
index_histology.html
Stephen C. Kempf Stephen C. Kempf
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Re: MRC 1024 question

In reply to this post by Nuno Moreno
Nuno,

Thanks for your suggestions. Once I can get to the scope again, I'll keep the possible pre-amp problem in mind.

Steve
______________________________________________________________________________
Stephen C. Kempf E-mail: [hidden email]
Associate Professor Tel:  334-844-3924
Faculty Director, AU Hybridoma Facility Cell: 334-703-6689
Department of Biological Sciences Fax: 334-844-9234
331 Funchess Hall
Auburn University, AL  36849
Course web sites:

On Mar 4, 2009, at 10:46 AM, Nuno Moreno wrote:

Welcome to the world of problems with pre-amps. The similar problems I had up to know are related with it.


Regards,
NM



Stephen C. Kempf wrote:
We're having a problem with our BioRad MRC 1024 confocal that I hope someone else on this list has experienced and resolved. When using the scope a week or so ago, it stopped producing images and started giving solid green, red or blue scans on the various channels. A few days later, one of my other students used it and it was working properly again. Then this weekend, we got the solid green, red or blue scans again. We tried various changes in settings, different accounts, different methods, retarting the software, and restarting the system, but to no avail. Can any one offer some insight as to what the problem might be?
Thank you,
Steve Kempf
______________________________________________________________________________
Stephen C. Kempf E-mail: [hidden email] <[hidden email]>
Associate Professor Tel:  334-844-3924
Faculty Director, AU Hybridoma Facility Cell: 334-703-6689
Department of Biological Sciences Fax: 334-844-9234
331 Funchess Hall
Auburn University, AL  36849
*Course web sites:*
*   Histology*  - http://www.auburn.edu/academic/classes/zy/hist0509/index_histology.html <http://www.auburn.edu/academic/classes/zy/hist0509/index_histologyhtml>
Catherine GARNIER Catherine GARNIER
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Re: components of Zeiss LSM 410

In reply to this post by Catherine GARNIER
    Hello,

    One thing to add to clarify the first message. The microscope associated to the LSM 410 has been transformed in classical fluorescence microscope.
     As we will have very soon a new confocal system, we sell/give other components of our old system.
        3 lasers, changed in June 2004,  used around 1500 h:
            - Laser Argon référence Zeiss 000000 0234 267
            - Laser He Ne 543 nm référence Zeiss 577009 0778 400
            - Laser He Ne 633 nm référence Zeiss 577009 0709 100

        2 PMTs,  changed in June 2006 :
            - PMT  N°R4632 (Hamamatsu)
            - PMT  N°R3896 (Hamamatsu)

         anti-vibration  table 193 x 123 x 88 cm (L x l x h)

and any other electronic components....

Catherine

[hidden email] 
-- 
**************************************************************************
Dr Catherine Garnier
Institut National de la Recherche Agronomique - INRA
Biopolymères, Interactions, Assemblages - BIA
Interfaces et Systèmes Dispersés - ISD
Rue de la Géraudière - BP 71627 - 44316 Nantes cedex 3
Tel : 33 (0)2 40 67 50 45
Fax: 33 (0)2 40 67 50 84
http://www.angers-nantes.inra.fr
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Dr Catherine Garnier
Institut National de la Recherche Agronomique - INRA
Biopolymères, Interactions, Assemblages - BIA
Interfaces et Systèmes Dispersés - ISD
Rue de la Géraudière - BP 71627 - 44316 Nantes cedex 3
Tel : 33 (0)2 40 67 50 45
Fax: 33 (0)2 40 67 50 84
http://www.nantes.inra.fr
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Poirier, Rick Poirier, Rick
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Re: Axiovert 200M question

In reply to this post by Arvydas Matiukas

Hi Arvydas,

We’ve had similar problems with our 510 system and we resolved it by closing the program and shutting down the E-box for 15sec then restarting. The problem usually occurred when users switched quickly and often between VIS and LSM mode, but on occasion when restarting the E-box did not reset it we would shut down the entire system and that usually resolved the problem. This does not solve the problem however, it merely treated the symptoms. Having the tech come and blow out the E-box and reseat some of the boards has by and large fixed the problem. The problem can also arise when a component in the system times out and the controller gets confused. This again can be resolved by restarting the e-box, microscope or entire system. In the case of a time out you may wish to have the tech ensure all the hardware within the microscope is moving freely and there is enough delay built into the system for it to home itself.

Rick

From: Confocal Microscopy List [mailto:[hidden email]] On Behalf Of Arvydas Matiukas
Sent: March 4, 2009 3:06 PM
To: [hidden email]
Subject: Axiovert 200M question

 

Hi all,

 

We are having a problem with our Zeiss Axiovert 200M. It stopped

responding to any buttons. Fuses are OK, and it is getting power.

On the back red LED is on (as well as green one), and LCD screen

is showing squares. Can anyone  offer some insight as to what the

problem might be? LSM software is showing error messages for

COM1 but computer is OK so I assume the problem is on the scope

side.

Thank you,

Arvydas

 

 

Arvydas Matiukas, Ph.D.
Director of Confocal&Two-Photon Imaging Core Facility
Department of Pharmacology
SUNY Upstate Medical University
766 Irving Ave., WH 3159
Syracuse, NY 13210
tel.: 315-464-7997
fax: 315-464-8014
email: [hidden email]
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