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Hi Daniel,
I'm glad to see that 600s are still out there and being used. The
MRC600 is really a pretty amazing machine - we have one that's been
basically turned on since 1992 and still runs perfectly (it's mounted up
on an upright system and it's hard to reach the on/off switch so everyone
leaves it on).
I assume you mean switches back between channels (768 x 512). This one
takes z stacks, but if you just want at single image (from each channel)
at each time point either set %2 to 1 or remove the second for loop
altogether.
For z-stepping you'll need to change the $si %3 to the MPL
motor command (i.e. motor %3). We use a different z stepper and
talk to it by the com port and a small executable (si.exe) that MPL
shells out to.
Save the macro as a text file to something like z2ch.cmd.
to use - as an example, to take a 10 image stack over 10 time points at a
2 micron step and a 10 sec wait type:
%z2ch a 10 2 3 10 10
You end up with 20 z-stacks named ach1_1.pic, ach2_1.pic etc...
! 2 channel zseries over time
!%1=filename %2 = # of sections %3 = stepsize
!%4 = # of aves %5 = wait (in secs) %6 = #stacks
wait 0
live filter k %4
for t = 1, %6
for i = 1, %2
print Image #i of %2, channel 1
live ch 1
collect
put %1c1_#t #i
print Image #i of %2, channel 2
live ch 2
collect
put %1c2_#t #i
$si %3
wait %5
next i
next t
live filter k 0
live filter direct
At 12:57 PM 1/18/2008, you wrote:
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Hi,
Are there any retro MRC-600 users who can help me please?
Here in Liverpool, we are happily still using both an upright and an
inverted MRC-600.
I would like to use the inverted system to take time lapse images of live
cells and collect images from both channels. The software allow me to do
this, but only to collect a half screen image from each channel. Does
anyone have a self penned cmd file that allows collection of 2 full
screen images over time?
Thanks,
Daniel
Daniel Brotchie
Ophthalmology
University of Liverpool
UK
[hidden email]
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