MaiTai DeepSee adjustments on multiphoton microscope

[Richard Cole]

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Hi Michael,

I have the MaiTai on a Leica SP5 and experience ~ 4X brightness increase
and can image ~10-15% deeper (very sample dependent) with the DeepSee.  I
have found that adjusting the motor position makes a significant
difference over the lookup table values.  For us, well worth the trouble.

Cheers

Rich

Richard Cole
Research Scientist V
Director: Advanced Light Microscopy & Image Analysis Core
Wadsworth Center
P.O. Box 509 Albany N.Y. 12201-0509

Research Assistant Professor
Dept. of Biomedical Sciences
School of Public Health State University of New York Albany, New York

518-474-7048
 518-474-4430
 [hidden email]
Website www.wadsworth.org/cores/alm/index.htm


Hi Michael,

We have the DeepSee on our MaiTai and have found that it doesn't do a lot
on our system. At extreme depths, say greater than 500um you can get a
small enhancement in signal strength and quality but it is only very
minor. It may give you a few extra microns penetration but thats about it.

It really depends on how much the optics of your system are
dispersing/chirping the beam. If they are not altering it much then
pre-chirping it will not do a lot.

This is all based on qualitative assessment, i have not quantitated any of
this. Also we have recently received a new Olympus 25x NA1.05 objective
specifically designed for MP imaging. I have not seen how this performs
yet with the DeepSee.


Cheers

Cam



Cameron J. Nowell
Microscopy Manager
Centre for Advanced Microscopy
Ludwig Institute for Cancer Research
PO Box 2008
Royal Melbourne Hospital
Victoria, 3050
AUSTRALIA
Office: [phone]
Mobile: [phone]
Fax: [phone]
Facility Website



-----Original Message-----
From: Confocal Microscopy List [mailto:[hidden email]]
On Behalf Of Cammer, Michael
Sent: Friday, 19 November 2010 4:08 AM
To: [hidden email]
Subject: MaiTai DeepSee adjustments on multiphoton microscope

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Dear microscopists,

I'm planning to go through a regimen of  adjusting  the MaiTai DeepSee on
a variety of samples with different optics  to see what changes there are
in depth penetration (and, maybe, preservation of biological activity).

Does anybody have experience with this?  If so, have you found that the
DeepSee provides a noticeable practical benefit over the MaiTai without
it?

Any comments welcome and please feel free to contact me offline at
[hidden email]

Thank you!

Sincerely,

Michael C.







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[mcammer]

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Dear Rich,

Thanks for the response.  Sounds like you're up and running since we last saw each other a few years ago.  Yes, I'm back, although not running a facility.  I missed microscopy too much and had to come back!

Hope you're well.

Sincerely,

Michael

_________________________________________
Michael Cammer, Assistant Research Scientist
Skirball Institute of Biomolecular Medicine
Lab: [phone]  Cell: [phone]

________________________________________
From: Confocal Microscopy List [[hidden email]] On Behalf Of Richard Cole [[hidden email]]
Sent: Friday, November 19, 2010 8:22 PM
To: [hidden email]
Subject: MaiTai DeepSee adjustments on multiphoton microscope

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Hi Michael,

I have the MaiTai on a Leica SP5 and experience ~ 4X brightness increase
and can image ~10-15% deeper (very sample dependent) with the DeepSee.  I
have found that adjusting the motor position makes a significant
difference over the lookup table values.  For us, well worth the trouble.

Cheers

Rich

Richard Cole
Research Scientist V
Director: Advanced Light Microscopy & Image Analysis Core
Wadsworth Center
P.O. Box 509 Albany N.Y. 12201-0509

Research Assistant Professor
Dept. of Biomedical Sciences
School of Public Health State University of New York Albany, New York


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