Mattek dishes
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Hi List,
We use Bellco Sykes-Moore chambers. http://www.bellcoglass.com/products.aspx?category_id=11&sub_id=109&sub_id_2=361 No commercial interest and thanks to Stephen Cody for getting these in. Cheers Cam Cameron J. Nowell Microscopy Manager Centre for Advanced Microscopy Ludwig Institute for Cancer Research PO Box 2008 Royal Melbourne Hospital Victoria, 3050 AUSTRALIA Office: +61 3 9341 3155 Mobile: +61422882700 Fax: +61 3 9341 3104 Facility Website -----Original Message----- From: Confocal Microscopy List [mailto:[hidden email]] On Behalf Of Craig Brideau Sent: Friday, 6 November 2009 8:33 AM To: [hidden email] Subject: Re: Mattek dishes I agree. Trying to make these things is time consuming. Craig On Thu, Nov 5, 2009 at 2:26 PM, Kristi DeCourcy <[hidden email]> wrote: > Actually, if the user is that...frugal, we used to make our own dishes. All > it takes is petris, a small drill press, glass coverslips, and glue. The > only thing to be careful about it that you smooth down the drilled edges of > the petri, so that the coverslip is flat against the dish bottom when you > glue it. > And I still remember my joyous reaction to the discovery that I could buy > the same thing from Mattek! > Kristi > > Has anyone ever reused Mattek dishes? I have a user who is trying to save > some money and is cleaning their dishes with a detergent, rinsing and > exposing to UV light. The cells look "funny' to me in that I can't seem to > get a crisp image. Does anyone have any experience with reusing dishes? > > > > Thx! > > > > Mary > > -- > > ************************************ > > Kristi R. DeCourcy, Ph.D. > Research Associate/Laboratory Manager > Fralin Life Science Institute > Virginia Tech > West Campus Drive > Blacksburg, VA 24061 > Phone: (540) 231-7959 > Cell (540) 392-9814 > Fax: (540) 231-7126 > http://www.biotech.vt.edu No virus found in this incoming message. Checked by AVG - www.avg.com Version: 8.5.424 / Virus Database: 270.14.49/2480 - Release Date: 11/04/09 07:37:00 This communication is intended only for the named recipient and may contain information that is confidential, legally privileged or subject to copyright; the Ludwig Institute for Cancer Research Ltd does not waive any rights if you have received this communication in error. The views expressed in this communication are those of the sender and do not necessarily reflect the views of the Ludwig Institute for Cancer Research Ltd. |
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In reply to this post by Jennes, Mary E
I have two suggestions. We use dishes from World Precision Instruments that
are more affordable. I was in a lab that made their own and I actually worked it out. By the time you bought the supplies and paid a student to make the dishes it is actually about the same price to buy the dishes. We even had a problem with some of the WPI dishes having debris on them that we could see in TIRF microscopy. They fixed the problem and gave us new dishes for all the ones we had ordered.The dishes also have a nice large viewing area. It works out to about $2 per dish. http://www.wpiinc.com/index.php?option=com_virtuemart&page=shop.browse&category_id=212&Itemid=127 Secondly we use a similar chamber for standard coverslips but it is magnetic so I find you don't end up with all the problem of leaking with the screw based systems. It is sold by a company called Live Cell which is distributed by Quorum Technologies in Canada. http://www.quorumtechnologies.com/Environment_Systems.html No commercial interest just a happy customer. Claire |
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In reply to this post by Vladimir Ghukasyan-2
Dear Vladimir,
I am interested in getting more information about the metallic rings. Thanks. El 05/11/2009 21:32, Vladimir Gukassyan escribió: > Instead of Mattek dishes we're just using a system of metallic rings > and 24mm round coverslips. This is much cheaper solution. Let me know > if you're interested, I'll send you a photo of the rings we have. > > With regards, > Vladimir > > ----------------------------------------- > Vladimir Ghukasyan, Ph.D. > Institute of Biophotonics > National Yang-Ming University > 155, Li-Nong St., Sec. 2 > Taipei 112, Taiwan > Tel.: (886 2) 28267000 ext. 5709 > > > On Fri, Nov 6, 2009 at 1:46 AM, Jennes, Mary E<[hidden email]> wrote: > >> Has anyone ever reused Mattek dishes? I have a user who is trying to save >> some money and is cleaning their dishes with a detergent, rinsing and >> exposing to UV light. The cells look “funny’ to me in that I can’t seem to >> get a crisp image. Does anyone have any experience with reusing dishes? >> >> >> >> Thx! >> >> >> >> Mary >> > F Javier Díez Guerra, PhD Profesor Titular Centro de Biología Molecular Severo Ochoa C/ Nicolás Cabrera, 1 Universidad Autónoma Ctra Colmenar Viejo Km 15 Cantoblanco, 28049 Madrid SPAIN phone: +34 91 196 4612 e-mail: [hidden email] |
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regards F Javier Díez Guerra wrote: Dear Vladimir, --
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In reply to this post by Claire Brown
Dear All,
We have a SpectraPhysics BeamLok 2060 (water cooled) ArKr laser (total power approx. 2.5 W) to give away. The laser would need a tube refurbishment - so it is not functional right now. Nevertheless, until the tube problem it was working fine (we used it for a spinning disc microscope) and the SpectraPhysics service confirmed that it is only the tube what is defect. If you want it - your should organize the shipping and other administrative issues (and obviously cover those costs). Interested people should contact me directly. Greetings Gabor -- Gabor Csucs Light Microscopy Centre, ETH Zurich Schafmattstrasse 18, HPM F16 CH-8093, Zurich, Switzerland Web: www.lmc.ethz.ch Phone: +41 44 633 6221 Mobile: +41 79 758 21 58 Fax: +41 44 632 1298 e-mail: [hidden email] |
 
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Romin, Yevgeniy/Sloan Kettering Institute |
Re: Mattek dishes
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In reply to this post by Vladimir Ghukasyan-2
Dear Vladimir
I would also like to get more info on the metallic rings -----Original Message----- From: Confocal Microscopy List [mailto:[hidden email]] On Behalf Of Vladimir Gukassyan Sent: Thursday, November 05, 2009 3:33 PM To: [hidden email] Subject: Re: Mattek dishes Instead of Mattek dishes we're just using a system of metallic rings and 24mm round coverslips. This is much cheaper solution. Let me know if you're interested, I'll send you a photo of the rings we have. With regards, Vladimir ----------------------------------------- Vladimir Ghukasyan, Ph.D. Institute of Biophotonics National Yang-Ming University 155, Li-Nong St., Sec. 2 Taipei 112, Taiwan Tel.: (886 2) 28267000 ext. 5709 On Fri, Nov 6, 2009 at 1:46 AM, Jennes, Mary E <[hidden email]> wrote: > Has anyone ever reused Mattek dishes? I have a user who is trying to save > some money and is cleaning their dishes with a detergent, rinsing and > exposing to UV light. The cells look "funny' to me in that I can't seem to > get a crisp image. Does anyone have any experience with reusing dishes? > > > > Thx! > > > > Mary ===================================================================== Please note that this e-mail and any files transmitted with it may be privileged, confidential, and protected from disclosure under applicable law. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any reading, dissemination, distribution, copying, or other use of this communication or any of its attachments is strictly prohibited. If you have received this communication in error, please notify the sender immediately by replying to this message and deleting this message, any attachments, and all copies and backups from your computer. |
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In reply to this post by Stefano Giovannardi
Dear Stefano,
Could you share your observations on the bad influence from metal? Which metal did you use? Thank you in advance, Vladimir On Fri, Nov 6, 2009 at 10:10 PM, Stefano Giovannardi <[hidden email]> wrote:
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In reply to this post by Cameron Nowell
Following on from the discussion on cleaning cover-slips a while ago, any tissue digest such as acids/bleach should help remove cellular material from the dishes, followed by standard detergent then alcohol washes. However sterilization is a bit of a pain, as autoclaving will deform the plastic - due to the plastic they are safe only up to 50oC. Probably uV is the only way to go, unless you happen to have a massive gamma irradiation facility nearby [which actually we do, at Harwell]. Back at UCL we did try fabricating our own Mattek dishes from plastic Petri dishes but our workshop had trouble cutting the hole without splintering the plastic a little - I expect Mattek dishes holes are laser cut [which is fast and works brilliantly and you could even have a unique number/ident/logo etched into the plastic as well]. It really wasn't cost effective to make them in-house, particularly as our workshop starting charging an hourly rate.
Around 25 years ago we used to use glass 'tubes' around a few centimeters tall, machine cut from a glass tube ~3.5cm diameter [that took a standard plastic or glass Petri dish lid, but I think we machined our own re-usable lid]. The bottom rim was ground flat and we [well our workshop chaps] glued a very thin plastic membrane to the bottom - probably using a silicon type adhesive as Mattek do, but I'd have to check that. This membrane was only a few um thick from memory, probably 20 to 50um - it was ICI Melinex and available in a range of Thicknesses]. The wells were then sterilized within one of our massive walk in gamma irradiation facilities [originally constructed to irradiate large animals, e.g. goats, as you did back in the 1950/60s]. The cells grew well on this membrane and were imaged under our MRC 500 and latterly 1024 confocal [c1980/90s]. After use the membrane was taken off, the glass washed in a glass washing machine, and a new membrane fitted and the culture vessels then irradiated on-mass. All very well and good, but now I use pre-sterilized Mattek dishes instead as being only £1 each it's a lot less trouble, particularly now I don't have free access to the gamma cells or an in-house workshop. I do regularly re-use Mattek* dishes, but also to produce microscopy test samples using things like fluorescent spheres, salt crystals doped with fluorescence, micro-fossils and the like - never to re-culture cells. We tend to club together with other groups to buy many hundreds of Mattek Petri dishes at a time and save on postage costs [quite high to the UK]. At most I only use a few a day so it's not many pennies in the scheme of things [even when using the rather more expensive Mattek multiwell plates]. Regards Keith There are other suppliers of similar 'confocal dishes', e.g. PAA Labs: http://www.paa.com/products/plastic_labware/cell_culture/confocaldishescoverglass_bott.html But they are of comparable price. For more cell culture/microscope imaging products links see our web site: http://www.well.ox.ac.uk/external-website-links and see: Microscopy Live Cell Imaging and Anti-Fadent Mountants If you know of any other similar products I'll be pleased to add them --------------------------------------------------------------------------- Dr Keith J. Morris, Molecular Cytogenetics and Microscopy Core, Laboratory 00/069 and 00/070, The Wellcome Trust Centre for Human Genetics, Roosevelt Drive, Oxford OX3 7BN, United Kingdom. Telephone: +44 (0)1865 287568 Email: [hidden email] Web-pages: http://www.well.ox.ac.uk/molecular-cytogenetics-and-microscopy -----Original Message----- From: Confocal Microscopy List [mailto:[hidden email]] On Behalf Of Cameron Nowell Sent: 05 November 2009 21:35 To: [hidden email] Subject: Re: Mattek dishes Hi List, We use Bellco Sykes-Moore chambers. http://www.bellcoglass.com/products.aspx?category_id=11&sub_id=109&sub_id_2=361 No commercial interest and thanks to Stephen Cody for getting these in. Cheers Cam Cameron J. Nowell Microscopy Manager Centre for Advanced Microscopy Ludwig Institute for Cancer Research PO Box 2008 Royal Melbourne Hospital Victoria, 3050 AUSTRALIA Office: +61 3 9341 3155 Mobile: +61422882700 Fax: +61 3 9341 3104 Facility Website -----Original Message----- From: Confocal Microscopy List [mailto:[hidden email]] On Behalf Of Craig Brideau Sent: Friday, 6 November 2009 8:33 AM To: [hidden email] Subject: Re: Mattek dishes I agree. Trying to make these things is time consuming. Craig On Thu, Nov 5, 2009 at 2:26 PM, Kristi DeCourcy <[hidden email]> wrote: > Actually, if the user is that...frugal, we used to make our own dishes. All > it takes is petris, a small drill press, glass coverslips, and glue. The > only thing to be careful about it that you smooth down the drilled edges of > the petri, so that the coverslip is flat against the dish bottom when you > glue it. > And I still remember my joyous reaction to the discovery that I could buy > the same thing from Mattek! > Kristi > > Has anyone ever reused Mattek dishes? I have a user who is trying to save > some money and is cleaning their dishes with a detergent, rinsing and > exposing to UV light. The cells look "funny' to me in that I can't seem to > get a crisp image. Does anyone have any experience with reusing dishes? > > > > Thx! > > > > Mary > > -- > > ************************************ > > Kristi R. DeCourcy, Ph.D. > Research Associate/Laboratory Manager > Fralin Life Science Institute > Virginia Tech > West Campus Drive > Blacksburg, VA 24061 > Phone: (540) 231-7959 > Cell (540) 392-9814 > Fax: (540) 231-7126 > http://www.biotech.vt.edu No virus found in this incoming message. Checked by AVG - www.avg.com Version: 8.5.424 / Virus Database: 270.14.49/2480 - Release Date: 11/04/09 07:37:00 This communication is intended only for the named recipient and may contain information that is confidential, legally privileged or subject to copyright; the Ludwig Institute for Cancer Research Ltd does not waive any rights if you have received this communication in error. The views expressed in this communication are those of the sender and do not necessarily reflect the views of the Ludwig Institute for Cancer Research Ltd. |
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Hello,
Can somebody point to a vendor beside Invitrogen for the FITC dextran of 3000 M.W. I am trying to do some permeability studies. Thanks, -Prabhakar |
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