Measurements in Imaris

[Dun, Alison]

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Hi

Have a facility user who is trying to make measurements using Imaris (version 7.3.1) and having some difficulties. Ideally she would like to use the slice section but cannot see how to do this in the software - maybe it is an upgrade we don’t have? Is there a way to do it in 3D consistently? When you move the navigator tool the line is not where you attempted to put it! Is there another software that is better to use?

Any help would be much appreciated!
Ali

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Heriot-Watt University is The Times & The Sunday Times International University of the Year 2018

Founded in 1821, Heriot-Watt is a leader in ideas and solutions. With campuses and students across the entire globe we span the world, delivering innovation and educational excellence in business, engineering, design and the physical, social and life sciences.

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[Ian Dobbie]

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Hi Ali

I’m not sure this is the same issue but we found that many functions in imaris move things to the nearest pixel so basically don’t work at sub pixel resolution.  Totally invalidates any particle tracking in imaris!

Ian

Sent from my iPhone

> On 14 Mar 2018, at 14:24, Dun, Alison <[hidden email]> wrote:
>
> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> Post images on http://www.imgur.com and include the link in your posting.
> *****
>
> Hi
>
> Have a facility user who is trying to make measurements using Imaris (version 7.3.1) and having some difficulties. Ideally she would like to use the slice section but cannot see how to do this in the software - maybe it is an upgrade we don’t have? Is there a way to do it in 3D consistently? When you move the navigator tool the line is not where you attempted to put it! Is there another software that is better to use?
>
> Any help would be much appreciated!
> Ali
>
> ________________________________
>
> Heriot-Watt University is The Times & The Sunday Times International University of the Year 2018
>
> Founded in 1821, Heriot-Watt is a leader in ideas and solutions. With campuses and students across the entire globe we span the world, delivering innovation and educational excellence in business, engineering, design and the physical, social and life sciences.
>
> This email is generated from the Heriot-Watt University Group, which includes:
>
>  1.  Heriot-Watt University, a Scottish charity registered under number SC000278
>  2.  Edinburgh Business School a Charity Registered in Scotland, SC026900. Edinburgh Business School is a company limited by guarantee, registered in Scotland with registered number SC173556 and registered office at Heriot-Watt University Finance Office, Riccarton, Currie, Midlothian, EH14 4AS
>  3.  Heriot- Watt Services Limited (Oriam), Scotland's national performance centre for sport. Heriot-Watt Services Limited is a private limited company registered is Scotland with registered number SC271030 and registered office at Research & Enterprise Services Heriot-Watt University, Riccarton, Edinburgh, EH14 4AS.
>
> The contents (including any attachments) are confidential. If you are not the intended recipient of this e-mail, any disclosure, copying, distribution or use of its contents is strictly prohibited, and you should please notify the sender immediately and then delete it (including any attachments) from your system.

[PRICE Meredith]

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Dear Alison and Ian,

Thank you for using Imaris and I hope my reply below is helpful.

Alison, based on your description it sounds like the user needs to make a 3D line measurement.  This type of measurement is possible with our MeasurementPoints tool which can be used with our Ortho and Oblique Slicers.  You will find a short video tutorial covering this topic here: http://www.bitplane.com/learning/measurement-points-and-usage-of-ortho-and-oblique-slicers-video-tutorial   And please feel free to reach out to me directly if this doesn't solve the issue.

I also want to assure Ian and the entire community that spot detection in Imaris, including MeasurementPoints and Spots, places the center with subpixel precision.  When using automatic detection during the Spots wizard the precision depends upon the width of the applied filter (i.e. estimated XY diameter).

A possible place for confusion could be that the display of measurement values within Imaris is controlled by a preference for the number of visible significant digits.  This setting is adjustable and does not affect the export of these values to Excel, etc..

I'd like to ensure that we fully address your concerns so please reach out to our Support team to review images and analysis results where you've seen this spot placement behavior.

Thanks again,
Meredith

Meredith Price
Imaris Product Manager

Mobile  [phone]
Skype   mhprice79
Web     bitplane.com


-----Original Message-----
From: Confocal Microscopy List [mailto:[hidden email]] On Behalf Of Ian Dobbie
Sent: Wednesday, March 14, 2018 10:33 AM
To: [hidden email]
Subject: -|EXT|- Re: Measurements in Imaris

*****
To join, leave or search the confocal microscopy listserv, go to:
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
Post images on http://www.imgur.com and include the link in your posting.
*****

Hi Ali

I'm not sure this is the same issue but we found that many functions in imaris move things to the nearest pixel so basically don't work at sub pixel resolution.  Totally invalidates any particle tracking in imaris!

Ian

Sent from my iPhone

> On 14 Mar 2018, at 14:24, Dun, Alison <[hidden email]> wrote:
>
> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> Post images on http://www.imgur.com and include the link in your posting.
> *****
>
> Hi
>
> Have a facility user who is trying to make measurements using Imaris (version 7.3.1) and having some difficulties. Ideally she would like to use the slice section but cannot see how to do this in the software - maybe it is an upgrade we don't have? Is there a way to do it in 3D consistently? When you move the navigator tool the line is not where you attempted to put it! Is there another software that is better to use?
>
> Any help would be much appreciated!
> Ali
>
> ________________________________
>
> Heriot-Watt University is The Times & The Sunday Times International
> University of the Year 2018
>
> Founded in 1821, Heriot-Watt is a leader in ideas and solutions. With campuses and students across the entire globe we span the world, delivering innovation and educational excellence in business, engineering, design and the physical, social and life sciences.
>
> This email is generated from the Heriot-Watt University Group, which includes:
>
>  1.  Heriot-Watt University, a Scottish charity registered under
> number SC000278  2.  Edinburgh Business School a Charity Registered in
> Scotland, SC026900. Edinburgh Business School is a company limited by guarantee, registered in Scotland with registered number SC173556 and registered office at Heriot-Watt University Finance Office, Riccarton, Currie, Midlothian, EH14 4AS  3.  Heriot- Watt Services Limited (Oriam), Scotland's national performance centre for sport. Heriot-Watt Services Limited is a private limited company registered is Scotland with registered number SC271030 and registered office at Research & Enterprise Services Heriot-Watt University, Riccarton, Edinburgh, EH14 4AS.
>
> The contents (including any attachments) are confidential. If you are not the intended recipient of this e-mail, any disclosure, copying, distribution or use of its contents is strictly prohibited, and you should please notify the sender immediately and then delete it (including any attachments) from your system.

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