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Hi Etai,
We have recently found that gold nanoparticles work well for PSF
measurement in solvents. The scattering from them is extremely bright,
they do not photobleach, and a single nanoparticle can be imaged at various
wavelengths.
On Mon, Jun 4, 2018 at 11:32 AM, Etai Sapoznik <
[hidden email]> wrote:
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> To join, leave or search the confocal microscopy listserv, go to:
>
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy> Post images on
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> *****
>
> Dear all,
> We are interested in assessing the PSF of our microscope when imaging in
> Benzyl Alcohol/ Benzyl Benzoate (BABB) solution (used for tissue clearing).
> What do you use as a point source when the clearing medium involves such
> organic solvents?
>
> In our hands, Polystyrene beads seem to be dissolved in BABB while Quantum
> dots have issues with aggregation.
>
> Thank you in advance for any advice you may have,
>
> Etai
>
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