*****
To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy ***** Dear all, If any one of you had already compared MetaMorph (we have 7.6 version) with the NIKON Elements software I would appreciate it very much if you share with me your opinion. If you used both of them, how do they compare? Which one would be your personal choice and why? Thank you ahead of time Michelle Aloni MS, HTL (ASCP) Research Specialist USC Keck School of Medicine |
Cameron Nowell |
*****
To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy ***** Hi Michelle Do you want to use them for anlaysis of driving hardware? We have both but use them for different things. NIS AR is used primarily for image capture on a couple of our systems. The EDF (Extended depth of focus) is really useful for some of our imaging. We do not use NIS for image processing (other than EDF) or analysis. NIS has all the usual tools for image processing and analysis but the automation and scripting can be a bit tricky to get your head around. We use MetaMorph for capture as well (automated tile scanning and live cell imaging). For all our analysis we use a combination of MetaMorph, Fiji and Imaris. The bulk of the work is usually done in MetaMorph because its ease of scripting/automation. Part of that ease comes from what you are familiar with of course, but I find MetaMorph's journal editor to be easier to use (with better available tutorials etc) than NIS. Cost could be an issue as MetaMorph is going to be double the price of NIS (for analysis). A metamorph capture system will be more than double NIS. If you are interested in some tutorials etc for MetaMorph I am happy to send you a link to download a heap of training notes (330 page manual) and demo images I have put together over the last two years for a MetaMorph training course I co-ran here in Melbourne. The usual statements apply - no commercial interests in any of the platforms just a happy (sometimes frustrated) user. Cheers Cam Cameron J. Nowell Microscopy Manager Centre for Advanced Microscopy Ludwig Institute for Cancer Research Melbourne - Parkville Branch PO Box 2008 Royal Melbourne Hospital Victoria, 3050 AUSTRALIA Office: +61 3 9341 3158 Mobile: +61 422882700 Fax: +61 3 9341 3104 Facility Website Linked In Profile -----Original Message----- From: Confocal Microscopy List [mailto:[hidden email]] On Behalf Of Michelle Aloni Sent: Tuesday, 13 March 2012 11:29 AM To: [hidden email] Subject: MetaMorph versus Elements? ***** To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy ***** Dear all, If any one of you had already compared MetaMorph (we have 7.6 version) with the NIKON Elements software I would appreciate it very much if you share with me your opinion. If you used both of them, how do they compare? Which one would be your personal choice and why? Thank you ahead of time Michelle Aloni MS, HTL (ASCP) Research Specialist USC Keck School of Medicine |
leoncio vergara |
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To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy ***** I second the opinion of Cameron, his description is almost exactly our experience. I find the automation tools in metamorph a very strong point, I almost don't do any analysis with NIS, however I find NIS better than metamorph for examining multidimensional datasets, it is very intuitive. We also use metamorph (and NIS) in combination with Image J and Imaris, I don't think there is a single package that does everything. Leoncio Vergara Assistant Professor Center for Biomedical Engineering University of Texas Medical Branch Galveston, Texas -----Original Message----- From: Confocal Microscopy List [mailto:[hidden email]] On Behalf Of Cameron Nowell Sent: Monday, March 12, 2012 8:56 PM To: [hidden email] Subject: Re: MetaMorph versus Elements? ***** To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy ***** Hi Michelle Do you want to use them for anlaysis of driving hardware? We have both but use them for different things. NIS AR is used primarily for image capture on a couple of our systems. The EDF (Extended depth of focus) is really useful for some of our imaging. We do not use NIS for image processing (other than EDF) or analysis. NIS has all the usual tools for image processing and analysis but the automation and scripting can be a bit tricky to get your head around. We use MetaMorph for capture as well (automated tile scanning and live cell imaging). For all our analysis we use a combination of MetaMorph, Fiji and Imaris. The bulk of the work is usually done in MetaMorph because its ease of scripting/automation. Part of that ease comes from what you are familiar with of course, but I find MetaMorph's journal editor to be easier to use (with better available tutorials etc) than NIS. Cost could be an issue as MetaMorph is going to be double the price of NIS (for analysis). A metamorph capture system will be more than double NIS. If you are interested in some tutorials etc for MetaMorph I am happy to send you a link to download a heap of training notes (330 page manual) and demo images I have put together over the last two years for a MetaMorph training course I co-ran here in Melbourne. The usual statements apply - no commercial interests in any of the platforms just a happy (sometimes frustrated) user. Cheers Cam Cameron J. Nowell Microscopy Manager Centre for Advanced Microscopy Ludwig Institute for Cancer Research Melbourne - Parkville Branch PO Box 2008 Royal Melbourne Hospital Victoria, 3050 AUSTRALIA Office: +61 3 9341 3158 Mobile: +61 422882700 Fax: +61 3 9341 3104 Facility Website Linked In Profile -----Original Message----- From: Confocal Microscopy List [mailto:[hidden email]] On Behalf Of Michelle Aloni Sent: Tuesday, 13 March 2012 11:29 AM To: [hidden email] Subject: MetaMorph versus Elements? ***** To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy ***** Dear all, If any one of you had already compared MetaMorph (we have 7.6 version) with the NIKON Elements software I would appreciate it very much if you share with me your opinion. If you used both of them, how do they compare? Which one would be your personal choice and why? Thank you ahead of time Michelle Aloni MS, HTL (ASCP) Research Specialist USC Keck School of Medicine |
*****
To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy ***** And I would second the opinions of Leoncio and Cameron. However, I think it's what software you learned first. It took awhile to get comfortable with MetaMorph, but now I know it and can use it well. Journaling is still tricky (thanks for the tutorial Cameron), but I haven't figured out NIS Elements macros at all. We have started doing image quantitation with Elements and like portions of it. Elements is WAY easier to view the multidimensional datasets, as Leoncio said. We use both MetaMorph and Elements for multi-dimensional acquisition (confocal and widefield), and my users prefer the Elements interface. Both programs really need someone who can get around inside the program for administrative purposes...our local reps are great for each. We have scheduled a training session with our Nikon rep to go over Elements in more detail, and to answer questions we've run up against. What is your local support? Best, Kathy Spencer The Scripps Research Institute -----Original Message----- From: Confocal Microscopy List [mailto:[hidden email]] On Behalf Of Vergara, Leoncio A. Sent: Monday, March 12, 2012 8:07 PM To: [hidden email] Subject: Re: MetaMorph versus Elements? ***** To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy ***** I second the opinion of Cameron, his description is almost exactly our experience. I find the automation tools in metamorph a very strong point, I almost don't do any analysis with NIS, however I find NIS better than metamorph for examining multidimensional datasets, it is very intuitive. We also use metamorph (and NIS) in combination with Image J and Imaris, I don't think there is a single package that does everything. Leoncio Vergara Assistant Professor Center for Biomedical Engineering University of Texas Medical Branch Galveston, Texas -----Original Message----- From: Confocal Microscopy List [mailto:[hidden email]] On Behalf Of Cameron Nowell Sent: Monday, March 12, 2012 8:56 PM To: [hidden email] Subject: Re: MetaMorph versus Elements? ***** To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy ***** Hi Michelle Do you want to use them for anlaysis of driving hardware? We have both but use them for different things. NIS AR is used primarily for image capture on a couple of our systems. The EDF (Extended depth of focus) is really useful for some of our imaging. We do not use NIS for image processing (other than EDF) or analysis. NIS has all the usual tools for image processing and analysis but the automation and scripting can be a bit tricky to get your head around. We use MetaMorph for capture as well (automated tile scanning and live cell imaging). For all our analysis we use a combination of MetaMorph, Fiji and Imaris. The bulk of the work is usually done in MetaMorph because its ease of scripting/automation. Part of that ease comes from what you are familiar with of course, but I find MetaMorph's journal editor to be easier to use (with better available tutorials etc) than NIS. Cost could be an issue as MetaMorph is going to be double the price of NIS (for analysis). A metamorph capture system will be more than double NIS. If you are interested in some tutorials etc for MetaMorph I am happy to send you a link to download a heap of training notes (330 page manual) and demo images I have put together over the last two years for a MetaMorph training course I co-ran here in Melbourne. The usual statements apply - no commercial interests in any of the platforms just a happy (sometimes frustrated) user. Cheers Cam Cameron J. Nowell Microscopy Manager Centre for Advanced Microscopy Ludwig Institute for Cancer Research Melbourne - Parkville Branch PO Box 2008 Royal Melbourne Hospital Victoria, 3050 AUSTRALIA Office: +61 3 9341 3158 Mobile: +61 422882700 Fax: +61 3 9341 3104 Facility Website Linked In Profile -----Original Message----- From: Confocal Microscopy List [mailto:[hidden email]] On Behalf Of Michelle Aloni Sent: Tuesday, 13 March 2012 11:29 AM To: [hidden email] Subject: MetaMorph versus Elements? ***** To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy ***** Dear all, If any one of you had already compared MetaMorph (we have 7.6 version) with the NIKON Elements software I would appreciate it very much if you share with me your opinion. If you used both of them, how do they compare? Which one would be your personal choice and why? Thank you ahead of time Michelle Aloni MS, HTL (ASCP) Research Specialist USC Keck School of Medicine |
*****
To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy ***** It's been a while since I used MetaMorph, so can't properly compare, but we're happy with NIS-Elements here. Particularly multidimensional visualisation is quick and easy to handle, and allows to produce movies easily. Image processing is pretty potent as well, particularly thresholding (adaptive etc) to quantify and make surface rendered models. I also use macros, but mostly on the hardware/acquisition side. I wish it would tie in better with ImageJ though, and/or have a light edition or something more muscular than the viewer, so people can use it at home (for weekend image analysis). No commercial interest etc. Philippe Laissue, PhD University of Essex, Colchester CO4 3SQ, UK |
Alison J. North |
In reply to this post by Michelle Aloni
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To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy ***** Hi Michelle, We use both programs here - for acquisition as well as analysis. I agree with much of what has been said so far, but I would like to add one other great thing about MetaMorph on the acquisition side - it is extremely flexible when it comes to controlling new hardware, in fact the guys at the company have written at least three new drivers for different cameras or whatever when we have asked for their help. The larger companies, such as Nikon, will probably write the necessary drivers eventually but I have been very impressed by the speed of response from the MM guys. All the best, Alison On 3/12/2012 8:28 PM, Michelle Aloni wrote: > ***** > To join, leave or search the confocal microscopy listserv, go to: > http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy > ***** > > Dear all, > > If any one of you had already compared MetaMorph (we have 7.6 version) with > the NIKON Elements software I would appreciate it very much if you share > with me your opinion. > > If you used both of them, how do they compare? Which one would be your > personal choice and why? > > Thank you ahead of time > > Michelle Aloni MS, HTL (ASCP) > Research Specialist > USC Keck School of Medicine -- Alison J. North, Ph.D., Research Associate Professor and Senior Director of the Bio-Imaging Resource Center, The Rockefeller University, 1230 York Avenue, New York, NY 10065. Tel: office ++ 212 327 7488 Tel: lab ++ 212 327 7486 Fax: ++ 212 327 7489 |
G. Esteban Fernandez |
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To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy ***** For me tech. support is a major "feature" to consider when choosing products, so I echo Alison North's point that the helpfulness and speed of the MM guys is impressive (Neil Glinksman in particular). I've sought their help with complicated journals on a number of occasions. -Esteban On Tue, Mar 13, 2012 at 11:26 AM, Alison North <[hidden email]> wrote: > ***** > To join, leave or search the confocal microscopy listserv, go to: > http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy > ***** > > Hi Michelle, > > We use both programs here - for acquisition as well as analysis. I agree > with much of what has been said so far, but I would like to add one other > great thing about MetaMorph on the acquisition side - it is extremely > flexible when it comes to controlling new hardware, in fact the guys at the > company have written at least three new drivers for different cameras or > whatever when we have asked for their help. The larger companies, such as > Nikon, will probably write the necessary drivers eventually but I have been > very impressed by the speed of response from the MM guys. > > All the best, > Alison > > > > On 3/12/2012 8:28 PM, Michelle Aloni wrote: >> >> ***** >> To join, leave or search the confocal microscopy listserv, go to: >> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy >> ***** >> >> Dear all, >> >> If any one of you had already compared MetaMorph (we have 7.6 version) >> with >> the NIKON Elements software I would appreciate it very much if you share >> with me your opinion. >> >> If you used both of them, how do they compare? Which one would be your >> personal choice and why? >> >> Thank you ahead of time >> >> Michelle Aloni MS, HTL (ASCP) >> Research Specialist >> USC Keck School of Medicine > > > -- > Alison J. North, Ph.D., > Research Associate Professor and > Senior Director of the Bio-Imaging Resource Center, > The Rockefeller University, > 1230 York Avenue, > New York, > NY 10065. > Tel: office ++ 212 327 7488 > Tel: lab ++ 212 327 7486 > Fax: ++ 212 327 7489 |
Cameron Nowell |
In reply to this post by Alison J. North
*****
To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy ***** Hi List, To follow up on Alison's point. Controlling hardware with MetaMorph can be really powerful. While it can do all the normal capture (multi-channel, time lapse etc.) you have all the power of the journal function available too. We have managed to setup journals that take overview scans of four slides, let the user draw a box around the tissue(s) of interest and it will then tile scan each of those areas without any further user intervention. You can also setup journals to change objectives etc during a time lapse as well. The possibilities are virtually endless. And as Alison says the support is great from the guys at MM. I haven't had a question they can't answer yet (must try harder). Cheers Cam -----Original Message----- From: Confocal Microscopy List [mailto:[hidden email]] On Behalf Of Alison North Sent: Wednesday, 14 March 2012 5:27 AM To: [hidden email] Subject: Re: MetaMorph versus Elements? ***** To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy ***** Hi Michelle, We use both programs here - for acquisition as well as analysis. I agree with much of what has been said so far, but I would like to add one other great thing about MetaMorph on the acquisition side - it is extremely flexible when it comes to controlling new hardware, in fact the guys at the company have written at least three new drivers for different cameras or whatever when we have asked for their help. The larger companies, such as Nikon, will probably write the necessary drivers eventually but I have been very impressed by the speed of response from the MM guys. All the best, Alison On 3/12/2012 8:28 PM, Michelle Aloni wrote: > ***** > To join, leave or search the confocal microscopy listserv, go to: > http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy > ***** > > Dear all, > > If any one of you had already compared MetaMorph (we have 7.6 version) > with the NIKON Elements software I would appreciate it very much if > you share with me your opinion. > > If you used both of them, how do they compare? Which one would be your > personal choice and why? > > Thank you ahead of time > > Michelle Aloni MS, HTL (ASCP) > Research Specialist > USC Keck School of Medicine -- Alison J. North, Ph.D., Research Associate Professor and Senior Director of the Bio-Imaging Resource Center, The Rockefeller University, 1230 York Avenue, New York, NY 10065. Tel: office ++ 212 327 7488 Tel: lab ++ 212 327 7486 Fax: ++ 212 327 7489 This communication is intended only for the named recipient and may contain information that is confidential, legally privileged or subject to copyright; the Ludwig Institute for Cancer Research Ltd does not waive any rights if you have received this communication in error. The views expressed in this communication are those of the sender and do not necessarily reflect the views of the Ludwig Institute for Cancer Research Ltd. |
leoncio vergara |
*****
To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy ***** not to high jack the thread but... realtive to controlling hardware, anybody using metamorph to run the Prairie Swept field confocal? .. ________________________________________ From: Confocal Microscopy List [[hidden email]] on behalf of Cameron Nowell [[hidden email]] Sent: Tuesday, March 13, 2012 5:58 PM To: [hidden email] Subject: Re: MetaMorph versus Elements? ***** To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy ***** Hi List, To follow up on Alison's point. Controlling hardware with MetaMorph can be really powerful. While it can do all the normal capture (multi-channel, time lapse etc.) you have all the power of the journal function available too. We have managed to setup journals that take overview scans of four slides, let the user draw a box around the tissue(s) of interest and it will then tile scan each of those areas without any further user intervention. You can also setup journals to change objectives etc during a time lapse as well. The possibilities are virtually endless. And as Alison says the support is great from the guys at MM. I haven't had a question they can't answer yet (must try harder). Cheers Cam -----Original Message----- From: Confocal Microscopy List [mailto:[hidden email]] On Behalf Of Alison North Sent: Wednesday, 14 March 2012 5:27 AM To: [hidden email] Subject: Re: MetaMorph versus Elements? ***** To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy ***** Hi Michelle, We use both programs here - for acquisition as well as analysis. I agree with much of what has been said so far, but I would like to add one other great thing about MetaMorph on the acquisition side - it is extremely flexible when it comes to controlling new hardware, in fact the guys at the company have written at least three new drivers for different cameras or whatever when we have asked for their help. The larger companies, such as Nikon, will probably write the necessary drivers eventually but I have been very impressed by the speed of response from the MM guys. All the best, Alison On 3/12/2012 8:28 PM, Michelle Aloni wrote: > ***** > To join, leave or search the confocal microscopy listserv, go to: > http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy > ***** > > Dear all, > > If any one of you had already compared MetaMorph (we have 7.6 version) > with the NIKON Elements software I would appreciate it very much if > you share with me your opinion. > > If you used both of them, how do they compare? Which one would be your > personal choice and why? > > Thank you ahead of time > > Michelle Aloni MS, HTL (ASCP) > Research Specialist > USC Keck School of Medicine -- Alison J. North, Ph.D., Research Associate Professor and Senior Director of the Bio-Imaging Resource Center, The Rockefeller University, 1230 York Avenue, New York, NY 10065. Tel: office ++ 212 327 7488 Tel: lab ++ 212 327 7486 Fax: ++ 212 327 7489 This communication is intended only for the named recipient and may contain information that is confidential, legally privileged or subject to copyright; the Ludwig Institute for Cancer Research Ltd does not waive any rights if you have received this communication in error. The views expressed in this communication are those of the sender and do not necessarily reflect the views of the Ludwig Institute for Cancer Research Ltd. |
In reply to this post by leoncio vergara
*****
To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy ***** Hi Michelle, We have a pretty similar view to Cameron, Leoncio et al. We used to use MetaMorph for capture but have since changed our licence to the Premiere off-line version as we use our single MetaMorph key more for offline image analysis on a stand-alone imaging workstation. We use Nikon's NISElements solely for image acquisition on a busy manual Nikon inverted microscope, where it's naturally well suited [rather like Zeiss Axiovision for Zeiss microscopes]. NISElements BR is easy to use [lucky as it's in-program help is pretty poor at the moment], and it offers easy background correction, fluorescence image capture, calibration and so forth [I'm talking about the expensive Basic Research BR version here, rather than the old freeware NISElements version - there's also NISElements Advanced Research AR that adds in options such as deconvolution]. We have the optional NISElements EDF tool [extended depth of focus] which is useful for compressing a 3D structure into a single 'focused' 2D picture if required. NISElements seems strongest in image acquisition rather than image analysis, compared to MetaMorph, but it might be I know MetaMorph inside out these days so it's my first port of call. Adding Nikon NISElements to a Nikon microscope for image acquisition [i.e. one supplier] might have advantages as if there's a glitch, it's all laid at one companies door - although MetaMorph will probably be more flexible with more microscope/driver options. NISElements integrates well with our Nikon Eclipse TE2000U and twin B&W/colour cameras. As we only have the single MetaMorph licence key, we also use MetaMorph to verify similar plug-ins on freeware ImageJ/Fiji are doing exactly what we expect them to do. Then users can use ImageJ instead if it's more convenient. MetaMorph generally offers better visual feedback than ImageJ on what it is doing, and I know the program so well now [we are also with v7.7 rather than new 'easier' ribbon interface MetaMorph NX] - chances are the old version 7.7 has more tools than the new NX at the moment, and rather like Photoshop CS5 you can link together actions in MetaMorph v7.7 to do more complex tasks. For 3D reconstruction, deconvolution, 3D tracking and quantitative volume measurements we have a Perkin Elmer Volocity licence key as well. We generally pay for software 'maintenance' agreements as it's only a few hundred pounds a year [this often excludes major upgrades to new versions, but includes updates/patches and support]. Support in the USA is probably even better for MetaMorph than here [UK], in that frequent free MetaMorph training courses are offered at Downington PA and Sunnyvale CA. MetaMorph v7.7 also has optional modules such 3D deconvolution - plus the 'Premiere' version has apps such as object tracking, colocalization and neurite outgrowth, which are useful to us. Relative to the cost of the microscopes and support staff, I suppose these commercial programs aren't that expensive, have very good support on call if needed, and each have their strength and weakness's. For that reason we have both NISElements and MetaMorph. We would Like to get Bitplane's Imaris to complete the set, as we have a few users here interested in neurite outgrowth, and their 3D version [Filament tracer] is more powerful than MetaMorphs and imageJ's 2D neurite apps. Other groups in our centre use ImagePro Plus for offline image analysis [to maintain consistency with collaborators elsewhere], which is similar to MetaMorph, but our Core hasn't got a licence for that. On our busy core Nikon microscope, our NISElements key hasn't much free time to do image analysis, it's mainly acquiring images with some associated processing, eg. EDF, scale bars, or background correction. Regards Keith --------------------------------------------------------------------------- Dr Keith J. Morris, Molecular Cytogenetics and Microscopy Core, Laboratory 00/069 and 00/070, The Wellcome Trust Centre for Human Genetics, Roosevelt Drive, Oxford OX3 7BN, United Kingdom. Telephone: +44 (0)1865 287568 Email: [hidden email] Web-pages: http://www.well.ox.ac.uk/microscopy-core http://www.well.ox.ac.uk/nikon-microscope and http://www.well.ox.ac.uk/metamorph -----Original Message----- From: Confocal Microscopy List [mailto:[hidden email]] On Behalf Of Vergara, Leoncio A. Sent: 13 March 2012 03:07 To: [hidden email] Subject: Re: MetaMorph versus Elements? ***** To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy ***** I second the opinion of Cameron, his description is almost exactly our experience. I find the automation tools in metamorph a very strong point, I almost don't do any analysis with NIS, however I find NIS better than metamorph for examining multidimensional datasets, it is very intuitive. We also use metamorph (and NIS) in combination with Image J and Imaris, I don't think there is a single package that does everything. Leoncio Vergara Assistant Professor Center for Biomedical Engineering University of Texas Medical Branch Galveston, Texas -----Original Message----- From: Confocal Microscopy List [mailto:[hidden email]] On Behalf Of Cameron Nowell Sent: Monday, March 12, 2012 8:56 PM To: [hidden email] Subject: Re: MetaMorph versus Elements? ***** To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy ***** Hi Michelle Do you want to use them for anlaysis of driving hardware? We have both but use them for different things. NIS AR is used primarily for image capture on a couple of our systems. The EDF (Extended depth of focus) is really useful for some of our imaging. We do not use NIS for image processing (other than EDF) or analysis. NIS has all the usual tools for image processing and analysis but the automation and scripting can be a bit tricky to get your head around. We use MetaMorph for capture as well (automated tile scanning and live cell imaging). For all our analysis we use a combination of MetaMorph, Fiji and Imaris. The bulk of the work is usually done in MetaMorph because its ease of scripting/automation. Part of that ease comes from what you are familiar with of course, but I find MetaMorph's journal editor to be easier to use (with better available tutorials etc) than NIS. Cost could be an issue as MetaMorph is going to be double the price of NIS (for analysis). A metamorph capture system will be more than double NIS. If you are interested in some tutorials etc for MetaMorph I am happy to send you a link to download a heap of training notes (330 page manual) and demo images I have put together over the last two years for a MetaMorph training course I co-ran here in Melbourne. The usual statements apply - no commercial interests in any of the platforms just a happy (sometimes frustrated) user. Cheers Cam Cameron J. Nowell Microscopy Manager Centre for Advanced Microscopy Ludwig Institute for Cancer Research Melbourne - Parkville Branch PO Box 2008 Royal Melbourne Hospital Victoria, 3050 AUSTRALIA Office: +61 3 9341 3158 Mobile: +61 422882700 Fax: +61 3 9341 3104 Facility Website Linked In Profile -----Original Message----- From: Confocal Microscopy List [mailto:[hidden email]] On Behalf Of Michelle Aloni Sent: Tuesday, 13 March 2012 11:29 AM To: [hidden email] Subject: MetaMorph versus Elements? ***** To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy ***** Dear all, If any one of you had already compared MetaMorph (we have 7.6 version) with the NIKON Elements software I would appreciate it very much if you share with me your opinion. If you used both of them, how do they compare? Which one would be your personal choice and why? Thank you ahead of time Michelle Aloni MS, HTL (ASCP) Research Specialist USC Keck School of Medicine |
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