Micro-Dissection with Plant Tissue- Any thoughts?

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Kimberly Young Kimberly Young
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Micro-Dissection with Plant Tissue- Any thoughts?

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Hello Microscopists,
We have a group interested in doing micro-dissection on a PALM system of
ours. Does anyone have any experience or tips for microdissection in plant
tissue? The samples are about 5 cells thick, and the group was hoping to do
the dissection of live cells, but is this possible? Is fixing necessary? If
so, what method is the best and how does this affect the dissection?
Thanks for your input,
Kimberly Young

McGill Imaging Facility
Montreal, Canada
Rosemary.White Rosemary.White
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Re: Micro-Dissection with Plant Tissue- Any thoughts?

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Hi Kimberly,

What is the aim of the microdissection? RNA extraction, DNA? or??
What is the tissue - tiny cells, large cells, meristematic or highly
vacuolate, etc.?

This and other info will influence your approach.

cheers,
Rosemary

Dr Rosemary White
CSIRO Plant Industry
GPO Box 1600
Canberra, ACT 2601
Australia

T 61 2 6246 5475
F 61 2 6246 5334
E [hidden email]


On 12/06/12 8:01 AM, "Kimberly Young" <[hidden email]> wrote:

>*****
>To join, leave or search the confocal microscopy listserv, go to:
>http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
>*****
>
>Hello Microscopists,
>We have a group interested in doing micro-dissection on a PALM system of
>ours. Does anyone have any experience or tips for microdissection in plant
>tissue? The samples are about 5 cells thick, and the group was hoping to
>do
>the dissection of live cells, but is this possible? Is fixing necessary?
>If
>so, what method is the best and how does this affect the dissection?
>Thanks for your input,
>Kimberly Young
>
>McGill Imaging Facility
>Montreal, Canada
Graham Wright Graham Wright
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Re: Micro-Dissection with Plant Tissue- Any thoughts?

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Hi Kimberly,

I was once, very helpfully, given a collection of papers and articles by my
local Zeiss applications specialist on the subject of plant laser capture
microdissection (thanks to Samuel Ko for this). The list is below,
hopefully this helps. Let me know if you're unable to access any.

Angeles et al 2006 Planta 224_228
Connolly 2001 Biophotonics International 61-63
Fominaya et al 2005 Cytogenet Genome Res 109_8
Hobza et al 2004 Chrom Res 12_245
Ivashikina et al 2003 Plant J 36_931
Kehr 2003 Curr Op in Plant Biol 6_617
Levy 2003 Appl Env Microbiol 69_6250
Matsunaga et al 1999 Plant J 20_371
Meimberg et al 2003 Biotechniques 34
Polster et al 2006 Physiologia Platarum 128_163
Ramsay et al 2004 Mol Plant Path 5_587
Schad et al 2005 Electrophoresis 26
Schad et al 2005 Plant Meth 1_2
Zhang et al 2007 PLoS Genetics 3_e101

Regards,
Graham

On 12 June 2012 06:01, Kimberly Young <[hidden email]> wrote:

> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> *****
>
> Hello Microscopists,
> We have a group interested in doing micro-dissection on a PALM system of
> ours. Does anyone have any experience or tips for microdissection in plant
> tissue? The samples are about 5 cells thick, and the group was hoping to do
> the dissection of live cells, but is this possible? Is fixing necessary? If
> so, what method is the best and how does this affect the dissection?
> Thanks for your input,
> Kimberly Young
>
> McGill Imaging Facility
> Montreal, Canada
>