Kimberly Young |
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To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy ***** Hello Microscopists, We have a group interested in doing micro-dissection on a PALM system of ours. Does anyone have any experience or tips for microdissection in plant tissue? The samples are about 5 cells thick, and the group was hoping to do the dissection of live cells, but is this possible? Is fixing necessary? If so, what method is the best and how does this affect the dissection? Thanks for your input, Kimberly Young McGill Imaging Facility Montreal, Canada |
Rosemary.White |
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To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy ***** Hi Kimberly, What is the aim of the microdissection? RNA extraction, DNA? or?? What is the tissue - tiny cells, large cells, meristematic or highly vacuolate, etc.? This and other info will influence your approach. cheers, Rosemary Dr Rosemary White CSIRO Plant Industry GPO Box 1600 Canberra, ACT 2601 Australia T 61 2 6246 5475 F 61 2 6246 5334 E [hidden email] On 12/06/12 8:01 AM, "Kimberly Young" <[hidden email]> wrote: >***** >To join, leave or search the confocal microscopy listserv, go to: >http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy >***** > >Hello Microscopists, >We have a group interested in doing micro-dissection on a PALM system of >ours. Does anyone have any experience or tips for microdissection in plant >tissue? The samples are about 5 cells thick, and the group was hoping to >do >the dissection of live cells, but is this possible? Is fixing necessary? >If >so, what method is the best and how does this affect the dissection? >Thanks for your input, >Kimberly Young > >McGill Imaging Facility >Montreal, Canada |
Graham Wright |
In reply to this post by Kimberly Young
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To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy ***** Hi Kimberly, I was once, very helpfully, given a collection of papers and articles by my local Zeiss applications specialist on the subject of plant laser capture microdissection (thanks to Samuel Ko for this). The list is below, hopefully this helps. Let me know if you're unable to access any. Angeles et al 2006 Planta 224_228 Connolly 2001 Biophotonics International 61-63 Fominaya et al 2005 Cytogenet Genome Res 109_8 Hobza et al 2004 Chrom Res 12_245 Ivashikina et al 2003 Plant J 36_931 Kehr 2003 Curr Op in Plant Biol 6_617 Levy 2003 Appl Env Microbiol 69_6250 Matsunaga et al 1999 Plant J 20_371 Meimberg et al 2003 Biotechniques 34 Polster et al 2006 Physiologia Platarum 128_163 Ramsay et al 2004 Mol Plant Path 5_587 Schad et al 2005 Electrophoresis 26 Schad et al 2005 Plant Meth 1_2 Zhang et al 2007 PLoS Genetics 3_e101 Regards, Graham On 12 June 2012 06:01, Kimberly Young <[hidden email]> wrote: > ***** > To join, leave or search the confocal microscopy listserv, go to: > http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy > ***** > > Hello Microscopists, > We have a group interested in doing micro-dissection on a PALM system of > ours. Does anyone have any experience or tips for microdissection in plant > tissue? The samples are about 5 cells thick, and the group was hoping to do > the dissection of live cells, but is this possible? Is fixing necessary? If > so, what method is the best and how does this affect the dissection? > Thanks for your input, > Kimberly Young > > McGill Imaging Facility > Montreal, Canada > |
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