Microscope Stage Incubators

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Charles Matthew Blaha Charles Matthew Blaha
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Microscope Stage Incubators

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Hey everyone..this is my first time using this server.  Sorry if this  
message was sent twice..i am still unsure how this posting thing  
works.  I am a new research assistant working in the department of  
bioengineering at Stanford University and I have a few questions  
regarding Microscope Stage Incubators.

My group plans to study live corneal epithelial cell migration and  
proliferation (and possibly other cell characteristics) on transparent  
hydrogels over a period of 1 to 4 days.  We plan to use 10 ro 20x  
objectives so it will be a long working distance.  As an undergrad, I  
performed live cell imaging with a microscope stage incubator on a  
leica microscope, so I have some experiene in the area. I am in charge  
of determining which microscope stage incubator is best for our  
application.  We have a Nikon TE300 with an Applied Scientific  
Instruments MS-2000 stage.

There appears to be two different microscope stage incubation systems:  
hood incubators and stage incubators.  I have spoken to a variety of  
companies with conflicting recommendations, although I am leaning  
towards a Stage incubator from OKO-labs.  Any suggestions if a hood or  
a stage incubator is better?  Has anyone had success or been  
disapointed with certain companies hoods or stage incubators?

I would greatly appreciate any help.

Sincerely,

Charles Blaha M.S.
Department of Bioengineering
Stanford University