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http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocalHey everyone..this is my first time using this server. Sorry if this
message was sent twice..i am still unsure how this posting thing
works. I am a new research assistant working in the department of
bioengineering at Stanford University and I have a few questions
regarding Microscope Stage Incubators.
My group plans to study live corneal epithelial cell migration and
proliferation (and possibly other cell characteristics) on transparent
hydrogels over a period of 1 to 4 days. We plan to use 10 ro 20x
objectives so it will be a long working distance. As an undergrad, I
performed live cell imaging with a microscope stage incubator on a
leica microscope, so I have some experiene in the area. I am in charge
of determining which microscope stage incubator is best for our
application. We have a Nikon TE300 with an Applied Scientific
Instruments MS-2000 stage.
There appears to be two different microscope stage incubation systems:
hood incubators and stage incubators. I have spoken to a variety of
companies with conflicting recommendations, although I am leaning
towards a Stage incubator from OKO-labs. Any suggestions if a hood or
a stage incubator is better? Has anyone had success or been
disapointed with certain companies hoods or stage incubators?
I would greatly appreciate any help.
Sincerely,
Charles Blaha M.S.
Department of Bioengineering
Stanford University