Microscope darkening for 2P NDD imaging

[Wendy Salmon]

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Hi All,
I have a 2P set up on an inverted microscope and need to add some additional light blocking for using the NDDs. I know people use clothes draped over the scope but my search of the archives didn't turn up any specifics on what type of cloth or where it can be purchased. Does anyone here have suggestions?

Thanks!
Wendy

~~~~~~~~~~~~~~~~~~~~~~~
Wendy Salmon
Light Microscopy Specialist
Whitehead Institute for Biomedical Research
W.M. Keck Imaging Facility
9 Cambridge Center, Rm 447
Cambridge, MA 02142
c: 617-429-0158
e: [hidden email]
w: http://staffa.wi.mit.edu/microscopy/ 

[mcammer]

Some people use heavy duty ("contractor") garbage bags cut into sheets but I prefer the more flexible and less staticky black plastic cloth sold by ThorLabs.  http://thorlabs.com/NewGroupPage9.cfm?ObjectGroup_ID=190 
________________________________________________________
Michael Cammer, Assistant Research Scientist
Skirball Institute of Biomolecular Medicine
Lab: [phone]  Cell: [phone]

-----Original Message-----
From: Confocal Microscopy List [mailto:[hidden email]] On Behalf Of Wendy Salmon
Sent: Tuesday, July 17, 2012 3:41 PM
To: [hidden email]
Subject: Microscope darkening for 2P NDD imaging

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Hi All,
I have a 2P set up on an inverted microscope and need to add some additional light blocking for using the NDDs. I know people use clothes draped over the scope but my search of the archives didn't turn up any specifics on what type of cloth or where it can be purchased. Does anyone here have suggestions?

Thanks!
Wendy

~~~~~~~~~~~~~~~~~~~~~~~
Wendy Salmon
Light Microscopy Specialist
Whitehead Institute for Biomedical Research W.M. Keck Imaging Facility
9 Cambridge Center, Rm 447
Cambridge, MA 02142
c: 617-429-0158
e: [hidden email]
w: http://staffa.wi.mit.edu/microscopy/ 

[Watkins, Simon C]

The absolute best trick we have for managing almost all the spurious room light when doing 2p is to block the light coming from the monitor using 3M security screens that you see folks using in airports etc.  You can get these for a 30 inch monitor for a few hundred dollars.  This removes the need for all those silly tinfoil/curtain drapes that we all use(d) to stop the light getting to the NDDs...You do have to be front on to the monitor to get a good look at whats going on, and it is a bit dimmer but it works really well.  Beyond that its black electrical tape over all the idiot lights that apparently the vendors feel we need :-)
S

Simon C. Watkins Ph.D, FRC Path
Professor and Vice Chair Cell Biology
Professor Immunology
Director Center for Biologic Imaging
BSTS 225
University of Pittsburgh
3500 Terrace St
Pittsburgh PA 15261
412-352-2277
www.cbi.pitt.edu


-----Original Message-----
From: Confocal Microscopy List [mailto:[hidden email]] On Behalf Of Wendy Salmon
Sent: Tuesday, July 17, 2012 3:41 PM
To: [hidden email]
Subject: Microscope darkening for 2P NDD imaging

*****
To join, leave or search the confocal microscopy listserv, go to:
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
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Hi All,
I have a 2P set up on an inverted microscope and need to add some additional light blocking for using the NDDs. I know people use clothes draped over the scope but my search of the archives didn't turn up any specifics on what type of cloth or where it can be purchased. Does anyone here have suggestions?

Thanks!
Wendy

~~~~~~~~~~~~~~~~~~~~~~~
Wendy Salmon
Light Microscopy Specialist
Whitehead Institute for Biomedical Research W.M. Keck Imaging Facility
9 Cambridge Center, Rm 447
Cambridge, MA 02142
c: 617-429-0158
e: [hidden email]
w: http://staffa.wi.mit.edu/microscopy/ 

[Craig Brideau]

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Oh yeah, we have little black patches of tape over so many little blinking
lights in our lab.  It is actually quite absurd.

Craig


On Tue, Jul 17, 2012 at 2:52 PM, Watkins, Simon C <[hidden email]> wrote:

>   Beyond that its black electrical tape over all the idiot lights that
> apparently the vendors feel we need :-)
> S
>
> Simon C. Watkins Ph.D, FRC Path
> Professor and Vice Chair Cell Biology
> Professor Immunology
> Director Center for Biologic Imaging
> BSTS 225
> University of Pittsburgh
> 3500 Terrace St
> Pittsburgh PA 15261
> 412-352-2277
> www.cbi.pitt.edu
>
>
> -----Original Message-----
> From: Confocal Microscopy List [mailto:[hidden email]]
> On Behalf Of Wendy Salmon
> Sent: Tuesday, July 17, 2012 3:41 PM
> To: [hidden email]
> Subject: Microscope darkening for 2P NDD imaging
>
> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> *****
>
> Hi All,
> I have a 2P set up on an inverted microscope and need to add some
> additional light blocking for using the NDDs. I know people use clothes
> draped over the scope but my search of the archives didn't turn up any
> specifics on what type of cloth or where it can be purchased. Does anyone
> here have suggestions?
>
> Thanks!
> Wendy
>
> ~~~~~~~~~~~~~~~~~~~~~~~
> Wendy Salmon
> Light Microscopy Specialist
> Whitehead Institute for Biomedical Research W.M. Keck Imaging Facility
> 9 Cambridge Center, Rm 447
> Cambridge, MA 02142
> c: 617-429-0158
> e: [hidden email]
> w: http://staffa.wi.mit.edu/microscopy/
>

[Gary G. Li-2]

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Hi Wendy Salmon,
I suggested a facility user to cut and tape a new black garbage bag on
the upright microscope when NDDs were used for a multiphoton
experiment.
The feedback is:  It works amazingly well.
Regards
Gary G Li, PhD
Baltimore, MD 21224


On Tue, Jul 17, 2012 at 3:41 PM, Wendy Salmon <[hidden email]> wrote:

> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> *****
>
> Hi All,
> I have a 2P set up on an inverted microscope and need to add some additional light blocking for using the NDDs. I know people use clothes draped over the scope but my search of the archives didn't turn up any specifics on what type of cloth or where it can be purchased. Does anyone here have suggestions?
>
> Thanks!
> Wendy
>
> ~~~~~~~~~~~~~~~~~~~~~~~
> Wendy Salmon
> Light Microscopy Specialist
> Whitehead Institute for Biomedical Research
> W.M. Keck Imaging Facility
> 9 Cambridge Center, Rm 447
> Cambridge, MA 02142
> c: 617-429-0158
> e: [hidden email]
> w: http://staffa.wi.mit.edu/microscopy/