Microscopy Core Facilities: Results of an International Survey

[Periasamy, Ammasi (ap3t)]

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Dear All
If you are interested in the core facility operation, please look at the recent international core facility survey, published in Microscopy today.
http://www.microscopy-today.com/index.jsf;jsessionid=B775FCB1AC6B0F4E6053446D42840FC3
Microscopy Core Facilities: Results of an International Survey
Horst Wallrabe, Ammasi Periasamy, Masilamani Elangovan,
Microscopy Today,  Volume22,   Issue 02  Mar 2014,  pp 36 - 45
Published online by Cambridge University Press  Mar 11 2014
Best wishes,
Ammasi
Dr. Ammasi Periasamy
Professor & Center Director
http://www.kcci.virginia.edu/contact/peri.php
FedEx or UPS Shipping Address:
W.M. Keck Center for Cellular Imaging (PLSB 005)
University of Virginia
Biology, Gilmer Hall, 485 McCormick Rd.
Charlottesville, VA 22904, USA
Phone: [phone] or 982-4869
Fax: [phone]
Messenger Mail: P.O. Box 400328
E-mail: [hidden email]<mailto:[hidden email]>
FRET Workshop: http://www.kcci.virginia.edu/workshop/index.php

[Craig Brideau]

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Thanks for the link! I got the paper copy, but was lamenting not having a
digital version.  I was very interested in the results in terms of
distributions of systems. FYI, to download the article click on the
magazine cover on the page Ammasi's link sends you to. The Core article is
about halfway down the next page (search for 'Core' on the page).

Craig Brideau


On Tue, Apr 8, 2014 at 8:50 AM, Periasamy, Ammasi (ap3t) <
[hidden email]> wrote:

> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> Post images on http://www.imgur.com and include the link in your posting.
> *****
>
> Dear All
> If you are interested in the core facility operation, please look at the
> recent international core facility survey, published in Microscopy today.
>
> http://www.microscopy-today.com/index.jsf;jsessionid=B775FCB1AC6B0F4E6053446D42840FC3
> Microscopy Core Facilities: Results of an International Survey
> Horst Wallrabe, Ammasi Periasamy, Masilamani Elangovan,
> Microscopy Today,  Volume22,   Issue 02  Mar 2014,  pp 36 - 45
> Published online by Cambridge University Press  Mar 11 2014
> Best wishes,
> Ammasi
> Dr. Ammasi Periasamy
> Professor & Center Director
> http://www.kcci.virginia.edu/contact/peri.php
> FedEx or UPS Shipping Address:
> W.M. Keck Center for Cellular Imaging (PLSB 005)
> University of Virginia
> Biology, Gilmer Hall, 485 McCormick Rd.
> Charlottesville, VA 22904, USA
> Phone: [phone] or 982-4869
> Fax: [phone]
> Messenger Mail: P.O. Box 400328
> E-mail: [hidden email]<mailto:[hidden email]>
> FRET Workshop: http://www.kcci.virginia.edu/workshop/index.php
>

[Ke Peng]

Hello Everybody:
Can somebody recommend a specific antibody of Dynein heavy chain (or other subunits but preferentially the heavy chain) for Immunofluorescence analysis?
I have been looking at the available options from several (prestigious) companies but feel that with this large number of 'promising' options it is not very easy to choose.
I will be very grateful if someone can share some experience on this.
Many thanks in advance.
Regards,
Aro    

[Marin Barisic]

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Aro,
this one was quite good for me:
Dynein Heavy Chain 1 rabbit polyclonal antibody: HPA003742, Sigma.
I was using methanol fixation and diluting it 1:50 - 1:100.
You can take a look at the images in this paper:
http://www.ncbi.nlm.nih.gov/pubmed/20427577
Regards,
Marin

On Apr 9, 2014, at 5:02 PM, PengKe wrote:

> Hello Everybody:
> Can somebody recommend a specific antibody of Dynein heavy chain (or other subunits but preferentially the heavy chain) for Immunofluorescence analysis?
> I have been looking at the available options from several (prestigious) companies but feel that with this large number of 'promising' options it is not very easy to choose.
> I will be very grateful if someone can share some experience on this.
> Many thanks in advance.
> Regards,
> Aro    

[Ke Peng]

Thanks a lot Martin. I will order this one and give it a try.
I had a look at your figures. The punctate signals in the cytoplasm seems to be a little bit less than I expected since Dynein is the major retro-grade motor protein. But I had no experience with Dynein and cytoskeleton was not really relevant for me in the past so my expectation has no solid ground. I think I will try and see.
Thanks again.
Aro

> Date: Wed, 9 Apr 2014 18:16:18 +0100
> From: [hidden email]
> Subject: Re: Specific antibody of Dynein heavy chain in Immunofluorescence analysis
> To: [hidden email]
>
> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> Post images on http://www.imgur.com and include the link in your posting.
> *****
>
> Aro,
> this one was quite good for me:
> Dynein Heavy Chain 1 rabbit polyclonal antibody: HPA003742, Sigma.
> I was using methanol fixation and diluting it 1:50 - 1:100.
> You can take a look at the images in this paper:
> http://www.ncbi.nlm.nih.gov/pubmed/20427577
> Regards,
> Marin
>
> On Apr 9, 2014, at 5:02 PM, PengKe wrote:
>
> > Hello Everybody:
> > Can somebody recommend a specific antibody of Dynein heavy chain (or other subunits but preferentially the heavy chain) for Immunofluorescence analysis?
> > I have been looking at the available options from several (prestigious) companies but feel that with this large number of 'promising' options it is not very easy to choose.
> > I will be very grateful if someone can share some experience on this.
> > Many thanks in advance.
> > Regards,
> > Aro    

     

[Marin Barisic]

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I was looking at mitotic cells, that's why you see the punctate signal, since it localizes at kinetochores and mitotic spindle at this stage.
Good luck with your stainings!

On Apr 9, 2014, at 10:06 PM, PengKe wrote:

> Thanks a lot Martin. I will order this one and give it a try.
> I had a look at your figures. The punctate signals in the cytoplasm seems to be a little bit less than I expected since Dynein is the major retro-grade motor protein. But I had no experience with Dynein and cytoskeleton was not really relevant for me in the past so my expectation has no solid ground. I think I will try and see.
> Thanks again.
> Aro
>
>> Date: Wed, 9 Apr 2014 18:16:18 +0100
>> From: [hidden email]
>> Subject: Re: Specific antibody of Dynein heavy chain in Immunofluorescence analysis
>> To: [hidden email]
>>
>> *****
>> To join, leave or search the confocal microscopy listserv, go to:
>> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
>> Post images on http://www.imgur.com and include the link in your posting.
>> *****
>>
>> Aro,
>> this one was quite good for me:
>> Dynein Heavy Chain 1 rabbit polyclonal antibody: HPA003742, Sigma.
>> I was using methanol fixation and diluting it 1:50 - 1:100.
>> You can take a look at the images in this paper:
>> http://www.ncbi.nlm.nih.gov/pubmed/20427577
>> Regards,
>> Marin
>>
>> On Apr 9, 2014, at 5:02 PM, PengKe wrote:
>>
>>> Hello Everybody:
>>> Can somebody recommend a specific antibody of Dynein heavy chain (or other subunits but preferentially the heavy chain) for Immunofluorescence analysis?
>>> I have been looking at the available options from several (prestigious) companies but feel that with this large number of 'promising' options it is not very easy to choose.
>>> I will be very grateful if someone can share some experience on this.
>>> Many thanks in advance.
>>> Regards,
>>> Aro    
>