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Quantitative Imaging: From Cells to Molecules
Cold Spring Harbor Laboratory
April 5-18, 2016
Applications due January 15, 2016
Online application:
https://meetings.cshl.edu/courseshome.aspxOrganized by:
Jennifer Waters, Harvard Medical School
Hunter Elliott, Harvard Medical School
Talley Lambert, Harvard Medical School
Torsten Wittmann, UCSF
Combining careful image acquisition with computational analysis allows us to
extract quantitative data from light microscopy images that is far more informative
and reliable than what can be seen by eye. This course will focus on advanced
quantitative fluorescence microscopy techniques used for imaging a range of
biological specimens, from cells to single molecules. The course is designed for cell
and molecular biologists with little or no microscopy experience who wish to begin
utilizing microscopy in their own research. Students will gain a theoretical
understanding of, and hands-on experience with, state-of-the-art equipment used
in quantitative fluorescence microscopy, including: wide-field fluorescence
microscopy, laser scanning and spinning disk confocal microscopy, total internal
fluorescence microscopy (TIRF), super-resolution methods (structured illumination,
STED, STORM and PALM) and digital image processing and analysis. Students will
learn how to design and implement a wide range of imaging experiments using
these techniques. Students will also learn fundamental image processing,
segmentation and analysis techniques using a variety of commercial and open
source software packages. Students will use these image acquisition and analysis
techniques to address specific quantitative questions and then discuss the results
as a group, learning to troubleshoot the common problems that occur in the course
of a quantitative imaging experiment. Among the lectures presented are:
quantitative microscopy basics, transmitted light microscopy, image segmentation,
image analysis, CCD & sCMOS cameras, confocal microscopy, multi-photon
microscopy, deconvolution, TIRF, imaging ratio-metric “biosensors” (including
FRET), light sheet microscopy and super-resolution techniques. Students will also
learn guidelines for choosing fluorescent proteins, and work with live samples
requiring environmental control.
Additional Lecturers for 2016:
Julie Canman, Columbia University
Elizabeth M.C. Hillman, Columbia University
Bo Huang, University of California, San Francisco
Nathan Shaner, The Scintillon Institute
Clare Waterman, National Institutes of Health
More info and online application:
https://meetings.cshl.edu/courseshome.aspx
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