Dear confocal list,
About a month ago I po
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Dear confocal list,
About a month ago I posted a grammatical question on using "microscopy" or "microscopies" ("We’ve written a paper whose title contains the phrase ‘[...] using confocal and light sheet fluorescence microscopies’ ... A reviewer suggests replacing 'microscopies' with “microscopy.'" ) It was great to see many interesting responses -- thanks! -- including even an on-line poll (Lars Engstrom). Most people preferred "Microscopy;" some thought "Microscopies" may be accurate but sounds odd; and some, Solomon-like, suggested avoiding the pitfalls of either and using instead something like "Microscopy techniques." I decided on this third path: "... confocal and light sheet fluorescence microscopy techniques."
Not that it's relevant to the grammar, but if anyone is curious, the full title / abstract of the paper are:
Comparing phototoxicity during the development of a zebrafish craniofacial bone using confocal and light sheet fluorescence microscopy techniques.
The combination of genetically encoded fluorescent proteins and three-dimensional imaging enables cell-type-specific studies of embryogenesis. Light sheet microscopy, in which fluorescence excitation is provided by a plane of laser light, is an appealing approach to live imaging due to its high speed and efficient use of photons. While the advantages of rapid imaging are apparent from recent work, the importance of low light levels to studies of development is not well established. We examine the zebrafish opercle, a craniofacial bone that exhibits pronounced shape changes at early developmental stages, using both spinning disk confocal and light sheet microscopies of fluorescent osteoblast cells. We find normal and aberrant opercle morphologies for specimens imaged with short time intervals using light sheet and spinning disk confocal microscopies, respectively, under equivalent exposure conditions over developmentally-relevant time
scales. Quantification of shapes reveals that the differently imaged specimens travel along distinct trajectories in morphological space. [In press, Journal of Biophotonics. I'm happy to send preprints. Email:
[hidden email]].
Thanks!
Raghu
--
Raghuveer Parthasarathy
[hidden email]
Associate Professor
Department of Physics
1274 University of Oregon
Eugene, OR 97403-1274
http://physics.uoregon.edu/~raghu/
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