Microscopy techniques and tools for 8th and 9th graders
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Mayandi Sivaguru |
Microscopy techniques and tools for 8th and 9th graders
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Search the CONFOCAL archive at
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Dear members of the list, We are planning to offer a one week summer
program for 8th and 9th graders on Optical microscopy and imaging
techniques. I kindly request your input on any available book or
literature at this level with basic geometrical optics, optical path,
image formation, contrasting techniques including polarization
information and any available easy to understand tools and kits
(especially for diffraction patterns) that could be used for the students
to make mini projects.
Thanking you in advance and I greatly appreciate your suggestions and recommendations. Shiv Microscopy Facility Manager 8, Institute for Genomic Biology University of Illinois at Urbana-Champaign 1206 West Gregory Dr. Urbana, IL 61801 USA Office: 217.333.1214 Fax: 217.244.2496 [hidden email] http://core.igb.uiuc.edu |
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Julio Vazquez |
Re: Microscopy techniques and tools for 8th and 9th graders
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Search the CONFOCAL archive at
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Hello Shiv, One book that might fit nicely between the ones I already mentioned is "Introduction to Light Microscopy" by Bradbury and Bracegirdle. Microscopy Handbook Series Vol 42 (Springer). It's 100 pages, and might be just the level you need for your students. Julio. = On Feb 14, 2008, at 7:21 AM, Mayandi Sivaguru wrote: Search the CONFOCAL archive at http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal Dear members of the list, We are planning to offer a one week summer program for 8th and 9th graders on “Optical microscopy and imaging techniques”. I kindly request your input on any available book or literature at this level with basic geometrical optics, optical path, image formation, contrasting techniques including polarization information and any available easy to understand tools and kits (especially for diffraction patterns) that could be used for the students to make mini projects. |
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Julio Vazquez |
Re: Microscopy techniques and tools for 8th and 9th graders
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In reply to this post by Mayandi Sivaguru
Search the CONFOCAL archive at
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Hello Shiv, I am sending my first post again... it had been rejected because of a derelict attachment... Julio = Dear Shiv, I really like the introductory book by Doug Murphy: Fundamentals of Light Microscopy and Electronic Imaging.
-- Julio Vazquez, Fred Hutchinson Cancer Research Center Seattle, WA 98109-1024 = On Feb 14, 2008, at 7:21 AM, Mayandi Sivaguru wrote: Search the CONFOCAL archive at http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal Dear members of the list, We are planning to offer a one week summer program for 8th and 9th graders on “Optical microscopy and imaging techniques”. I kindly request your input on any available book or literature at this level with basic geometrical optics, optical path, image formation, contrasting techniques including polarization information and any available easy to understand tools and kits (especially for diffraction patterns) that could be used for the students to make mini projects. Thanking you in advance and I greatly appreciate your suggestions and recommendations. Shiv On Feb 14, 2008, at 7:21 AM, Mayandi Sivaguru wrote: Search the CONFOCAL archive at http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal Dear members of the list, We are planning to offer a one week summer program for 8th and 9th graders on “Optical microscopy and imaging techniques”. I kindly request your input on any available book or literature at this level with basic geometrical optics, optical path, image formation, contrasting techniques including polarization information and any available easy to understand tools and kits (especially for diffraction patterns) that could be used for the students to make mini projects. |
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In reply to this post by Mayandi Sivaguru
Search the CONFOCAL archive at
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Please take a look at Caroline Schooley’s work at Project
Micro, an outreach of the Microscopy Society of America. There is an
extensive bibliography there, reviewing books, videos and other materials aimed
at school-aged children. See: http://www.microscopy.org/ProjectMicro/PMHomePage.html You also might want to look at: http://www.lawrencehallofscience.org/gems/GEMmicro.html
Doug ^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^ Douglas W. Cromey, M.S. - Assistant Scientific Investigator Dept. of Cell Biology & Anatomy, University of Arizona 1501 N. Campbell Ave, Tucson, AZ 85724-5044 USA office: AHSC
4212 email: [hidden email] voice: 520-626-2824
fax: 520-626-2097 http://swehsc.pharmacy.arizona.edu/exppath/ Home of: "Microscopy and Imaging Resources on the WWW" From: Confocal Microscopy
List [mailto:[hidden email]] On Behalf Of Mayandi
Sivaguru Search the CONFOCAL archive at
http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal Dear members of the list, We are planning to offer a one
week summer program for 8th and 9th graders on “Optical microscopy and
imaging techniques”. I kindly request your input on any available book or
literature at this level with basic geometrical optics, optical path, image
formation, contrasting techniques including polarization information and any
available easy to understand tools and kits (especially for diffraction
patterns) that could be used for the students to make mini projects. Mayandi Sivaguru, PhD, PhD |
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PattyJansma |
Re: Microscopy techniques and tools for 8th and 9th graders
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In reply to this post by Mayandi Sivaguru
Search the CONFOCAL archive at
http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
I would also recommend Caroline Schooley's work on Project Micro. http://www.microscopy.org/ProjectMicro/PMHomePage.html The Private Eye by Kerry Ruef is also very useful. It is more on critical thinking using a 5x loupe as a tool. Patty Patty Jansma Imaging Facility Manager ARLDN University of AZ Tucson, AZ 85721 At 08:21 AM 2/14/2008, you wrote: Search the CONFOCAL archive at http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal Dear members of the list, We are planning to offer a one week summer program for 8th and 9th graders on Optical microscopy and imaging techniques. I kindly request your input on any available book or literature at this level with basic geometrical optics, optical path, image formation, contrasting techniques including polarization information and any available easy to understand tools and kits (especially for diffraction patterns) that could be used for the students to make mini projects. |
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Barbara Foster |
Re: Microscopy techniques and tools for 8th and 9th graders
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In reply to this post by Mayandi Sivaguru
Search the CONFOCAL archive at
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Hi, Mayandi
Optimizing Light Microscopy has tons of little experiments scattered through it. We have a few copies (less than 10) left. Also, don't forget Caroline Schooley's wonderful GEM progam. I'm sure that there are a lot of things there. (Caroline Schooley<[hidden email]>) Very there are three experiments which I strongly support: a. Use of hand lens to differentiate between object and the image formed by a lens. I have my students find the focal length by capturing the image of the over head lights on the desk top. Once they know the focal length, have them look through the lens at their fingernail at the following distances: inside the focal length; then, holding lens steady, move finger further out. At focal length finger will disappear; beyond focal length it will reappear, but upside down. You can tease them about "At no time did your finger leave your hand!" This expt is great for talking about the 4 General Cases of Lenses, finding focal point, measuring focal length, On-axis versus off-axis imaging, and focal plane) b. For diffraction, you can actually just shine light past the edge of a single-edge razor blade and image on the wall. Also, if you have the kids VERY gently separate their fingers, hold their hands about 10" from their faces, and view a distant light source, they will see Fraunhofer fringes between their fingers. c. Then there is Pol... this is a real treat. I have a big set of polars (I think you can get them from Edmund Scientific) that are about 10" x 10". I put one down on an overhead projector (you need to use the old-fashioned ones with the big boxy base) then use something like a shoe box to create a spacer, then put the other one on top, with a weight. That gives me a little "Pol Theater" in which I can do all sorts of experiments. The result is projected on the wall or a screen I usually start with something like a clear plastic party plate that has a pattern embossed. Show them the plate in regular light (clear, no color) then between crossed polars, projected onto the screen. The results are really dramatic. The color is directly related to the stress and different thicknesses resulting from the molding process. This opens a wonderful discussion on how fast light travels through different materials (a great time to do the glass rod in a beaker of water trick and Snell's Law), then refractive index (an important optical property used in crime labs to tell where glass comes from in a crime scene). That leads to the discussion of birefringence (different RI sitting in different direction) which the treasured Shinya Inoue so graphically illustrated by cutting two long rectangles of wood: one WITH the grain and one AGAINST the grain (aobut 1" x 1" x 8"). I think there was a hole drilled across the short end so that the wood could be suspended from a chord, like a chime. Each block was then hit with a rubber mallet. They make very different sounds, depending on what the impulse wave encounters (with or against the grain). The analogy can then be made for light. By the way, of course, Shinya being Shinya, he stained and finished). There are a lot of follow-on experiments: making optical wedges with cellophane tape, optical additon and subtraction, etc. Also, if you have access to microscopes, there are baby cameras that can be place in lieu of the eyepiece. There are a whole raft of experiments on growing crystals under the microscope both between crossed polars and in normal brightfield, and, even if you don't do the Pol unit, the kids will get a big kick out them. I hope that this was helpful. Best regards, Barbara Foster, President Microscopy/Microscopy Education 7101 Royal Glen Trail, Suite A McKinney TX 75070 P: (972)924-5310 Skype: fostermme W: www.MicroscopyEducation.com MME is now scheduling customized, on-site courses through July 2008. Call us today for details That should get you st At 10:06 AM 2/14/2008, Mayandi Sivaguru wrote: Search the CONFOCAL archive at http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal Dear members of the list, We are planning to offer a one week summer program for 8th and 9th graders on Optical microscopy and imaging techniques. I kindly request your input on any available book or literature at this level with basic geometrical optics, optical path, image formation, contrasting techniques including polarization information and any available easy to understand tools and kits (especially for diffraction patterns) that could be used for the students to make mini projects. |
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In reply to this post by cromey
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We run a school outreach program. I'm at ACMM20 right
now
so can't check on what we have, but you might like to
contact
Tony Romeo ([hidden email]) who is
the
coordinator of the course. For diffraction, a very
good demo
is to use some sort of fine mesh (an EM grid is good) in
front
of a laser pointer. You can show the effects of
different mesh
sizes, and the same mesh with red and green
lasers.
For diffraction in the microscope, the Abbe Diffraction
Kit
simulation is available for free download on my
website
http://www.guycox.com/diffkit/diffkit.htm but
it might be
a bit too advanced for 8th / 9th grade (though a
schoolboy
-my son - did the coding).
Guy
Optical Imaging Techniques in Cell Biology From: Confocal Microscopy
List [mailto:[hidden email]] On Behalf Of Mayandi
Sivaguru Search the CONFOCAL archive at
http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal Dear members of the list, We are planning to offer a one week
summer program for 8th and 9th graders on “Optical microscopy and imaging
techniques”. I kindly request your input on any available book or literature at
this level with basic geometrical optics, optical path, image formation,
contrasting techniques including polarization information and any available easy
to understand tools and kits (especially for diffraction patterns) that could be
used for the students to make mini projects. Mayandi Sivaguru, PhD, PhD No virus found in this incoming message. No virus found in this outgoing message. |
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GeneMaverick |
Re: Microscopy techniques and tools for 8th and 9th graders
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In reply to this post by Mayandi Sivaguru
Search the CONFOCAL archive at
http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal Hello Sivaguru, I would like to suggest you to go through the site http://www.microscopy-uk.org.uk/primer/basics.htm http://www.microscopyu.com/ http://www.bio.uci.edu/academic/grad/Basic%20Training/Microscopy1_files/frame.htm http://science.howstuffworks.com/light-microscope1.htm In addition, I shall send some files to you by mail. Regards, Gene Maverick, Application Specialist Nikon Cell and Molecular Imaging Group. |
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George McNamara |
Re: Microscopy techniques and tools for 8th and 9th graders
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In reply to this post by Mayandi Sivaguru
Search the CONFOCAL archive at
http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
Hi Shiv, Edmund Optics sells various kits. Carolina Biological Supply (www.carolina.com ) sells lots of microscopy slide sets - hopefully a few will get the ADD crowd for focus on your session. You might also get some pointers from Ron Vale at http://www.microscopy4kids.org/ The Royal Microscopical Society put out 49 short (~120 pages) inexpensive (~$20 new) handbooks, see http://www.rms.org.uk/other-publications.shtml and www.amazon.com (contact the RMS for the list of titles, then amazon.com for any that the RMS does not have in stock). Titles included Introduction to Light Microscopy, Polarization Microscopy. You might also be able to get reprints (pdf?) from the RMS or Zeiss of a Proc RMS article in the Zeiss centenary issue on diffraction patterns in the back focal plane, and how to manipulate resolution by blocking parts of the pattern. RMS' InFocus (the successor of Proc RMS) may also be a good venue to publish your project in. George full disclosure: member RMS. At 10:21 AM 2/14/2008, you wrote: Search the CONFOCAL archive at http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal Dear members of the list, We are planning to offer a one week summer program for 8th and 9th graders on Optical microscopy and imaging techniques. I kindly request your input on any available book or literature at this level with basic geometrical optics, optical path, image formation, contrasting techniques including polarization information and any available easy to understand tools and kits (especially for diffraction patterns) that could be used for the students to make mini projects. George McNamara, Ph.D. University of Miami, Miller School of Medicine Image Core Miami, FL 33010 [hidden email] [hidden email] 305-243-8436 office http://home.earthlink.net/~pubspectra/ http://home.earthlink.net/~geomcnamara/ http://www.sylvester.org/research/SR_lab_analytical.asp?ana=desc (Analytical Imaging Core Facility) |
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