Microwave processor- Pelco Biowave

[Chloe van Oostende]

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Dear Microscopy specialists,
I’m seeking for some feedback on the use of the microwave Pelco Biowave from Ted Pella.
Using low power microwave energy, several papers promote the benefits of this machine allow to cut the time for fixation and the immunostaining by 10 to 60 times. The samples (can be culture cells, brain tissue, plant, other) integrity will be equal or better to conventional bench-based immunolabelling  technic.
I would like to buy it, but my users are not unanimously convinced that it works well on animal  tissue...
Any positive or negative feedbacks will be appreciated:
For how long have you used a Biowave with the ColdSpot?
Is it difficult to operate?
Is it difficult to optimize the workflow and find the  optimal settings?
Do you use it regularly?
Would you recommend it

Thank you very much in advance!
Best
Chloë

[Steve Ruzin-2]

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 Hi Chloe:  
 

 
We have extensive experience with microwave tissue processing and find that it helps significantly. The oven we use is the MRA BP111RS. It requires no “Cold Spot” which makes it a bit easier to use.  
 

 
You are welcome to contact me offline for more details.
 

 
Steve
 
 
 
 Steve Ruzin  
 [hidden email]
 
Biological Imaging Facility
 
University of California Berkeley
 
 
 
 

 
 

>  
> On Oct 17, 2018 at 6:21 AM,  <Chloë van Oostende-Triplet (mailto:[hidden email])>  wrote:
>  
>  
>  
>  *****  
> To join, leave or search the confocal microscopy listserv, go to:  
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy 
> Post images on http://www.imgur.com and include the link in your posting.  
> *****  
>
> Dear Microscopy specialists,  
> I’m seeking for some feedback on the use of the microwave Pelco Biowave from Ted Pella.  
> Using low power microwave energy, several papers promote the benefits of this machine allow to cut the time for fixation and the immunostaining by 10 to 60 times. The samples (can be culture cells, brain tissue, plant, other) integrity will be equal or better to conventional bench-based immunolabelling technic.  
> I would like to buy it, but my users are not unanimously convinced that it works well on animal tissue...  
> Any positive or negative feedbacks will be appreciated:  
> For how long have you used a Biowave with the ColdSpot?  
> Is it difficult to operate?  
> Is it difficult to optimize the workflow and find the optimal settings?  
> Do you use it regularly?  
> Would you recommend it  
>
> Thank you very much in advance!  
> Best  
> Chloë  
>

[Chris Guerin-2]

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Hi Chloë:

        Our core uses the BioWave. We bought it in 2012 and now process most of our samples in it although we still use the oven for polymerization of the resin. It speeds up processing in our lab greatly. Complex protocols such as the ROTO staining for volume EM drop from 24 hours to 90 minutes. It paid for itself in technicians salary saved in about 6 months. It has been reliable, easy to operate and is easy to program for standard protocols. It produces great results even with difficult samples like plant tissue. I’d recommend it highly.

Kind regards,

Chris Guérin
VIB Bioimaging Core Ghent

[Chloe van Oostende]

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Thanks Chris for your reply!
Do you process also fluorescing samples e.g. for super resolution
microscopy ?
Best
Chloë

Le jeu. 18 oct. 2018 à 06:22, Chris Guerin <[hidden email]>
a écrit :

> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> Post images on http://www.imgur.com and include the link in your posting.
> *****
>
> Hi Chloë:
>
>         Our core uses the BioWave. We bought it in 2012 and now process
> most of our samples in it although we still use the oven for polymerization
> of the resin. It speeds up processing in our lab greatly. Complex protocols
> such as the ROTO staining for volume EM drop from 24 hours to 90 minutes.
> It paid for itself in technicians salary saved in about 6 months. It has
> been reliable, easy to operate and is easy to program for standard
> protocols. It produces great results even with difficult samples like plant
> tissue. I’d recommend it highly.
>
> Kind regards,
>
> Chris Guérin
> VIB Bioimaging Core Ghent

--
Sent from 📱. I apologize for typos

Chloë van Oostende, PhD
Microscopy senior specialist - Facility Manager
Faculty of Medicine-Uottawa
451 smyth road
Ottawa, ON,
Canada