Mirrored glass slide?

[Gary Laevsky]

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Hi All,

Searched Google, Thorlabs, and Edmund.  Does anyone know where I can find a
mirrored glass slide?

Thanks

--
Best,

Gary Laevsky, Ph.D.
Director, Confocal Imaging Facility
Nikon Center of Excellence
Dept. of Molecular Biology
Washington Rd.
Princeton University
Princeton, New Jersey, 08544-1014
(O) 609 258 5432
(C) 508 507 1310

[Oshel, Philip Eugene]

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Gary,

How much, if any, does the EM lab at Princeton charge? You can make your own mirrored slides with metal evaporation in a vacuum evaporator, or in a sputter coater. 100% reflectance could be an issue, as that requires a thick coat of metal (time depends on metal and etc.), but you could work out the coating rate and produce mirrors that reflect X% and transmit 100-X%, “X” as needed (like 50/50 or 20/80, etc.).

Phil
-------------
Philip Oshel    
Imaging Facility Director
Biology Department
1304 Biosciences
1455 Calumet Ct.
Central Michigan University
Mt. Pleasant, MI 48859
989 774-3576 office
989 774-7567 lab




-----Original Message-----
From: Confocal Microscopy List <[hidden email]> on behalf of Gary Laevsky <[hidden email]>
Reply-To: Confocal Microscopy List <[hidden email]>
Date: Wednesday,  26July, 2017 at 10:36
To: "[hidden email]" <[hidden email]>
Subject: Mirrored glass slide?

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*****

Hi All,

Searched Google, Thorlabs, and Edmund.  Does anyone know where I can find a
mirrored glass slide?

Thanks

--
Best,

Gary Laevsky, Ph.D.
Director, Confocal Imaging Facility
Nikon Center of Excellence
Dept. of Molecular Biology
Washington Rd.
Princeton University
Princeton, New Jersey, 08544-1014
(O) 609 258 5432
(C) 508 507 1310

[JOEL B. SHEFFIELD]

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Are you looking for a front surface mirror, also called "first surface
mirror?  Ore are you concerned about the dimensions.  It is not that
difficult to cut glass.  Hee's a listing from Edmunds Optical.

https://www.edmundoptics.com/search/?#stq=front+surface+mirror&stp=1



Joel B. Sheffield, Ph.D
Department of Biology
Temple University
Philadelphia, PA 19122
Voice: 215 204 8839
e-mail: [hidden email]
URL:  *https://bio.cst.temple.edu/~jbs/ <https://bio.cst.temple.edu/~jbs/>
<http://tinyurl.com/khbouft>*

On Wed, Jul 26, 2017 at 10:36 AM, Gary Laevsky <[hidden email]>
wrote:

> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> Post images on http://www.imgur.com and include the link in your posting.
> *****
>
> Hi All,
>
> Searched Google, Thorlabs, and Edmund.  Does anyone know where I can find a
> mirrored glass slide?
>
> Thanks
>
> --
> Best,
>
> Gary Laevsky, Ph.D.
> Director, Confocal Imaging Facility
> Nikon Center of Excellence
> Dept. of Molecular Biology
> Washington Rd.
> Princeton University
> Princeton, New Jersey, 08544-1014
> (O) 609 258 5432
> (C) 508 507 1310
>

[Glenn Merrill-Skoloff]

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Chroma will likely make one for you to spec.

-  Glenn

Glenn Merrill-Skoloff
Laboratory Manager
Director, Intravital Microscopy Core

Beth Israel Deaconess Medical Center
Division of Hemostasis and Thrombosis
3 Blackfan Circle - CLS 942
[phone]
[hidden email]

From: Confocal Microscopy List <[hidden email]<mailto:[hidden email]>> on behalf of Gary Laevsky <[hidden email]<mailto:[hidden email]>>
Reply-To: Confocal Microscopy List <[hidden email]<mailto:[hidden email]>>
Date: Wednesday, July 26, 2017 at 10:36 AM
To: "[hidden email]<mailto:[hidden email]>" <[hidden email]<mailto:[hidden email]>>
Subject: Mirrored glass slide?

*****
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Post images on https://urldefense.proofpoint.com/v2/url?u=http-3A__www.imgur.com&d=DwIBaQ&c=WknmpdNpvrlj2B5K1aWVqL1SOiF30547pqSuOmtwXTQ&r=42NmABDnL7YbxR4oFPmDN-5XK3Kw7RY6XnrLkSD93J4&m=nhItnBCnls8yjtywleFO8ftZCbFTfYOjC36VoXw3PlM&s=lSIc13MZi5l42fr66XT4zfhja4s8CJQkTuODr_PyY_g&e=  and include the link in your posting.
*****

Hi All,

Searched Google, Thorlabs, and Edmund.  Does anyone know where I can find a
mirrored glass slide?

Thanks

--
Best,

Gary Laevsky, Ph.D.
Director, Confocal Imaging Facility
Nikon Center of Excellence
Dept. of Molecular Biology
Washington Rd.
Princeton University
Princeton, New Jersey, 08544-1014
(O) 609 258 5432
(C) 508 507 1310


________________________________

This message is intended for the use of the person(s) to whom it may be addressed. It may contain information that is privileged, confidential, or otherwise protected from disclosure under applicable law. If you are not the intended recipient, any dissemination, distribution, copying, or use of this information is prohibited. If you have received this message in error, please permanently delete it and immediately notify the sender. Thank you.

[Zdenek Svindrych-2]

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Hi Gary,
long time ago I used to make my own with a standard silvering solution
(silver nitrate and some reducing agent), but those were not very good as
first surface mirrors. It worked much better to silver a coverslip and glue
it to a glass slide with the silver 'inside'. Still it did not last very
long... Protected Al is much better, of course.


Also, neither glass slides nor coverslips are flat, so it is useless for
anything else than reflecting a laser beam back to a microscope objective...

When I need a cheap mirror for experiments, I look into some old data
projector (dielectric mirrors and filters) or a flatbed scanner (very good
first surface metallic mirrors), though the dimensions are quite different
from a glass slide (usually much thicker and narrower).

Best, zdenek


--
Zdenek Svindrych, Ph.D.
W.M. Keck Center for Cellular Imaging (PLSB 003)
Department of Biology,University of Virginia
409 McCormick Rd, Charlottesville, VA-22904
http://www.kcci.virginia.edu/
tel: [phone]

---------- Původní e-mail ----------
Od: Gary Laevsky <[hidden email]>
Komu: [hidden email]
Datum: 26. 7. 2017 10:37:12
Předmět: Mirrored glass slide?
"*****
To join, leave or search the confocal microscopy listserv, go to:
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Post images on http://www.imgur.com and include the link in your posting.
*****

Hi All,

Searched Google, Thorlabs, and Edmund. Does anyone know where I can find a
mirrored glass slide?

Thanks

--
Best,

Gary Laevsky, Ph.D.
Director, Confocal Imaging Facility
Nikon Center of Excellence
Dept. of Molecular Biology
Washington Rd.
Princeton University
Princeton, New Jersey, 08544-1014
(O) 609 258 5432
(C) 508 507 1310
"

[Gary Laevsky]

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*****

Hi All,

Thanks for the suggestions.  All set.  Doesn't need to be crazy precise.

https://opticalmirror.com/

This place lets you order them in any size you want. Slide dimensions
(3"x1"x1mm) is $20 for 1, less if you order more.

On Wed, Jul 26, 2017 at 10:50 AM, Merrill-Skoloff,Glenn <
[hidden email]> wrote:

> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> Post images on http://www.imgur.com and include the link in your posting.
> *****
>
> Chroma will likely make one for you to spec.
>
> -  Glenn
>
> Glenn Merrill-Skoloff
> Laboratory Manager
> Director, Intravital Microscopy Core
>
> Beth Israel Deaconess Medical Center
> Division of Hemostasis and Thrombosis
> 3 Blackfan Circle - CLS 942
> [phone]
> [hidden email]
>
> From: Confocal Microscopy List <[hidden email]<mailto:
> [hidden email]>> on behalf of Gary Laevsky <
> [hidden email]<mailto:[hidden email]>>
> Reply-To: Confocal Microscopy List <[hidden email]
> <mailto:[hidden email]>>
> Date: Wednesday, July 26, 2017 at 10:36 AM
> To: "[hidden email]<mailto:CONFOCALMICROSCOPY@
> LISTS.UMN.EDU>" <[hidden email]<mailto:
> [hidden email]>>
> Subject: Mirrored glass slide?
>
> *****
> To join, leave or search the confocal microscopy listserv, go to:
> https://urldefense.proofpoint.com/v2/url?u=http-3A__lists.
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> Post images on https://urldefense.proofpoint.com/v2/url?u=http-3A__www.
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> lSIc13MZi5l42fr66XT4zfhja4s8CJQkTuODr_PyY_g&e=  and include the link in
> your posting.
> *****
>
> Hi All,
>
> Searched Google, Thorlabs, and Edmund.  Does anyone know where I can find a
> mirrored glass slide?
>
> Thanks
>
> --
> Best,
>
> Gary Laevsky, Ph.D.
> Director, Confocal Imaging Facility
> Nikon Center of Excellence
> Dept. of Molecular Biology
> Washington Rd.
> Princeton University
> Princeton, New Jersey, 08544-1014
> (O) 609 258 5432
> (C) 508 507 1310
>
>
> ________________________________
>
> This message is intended for the use of the person(s) to whom it may be
> addressed. It may contain information that is privileged, confidential, or
> otherwise protected from disclosure under applicable law. If you are not
> the intended recipient, any dissemination, distribution, copying, or use of
> this information is prohibited. If you have received this message in error,
> please permanently delete it and immediately notify the sender. Thank you.
>

--
Best,

Gary Laevsky, Ph.D.
Director, Confocal Imaging Facility
Nikon Center of Excellence
Dept. of Molecular Biology
Washington Rd.
Princeton University
Princeton, New Jersey, 08544-1014
(O) 609 258 5432
(C) 508 507 1310

[Guy Hagen]

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Hello:

Leica can supply a mirrored coverslip mounted in a 1x3 inch carrier. In
this way, you would be imaging the mirror surface through the coverslip and
thereby getting the best optical performance. Leica uses this to align the
pinhole in their confocal systems. However I would remind everyone that
this is not a good way to characterize the optical sectioning ability of a
microscope - a thin fluorescent film should be used instead.

best wishes,
Guy Hagen

On Wed, Jul 26, 2017 at 10:08 AM, Gary Laevsky <[hidden email]>
wrote:

> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> Post images on http://www.imgur.com and include the link in your posting.
> *****
>
> Hi All,
>
> Thanks for the suggestions.  All set.  Doesn't need to be crazy precise.
>
> https://opticalmirror.com/
>
> This place lets you order them in any size you want. Slide dimensions
> (3"x1"x1mm) is $20 for 1, less if you order more.
>
> On Wed, Jul 26, 2017 at 10:50 AM, Merrill-Skoloff,Glenn <
> [hidden email]> wrote:
>
> > *****
> > To join, leave or search the confocal microscopy listserv, go to:
> > http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> > Post images on http://www.imgur.com and include the link in your
> posting.
> > *****
> >
> > Chroma will likely make one for you to spec.
> >
> > -  Glenn
> >
> > Glenn Merrill-Skoloff
> > Laboratory Manager
> > Director, Intravital Microscopy Core
> >
> > Beth Israel Deaconess Medical Center
> > Division of Hemostasis and Thrombosis
> > 3 Blackfan Circle - CLS 942
> > [phone]
> > [hidden email]
> >
> > From: Confocal Microscopy List <[hidden email]<mailto:
> > [hidden email]>> on behalf of Gary Laevsky <
> > [hidden email]<mailto:[hidden email]>>
> > Reply-To: Confocal Microscopy List <[hidden email]
> > <mailto:[hidden email]>>
> > Date: Wednesday, July 26, 2017 at 10:36 AM
> > To: "[hidden email]<mailto:CONFOCALMICROSCOPY@
> > LISTS.UMN.EDU>" <[hidden email]<mailto:
> > [hidden email]>>
> > Subject: Mirrored glass slide?
> >
> > *****
> > To join, leave or search the confocal microscopy listserv, go to:
> > https://urldefense.proofpoint.com/v2/url?u=http-3A__lists.
> > umn.edu_cgi-2Dbin_wa-3FA0-3Dconfocalmicroscopy&d=DwIBaQ&c=
> > WknmpdNpvrlj2B5K1aWVqL1SOiF30547pqSuOmtwXTQ&r=42NmABDnL7YbxR4oFPmDN-
> > 5XK3Kw7RY6XnrLkSD93J4&m=nhItnBCnls8yjtywleFO8ftZCbFTfYOjC36VoXw3PlM&s=
> > OchtbG5fTTt3BS3X3Fnnbrhjv_ic0lHGfp-ZstB7kl4&e=
> > Post images on https://urldefense.proofpoint.com/v2/url?u=http-3A__www.
> > imgur.com&d=DwIBaQ&c=WknmpdNpvrlj2B5K1aWVqL1SOiF30547pqSuOmtwXTQ&r=
> > 42NmABDnL7YbxR4oFPmDN-5XK3Kw7RY6XnrLkSD93J4&m=
> > nhItnBCnls8yjtywleFO8ftZCbFTfYOjC36VoXw3PlM&s=
> > lSIc13MZi5l42fr66XT4zfhja4s8CJQkTuODr_PyY_g&e=  and include the link in
> > your posting.
> > *****
> >
> > Hi All,
> >
> > Searched Google, Thorlabs, and Edmund.  Does anyone know where I can
> find a
> > mirrored glass slide?
> >
> > Thanks
> >
> > --
> > Best,
> >
> > Gary Laevsky, Ph.D.
> > Director, Confocal Imaging Facility
> > Nikon Center of Excellence
> > Dept. of Molecular Biology
> > Washington Rd.
> > Princeton University
> > Princeton, New Jersey, 08544-1014
> > (O) 609 258 5432
> > (C) 508 507 1310
> >
> >
> > ________________________________
> >
> > This message is intended for the use of the person(s) to whom it may be
> > addressed. It may contain information that is privileged, confidential,
> or
> > otherwise protected from disclosure under applicable law. If you are not
> > the intended recipient, any dissemination, distribution, copying, or use
> of
> > this information is prohibited. If you have received this message in
> error,
> > please permanently delete it and immediately notify the sender. Thank
> you.
> >
>
>
>
> --
> Best,
>
> Gary Laevsky, Ph.D.
> Director, Confocal Imaging Facility
> Nikon Center of Excellence
> Dept. of Molecular Biology
> Washington Rd.
> Princeton University
> Princeton, New Jersey, 08544-1014
> (O) 609 258 5432
> (C) 508 507 1310
>