Modelling objective lenses for microscope design

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Alfred Millett-Sikking Alfred Millett-Sikking
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Modelling objective lenses for microscope design

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Dear all,

I'm trying to model a new microscope design in Zemax that uses multiple
objective lenses, but can't access any detailed specifications on lens
design. Modelling them as ideal components seems too simplistic...

Has anyone found a good solution to this problem?

Any experience, help, tips or advice much appreciated,

Thanks,

Alfred.
John Oreopoulos John Oreopoulos
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Re: Modelling objective lenses for microscope design

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Dear Alfred,

There is an excellent book available from SPIE that contains several "recipes" for modelling objective lenses in Zemax, including a full Nikon TIRF objective:

http://spie.org/Publications/Book/855480



Cheers,

John Oreopoulos
Staff Scientist
Spectral Applied Research Inc.
A Division of Andor Technology
Richmond Hill, Ontario
Canada
www.spectral.ca



On 2015-09-29, at 7:15 PM, Alfred Millett-Sikking wrote:

> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> Post images on http://www.imgur.com and include the link in your posting.
> *****
>
> Dear all,
>
> I'm trying to model a new microscope design in Zemax that uses multiple
> objective lenses, but can't access any detailed specifications on lens
> design. Modelling them as ideal components seems too simplistic...
>
> Has anyone found a good solution to this problem?
>
> Any experience, help, tips or advice much appreciated,
>
> Thanks,
>
> Alfred.
Miklos Erdelyi Miklos Erdelyi
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Re: Modelling objective lenses for microscope design

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*****

Dear ALfred,
Try to find the patent of the applied objective. The patent gives you all
the relevant info (thickness, Abbe number, refractive index, rad. etc.).
Unfortunately, manufacturers do not mark the patent number on the
objectives, so you have to find it based on its NA, magnification, WD, cover
glass correction, manufacturer...  
Cheers,
Miklos
Mark Cannell-2 Mark Cannell-2
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Re: Modelling objective lenses for microscope design

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Hi Alfred

I have used Zemax for objective lens design and may be able to help. You can contact me directly at my email below.

Cheers Mark

On 30/09/2015, at 12:15 am, Alfred Millett-Sikking <[hidden email]> wrote:

> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> Post images on http://www.imgur.com and include the link in your posting.
> *****
>
> Dear all,
>
> I'm trying to model a new microscope design in Zemax that uses multiple
> objective lenses, but can't access any detailed specifications on lens
> design. Modelling them as ideal components seems too simplistic...
>
> Has anyone found a good solution to this problem?
>
> Any experience, help, tips or advice much appreciated,
>
> Thanks,
>
> Alfred.

Mark  B. Cannell Ph.D. FRSNZ
Professor of Cardiac Cell Biology
School of Physiology &  Pharmacology
Faculty of Biomedical Sciences
University of Bristol
Bristol
BS8 1TD UK

[hidden email]
lechristophe lechristophe
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Re: Modelling objective lenses for microscope design

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*****
To join, leave or search the confocal microscopy listserv, go to:
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
Post images on http://www.imgur.com and include the link in your posting.
*****

Hi Alfred,

You can have a look at a recent preprint, where the authors have modeled
several objectives and made the Zemax files available (I'm not a specialist
so I'm not sure that it is exactly what you look for, but it looks quite
close):

http://arxiv.org/abs/1507.04037

*Comparative analysis of imaging configurations and objectives for Fourier
microscopy*
*Jonathan A. Kurvits, Mingming Jiang, Rashid Zia*
(Submitted on 14 Jul 2015)

Fourier microscopy is becoming an increasingly important tool for the
analysis of optical nanostructures and quantum emitters. However, achieving
quantitative Fourier space measurements requires a thorough understanding
of the impact of aberrations introduced by optical microscopes, which have
been optimized for conventional real-space imaging. Here, we present a
detailed framework for analyzing the performance of microscope objectives
for several common Fourier imaging configurations. To this end, we model
objectives from Nikon, Olympus, and Zeiss using parameters that were
inferred from patent literature and confirmed, where possible, by physical
disassembly. We then examine the aberrations most relevant to Fourier
microscopy, including the alignment tolerances of apodization factors for
different objective classes, the effect of magnification on the modulation
transfer function, and vignetting-induced reductions of the effective
numerical aperture for wide-field measurements. Based on this analysis, we
identify an optimal objective class and imaging configuration for Fourier
microscopy. In addition, as a resource for future studies, the Zemax files
for the objectives and setups used in this analysis have been made publicly
available.

------------

Best Regards,

Christophe


Christophe Leterrier
Researcher
Axonal Domains Architecture Team
CRN2M CNRS UMR 7286
Aix Marseille University, France







On 30 September 2015 at 01:15, Alfred Millett-Sikking <[hidden email]>
wrote:

> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> Post images on http://www.imgur.com and include the link in your posting.
> *****
>
> Dear all,
>
> I'm trying to model a new microscope design in Zemax that uses multiple
> objective lenses, but can't access any detailed specifications on lens
> design. Modelling them as ideal components seems too simplistic...
>
> Has anyone found a good solution to this problem?
>
> Any experience, help, tips or advice much appreciated,
>
> Thanks,
>
> Alfred.
>
lechristophe lechristophe
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Re: Modelling objective lenses for microscope design

*****
To join, leave or search the confocal microscopy listserv, go to:
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
Post images on http://www.imgur.com and include the link in your posting.
*****

Here is the link the the Zemax files:

http://figshare.com/articles/Zemax_optical_design_files_of_microscope_objectives_tube_lenses_and_Fourier_imaging_setups/1481270

Christophe

On 30 September 2015 at 09:39, Christophe Leterrier <
[hidden email]> wrote:

> Hi Alfred,
>
> You can have a look at a recent preprint, where the authors have modeled
> several objectives and made the Zemax files available (I'm not a specialist
> so I'm not sure that it is exactly what you look for, but it looks quite
> close):
>
> http://arxiv.org/abs/1507.04037
>
> *Comparative analysis of imaging configurations and objectives for Fourier
> microscopy*
> *Jonathan A. Kurvits, Mingming Jiang, Rashid Zia*
> (Submitted on 14 Jul 2015)
>
> Fourier microscopy is becoming an increasingly important tool for the
> analysis of optical nanostructures and quantum emitters. However, achieving
> quantitative Fourier space measurements requires a thorough understanding
> of the impact of aberrations introduced by optical microscopes, which have
> been optimized for conventional real-space imaging. Here, we present a
> detailed framework for analyzing the performance of microscope objectives
> for several common Fourier imaging configurations. To this end, we model
> objectives from Nikon, Olympus, and Zeiss using parameters that were
> inferred from patent literature and confirmed, where possible, by physical
> disassembly. We then examine the aberrations most relevant to Fourier
> microscopy, including the alignment tolerances of apodization factors for
> different objective classes, the effect of magnification on the modulation
> transfer function, and vignetting-induced reductions of the effective
> numerical aperture for wide-field measurements. Based on this analysis, we
> identify an optimal objective class and imaging configuration for Fourier
> microscopy. In addition, as a resource for future studies, the Zemax files
> for the objectives and setups used in this analysis have been made publicly
> available.
>
> ------------
>
> Best Regards,
>
> Christophe
>
>
> Christophe Leterrier
> Researcher
> Axonal Domains Architecture Team
> CRN2M CNRS UMR 7286
> Aix Marseille University, France
>
>
>
>
>
>
>
> On 30 September 2015 at 01:15, Alfred Millett-Sikking <[hidden email]
> > wrote:
>
>> *****
>> To join, leave or search the confocal microscopy listserv, go to:
>> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
>> Post images on http://www.imgur.com and include the link in your posting.
>> *****
>>
>> Dear all,
>>
>> I'm trying to model a new microscope design in Zemax that uses multiple
>> objective lenses, but can't access any detailed specifications on lens
>> design. Modelling them as ideal components seems too simplistic...
>>
>> Has anyone found a good solution to this problem?
>>
>> Any experience, help, tips or advice much appreciated,
>>
>> Thanks,
>>
>> Alfred.
>>
>
>
lechristophe lechristophe
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Re: Modelling objective lenses for microscope design

*****
To join, leave or search the confocal microscopy listserv, go to:
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
Post images on http://www.imgur.com and include the link in your posting.
*****

and last but not least, a link to the published article (Optical Society of
America A, 09/15/2015, scroll down the page to find the updated PDF) :
https://www.osapublishing.org/josaa/upcoming.cfm

Christophe

On 30 September 2015 at 09:44, Christophe Leterrier <
[hidden email]> wrote:

> Here is the link the the Zemax files:
>
>
> http://figshare.com/articles/Zemax_optical_design_files_of_microscope_objectives_tube_lenses_and_Fourier_imaging_setups/1481270
>
> Christophe
>
> On 30 September 2015 at 09:39, Christophe Leterrier <
> [hidden email]> wrote:
>
>> Hi Alfred,
>>
>> You can have a look at a recent preprint, where the authors have modeled
>> several objectives and made the Zemax files available (I'm not a specialist
>> so I'm not sure that it is exactly what you look for, but it looks quite
>> close):
>>
>> http://arxiv.org/abs/1507.04037
>>
>> *Comparative analysis of imaging configurations and objectives for
>> Fourier microscopy*
>> *Jonathan A. Kurvits, Mingming Jiang, Rashid Zia*
>> (Submitted on 14 Jul 2015)
>>
>> Fourier microscopy is becoming an increasingly important tool for the
>> analysis of optical nanostructures and quantum emitters. However, achieving
>> quantitative Fourier space measurements requires a thorough understanding
>> of the impact of aberrations introduced by optical microscopes, which have
>> been optimized for conventional real-space imaging. Here, we present a
>> detailed framework for analyzing the performance of microscope objectives
>> for several common Fourier imaging configurations. To this end, we model
>> objectives from Nikon, Olympus, and Zeiss using parameters that were
>> inferred from patent literature and confirmed, where possible, by physical
>> disassembly. We then examine the aberrations most relevant to Fourier
>> microscopy, including the alignment tolerances of apodization factors for
>> different objective classes, the effect of magnification on the modulation
>> transfer function, and vignetting-induced reductions of the effective
>> numerical aperture for wide-field measurements. Based on this analysis, we
>> identify an optimal objective class and imaging configuration for Fourier
>> microscopy. In addition, as a resource for future studies, the Zemax files
>> for the objectives and setups used in this analysis have been made publicly
>> available.
>>
>> ------------
>>
>> Best Regards,
>>
>> Christophe
>>
>>
>> Christophe Leterrier
>> Researcher
>> Axonal Domains Architecture Team
>> CRN2M CNRS UMR 7286
>> Aix Marseille University, France
>>
>>
>>
>>
>>
>>
>>
>> On 30 September 2015 at 01:15, Alfred Millett-Sikking <
>> [hidden email]> wrote:
>>
>>> *****
>>> To join, leave or search the confocal microscopy listserv, go to:
>>> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
>>> Post images on http://www.imgur.com and include the link in your
>>> posting.
>>> *****
>>>
>>> Dear all,
>>>
>>> I'm trying to model a new microscope design in Zemax that uses multiple
>>> objective lenses, but can't access any detailed specifications on lens
>>> design. Modelling them as ideal components seems too simplistic...
>>>
>>> Has anyone found a good solution to this problem?
>>>
>>> Any experience, help, tips or advice much appreciated,
>>>
>>> Thanks,
>>>
>>> Alfred.
>>>
>>
>>
>
Alfred Millett-Sikking Alfred Millett-Sikking
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Re: Modelling objective lenses for microscope design

*****
To join, leave or search the confocal microscopy listserv, go to:
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
Post images on http://www.imgur.com and include the link in your posting.
*****

Dear all,

Just to say thank you for your guidance and references on this issue, it
has been most useful and is much appreciated.

Alfred.

On 30 September 2015 at 00:49, Christophe Leterrier <
[hidden email]> wrote:

> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> Post images on http://www.imgur.com and include the link in your posting.
> *****
>
> and last but not least, a link to the published article (Optical Society of
> America A, 09/15/2015, scroll down the page to find the updated PDF) :
> https://www.osapublishing.org/josaa/upcoming.cfm
>
> Christophe
>
> On 30 September 2015 at 09:44, Christophe Leterrier <
> [hidden email]> wrote:
>
> > Here is the link the the Zemax files:
> >
> >
> >
> http://figshare.com/articles/Zemax_optical_design_files_of_microscope_objectives_tube_lenses_and_Fourier_imaging_setups/1481270
> >
> > Christophe
> >
> > On 30 September 2015 at 09:39, Christophe Leterrier <
> > [hidden email]> wrote:
> >
> >> Hi Alfred,
> >>
> >> You can have a look at a recent preprint, where the authors have modeled
> >> several objectives and made the Zemax files available (I'm not a
> specialist
> >> so I'm not sure that it is exactly what you look for, but it looks quite
> >> close):
> >>
> >> http://arxiv.org/abs/1507.04037
> >>
> >> *Comparative analysis of imaging configurations and objectives for
> >> Fourier microscopy*
> >> *Jonathan A. Kurvits, Mingming Jiang, Rashid Zia*
> >> (Submitted on 14 Jul 2015)
> >>
> >> Fourier microscopy is becoming an increasingly important tool for the
> >> analysis of optical nanostructures and quantum emitters. However,
> achieving
> >> quantitative Fourier space measurements requires a thorough
> understanding
> >> of the impact of aberrations introduced by optical microscopes, which
> have
> >> been optimized for conventional real-space imaging. Here, we present a
> >> detailed framework for analyzing the performance of microscope
> objectives
> >> for several common Fourier imaging configurations. To this end, we model
> >> objectives from Nikon, Olympus, and Zeiss using parameters that were
> >> inferred from patent literature and confirmed, where possible, by
> physical
> >> disassembly. We then examine the aberrations most relevant to Fourier
> >> microscopy, including the alignment tolerances of apodization factors
> for
> >> different objective classes, the effect of magnification on the
> modulation
> >> transfer function, and vignetting-induced reductions of the effective
> >> numerical aperture for wide-field measurements. Based on this analysis,
> we
> >> identify an optimal objective class and imaging configuration for
> Fourier
> >> microscopy. In addition, as a resource for future studies, the Zemax
> files
> >> for the objectives and setups used in this analysis have been made
> publicly
> >> available.
> >>
> >> ------------
> >>
> >> Best Regards,
> >>
> >> Christophe
> >>
> >>
> >> Christophe Leterrier
> >> Researcher
> >> Axonal Domains Architecture Team
> >> CRN2M CNRS UMR 7286
> >> Aix Marseille University, France
> >>
> >>
> >>
> >>
> >>
> >>
> >>
> >> On 30 September 2015 at 01:15, Alfred Millett-Sikking <
> >> [hidden email]> wrote:
> >>
> >>> *****
> >>> To join, leave or search the confocal microscopy listserv, go to:
> >>> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> >>> Post images on http://www.imgur.com and include the link in your
> >>> posting.
> >>> *****
> >>>
> >>> Dear all,
> >>>
> >>> I'm trying to model a new microscope design in Zemax that uses multiple
> >>> objective lenses, but can't access any detailed specifications on lens
> >>> design. Modelling them as ideal components seems too simplistic...
> >>>
> >>> Has anyone found a good solution to this problem?
> >>>
> >>> Any experience, help, tips or advice much appreciated,
> >>>
> >>> Thanks,
> >>>
> >>> Alfred.
> >>>
> >>
> >>
> >
>