Mosquitoes, ticks, other critters (brine shrimp mentioned previously) ... Re: Thick, low cost fluorescent or auto-fluorescent slides / samples?
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George McNamara |
Mosquitoes, ticks, other critters (brine shrimp mentioned previously) ... Re: Thick, low cost fluorescent or auto-fluorescent slides / samples?
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*****
To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy Post images on http://www.imgur.com and include the link in your posting. ***** * Mosquitoes, ticks, other critters (brine shrimp mentioned previously). * brightest white T-shirt (probably just need a micro piece). Sadly the Constallation microspheres have been discontinued: https://www.thermofisher.com/us/en/home/references/molecular-probes-the-handbook/ultrasensitive-detection-technology/microspheres.html Fluorescent Microspheres for Educational Purposes Constellation microspheres for imaging (C14837 <https://www.thermofisher.com/order/catalog/en/US/adirect/lt?cmd=catProductDetail&entryPoint=adirect&productID=C14837&messageType=catProductDetail>) can be used to demonstrate hands-on techniques with a fluorescence microscope. Constellation microspheres consist of a selected mixture of beads in assorted sizes and colors () that can be used to practice adjusting the focus and switching filters on a fluorescence microscope. These microspheres are stable at room temperature, so they can be conveniently stored. The web link leads to: This product (Catalog number C14837) has been discontinued. Please contactCustomer Service <https://www.thermofisher.com/us/en/home/technical-resources/contact-us.C14837.html?supportType=SL>for possible alternatives. so I suggest you ask ThermoFidher/LifeTech/Invitrogen/Molecular Probes for their current equivalent. My understanding is Constellation was made from the reject lots and leftovers of all of Mol Probes microspheres. Mount in some viscous solution, such as glycerol (something you know or can look up the refractive index; thickness: whatever you want: I would probably put a big drop in a mattek 35mm imaging dish and seal a coverglass on top ... on my "to do list" if/when I get a microscopy job). Acknowledgement: Constallation beads were a lot of fun on a Zeiss TIRF3 microscope demo -- widefield levitation vs TIRF "appear and disappear" -- I cohosted with Justin Price at University of Miami several years ago. My thanks to Kevin Johnson and Katherine Schafer-Hales and others at Zeiss for enabling the demo. George On 1/30/2017 9:17 AM, WAINWRIGHT James wrote: > ***** > To join, leave or search the confocal microscopy listserv, go to: > http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy > Post images onhttp://www.imgur.com and include the link in your posting. > ***** > > Thanks Gary - I'll give them a try. > > Yes - I'm after something with structure, rather than the Chroma plastic slides. > > James > > > > -----Original Message----- > From: Confocal Microscopy List [mailto:[hidden email]] On Behalf Of Gary Laevsky > Sent: 30 January 2017 14:11 > To:[hidden email] > Subject: Re: Hi James, ask Chroma for a box, or a case, of their fluorescent plastic slides ... Re: Thick, low cost fluorescent or auto-fluorescent slides / samples? > > EXTERNAL EMAIL > > ATTACHMENT ADVISORY > > ***** > To join, leave or search the confocal microscopy listserv, go to: > http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy > Post images onhttp://www.imgur.com and include the link in your posting. > ***** > > Brine shrimp? > > On Mon, Jan 30, 2017 at 9:03 AM, John Oreopoulos <[hidden email]> wrote: > >> ***** >> To join, leave or search the confocal microscopy listserv, go to: >> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy >> Post images onhttp://www.imgur.com and include the link in your posting. >> ***** >> >> I think he's looking for something with 3D structure, George, rather >> than just a fluorescent slab. >> >> John Oreopoulos >> >>> On Jan 30, 2017, at 8:43 AM, George McNamara >>> <[hidden email]> >> wrote: >>> ***** >>> To join, leave or search the confocal microscopy listserv, go to: >>> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy >>> Post images onhttp://www.imgur.com and include the link in your >> posting. >>> ***** >>> >>> Hi James, >>> >>> ask Chroma for a box, or a case, of their fluorescent plastic slides >> (you should give them your shipping number to send to you). I believe >> 1 mm thick (maybe more). >>> You can also visit their booth at the next trade show (Biophysics?). >>> >>> >>> sincerely, >>> >>> George >>> >>> >>>> On 1/30/2017 7:09 AM, WAINWRIGHT James wrote: >>>> ***** >>>> To join, leave or search the confocal microscopy listserv, go to: >>>> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy >>>> Post images onhttp://www.imgur.com and include the link in your >> posting. >>>> ***** >>>> >>>> Dear mailing list, >>>> >>>> I'm trying to find some low cost prepared slides that meet the >> following criteria: >>>> 1) Fluorescent, or auto-fluorescent with UV/VIS/NIR. >>>> >>>> 2) Quite thick - at least 100 µm, ideally 200 µm or more. >>>> >>>> 3) Low cost - i.e. ~£10 per slide. Cheaper = better. >>>> >>>> The most success I've had so far is with pollen grain prepared >>>> slides - >> these are wonderfully bright under fluorescence, detailed, low cost, >> but not very thick (typically up to 50 µm). Plant cross-sections such >> as Convallaria are also great, but again, tend to only be 10 µm or so thick. >>>> Many thanks for any ideas or suppliers you can provide, on or off-list. >>>> >>>> James >>>> >>>> >>>> >>>> ___________________________________________________________________ >>>> ________This >> e-mail is confidential and is for the addressee only. Please refer to >> www.oxinst.com/email-statement for regulatory information. >>> -- >>> >>> >>> George McNamara, PhD >>> Houston, TX 77054 >>> [hidden email] >>> https://www.linkedin.com/in/georgemcnamara >>> https://works.bepress.com/gmcnamara/75/ >>> http://www.ncbi.nlm.nih.gov/myncbi/browse/collection/44962650 > > -- > Best, > > Gary Laevsky, Ph.D. > Director, Confocal Imaging Facility > Nikon Center of Excellence > Dept. of Molecular Biology > Washington Rd. > Princeton University > Princeton, New Jersey, 08544-1014 > (O) 609 258 5432 > (C) 508 507 1310 > > > +++Scanned for Viruses by ForcePoint+++ -- George McNamara, PhD Houston, TX 77054 [hidden email] https://www.linkedin.com/in/georgemcnamara https://works.bepress.com/gmcnamara/75/ http://www.ncbi.nlm.nih.gov/myncbi/browse/collection/44962650 |
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Rosemary.White |
Re: Mosquitoes, ticks, other critters (brine shrimp mentioned previously) ... Re: Thick, low cost fluorescent or auto-fluorescent slides / samples?
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To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy Post images on http://www.imgur.com and include the link in your posting. ***** And then, of course, there is wood, though you’d probably have to make your own sections on a sliding microtome if you wanted them reasonably thick – the prepared slides are probably less than 20 microns thick. A colleague has just been looking at some wood slices about 100 or so microns thick, from stored pieces of branch fixed in formaldehyde (FAA I think). To soften for sectioning – rinse thoroughly, then a few minutes in the microwave. A wooden drinks coaster might work if you are imaging on the confocal. We used to look at a polished piece of on the confocal as a demo for school students. cheers, Rosemary Dr Rosemary White CSIRO Black Mountain GPO Box 1700 Canberra, ACT 2601 Australia Adjunct Prof, EH Graham Centre, CSU & Research School of Biology, ANU T 61 2 6246 5475 E [hidden email] On 31/1/17, 3:54 am, "Confocal Microscopy List on behalf of George McNamara" <[hidden email] on behalf of [hidden email]> wrote: ***** To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy Post images on http://www.imgur.com and include the link in your posting. ***** * Mosquitoes, ticks, other critters (brine shrimp mentioned previously). * brightest white T-shirt (probably just need a micro piece). Sadly the Constallation microspheres have been discontinued: https://www.thermofisher.com/us/en/home/references/molecular-probes-the-handbook/ultrasensitive-detection-technology/microspheres.html Fluorescent Microspheres for Educational Purposes Constellation microspheres for imaging (C14837 <https://www.thermofisher.com/order/catalog/en/US/adirect/lt?cmd=catProductDetail&entryPoint=adirect&productID=C14837&messageType=catProductDetail>) can be used to demonstrate hands-on techniques with a fluorescence microscope. Constellation microspheres consist of a selected mixture of beads in assorted sizes and colors () that can be used to practice adjusting the focus and switching filters on a fluorescence microscope. These microspheres are stable at room temperature, so they can be conveniently stored. The web link leads to: This product (Catalog number C14837) has been discontinued. Please contactCustomer Service <https://www.thermofisher.com/us/en/home/technical-resources/contact-us.C14837.html?supportType=SL>for possible alternatives. so I suggest you ask ThermoFidher/LifeTech/Invitrogen/Molecular Probes for their current equivalent. My understanding is Constellation was made from the reject lots and leftovers of all of Mol Probes microspheres. Mount in some viscous solution, such as glycerol (something you know or can look up the refractive index; thickness: whatever you want: I would probably put a big drop in a mattek 35mm imaging dish and seal a coverglass on top ... on my "to do list" if/when I get a microscopy job). Acknowledgement: Constallation beads were a lot of fun on a Zeiss TIRF3 microscope demo -- widefield levitation vs TIRF "appear and disappear" -- I cohosted with Justin Price at University of Miami several years ago. My thanks to Kevin Johnson and Katherine Schafer-Hales and others at Zeiss for enabling the demo. George On 1/30/2017 9:17 AM, WAINWRIGHT James wrote: > ***** > To join, leave or search the confocal microscopy listserv, go to: > http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy > Post images onhttp://www.imgur.com and include the link in your posting. > ***** > > Thanks Gary - I'll give them a try. > > Yes - I'm after something with structure, rather than the Chroma plastic slides. > > James > > > > -----Original Message----- > From: Confocal Microscopy List [mailto:[hidden email]] On Behalf Of Gary Laevsky > Sent: 30 January 2017 14:11 > To:[hidden email] > Subject: Re: Hi James, ask Chroma for a box, or a case, of their fluorescent plastic slides ... Re: Thick, low cost fluorescent or auto-fluorescent slides / samples? > > EXTERNAL EMAIL > > ATTACHMENT ADVISORY > > ***** > To join, leave or search the confocal microscopy listserv, go to: > http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy > Post images onhttp://www.imgur.com and include the link in your posting. > ***** > > Brine shrimp? > > On Mon, Jan 30, 2017 at 9:03 AM, John Oreopoulos <[hidden email]> wrote: > >> ***** >> To join, leave or search the confocal microscopy listserv, go to: >> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy >> Post images onhttp://www.imgur.com and include the link in your posting. >> ***** >> >> I think he's looking for something with 3D structure, George, rather >> than just a fluorescent slab. >> >> John Oreopoulos >> >>> On Jan 30, 2017, at 8:43 AM, George McNamara >>> <[hidden email]> >> wrote: >>> ***** >>> To join, leave or search the confocal microscopy listserv, go to: >>> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy >>> Post images onhttp://www.imgur.com and include the link in your >> posting. >>> ***** >>> >>> Hi James, >>> >>> ask Chroma for a box, or a case, of their fluorescent plastic slides >> (you should give them your shipping number to send to you). I believe >> 1 mm thick (maybe more). >>> You can also visit their booth at the next trade show (Biophysics?). >>> >>> >>> sincerely, >>> >>> George >>> >>> >>>> On 1/30/2017 7:09 AM, WAINWRIGHT James wrote: >>>> ***** >>>> To join, leave or search the confocal microscopy listserv, go to: >>>> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy >>>> Post images onhttp://www.imgur.com and include the link in your >> posting. >>>> ***** >>>> >>>> Dear mailing list, >>>> >>>> I'm trying to find some low cost prepared slides that meet the >> following criteria: >>>> 1) Fluorescent, or auto-fluorescent with UV/VIS/NIR. >>>> >>>> 2) Quite thick - at least 100 µm, ideally 200 µm or more. >>>> >>>> 3) Low cost - i.e. ~£10 per slide. Cheaper = better. >>>> >>>> The most success I've had so far is with pollen grain prepared >>>> slides - >> these are wonderfully bright under fluorescence, detailed, low cost, >> but not very thick (typically up to 50 µm). Plant cross-sections such >> as Convallaria are also great, but again, tend to only be 10 µm or so thick. >>>> Many thanks for any ideas or suppliers you can provide, on or off-list. >>>> >>>> James >>>> >>>> >>>> >>>> ___________________________________________________________________ >>>> ________This >> e-mail is confidential and is for the addressee only. Please refer to >> www.oxinst.com/email-statement for regulatory information. >>> -- >>> >>> >>> George McNamara, PhD >>> Houston, TX 77054 >>> [hidden email] >>> https://www.linkedin.com/in/georgemcnamara >>> https://works.bepress.com/gmcnamara/75/ >>> http://www.ncbi.nlm.nih.gov/myncbi/browse/collection/44962650 > > -- > Best, > > Gary Laevsky, Ph.D. > Director, Confocal Imaging Facility > Nikon Center of Excellence > Dept. of Molecular Biology > Washington Rd. > Princeton University > Princeton, New Jersey, 08544-1014 > (O) 609 258 5432 > (C) 508 507 1310 > > > +++Scanned for Viruses by ForcePoint+++ -- George McNamara, PhD Houston, TX 77054 [hidden email] https://www.linkedin.com/in/georgemcnamara https://works.bepress.com/gmcnamara/75/ http://www.ncbi.nlm.nih.gov/myncbi/browse/collection/44962650 |
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Kilgore, Jason A. |
Re: Mosquitoes, ticks, other critters (brine shrimp mentioned previously) ... Re: Thick, low cost fluorescent or auto-fluorescent slides / samples?
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To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy Post images on http://www.imgur.com and include the link in your posting. ***** Just to throw in another option: many flower petals have autofluorescent proteins in them as well as the anthers and stamens, including their pollen, and serve as quick and dirty samples. I was partial to spider plant petals and flowers due to their small size and autofluorescence. Jason Jason A. Kilgore Technical Application Scientist Molecular Probes / EVOS Tech Support Life Sciences Solutions Thermo Fisher Scientific 29851 Willow Creek Rd. Eugene, OR 97402-9132 1-800-955-6288 then option 4, then option 6, then option 2. Or dial direct at +1 541 335 0353 [hidden email] www.lifetechnologies.com This communication is intended solely for the individual/entity to whom it is addressed. It may contain confidential or legally privileged information. Any unauthorized disclosure or copying is prohibited and may be unlawful. If you have received this communication in error, please notify the sender immediately and delete it from your system. -----Original Message----- From: Confocal Microscopy List [mailto:[hidden email]] On Behalf Of [hidden email] Sent: Monday, January 30, 2017 1:59 PM To: [hidden email] Subject: Re: Mosquitoes, ticks, other critters (brine shrimp mentioned previously) ... Re: Thick, low cost fluorescent or auto-fluorescent slides / samples? ***** To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy Post images on http://www.imgur.com and include the link in your posting. ***** And then, of course, there is wood, though you’d probably have to make your own sections on a sliding microtome if you wanted them reasonably thick – the prepared slides are probably less than 20 microns thick. A colleague has just been looking at some wood slices about 100 or so microns thick, from stored pieces of branch fixed in formaldehyde (FAA I think). To soften for sectioning – rinse thoroughly, then a few minutes in the microwave. A wooden drinks coaster might work if you are imaging on the confocal. We used to look at a polished piece of on the confocal as a demo for school students. cheers, Rosemary Dr Rosemary White CSIRO Black Mountain GPO Box 1700 Canberra, ACT 2601 Australia Adjunct Prof, EH Graham Centre, CSU & Research School of Biology, ANU T 61 2 6246 5475 E [hidden email] On 31/1/17, 3:54 am, "Confocal Microscopy List on behalf of George McNamara" <[hidden email] on behalf of [hidden email]> wrote: ***** To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy Post images on http://www.imgur.com and include the link in your posting. ***** * Mosquitoes, ticks, other critters (brine shrimp mentioned previously). * brightest white T-shirt (probably just need a micro piece). Sadly the Constallation microspheres have been discontinued: https://www.thermofisher.com/us/en/home/references/molecular-probes-the-handbook/ultrasensitive-detection-technology/microspheres.html Fluorescent Microspheres for Educational Purposes Constellation microspheres for imaging (C14837 <https://www.thermofisher.com/order/catalog/en/US/adirect/lt?cmd=catProductDetail&entryPoint=adirect&productID=C14837&messageType=catProductDetail>) can be used to demonstrate hands-on techniques with a fluorescence microscope. Constellation microspheres consist of a selected mixture of beads in assorted sizes and colors () that can be used to practice adjusting the focus and switching filters on a fluorescence microscope. These microspheres are stable at room temperature, so they can be conveniently stored. The web link leads to: This product (Catalog number C14837) has been discontinued. Please contactCustomer Service <https://www.thermofisher.com/us/en/home/technical-resources/contact-us.C14837.html?supportType=SL>for possible alternatives. so I suggest you ask ThermoFidher/LifeTech/Invitrogen/Molecular Probes for their current equivalent. My understanding is Constellation was made from the reject lots and leftovers of all of Mol Probes microspheres. Mount in some viscous solution, such as glycerol (something you know or can look up the refractive index; thickness: whatever you want: I would probably put a big drop in a mattek 35mm imaging dish and seal a coverglass on top ... on my "to do list" if/when I get a microscopy job). Acknowledgement: Constallation beads were a lot of fun on a Zeiss TIRF3 microscope demo -- widefield levitation vs TIRF "appear and disappear" -- I cohosted with Justin Price at University of Miami several years ago. My thanks to Kevin Johnson and Katherine Schafer-Hales and others at Zeiss for enabling the demo. George On 1/30/2017 9:17 AM, WAINWRIGHT James wrote: > ***** > To join, leave or search the confocal microscopy listserv, go to: > http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy > Post images onhttp://www.imgur.com and include the link in your posting. > ***** > > Thanks Gary - I'll give them a try. > > Yes - I'm after something with structure, rather than the Chroma plastic slides. > > James > > > > -----Original Message----- > From: Confocal Microscopy List [mailto:[hidden email]] On Behalf Of Gary Laevsky > Sent: 30 January 2017 14:11 > To:[hidden email] > Subject: Re: Hi James, ask Chroma for a box, or a case, of their fluorescent plastic slides ... Re: Thick, low cost fluorescent or auto-fluorescent slides / samples? > > EXTERNAL EMAIL > > ATTACHMENT ADVISORY > > ***** > To join, leave or search the confocal microscopy listserv, go to: > http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy > Post images onhttp://www.imgur.com and include the link in your posting. > ***** > > Brine shrimp? > > On Mon, Jan 30, 2017 at 9:03 AM, John Oreopoulos <[hidden email]> wrote: > >> ***** >> To join, leave or search the confocal microscopy listserv, go to: >> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy >> Post images onhttp://www.imgur.com and include the link in your posting. >> ***** >> >> I think he's looking for something with 3D structure, George, rather >> than just a fluorescent slab. >> >> John Oreopoulos >> >>> On Jan 30, 2017, at 8:43 AM, George McNamara >>> <[hidden email]> >> wrote: >>> ***** >>> To join, leave or search the confocal microscopy listserv, go to: >>> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy >>> Post images onhttp://www.imgur.com and include the link in your >> posting. >>> ***** >>> >>> Hi James, >>> >>> ask Chroma for a box, or a case, of their fluorescent plastic slides >> (you should give them your shipping number to send to you). I believe >> 1 mm thick (maybe more). >>> You can also visit their booth at the next trade show (Biophysics?). >>> >>> >>> sincerely, >>> >>> George >>> >>> >>>> On 1/30/2017 7:09 AM, WAINWRIGHT James wrote: >>>> ***** >>>> To join, leave or search the confocal microscopy listserv, go to: >>>> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy >>>> Post images onhttp://www.imgur.com and include the link in your >> posting. >>>> ***** >>>> >>>> Dear mailing list, >>>> >>>> I'm trying to find some low cost prepared slides that meet the >> following criteria: >>>> 1) Fluorescent, or auto-fluorescent with UV/VIS/NIR. >>>> >>>> 2) Quite thick - at least 100 µm, ideally 200 µm or more. >>>> >>>> 3) Low cost - i.e. ~£10 per slide. Cheaper = better. >>>> >>>> The most success I've had so far is with pollen grain prepared >>>> slides - >> these are wonderfully bright under fluorescence, detailed, low cost, >> but not very thick (typically up to 50 µm). Plant cross-sections such >> as Convallaria are also great, but again, tend to only be 10 µm or so thick. >>>> Many thanks for any ideas or suppliers you can provide, on or off-list. >>>> >>>> James >>>> >>>> >>>> >>>> ___________________________________________________________________ >>>> ________This >> e-mail is confidential and is for the addressee only. Please refer to >> www.oxinst.com/email-statement for regulatory information. >>> -- >>> >>> >>> George McNamara, PhD >>> Houston, TX 77054 >>> [hidden email] >>> https://www.linkedin.com/in/georgemcnamara >>> https://works.bepress.com/gmcnamara/75/ >>> http://www.ncbi.nlm.nih.gov/myncbi/browse/collection/44962650 > > -- > Best, > > Gary Laevsky, Ph.D. > Director, Confocal Imaging Facility > Nikon Center of Excellence > Dept. of Molecular Biology > Washington Rd. > Princeton University > Princeton, New Jersey, 08544-1014 > (O) 609 258 5432 > (C) 508 507 1310 > > > +++Scanned for Viruses by ForcePoint+++ -- George McNamara, PhD Houston, TX 77054 [hidden email] https://www.linkedin.com/in/georgemcnamara https://works.bepress.com/gmcnamara/75/ http://www.ncbi.nlm.nih.gov/myncbi/browse/collection/44962650 |
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phil laissue-2 |
Re: Mosquitoes, ticks, other critters (brine shrimp mentioned previously) ... Re: Thick, low cost fluorescent or auto-fluorescent slides / samples?
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To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy Post images on http://www.imgur.com and include the link in your posting. ***** I regularly use chlorophyll autofluorescence in orange tree lichen, found on ageing and dead tree branches. http://www.nikonsmallworld.com/subjects/image/lichen/3 _________________________________________ Philippe Laissue, PhD Royal Society Industry Fellow School of Biological Sciences, Room 4.17 University of Essex, Colchester CO4 3SQ, UK (0044) 01206 872246 / (0044) 07842 676 456 [hidden email] privatewww.essex.ac.uk/~plaissue On 30 January 2017 at 22:30, Kilgore, Jason A. < [hidden email]> wrote: > ***** > To join, leave or search the confocal microscopy listserv, go to: > http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy > Post images on http://www.imgur.com and include the link in your posting. > ***** > > > Just to throw in another option: many flower petals have autofluorescent > proteins in them as well as the anthers and stamens, including their > pollen, and serve as quick and dirty samples. I was partial to spider > plant petals and flowers due to their small size and autofluorescence. > > Jason > > > > Jason A. Kilgore > Technical Application Scientist > Molecular Probes / EVOS Tech Support > Life Sciences Solutions > > Thermo Fisher Scientific > 29851 Willow Creek Rd. > Eugene, OR 97402-9132 > 1-800-955-6288 then option 4, then option 6, then option 2. > Or dial direct at +1 541 335 0353 > [hidden email] > www.lifetechnologies.com > > This communication is intended solely for the individual/entity to whom it > is addressed. It may contain confidential or legally privileged > information. Any unauthorized disclosure or copying is prohibited and may > be unlawful. If you have received this communication in error, please > notify the sender immediately and delete it from your system. > -----Original Message----- > From: Confocal Microscopy List [mailto:[hidden email]] > On Behalf Of [hidden email] > Sent: Monday, January 30, 2017 1:59 PM > To: [hidden email] > Subject: Re: Mosquitoes, ticks, other critters (brine shrimp mentioned > previously) ... Re: Thick, low cost fluorescent or auto-fluorescent slides > / samples? > > ***** > To join, leave or search the confocal microscopy listserv, go to: > http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy > Post images on http://www.imgur.com and include the link in your posting. > ***** > > And then, of course, there is wood, though you’d probably have to make > your own sections on a sliding microtome if you wanted them reasonably > thick – the prepared slides are probably less than 20 microns thick. A > colleague has just been looking at some wood slices about 100 or so microns > thick, from stored pieces of branch fixed in formaldehyde (FAA I think). To > soften for sectioning – rinse thoroughly, then a few minutes in the > microwave. > > A wooden drinks coaster might work if you are imaging on the confocal. We > used to look at a polished piece of on the confocal as a demo for school > students. > > cheers, > Rosemary > > Dr Rosemary White > CSIRO Black Mountain > GPO Box 1700 > Canberra, ACT 2601 > Australia > Adjunct Prof, EH Graham Centre, CSU > & Research School of Biology, ANU > > T 61 2 6246 5475 > E [hidden email] > > On 31/1/17, 3:54 am, "Confocal Microscopy List on behalf of George > McNamara" <[hidden email] on behalf of > [hidden email]> wrote: > > ***** > To join, leave or search the confocal microscopy listserv, go to: > http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy > Post images on http://www.imgur.com and include the link in your > posting. > ***** > > > * Mosquitoes, ticks, other critters (brine shrimp mentioned > previously). > > * brightest white T-shirt (probably just need a micro piece). > > Sadly the Constallation microspheres have been discontinued: > > https://www.thermofisher.com/us/en/home/references/ > molecular-probes-the-handbook/ultrasensitive-detection- > technology/microspheres.html > > > Fluorescent Microspheres for Educational Purposes > > Constellation microspheres for imaging (C14837 > <https://www.thermofisher.com/order/catalog/en/US/adirect/ > lt?cmd=catProductDetail&entryPoint=adirect&productID=C14837&messageType= > catProductDetail>) > can be used to demonstrate hands-on techniques with a fluorescence > microscope. Constellation microspheres consist of a selected mixture of > beads in assorted sizes and colors () that can be used to practice > adjusting the focus and switching filters on a fluorescence microscope. > These microspheres are stable at room temperature, so they can be > conveniently stored. > > The web link leads to: > > This product (Catalog number C14837) has been discontinued. Please > contactCustomer Service > <https://www.thermofisher.com/us/en/home/technical- > resources/contact-us.C14837.html?supportType=SL>for > possible alternatives. > > > so I suggest you ask ThermoFidher/LifeTech/Invitrogen/Molecular Probes > for their current equivalent. My understanding is Constellation was > made > from the reject lots and leftovers of all of Mol Probes microspheres. > Mount in some viscous solution, such as glycerol (something you know or > can look up the refractive index; thickness: whatever you want: I would > probably put a big drop in a mattek 35mm imaging dish and seal a > coverglass on top ... on my "to do list" if/when I get a microscopy > job). Acknowledgement: Constallation beads were a lot of fun on a Zeiss > TIRF3 microscope demo -- widefield levitation vs TIRF "appear and > disappear" -- I cohosted with Justin Price at University of Miami > several years ago. My thanks to Kevin Johnson and Katherine > Schafer-Hales and others at Zeiss for enabling the demo. > > George > > On 1/30/2017 9:17 AM, WAINWRIGHT James wrote: > > ***** > > To join, leave or search the confocal microscopy listserv, go to: > > http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy > > Post images onhttp://www.imgur.com and include the link in your > posting. > > ***** > > > > Thanks Gary - I'll give them a try. > > > > Yes - I'm after something with structure, rather than the Chroma > plastic slides. > > > > James > > > > > > > > -----Original Message----- > > From: Confocal Microscopy List [mailto:CONFOCALMICROSCOPY@ > LISTS.UMN.EDU] On Behalf Of Gary Laevsky > > Sent: 30 January 2017 14:11 > > To:[hidden email] > > Subject: Re: Hi James, ask Chroma for a box, or a case, of their > fluorescent plastic slides ... Re: Thick, low cost fluorescent or > auto-fluorescent slides / samples? > > > > EXTERNAL EMAIL > > > > ATTACHMENT ADVISORY > > > > ***** > > To join, leave or search the confocal microscopy listserv, go to: > > http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy > > Post images onhttp://www.imgur.com and include the link in your > posting. > > ***** > > > > Brine shrimp? > > > > On Mon, Jan 30, 2017 at 9:03 AM, John Oreopoulos < > [hidden email]> wrote: > > > >> ***** > >> To join, leave or search the confocal microscopy listserv, go to: > >> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy > >> Post images onhttp://www.imgur.com and include the link in your > posting. > >> ***** > >> > >> I think he's looking for something with 3D structure, George, rather > >> than just a fluorescent slab. > >> > >> John Oreopoulos > >> > >>> On Jan 30, 2017, at 8:43 AM, George McNamara > >>> <[hidden email]> > >> wrote: > >>> ***** > >>> To join, leave or search the confocal microscopy listserv, go to: > >>> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy > >>> Post images onhttp://www.imgur.com and include the link in your > >> posting. > >>> ***** > >>> > >>> Hi James, > >>> > >>> ask Chroma for a box, or a case, of their fluorescent plastic > slides > >> (you should give them your shipping number to send to you). I > believe > >> 1 mm thick (maybe more). > >>> You can also visit their booth at the next trade show > (Biophysics?). > >>> > >>> > >>> sincerely, > >>> > >>> George > >>> > >>> > >>>> On 1/30/2017 7:09 AM, WAINWRIGHT James wrote: > >>>> ***** > >>>> To join, leave or search the confocal microscopy listserv, go to: > >>>> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy > >>>> Post images onhttp://www.imgur.com and include the link in your > >> posting. > >>>> ***** > >>>> > >>>> Dear mailing list, > >>>> > >>>> I'm trying to find some low cost prepared slides that meet the > >> following criteria: > >>>> 1) Fluorescent, or auto-fluorescent with UV/VIS/NIR. > >>>> > >>>> 2) Quite thick - at least 100 µm, ideally 200 µm or more. > >>>> > >>>> 3) Low cost - i.e. ~£10 per slide. Cheaper = better. > >>>> > >>>> The most success I've had so far is with pollen grain prepared > >>>> slides - > >> these are wonderfully bright under fluorescence, detailed, low cost, > >> but not very thick (typically up to 50 µm). Plant cross-sections > such > >> as Convallaria are also great, but again, tend to only be 10 µm or > so thick. > >>>> Many thanks for any ideas or suppliers you can provide, on or > off-list. > >>>> > >>>> James > >>>> > >>>> > >>>> > >>>> ____________________________________________________________ > _______ > >>>> ________This > >> e-mail is confidential and is for the addressee only. Please refer > to > >> www.oxinst.com/email-statement for regulatory information. > >>> -- > >>> > >>> > >>> George McNamara, PhD > >>> Houston, TX 77054 > >>> [hidden email] > >>> https://www.linkedin.com/in/georgemcnamara > >>> https://works.bepress.com/gmcnamara/75/ > >>> http://www.ncbi.nlm.nih.gov/myncbi/browse/collection/44962650 > > > > -- > > Best, > > > > Gary Laevsky, Ph.D. > > Director, Confocal Imaging Facility > > Nikon Center of Excellence > > Dept. of Molecular Biology > > Washington Rd. > > Princeton University > > Princeton, New Jersey, 08544-1014 > > (O) 609 258 5432 > > (C) 508 507 1310 > > > > > > +++Scanned for Viruses by ForcePoint+++ > > -- > > > George McNamara, PhD > Houston, TX 77054 > [hidden email] > https://www.linkedin.com/in/georgemcnamara > https://works.bepress.com/gmcnamara/75/ > http://www.ncbi.nlm.nih.gov/myncbi/browse/collection/44962650 > > > |
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In reply to this post by George McNamara
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To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy Post images on http://www.imgur.com and include the link in your posting. ***** Any small zooplankton, daphnia, fairy shrimp, copepod and the suchlike fixed in pfa and stained with acetocarmine or rose bengal (much easier, just add a smidge to the fix and all the animals will turn pink) and optically cleared (we tend to use a TDE protocol) will last for years as a whole mount and autofluoresce beautifully in response to most lasers and across many parts of the spectrum. The internal structure of the beasties shows up wonderfully, especially all the muscle blocks and with detail down to the banding of the muscle fibres under higher mags. See http://imgur.com/a/j0Y8U for a quick and dirty auto assembled tilescan (4x4 from memory) from a Leica SP8 (405, 488 and 561 excitation) of a small beastie stained and mounted a few years ago. |
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Reece, Jeff (NIH/NIDDK) [E] |
Re: Mosquitoes, ticks, other critters (brine shrimp mentioned previously) ... Re: Thick, low cost fluorescent or auto-fluorescent slides / samples?
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In reply to this post by Kilgore, Jason A.
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To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy Post images on http://www.imgur.com and include the link in your posting. ***** On the wood theme: paper products. Not sure if copy paper works. I have used the crappy old business cards with success, learned that from a Zeiss service technician a couple decades ago. Cheers, Jeff -----Original Message----- From: Kilgore, Jason A. [mailto:[hidden email]] Sent: Monday, January 30, 2017 5:30 PM To: [hidden email] Subject: Re: Mosquitoes, ticks, other critters (brine shrimp mentioned previously) ... Re: Thick, low cost fluorescent or auto-fluorescent slides / samples? ***** To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy Post images on http://www.imgur.com and include the link in your posting. ***** Just to throw in another option: many flower petals have autofluorescent proteins in them as well as the anthers and stamens, including their pollen, and serve as quick and dirty samples. I was partial to spider plant petals and flowers due to their small size and autofluorescence. Jason Jason A. Kilgore Technical Application Scientist Molecular Probes / EVOS Tech Support Life Sciences Solutions Thermo Fisher Scientific 29851 Willow Creek Rd. Eugene, OR 97402-9132 1-800-955-6288 then option 4, then option 6, then option 2. Or dial direct at +1 541 335 0353 [hidden email] www.lifetechnologies.com This communication is intended solely for the individual/entity to whom it is addressed. It may contain confidential or legally privileged information. Any unauthorized disclosure or copying is prohibited and may be unlawful. If you have received this communication in error, please notify the sender immediately and delete it from your system. -----Original Message----- From: Confocal Microscopy List [mailto:[hidden email]] On Behalf Of [hidden email] Sent: Monday, January 30, 2017 1:59 PM To: [hidden email] Subject: Re: Mosquitoes, ticks, other critters (brine shrimp mentioned previously) ... Re: Thick, low cost fluorescent or auto-fluorescent slides / samples? ***** To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy Post images on http://www.imgur.com and include the link in your posting. ***** And then, of course, there is wood, though you’d probably have to make your own sections on a sliding microtome if you wanted them reasonably thick – the prepared slides are probably less than 20 microns thick. A colleague has just been looking at some wood slices about 100 or so microns thick, from stored pieces of branch fixed in formaldehyde (FAA I think). To soften for sectioning – rinse thoroughly, then a few minutes in the microwave. A wooden drinks coaster might work if you are imaging on the confocal. We used to look at a polished piece of on the confocal as a demo for school students. cheers, Rosemary Dr Rosemary White CSIRO Black Mountain GPO Box 1700 Canberra, ACT 2601 Australia Adjunct Prof, EH Graham Centre, CSU & Research School of Biology, ANU T 61 2 6246 5475 E [hidden email] On 31/1/17, 3:54 am, "Confocal Microscopy List on behalf of George McNamara" <[hidden email] on behalf of [hidden email]> wrote: ***** To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy Post images on http://www.imgur.com and include the link in your posting. ***** * Mosquitoes, ticks, other critters (brine shrimp mentioned previously). * brightest white T-shirt (probably just need a micro piece). Sadly the Constallation microspheres have been discontinued: https://www.thermofisher.com/us/en/home/references/molecular-probes-the-handbook/ultrasensitive-detection-technology/microspheres.html Fluorescent Microspheres for Educational Purposes Constellation microspheres for imaging (C14837 <https://www.thermofisher.com/order/catalog/en/US/adirect/lt?cmd=catProductDetail&entryPoint=adirect&productID=C14837&messageType=catProductDetail>) can be used to demonstrate hands-on techniques with a fluorescence microscope. Constellation microspheres consist of a selected mixture of beads in assorted sizes and colors () that can be used to practice adjusting the focus and switching filters on a fluorescence microscope. These microspheres are stable at room temperature, so they can be conveniently stored. The web link leads to: This product (Catalog number C14837) has been discontinued. Please contactCustomer Service <https://www.thermofisher.com/us/en/home/technical-resources/contact-us.C14837.html?supportType=SL>for possible alternatives. so I suggest you ask ThermoFidher/LifeTech/Invitrogen/Molecular Probes for their current equivalent. My understanding is Constellation was made from the reject lots and leftovers of all of Mol Probes microspheres. Mount in some viscous solution, such as glycerol (something you know or can look up the refractive index; thickness: whatever you want: I would probably put a big drop in a mattek 35mm imaging dish and seal a coverglass on top ... on my "to do list" if/when I get a microscopy job). Acknowledgement: Constallation beads were a lot of fun on a Zeiss TIRF3 microscope demo -- widefield levitation vs TIRF "appear and disappear" -- I cohosted with Justin Price at University of Miami several years ago. My thanks to Kevin Johnson and Katherine Schafer-Hales and others at Zeiss for enabling the demo. George On 1/30/2017 9:17 AM, WAINWRIGHT James wrote: > ***** > To join, leave or search the confocal microscopy listserv, go to: > http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy > Post images onhttp://www.imgur.com and include the link in your posting. > ***** > > Thanks Gary - I'll give them a try. > > Yes - I'm after something with structure, rather than the Chroma plastic slides. > > James > > > > -----Original Message----- > From: Confocal Microscopy List [mailto:[hidden email]] On Behalf Of Gary Laevsky > Sent: 30 January 2017 14:11 > To:[hidden email] > Subject: Re: Hi James, ask Chroma for a box, or a case, of their fluorescent plastic slides ... Re: Thick, low cost fluorescent or auto-fluorescent slides / samples? > > EXTERNAL EMAIL > > ATTACHMENT ADVISORY > > ***** > To join, leave or search the confocal microscopy listserv, go to: > http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy > Post images onhttp://www.imgur.com and include the link in your posting. > ***** > > Brine shrimp? > > On Mon, Jan 30, 2017 at 9:03 AM, John Oreopoulos <[hidden email]> wrote: > >> ***** >> To join, leave or search the confocal microscopy listserv, go to: >> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy >> Post images onhttp://www.imgur.com and include the link in your posting. >> ***** >> >> I think he's looking for something with 3D structure, George, rather >> than just a fluorescent slab. >> >> John Oreopoulos >> >>> On Jan 30, 2017, at 8:43 AM, George McNamara >>> <[hidden email]> >> wrote: >>> ***** >>> To join, leave or search the confocal microscopy listserv, go to: >>> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy >>> Post images onhttp://www.imgur.com and include the link in your >> posting. >>> ***** >>> >>> Hi James, >>> >>> ask Chroma for a box, or a case, of their fluorescent plastic slides >> (you should give them your shipping number to send to you). I believe >> 1 mm thick (maybe more). >>> You can also visit their booth at the next trade show (Biophysics?). >>> >>> >>> sincerely, >>> >>> George >>> >>> >>>> On 1/30/2017 7:09 AM, WAINWRIGHT James wrote: >>>> ***** >>>> To join, leave or search the confocal microscopy listserv, go to: >>>> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy >>>> Post images onhttp://www.imgur.com and include the link in your >> posting. >>>> ***** >>>> >>>> Dear mailing list, >>>> >>>> I'm trying to find some low cost prepared slides that meet the >> following criteria: >>>> 1) Fluorescent, or auto-fluorescent with UV/VIS/NIR. >>>> >>>> 2) Quite thick - at least 100 µm, ideally 200 µm or more. >>>> >>>> 3) Low cost - i.e. ~£10 per slide. Cheaper = better. >>>> >>>> The most success I've had so far is with pollen grain prepared >>>> slides - >> these are wonderfully bright under fluorescence, detailed, low cost, >> but not very thick (typically up to 50 µm). Plant cross-sections such >> as Convallaria are also great, but again, tend to only be 10 µm or so thick. >>>> Many thanks for any ideas or suppliers you can provide, on or off-list. >>>> >>>> James >>>> >>>> >>>> >>>> ___________________________________________________________________ >>>> ________This >> e-mail is confidential and is for the addressee only. Please refer to >> www.oxinst.com/email-statement for regulatory information. >>> -- >>> >>> >>> George McNamara, PhD >>> Houston, TX 77054 >>> [hidden email] >>> https://www.linkedin.com/in/georgemcnamara >>> https://works.bepress.com/gmcnamara/75/ >>> http://www.ncbi.nlm.nih.gov/myncbi/browse/collection/44962650 > > -- > Best, > > Gary Laevsky, Ph.D. > Director, Confocal Imaging Facility > Nikon Center of Excellence > Dept. of Molecular Biology > Washington Rd. > Princeton University > Princeton, New Jersey, 08544-1014 > (O) 609 258 5432 > (C) 508 507 1310 > > > +++Scanned for Viruses by ForcePoint+++ -- George McNamara, PhD Houston, TX 77054 [hidden email] https://www.linkedin.com/in/georgemcnamara https://works.bepress.com/gmcnamara/75/ http://www.ncbi.nlm.nih.gov/myncbi/browse/collection/44962650 |
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