Mounting non fixed tissue ?

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Jean-Pierre CLAMME-2 Jean-Pierre CLAMME-2
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Mounting non fixed tissue ?

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Hi,

I was wondering if anyone knows about the possibility of mounting thin
tissue sections without fixing the tissue. What would be a protocol for
such samples ?
All the protocol I've found involve fixing.

Thank you.

JP
MORONE Diego RIC MORONE Diego RIC
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Re: Mounting non fixed tissue ?

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Dear Jean-Pierre,

I use a drop of 100% glycerol to mount fresh thick tissues, it should work with thin tissues. You could also try vetbond surgical glue

Best
Diego


------------------------------------------
DAI IL TUO 5x1000 ALLA RICERCA HUMANITAS.

Scegli Fondazione Humanitas per la Ricerca.

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------------------------------------------
-----Original Message-----
From: Jean-Pierre CLAMME [mailto:[hidden email]]
Sent: venerdì 8 novembre 2013 20:11
Subject: Mounting non fixed tissue ?

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Hi,

I was wondering if anyone knows about the possibility of mounting thin tissue sections without fixing the tissue. What would be a protocol for such samples ?
All the protocol I've found involve fixing.

Thank you.

JP
Craig Brideau Craig Brideau
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Re: Mounting non fixed tissue ?

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Vetbond works somewhat; I've seen it used to tack down dorsal root, etc. We
usually use nets to hold tissue down in a tiny perfusion chamber though, as
this keeps it happy (or at least less sad...) during imaging.


On Mon, Nov 11, 2013 at 6:23 AM, MORONE Diego RIC <
[hidden email]> wrote:

> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> *****
>
> Dear Jean-Pierre,
>
> I use a drop of 100% glycerol to mount fresh thick tissues, it should work
> with thin tissues. You could also try vetbond surgical glue
>
> Best
> Diego
>
>
> ------------------------------------------
> DAI IL TUO 5x1000 ALLA RICERCA HUMANITAS.
>
> Scegli Fondazione Humanitas per la Ricerca.
>
> Codice fiscale 97408620157 (Ricerca Scientifica e Università)
>
> http://www.iomerito.it
> ------------------------------------------
> -----Original Message-----
> From: Jean-Pierre CLAMME [mailto:[hidden email]]
> Sent: venerdì 8 novembre 2013 20:11
> Subject: Mounting non fixed tissue ?
>
> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> *****
>
> Hi,
>
> I was wondering if anyone knows about the possibility of mounting thin
> tissue sections without fixing the tissue. What would be a protocol for
> such samples ?
> All the protocol I've found involve fixing.
>
> Thank you.
>
> JP
>
Doube, Michael Doube, Michael
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Re: Mounting non fixed tissue ?

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> Vetbond works somewhat

Vetbond is n-butyl cyanoacrylate (similar to superglue).

http://www.3m.com/product/information/Vetbond-Tissue-Adhesive.html

Any experience with the other cyanoacrylates out there?

Michael


<http://www.rvc.ac.uk>

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