Hege,
Here is my 2 cents. I have an SP5 with a Mai Tai
HP using NDD in complete dark. For intra-vital of blood vessels (mouse
foot) we typically get 300um, in live spinal core, 400-500 and brain, fix &
stained 300-400 depending. I do scan the excitation to determine the optimal
excitation wavelength for each flurophore. For some unknown, at least to
me, I have experienced differences in optimal excitation wavelength from system
to system. I complete agree with Guy on the other points.
Hope that this helps
Richard
Cole
Research Scientist V
Director: Advanced Light
Microscopy
& Image Analysis
Core
Wadsworth Center
P.O. Box 509 Albany
N.Y. 12201-0509
Research Assistant Professor
Dept.
of Biomedical Sciences
School
of Public Health
State University
of New York Albany, New York
( 518-474-7048
Ê 518-474-4430
* [hidden email]
Website www.wadsworth.org/cores/alm/index.htm
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