Multi well plastic plates/ Leica AFC

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Pascal Weber Pascal Weber
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Multi well plastic plates/ Leica AFC

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Hello everyone, I just bought the AFC at Leica objectives and long distances
for acquisition of live cell culture in plastic boxes. The problem is that it does
not work (primarily) with glass. Unsuitable for cell culture and their
development. By cons it works with the 10X objective. With 20 and 40 the  
AFC  happens to be locked but the cells are blurry. My opinion is that the
bottom of the box is too thick. Does someone know a brand of plastic box
bottom 170μ thick?
George McNamara George McNamara
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Re: Multi well plastic plates/ Leica AFC

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http://www.irori.com/pages/technology/keyAttributes.html

http://www.whatman.com/References/GlassBotClearViewMicroDataSheetFINAL3.26.10LR.pdf

BD Falcon has an equivalent to the Whatman Clearview


On 5/7/2012 2:50 AM, Pascal Weber wrote:

> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> *****
>
> Hello everyone, I just bought the AFC at Leica objectives and long distances
> for acquisition of live cell culture in plastic boxes. The problem is that it does
> not work (primarily) with glass. Unsuitable for cell culture and their
> development. By cons it works with the 10X objective. With 20 and 40 the
> AFC  happens to be locked but the cells are blurry. My opinion is that the
> bottom of the box is too thick. Does someone know a brand of plastic box
> bottom 170μ thick?
>
>