Mike,
Invitrogen and Pierce are both good companies but the former, as you
know, bought up Molecular Probes (MP) whose company focus was to make
end products for microscopists. Pierce was and I assume is still the
best supplier of reagents, columns, i.e., the raw materials for do it
yourselfers like me. Often I would use a reactive fluorophore
derivative from MP but dialysis cassettes, and small purification
columns from Pierce and also some specialty reagents such as the -SH
reducer TCEP, an excellent compound for revealing thiols for
dye-maleimide conjugation that does not need removal from the treated
protein before applying the maleimide as other disulfide reducers do.
I have in previous postings sung the praises of the Invitrogen Zenon
labeling kits, which use an Fab-Alexa dye (various colors) to bind
with a primary antibody before labeling cells. With the Zenon-labeled
primaries I successfully labeled cells on slides, and in cell
suspensions whether for confocal or flow cytometry.
Email me if you want more details. Both are great companies.
(Disclaimer: no financial interest on my part).
Mario
>Could someone recommend an IgG labeling kit? There are kits made by
>Invitrogen, Pierce, and others. Which in your experience is the best?
>Thanks!
>
>Mike
>
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________________________________________________________________________________
Mario M. Moronne, Ph.D.
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