NEW DETECTORS?

[Brian O'Rourke-3]

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Hi all,
After several years break from the list I'm back on with an old
question that is perpetually new. It is:

What are people's recent multiphoton/confocal imaging experiences with new
detectors like the hybrid photodetector (e.g. H8236 PMT/APD) from
Hamamatsu or the new silicon PMTs (e.g. from SensL)? They seem to have
nice fast responses and the gains are getting better (10^6 for the
latter). Are PMTs still the popular choice?

Related question: What bandwidth for PMT analog I-V conversion is
required for fast scanning? Is 200KHz okay or does one need > 1MHz?
Or, are you using photon counting instead of analog, these days?

My two incentives for asking are: 1) I'm trying to keep my external
detectors alive a bit longer on our ancient BioRad 1024MP and 2) I'm
working on setting up another system.

Thanks,
Brian O'Rourke

[Guy Cox]

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I've used the Leica APD detector (at the factory, I don't
have one).  Sensitivity is amazing but you can get frustrated
since if the signal gets too strong it just cuts off and all
goes black (rather than just saturated as happens on a
conventional PMT).  So you have to be careful setting up a
Z-stack, and if there's a bright spot in your image
when you want to look at dim structures you also have a
problem.  

                                                Guy



Optical Imaging Techniques in Cell Biology
by Guy Cox    CRC Press / Taylor & Francis
    http://www.guycox.com/optical.htm
______________________________________________
Associate Professor Guy Cox, MA, DPhil(Oxon)
Electron Microscope Unit, Madsen Building F09,
University of Sydney, NSW 2006
______________________________________________
Phone [phone]     Fax [phone]
Mobile [phone]
______________________________________________
     http://www.guycox.net
-----Original Message-----
From: Confocal Microscopy List [mailto:[hidden email]] On Behalf Of Brian O'Rourke
Sent: Friday, 29 August 2008 4:22 AM
To: [hidden email]
Subject: NEW DETECTORS?

Search the CONFOCAL archive at
http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal

Hi all,
After several years break from the list I'm back on with an old question that is perpetually new. It is:

What are people's recent multiphoton/confocal imaging experiences with new detectors like the hybrid photodetector (e.g. H8236 PMT/APD) from Hamamatsu or the new silicon PMTs (e.g. from SensL)? They seem to have nice fast responses and the gains are getting better (10^6 for the latter). Are PMTs still the popular choice?

Related question: What bandwidth for PMT analog I-V conversion is required for fast scanning? Is 200KHz okay or does one need > 1MHz?
Or, are you using photon counting instead of analog, these days?

My two incentives for asking are: 1) I'm trying to keep my external detectors alive a bit longer on our ancient BioRad 1024MP and 2) I'm working on setting up another system.

Thanks,
Brian O'Rourke

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