NIH FAES course on Immunofluorescence and Confocal

[Reece, Jeff (NIH/NIDDK) [E]]

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Dear List Members,

There are still spaces available for the course described below, with more detail at:
http://events.r20.constantcontact.com/register/event?oeidk=a07eb3041605ca8f809&llr=k4uamblab

BioTech 35: Immunofluorescence and Confocal Microscopy
January 19-22, 2016
Location: National Institutes of Health, Bethesda, MD USA

Program Description: Through morning classes and afternoon hands-on lab sessions, Biotech 35 will focus on two important methods that are used extensively in biomedical research. Confocal microscopy has emerged as a powerful and popular extension of fluorescence microscopy, allowing 3-dimensional localization and dynamics of cellular components. The course is designed as a "boot camp" for those cell biologists just entering the world of confocal microscopy, wishing to utilize the technology to its fullest potential, with fixed and live specimens, on commercially available equipment. Several related, advanced topics are introduced to give participants an overview of future possibilities.

Classroom topics include: Fluorescence; Immunofluorescence; Confocal Microscopy; Specimen Preparation; 2-Photon Excitation; Colocalization of Proteins; Spectral Unmixing; FRET; FRAP and FLIP; PhotoActivation; Image Processing and Analysis using Fiji; SPIM; TIRF; STED; SMLM (GSD, PALM, STORM, PAINT); SIM; Deconvolution.

Labs: Rotating in groups of 4 or less, participants will gain hands-on experience with: Fixed Sample Preparation for Fluorescence/Confocal Microscopy; Acquisition/Optimization of Basic Confocal Scans using Single-point Laser-Scanning Confocal Microscopes, Multiphoton excitation, and Spinning Disk Confocal; Colocalization using multi-color fluorescence imaging; FRET; Spectral Unmixing; Live Cell Imaging: Translocation and localization of proteins using fluorescent fusion proteins and time lapse imaging; Protein Dynamics Using FRAP, FLIP, and PA-GFP; Image Processing and Analysis using Fiji.

Who Should Attend?  Biomedical Scientists just entering the world of confocal microscopy.

Instructors are experts in the field from both academia/government and industry.

Feel free to contact me directly if you have questions pertaining to the content of the course.  Registration is handled at the web site above.

Warm Regards,
Jeff

Jeff Reece
Ph: +1.301.451.4330
E:  [hidden email]<https://mail.nih.gov/owa/14.3.174.1/scripts/premium/redir.aspx?C=vorg2hwQ3EG79HF4VARC2_-txi1AZNEITAaQhKx2WUBLeDOG3BM2dSsWeRsCBbyhbstXsPzU2G8.&URL=mailto%3ajeff.reece%40nih.gov>

Director, Advanced Light Microscopy & Image Analysis Core (ALMIAC)
NIH/NIDDK
Bethesda, MD  20892

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