New Addition to Optical Microscopy Course, Marine Biology Lab, Woods Hole, MA

[Hard, Robert]

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New Addition to Optical Microscopy and Imaging Course

We are announcing an exciting addition to this year’s Optical Microscopy and Imaging Course at the Marine Biology Laboratory, Woods Hole, MA (Sept. 29-Oct. 9, 2013).

We will be constructing a structured illumination microscope for super-resolution imaging. We also will have available a super-resolution FRET system for data acquisition. Along with all the other instrumentation in the course, these super-res systems will be available by appointment for visualizing student research samples.

For those who may wish to take advantage of this opportunity and have not yet applied, we have extended the deadline for applications until June 22, 2013.

Details are described below.


Course Directors,

Dr. Robert Hard              Dr. Hari Shroff
University at Buffalo       NIH

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THIS IS AN ANNOUNCEMENT FOR THE OMIBS COURSE, MARINE BIOLOGY LABORATORY, WOODS HOLE, MA:

Title: Optical Microscopy and Imaging in the Biomedical Sciences

History: Established in 1978 and offered annually since then.

When: September 29 - October 9, 2013

Application Deadline: Applications accepted through June 22, 2013

Course Directors:

Dr. Robert Hard
Dept. Pathology and Anatomical Sciences
School of Medicine and Biomedical Sciences
University at Buffalo
Phone: [716] 829-3521
Email: [hidden email]

Dr. Hari Shroff
NIH/NIBIB
Section on High Resolution Optical Imaging
National Institutes of Health
Phone: [301] 435-1995
Email: [hidden email]


COURSE DESCRIPTION:

Where: In the newly renovated and modernized facilities in the Loeb Bldg at the Marine Biology Laboratory, Woods Hole, MA

What: Imaging fundamentals to state of the art, multi-dimensional wide field, fluorescence, confocal, multiphoton, multispectral, deconvolution, and super-resolution microscopy systems.

How: Lectures, hands on exercises, problem sets, demonstrations and discussions, specifically designed to drive home the principles and applications of modern microscopy. Students will learn to obtain and interpret microscope images of high quality, to perform quantitative optical measurements, and to produce digital records for documentation and analysis. In the problem sets, small groups of students will acquire, analyze, interpret and present imaging data, thereby demonstrating their progress toward mastering microscopy and imaging skills.

Equipment: Microscopes, Cameras, and Software from 19 World Renowned Companies.

For Whom:
Designed primarily for research scientists, postdoctoral trainees and advanced graduate students in animal, plant, and medical sciences. Facility Directors and non-biologists seeking a comprehensive introduction to microscopy and digital imaging in the biomedical sciences will benefit greatly from this course. There are no specific prerequisites, but an understanding of the basic principles of optics and imaging is desirable. The course is limited to 26 students.

Specimens: Live and fixed specimens will be provided for use in laboratory exercises and demonstrations. However, students are encouraged to bring their own biological (cell cultures, prepared slides, etc. ) and material specimens to use in exercises throughout the course, where appropriate. Cell culture facilities are available for student use. Students also are encouraged to make appointments with our experienced Commercial Faculty outside of scheduled classes to acquire data from their specimens using the state of the art equipment available. Students are highly encouraged to discuss their individual research problems with both the academic and commercial faculty.

Topics To Be Covered Include:

Principles of microscope design and image formation, image resolution, and image contrast.

Bright field, dark field, phase contrast, polarized light, differential interference contrast, interference reflection, and fluorescence microscopy

Cameras, digital imaging, signal to noise ratio, recording, enhancement, processing and analysis

Advanced fluorescence techniques (eg. FRAP, FLIP, speckle imaging, FRET, FLIM, fluorescence polarization, photoactivation, photoswitching, fluorescence correlation spectroscopy, multi-spectral imaging, filter cubes, fluorescent dyes/proteins)

Confocal scanning microscopy (LSM and spinning disc), multiphoton excitation fluorescence microscopy and structured illumination systems

Image deconvolution, digital image restoration

3D image acquisition, display, and analysis

Live cell imaging

Super-resolution techniques (STED, PALM, STORM, SIM)


Tuition: $3220 (Includes room and board, text, handouts, supplies)

Admission application and information:
Carol Hamel, Admissions Coordinator
Marine Biological Laboratory
7 MBL Street
Woods Hole, MA 02543-1015
[phone]
Internet: [hidden email]
WWW: http://www.mbl.edu (Application forms available via Adobe Acrobat)


Recent faculty have included:

Frank Bright, University at Buffalo
Richard Day, Indiana University School of Medicine
Joseph A DePasquale, Morphogenyx
Paul Goodwin, Applied Precision, Inc.
Brian Herman, U. Texas Health Science Center
Shinya Inoue, Marine Biology Laboratory
Frederick Lanni, Carnegie Mellon University
Jennifer Lippincott-Schwartz, Cell Biology and Metabolism, NIH
James McNally, National Cancer Institute, NIH
Butch Moomaw, Hamamatsu Corp.
John M. Murray, Indiana University
Rudolf Oldenberg, Marine Biology Laboratory
David Piston, Vanderbilt University
Michael Shribak, Marine Biology Laboratory
Wade Sigurdson, University at Buffalo
Clare M. Waterman, Cell Biology and Physiology Center, NIH


See Also:
http://www.mbl.edu/education/courses/special_topics/om.html