*****
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http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy*****
*For more information visit
http://meetings.cshl.edu/courses/c-qicm11.shtml**QUANTITATIVE IMAGING:
FROM CELLS TO MOLECULES*
October 24 - November 6, 2011
Application Deadline: July 15, 2011
Instructors:
Bo Huang <
http://huanglab.ucsf.edu/Research.html>*, University of California
San Francisco
*Max Krummel <
http://bms.ucsf.edu/directory/faculty/matthew-krummel-phd>,
*University
of California San Francisco*
Alison North <
http://www.rockefeller.edu/bioimaging/>, *The Rockefeller
University*
Samara Reck-Peterson <
https://reck-peterson.med.harvard.edu/>, *Harvard
University*
Torsten Wittmann<
http://bms.ucsf.edu/directory/faculty/torsten-wittmann-phd>,
*University of California San Francisco*
Jennifer Waters <
http://cellbio.med.harvard.edu/faculty/waters/index.html>,
*Harvard University
* This course will focus on advanced quantitative fluorescence microscopy
techniques used for imaging a range of biological specimens, from cells to
single molecules. The course is designed for cell and molecular biologists
with little or no microscopy experience, who wish to begin utilizing
microscopy in their own research. Students will gain a theoretical
understanding of, and hands-on experience with, state-of-the-art equipment
used in quantitative fluorescence microscopy, including: laser scanning and
spinning disk confocal microscopy, deconvolution methods, total internal
fluorescence microscopy (TIRF), super-resolution methods (structured
illumination, STORM, and PALM), and digital image processing and analysis.
Students will learn how to design and implement a wide range of imaging
experiments using these techniques. Students will use the techniques to
address specific quantitative questions and then discuss the results as a
group, learning to trouble-shoot the common problems that occur in the
course of a quantitative imaging experiment. Among the lectures presented
are: microscopy basics, CCD cameras, confocal microscopy, multi-photon
microscopy, deconvolution, TIRF, single molecule imaging, imaging
ratio-metric “biosensors” (including FRET), and super-resolution techniques.
Students will also learn specimen preparation for microscopy, including
fixation and immunofluorescence in tissue culture cells, choosing
fluorescent proteins, working with live samples requiring environmental
control, and more.
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