New paper at DiasproLab of potential interest plus 3 anticipations

[Alberto Diaspro]

*****
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Post images on http://www.imgur.com and include the link in your posting.
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The new paper - mainly developed by Cristiano Viappiani lab in Parma - is on
Subdiffraction localization of a nanostructured photosensitizer in bacterial cells
http://www.nature.com/articles/srep15564
———

On december 2015 1-4 2nd Practical Workshop on Advanced Microscopy @ www.nic.iit.it
Confirmed speakers: Sara Abrahamsson, Martin Oheim, Silvia Galiani, Chiara Cordiglieri, Colin JR Sheppard, Laura Cancedda
First day: open access talks. Following days: practicals, only 30 seats available - free.

Interested researchers: email to [hidden email]  AND [hidden email]  - subject (mandatory “2nd NIC Workshop 2015”)
(please add short cv and motivation)

——

“Pop Microscopy” exhibition at Parma University 2015-2016

we still need 5 microscopy images to close the collection: 1 high resolution image, author, title, technical details, motivation of the research behind
this call closes when we reach 5 useful images

———

Have a nice weekend
Alby

http://www.iit.it/it/about-naph/alberto-diaspro.html

https://twitter.com/AD1959

https://scholar.google.com/citations?user=FtRb-LIAAAAJ&hl=it

[George McNamara]

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To join, leave or search the confocal microscopy listserv, go to:
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Post images on http://www.imgur.com and include the link in your posting.
*****

Hi Alby,

I provide web links to download my entries. If Parma has a building with
a big white wall, I suggest projecting Tiki_Goddess on it throughout the
event.

Entry 1 (several resolutions available: Tiki_Goddess

Available online at different resolutions;

http://works.bepress.com/gmcnamara/11/
or
http://works.bepress.com/gmcnamara/70/
if you don't need so high res, or want to compare several side by side,
http://works.bepress.com/gmcnamara/1/
also available at
http://home.earthlink.net/~tiki_goddess/TikiGoddess.jpg

backstory at
http://home.earthlink.net/~tiki_goddess/
(with out of date contact information except for my earthlink email
address) and at "11".

author: George McNamara

title: Tiki_Goddess

technical details: Young mouse tissue section, Masson's trichrome stain,
slide sold by Carolina Biological Supply.

http://works.bepress.com/gmcnamara/11/ acquired on Olympus Nanozoomer
(Hamamatsu Nanozoomer distributed by Olympus. 20x objective lens, if I
recall correctly, no recollection of NA). My apology to
Olympus/Hamamatsu for forgetting who arranged the demonstration for us
at UMiami.
http://works.bepress.com/gmcnamara/70/ acquired with ThorLabs TDI
Whole-Slide-Scanning Microscope (TDI = time delay integration). More
information at
http://www.thorlabs.com/NewGroupPage9.cfm?ObjectGroup_ID=6570 and
https://www.youtube.com/watch?v=bUkhhoOxWE0 ... and my thanks to Ash
Prabala and his team at ThorLabs Imaging, Austin, TX, for the
opportunity to scan Tiki_Goddess (no commercial interests on my part).

motivation of the research: scan entire microscope slide specimen (40 mm
long in this case) in "one click".

I have Tiki_Goddess printed as a poster on "fabric" in the hallway of
our lab here at M.D. Anderson Cancer Center. Was also the "cover girl"
of the May 2015 Biotechniques,
http://www.biotechniques.com/BiotechniquesJournal/2015/May/

entry 2: Halloween
http://works.bepress.com/gmcnamara/25/

I encourage everyone on the Confocal Listserv to play the Halloween Game at
https://www.linkedin.com/pulse/happy-halloween-george-mcnamara

I especially encourage Alby, Jeremy Adler, Claire Brown, and Michelle
Peckham -- and if they read this, Jennifer Lippincott-Schwartz, Claire
Waterman, Rebecca Richards-Kortun, Sally Ward, Xiaowei Zhuang, Melody
Swartz, Katrin Willig, and Evelin Schrock (re my post,
http://lists.umn.edu/cgi-bin/wa?A2=ind1510&L=CONFOCALMICROSCOPY&D=0&P=11890 
).

FYI - for your marketing department: good opportunity to put out a press
release and invite trick or treaters to the Pop Microscopy event.

Technical details (very short version): The photo is a collage of human
HeLa (Henrietta Lacks cervical cancer cell line) cells I acquired for
two users (40x/1.3NA J.G., 63x/1.4NA, R.R., both Leica oil immersion
objective lenses, initials were all the information I still have on the
users who made the slides; camera was an Applied Spectral Imaging SD-300
spectral imager in monochrome acquisition mode).

Motivation for research: originally for immunofluorescence and/or DNA
FISH of whatever J.G. and R.R. were studying.
Now: No calorie fun for Halloween.


entry 3: Cosmic Ray Particles visualized (83.33 hours observation in one
TIFF image file).

http://works.bepress.com/gmcnamara/19/
see web page  for more technical details. Briefly: Hamamatsu ORCA-ER
camera, 30,000 exposures, 10 seconds per exposure. Cameras was on a
Zeiss Axiovert 200M microscope that I did my best to block any light
into the camera. Acquisition was controlled by MetaMorph Imaging System.

Additional data related to this:
http://works.bepress.com/gmcnamara/20/
http://works.bepress.com/gmcnamara/24/
http://onlinedigeditions.com/publication/?i=167312  see this for the
Microscopy Today article I wrote.

Motivation: Celebrate the 101th anniversary of the discovery of cosmic
ray particles (that is, Centenary + 1, discovery was August 7, 1912) -
the discoverer won the Nobel Prize in Physics (initially thought to be
"rays", now known to be particles, including iron ions travelling at
high velocity). Secondary motivation: even dark exposures can have
signal beyond the thermal and electronic backgrounds.

//

Video entry (if you use it, please find a high resolution projector or
monitor, and send me a photo of yourself and other organizers next to it):

Title: Rgers PMN Movie Panorama 2012 The Chase
Availability:  http://works.bepress.com/gmcnamara/18/       avi video file.
                    http://works.bepress.com/gmcnamara/5/         
earlier version as MetaMorph .STK stack file.

technical details: see my four MetaMatters newsletters for details (also
text in the video explains history):
http://works.bepress.com/gmcnamara/73/

Motivation:
1. convert classic cell motility film/video from "jumpy" original to
Panorama.
2. digitize for computers the neutrophil shapes and locations (section 2
of the video has the outlines).
3. show off temporal area maps and temporal area map histograms for
quantifying cel motility.
Note: "The Chase" was named by, and won, Mary David (presenter)
honorable mention at an ASCB meeting video contest a couple of years ago.



You did not mention copyright, so I will make clear: The images and
videos are data, data are facts, facts cannot be copyrighted.  I discuss
this point in our PubSpectra paper (open access)
http://onlinelibrary.wiley.com/doi/10.1002/cyto.a.20304/abstract
"PubSpectra is our free download site which uses Microsoft Excel files
as standardized human/machine readable format with over 2,000 biomedical
spectra. The principle that data is not subject to copyright provides a
framework in which all scientific data should be made freely accessible. "
Note: the PubSpectra data is now hosted at
http://works.bepress.com/gmcnamara/9/
because I revised the original site to host two electronic posters on
multiplexing fluorescent proteins and especially FP biosensors, and
temporal area maps, respectively,
http://home.earthlink.net/~pubspectra/McNamara_20121023Tue_Tattletales_GFP_Public_Domain.jpg
and
http://home.earthlink.net/~pubspectra/McNamara_20121023Tue_TAM_and_Tattletales_TIK_Public_Domain.jpg 

Yesterday, 10/23/2015, was the third anniversary of my posting those
online into the public domain - TALE-lights, TALE-color, SUNtag, and
other similar schemes have been published by others since. See
http://works.bepress.com/gmcnamara/65/
for some of my late 2014 suggestions with respect to binary Tattletales
and T-Bow (multiplex, localized, transcriptional rainbow reporters --
complete coincidence that it is pronounced similarly to the last name of
Tim Tebow0.

Enjoy,

George
p.s. congratulations on better gender balance for your NIC workshop.


On 10/24/2015 8:30 AM, Alberto Diaspro wrote:

> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> Post images on http://www.imgur.com and include the link in your posting.
> *****
>
> The new paper - mainly developed by Cristiano Viappiani lab in Parma - is on
> Subdiffraction localization of a nanostructured photosensitizer in bacterial cells
> http://www.nature.com/articles/srep15564
> ———
>
> On december 2015 1-4 2nd Practical Workshop on Advanced Microscopy @ www.nic.iit.it
> Confirmed speakers: Sara Abrahamsson, Martin Oheim, Silvia Galiani, Chiara Cordiglieri, Colin JR Sheppard, Laura Cancedda
> First day: open access talks. Following days: practicals, only 30 seats available - free.
>
> Interested researchers: email to [hidden email]  AND [hidden email]  - subject (mandatory “2nd NIC Workshop 2015”)
> (please add short cv and motivation)
>
> ——
>
> “Pop Microscopy” exhibition at Parma University 2015-2016
>
> we still need 5 microscopy images to close the collection: 1 high resolution image, author, title, technical details, motivation of the research behind
> this call closes when we reach 5 useful images
>
> ———
>
> Have a nice weekend
> Alby
>
> http://www.iit.it/it/about-naph/alberto-diaspro.html
>
> https://twitter.com/AD1959
>
> https://scholar.google.com/citations?user=FtRb-LIAAAAJ&hl=it
>
>    

--



George McNamara, Ph.D.
Single Cells Analyst
L.J.N. Cooper Lab
University of Texas M.D. Anderson Cancer Center
Houston, TX 77054
Tattletales http://works.bepress.com/gmcnamara/42
http://works.bepress.com/gmcnamara/65/
https://www.linkedin.com/in/georgemcnamara
https://www.linkedin.com/pulse/happy-halloween-george-mcnamara