Nikon SoRa and Zeiss Lattice SIM

[Romin, Yevgeniy/Sloan Kettering Institute]

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Hello everybody


I was wondering if anybody here has had any experience with the Nikon SoRa and Zeiss Elyra 7 with lattice SIM.  I know that the two systems attain super-resolution images in different ways, but just wanted to learn about people's experiences with these systems from the point of fast super-res imaging of live and fixed samples and general attributes, such as sensitivity.  Any insights you can share on the subject will be much appreciated.


Thanks very much in advance,


Yevgeniy Romin

=====================================================================

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[samuel connell]

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A quick commercial note. The Yokogawa SoRa is available from a handful of
commercial suppliers, including Nikon, 3i, Olympus, Solamere, and Visitron.

We have found the SoRa to be particularly easy to use and appreciate the
flexibility of its integration into a standard spinning disk system. (Quite
straight-forward to add photomanipulation options as well.) Like lots of
microscopy systems, one must take great care with the initial system
alignment, but once the system is locked down it is rock solid. The core
sensitivity is driven by the camera choice and honestly, different camera
options with different S:N capabilities is a nice benefit of the SoRa
design. It's also relatively cost-effective to have a SoRa with a pair of
cameras either for simultaneous imaging or for a set of distinct camera
choices. The SoRa is somewhat unique in its capacity to provide confocal
optical sectioning with SIM. This is atypical and opens up a wider array of
sample choices that are amenable to the SIM technology.

Feel free to reach out with more specific questions if you'd like.

--
Sam

Samuel Connell
Director of Sales

Intelligent Imaging Innovations (3i)
3509 Ringsby Court
Denver, CO  80216  USA
1-720-437-6926
[hidden email]
www.intelligent-imaging.com

On Wed, Jul 17, 2019 at 5:55 AM <[hidden email]> wrote:

> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> Post images on http://www.imgur.com and include the link in your posting.
> *****
>
> Hello everybody
>
>
> I was wondering if anybody here has had any experience with the Nikon SoRa
> and Zeiss Elyra 7 with lattice SIM.  I know that the two systems attain
> super-resolution images in different ways, but just wanted to learn about
> people's experiences with these systems from the point of fast super-res
> imaging of live and fixed samples and general attributes, such as
> sensitivity.  Any insights you can share on the subject will be much
> appreciated.
>
>
> Thanks very much in advance,
>
>
> Yevgeniy Romin
>
> =====================================================================
>
>      Please note that this e-mail and any files transmitted from
>      Memorial Sloan Kettering Cancer Center may be privileged,
> confidential,
>      and protected from disclosure under applicable law. If the reader of
>      this message is not the intended recipient, or an employee or agent
>      responsible for delivering this message to the intended recipient,
>      you are hereby notified that any reading, dissemination, distribution,
>      copying, or other use of this communication or any of its attachments
>      is strictly prohibited.  If you have received this communication in
>      error, please notify the sender immediately by replying to this
> message
>      and deleting this message, any attachments, and all copies and backups
>      from your computer.
>

[Alison J. North]

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*****

Hi Sam,

Your points are very useful, except that I wouldn't entirely agree that
the SoRa is "somewhat unique" in doing SIM with confocal sectioning. 
Hari Shroff and Andy York's iSIM, commercialized by VisiTech quite some
time before the SoRa was released, does exactly that too, no?  Needless
to say, I am defending my own purchase decision here :-).  And I also
think it's useful to point out that there are even more exciting
possibilities now by combining Paul Maddox's Mizar TILT with this kind
of system, which is what we have just done.   Light sheet and super-res
on the same system, easy to combine within a single timelapse
experiment?  Exciting times!!!

All the best,

Alison


On 7/18/2019 12:09 PM, samuel connell wrote:

> *****
> To join, leave or search the confocal microscopy listserv, go to:
> https://urldefense.proofpoint.com/v2/url?u=http-3A__lists.umn.edu_cgi-2Dbin_wa-3FA0-3Dconfocalmicroscopy&d=DwIBaQ&c=JeTkUgVztGMmhKYjxsy2rfoWYibK1YmxXez1G3oNStg&r=RBx0-WJrAO5vwSOLNmFbqYvikvIZS5ns3-USwvMOuLo&m=MG4gB93r8Ahe6bFz16x4LGVehA_9uWkzEX5Eh3QltPM&s=-I9dYnY9gnXFdJHzix4nDwhcKmaMfCPC8xzSt4dQ7HI&e=
> Post images on https://urldefense.proofpoint.com/v2/url?u=http-3A__www.imgur.com&d=DwIBaQ&c=JeTkUgVztGMmhKYjxsy2rfoWYibK1YmxXez1G3oNStg&r=RBx0-WJrAO5vwSOLNmFbqYvikvIZS5ns3-USwvMOuLo&m=MG4gB93r8Ahe6bFz16x4LGVehA_9uWkzEX5Eh3QltPM&s=PJ1EvDrAqe26jiSTx4_pVDJfGZLOdaPQ3b5b_XLzeDo&e=  and include the link in your posting.
> *****
>
> A quick commercial note. The Yokogawa SoRa is available from a handful of
> commercial suppliers, including Nikon, 3i, Olympus, Solamere, and Visitron.
>
> We have found the SoRa to be particularly easy to use and appreciate the
> flexibility of its integration into a standard spinning disk system. (Quite
> straight-forward to add photomanipulation options as well.) Like lots of
> microscopy systems, one must take great care with the initial system
> alignment, but once the system is locked down it is rock solid. The core
> sensitivity is driven by the camera choice and honestly, different camera
> options with different S:N capabilities is a nice benefit of the SoRa
> design. It's also relatively cost-effective to have a SoRa with a pair of
> cameras either for simultaneous imaging or for a set of distinct camera
> choices. The SoRa is somewhat unique in its capacity to provide confocal
> optical sectioning with SIM. This is atypical and opens up a wider array of
> sample choices that are amenable to the SIM technology.
>
> Feel free to reach out with more specific questions if you'd like.
>
> --
> Sam
>
> Samuel Connell
> Director of Sales
>
> Intelligent Imaging Innovations (3i)
> 3509 Ringsby Court
> Denver, CO  80216  USA
> 1-720-437-6926
> [hidden email]
> www.intelligent-imaging.com
>
> On Wed, Jul 17, 2019 at 5:55 AM <[hidden email]> wrote:
>
>> *****
>> To join, leave or search the confocal microscopy listserv, go to:
>> https://urldefense.proofpoint.com/v2/url?u=http-3A__lists.umn.edu_cgi-2Dbin_wa-3FA0-3Dconfocalmicroscopy&d=DwIBaQ&c=JeTkUgVztGMmhKYjxsy2rfoWYibK1YmxXez1G3oNStg&r=RBx0-WJrAO5vwSOLNmFbqYvikvIZS5ns3-USwvMOuLo&m=MG4gB93r8Ahe6bFz16x4LGVehA_9uWkzEX5Eh3QltPM&s=-I9dYnY9gnXFdJHzix4nDwhcKmaMfCPC8xzSt4dQ7HI&e=
>> Post images on https://urldefense.proofpoint.com/v2/url?u=http-3A__www.imgur.com&d=DwIBaQ&c=JeTkUgVztGMmhKYjxsy2rfoWYibK1YmxXez1G3oNStg&r=RBx0-WJrAO5vwSOLNmFbqYvikvIZS5ns3-USwvMOuLo&m=MG4gB93r8Ahe6bFz16x4LGVehA_9uWkzEX5Eh3QltPM&s=PJ1EvDrAqe26jiSTx4_pVDJfGZLOdaPQ3b5b_XLzeDo&e=  and include the link in your posting.
>> *****
>>
>> Hello everybody
>>
>>
>> I was wondering if anybody here has had any experience with the Nikon SoRa
>> and Zeiss Elyra 7 with lattice SIM.  I know that the two systems attain
>> super-resolution images in different ways, but just wanted to learn about
>> people's experiences with these systems from the point of fast super-res
>> imaging of live and fixed samples and general attributes, such as
>> sensitivity.  Any insights you can share on the subject will be much
>> appreciated.
>>
>>
>> Thanks very much in advance,
>>
>>
>> Yevgeniy Romin
>>
>> =====================================================================
>>
>>       Please note that this e-mail and any files transmitted from
>>       Memorial Sloan Kettering Cancer Center may be privileged,
>> confidential,
>>       and protected from disclosure under applicable law. If the reader of
>>       this message is not the intended recipient, or an employee or agent
>>       responsible for delivering this message to the intended recipient,
>>       you are hereby notified that any reading, dissemination, distribution,
>>       copying, or other use of this communication or any of its attachments
>>       is strictly prohibited.  If you have received this communication in
>>       error, please notify the sender immediately by replying to this
>> message
>>       and deleting this message, any attachments, and all copies and backups
>>       from your computer.
>>

--
Alison J. North, Ph.D.,
Research Associate Professor and
Senior Director of the Bio-Imaging Resource Center,
The Rockefeller University,
1230 York Avenue,
New York,
NY 10065.
Tel: office    ++ 212 327 7488
Tel: lab        ++ 212 327 7486
Fax:            ++ 212 327 7489

[samuel connell]

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*****

Hi Alison,

Your points are well received. I was more focused on Yevgeniey's question
about those two particular technologies so my term "somewhat unique" was
even more ambiguous than it sounded in my head.
(In fact, Andrew likes to call the SoRa the York-ogawa.)
I love the inclusion of the TILT on that instrument for a very flexible
live-cell rig. If you were really crazy, you could add a TIRF illuminator
and a FRAP system for multi-modal live-cell imaging.

Cheers,
-
Sam




On Thu, Jul 18, 2019 at 11:12 AM Alison J. North <
[hidden email]> wrote:

> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> Post images on http://www.imgur.com and include the link in your posting.
> *****
>
> Hi Sam,
>
> Your points are very useful, except that I wouldn't entirely agree that
> the SoRa is "somewhat unique" in doing SIM with confocal sectioning.
> Hari Shroff and Andy York's iSIM, commercialized by VisiTech quite some
> time before the SoRa was released, does exactly that too, no?  Needless
> to say, I am defending my own purchase decision here :-).  And I also
> think it's useful to point out that there are even more exciting
> possibilities now by combining Paul Maddox's Mizar TILT with this kind
> of system, which is what we have just done.   Light sheet and super-res
> on the same system, easy to combine within a single timelapse
> experiment?  Exciting times!!!
>
> All the best,
>
> Alison
>
>
> On 7/18/2019 12:09 PM, samuel connell wrote:
> > *****
> > To join, leave or search the confocal microscopy listserv, go to:
> >
> https://urldefense.proofpoint.com/v2/url?u=http-3A__lists.umn.edu_cgi-2Dbin_wa-3FA0-3Dconfocalmicroscopy&d=DwIBaQ&c=JeTkUgVztGMmhKYjxsy2rfoWYibK1YmxXez1G3oNStg&r=RBx0-WJrAO5vwSOLNmFbqYvikvIZS5ns3-USwvMOuLo&m=MG4gB93r8Ahe6bFz16x4LGVehA_9uWkzEX5Eh3QltPM&s=-I9dYnY9gnXFdJHzix4nDwhcKmaMfCPC8xzSt4dQ7HI&e=
> > Post images on
> https://urldefense.proofpoint.com/v2/url?u=http-3A__www.imgur.com&d=DwIBaQ&c=JeTkUgVztGMmhKYjxsy2rfoWYibK1YmxXez1G3oNStg&r=RBx0-WJrAO5vwSOLNmFbqYvikvIZS5ns3-USwvMOuLo&m=MG4gB93r8Ahe6bFz16x4LGVehA_9uWkzEX5Eh3QltPM&s=PJ1EvDrAqe26jiSTx4_pVDJfGZLOdaPQ3b5b_XLzeDo&e=
> and include the link in your posting.
> > *****
> >
> > A quick commercial note. The Yokogawa SoRa is available from a handful of
> > commercial suppliers, including Nikon, 3i, Olympus, Solamere, and
> Visitron.
> >
> > We have found the SoRa to be particularly easy to use and appreciate the
> > flexibility of its integration into a standard spinning disk system.
> (Quite
> > straight-forward to add photomanipulation options as well.) Like lots of
> > microscopy systems, one must take great care with the initial system
> > alignment, but once the system is locked down it is rock solid. The core
> > sensitivity is driven by the camera choice and honestly, different camera
> > options with different S:N capabilities is a nice benefit of the SoRa
> > design. It's also relatively cost-effective to have a SoRa with a pair of
> > cameras either for simultaneous imaging or for a set of distinct camera
> > choices. The SoRa is somewhat unique in its capacity to provide confocal
> > optical sectioning with SIM. This is atypical and opens up a wider array
> of
> > sample choices that are amenable to the SIM technology.
> >
> > Feel free to reach out with more specific questions if you'd like.
> >
> > --
> > Sam
> >
> > Samuel Connell
> > Director of Sales
> >
> > Intelligent Imaging Innovations (3i)
> > 3509 Ringsby Court
> > Denver, CO  80216  USA
> > 1-720-437-6926
> > [hidden email]
> > www.intelligent-imaging.com
> >
> > On Wed, Jul 17, 2019 at 5:55 AM <[hidden email]> wrote:
> >
> >> *****
> >> To join, leave or search the confocal microscopy listserv, go to:
> >>
> https://urldefense.proofpoint.com/v2/url?u=http-3A__lists.umn.edu_cgi-2Dbin_wa-3FA0-3Dconfocalmicroscopy&d=DwIBaQ&c=JeTkUgVztGMmhKYjxsy2rfoWYibK1YmxXez1G3oNStg&r=RBx0-WJrAO5vwSOLNmFbqYvikvIZS5ns3-USwvMOuLo&m=MG4gB93r8Ahe6bFz16x4LGVehA_9uWkzEX5Eh3QltPM&s=-I9dYnY9gnXFdJHzix4nDwhcKmaMfCPC8xzSt4dQ7HI&e=
> >> Post images on
> https://urldefense.proofpoint.com/v2/url?u=http-3A__www.imgur.com&d=DwIBaQ&c=JeTkUgVztGMmhKYjxsy2rfoWYibK1YmxXez1G3oNStg&r=RBx0-WJrAO5vwSOLNmFbqYvikvIZS5ns3-USwvMOuLo&m=MG4gB93r8Ahe6bFz16x4LGVehA_9uWkzEX5Eh3QltPM&s=PJ1EvDrAqe26jiSTx4_pVDJfGZLOdaPQ3b5b_XLzeDo&e=
> and include the link in your posting.
> >> *****
> >>
> >> Hello everybody
> >>
> >>
> >> I was wondering if anybody here has had any experience with the Nikon
> SoRa
> >> and Zeiss Elyra 7 with lattice SIM.  I know that the two systems attain
> >> super-resolution images in different ways, but just wanted to learn
> about
> >> people's experiences with these systems from the point of fast super-res
> >> imaging of live and fixed samples and general attributes, such as
> >> sensitivity.  Any insights you can share on the subject will be much
> >> appreciated.
> >>
> >>
> >> Thanks very much in advance,
> >>
> >>
> >> Yevgeniy Romin
> >>
> >> =====================================================================
> >>
> >>       Please note that this e-mail and any files transmitted from
> >>       Memorial Sloan Kettering Cancer Center may be privileged,
> >> confidential,
> >>       and protected from disclosure under applicable law. If the reader
> of
> >>       this message is not the intended recipient, or an employee or
> agent
> >>       responsible for delivering this message to the intended recipient,
> >>       you are hereby notified that any reading, dissemination,
> distribution,
> >>       copying, or other use of this communication or any of its
> attachments
> >>       is strictly prohibited.  If you have received this communication
> in
> >>       error, please notify the sender immediately by replying to this
> >> message
> >>       and deleting this message, any attachments, and all copies and
> backups
> >>       from your computer.
> >>
> --
> Alison J. North, Ph.D.,
> Research Associate Professor and
> Senior Director of the Bio-Imaging Resource Center,
> The Rockefeller University,
> 1230 York Avenue,
> New York,
> NY 10065.
> Tel: office    ++ 212 327 7488
> Tel: lab        ++ 212 327 7486
> Fax:            ++ 212 327 7489
>
>

[Vitaly Boyko-2]

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*****

 Dear Sam, Alison, and others,
I am sorry but, could you and others, please provide examples of lateral and axial resolution of each system mentioned above with side-by-side comparison of let say Tom20/OxPhos stained cells imaged under similar if not identical conditions, at least with the same N.A. lens, with SoRa, iSIM, lattice SIM, OMX and any other half-super-res system followed by quantitative intensity based image analysis that include analysis of weak and bright objects before and after decomvolution, with theoretical and experimentally obtained PSF, and/or assessing error rate during watershed segmentation of membrane targeted GFP or similar membrane marker(s) with larger samples of at least 100 um along z-axis.
I would greatly appreciate professional guidance by showing side-by-side good examples that have been segmented with identical algorithms.
Best regards,
VitalyMCCF/MSKCC   
    On Thursday, July 18, 2019, 02:39:19 PM EDT, samuel connell <[hidden email]> wrote:  
 
 *****
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Post images on http://www.imgur.com and include the link in your posting.
*****

Hi Alison,

Your points are well received. I was more focused on Yevgeniey's question
about those two particular technologies so my term "somewhat unique" was
even more ambiguous than it sounded in my head.
(In fact, Andrew likes to call the SoRa the York-ogawa.)
I love the inclusion of the TILT on that instrument for a very flexible
live-cell rig. If you were really crazy, you could add a TIRF illuminator
and a FRAP system for multi-modal live-cell imaging.

Cheers,
-
Sam




On Thu, Jul 18, 2019 at 11:12 AM Alison J. North <
[hidden email]> wrote:

> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> Post images on http://www.imgur.com and include the link in your posting.
> *****
>
> Hi Sam,
>
> Your points are very useful, except that I wouldn't entirely agree that
> the SoRa is "somewhat unique" in doing SIM with confocal sectioning.
> Hari Shroff and Andy York's iSIM, commercialized by VisiTech quite some
> time before the SoRa was released, does exactly that too, no?  Needless
> to say, I am defending my own purchase decision here :-).  And I also
> think it's useful to point out that there are even more exciting
> possibilities now by combining Paul Maddox's Mizar TILT with this kind
> of system, which is what we have just done.  Light sheet and super-res
> on the same system, easy to combine within a single timelapse
> experiment?  Exciting times!!!
>
> All the best,
>
> Alison
>
>
> On 7/18/2019 12:09 PM, samuel connell wrote:
> > *****
> > To join, leave or search the confocal microscopy listserv, go to:
> >
> https://urldefense.proofpoint.com/v2/url?u=http-3A__lists.umn.edu_cgi-2Dbin_wa-3FA0-3Dconfocalmicroscopy&d=DwIBaQ&c=JeTkUgVztGMmhKYjxsy2rfoWYibK1YmxXez1G3oNStg&r=RBx0-WJrAO5vwSOLNmFbqYvikvIZS5ns3-USwvMOuLo&m=MG4gB93r8Ahe6bFz16x4LGVehA_9uWkzEX5Eh3QltPM&s=-I9dYnY9gnXFdJHzix4nDwhcKmaMfCPC8xzSt4dQ7HI&e=
> > Post images on
> https://urldefense.proofpoint.com/v2/url?u=http-3A__www.imgur.com&d=DwIBaQ&c=JeTkUgVztGMmhKYjxsy2rfoWYibK1YmxXez1G3oNStg&r=RBx0-WJrAO5vwSOLNmFbqYvikvIZS5ns3-USwvMOuLo&m=MG4gB93r8Ahe6bFz16x4LGVehA_9uWkzEX5Eh3QltPM&s=PJ1EvDrAqe26jiSTx4_pVDJfGZLOdaPQ3b5b_XLzeDo&e=
> and include the link in your posting.
> > *****
> >
> > A quick commercial note. The Yokogawa SoRa is available from a handful of
> > commercial suppliers, including Nikon, 3i, Olympus, Solamere, and
> Visitron.
> >
> > We have found the SoRa to be particularly easy to use and appreciate the
> > flexibility of its integration into a standard spinning disk system.
> (Quite
> > straight-forward to add photomanipulation options as well.) Like lots of
> > microscopy systems, one must take great care with the initial system
> > alignment, but once the system is locked down it is rock solid. The core
> > sensitivity is driven by the camera choice and honestly, different camera
> > options with different S:N capabilities is a nice benefit of the SoRa
> > design. It's also relatively cost-effective to have a SoRa with a pair of
> > cameras either for simultaneous imaging or for a set of distinct camera
> > choices. The SoRa is somewhat unique in its capacity to provide confocal
> > optical sectioning with SIM. This is atypical and opens up a wider array
> of
> > sample choices that are amenable to the SIM technology.
> >
> > Feel free to reach out with more specific questions if you'd like.
> >
> > --
> > Sam
> >
> > Samuel Connell
> > Director of Sales
> >
> > Intelligent Imaging Innovations (3i)
> > 3509 Ringsby Court
> > Denver, CO  80216  USA
> > 1-720-437-6926
> > [hidden email]
> > www.intelligent-imaging.com
> >
> > On Wed, Jul 17, 2019 at 5:55 AM <[hidden email]> wrote:
> >
> >> *****
> >> To join, leave or search the confocal microscopy listserv, go to:
> >>
> https://urldefense.proofpoint.com/v2/url?u=http-3A__lists.umn.edu_cgi-2Dbin_wa-3FA0-3Dconfocalmicroscopy&d=DwIBaQ&c=JeTkUgVztGMmhKYjxsy2rfoWYibK1YmxXez1G3oNStg&r=RBx0-WJrAO5vwSOLNmFbqYvikvIZS5ns3-USwvMOuLo&m=MG4gB93r8Ahe6bFz16x4LGVehA_9uWkzEX5Eh3QltPM&s=-I9dYnY9gnXFdJHzix4nDwhcKmaMfCPC8xzSt4dQ7HI&e=
> >> Post images on
> https://urldefense.proofpoint.com/v2/url?u=http-3A__www.imgur.com&d=DwIBaQ&c=JeTkUgVztGMmhKYjxsy2rfoWYibK1YmxXez1G3oNStg&r=RBx0-WJrAO5vwSOLNmFbqYvikvIZS5ns3-USwvMOuLo&m=MG4gB93r8Ahe6bFz16x4LGVehA_9uWkzEX5Eh3QltPM&s=PJ1EvDrAqe26jiSTx4_pVDJfGZLOdaPQ3b5b_XLzeDo&e=
> and include the link in your posting.
> >> *****
> >>
> >> Hello everybody
> >>
> >>
> >> I was wondering if anybody here has had any experience with the Nikon
> SoRa
> >> and Zeiss Elyra 7 with lattice SIM.  I know that the two systems attain
> >> super-resolution images in different ways, but just wanted to learn
> about
> >> people's experiences with these systems from the point of fast super-res
> >> imaging of live and fixed samples and general attributes, such as
> >> sensitivity.  Any insights you can share on the subject will be much
> >> appreciated.
> >>
> >>
> >> Thanks very much in advance,
> >>
> >>
> >> Yevgeniy Romin
> >>
> >> =====================================================================
> >>
> >>      Please note that this e-mail and any files transmitted from
> >>      Memorial Sloan Kettering Cancer Center may be privileged,
> >> confidential,
> >>      and protected from disclosure under applicable law. If the reader
> of
> >>      this message is not the intended recipient, or an employee or
> agent
> >>      responsible for delivering this message to the intended recipient,
> >>      you are hereby notified that any reading, dissemination,
> distribution,
> >>      copying, or other use of this communication or any of its
> attachments
> >>      is strictly prohibited.  If you have received this communication
> in
> >>      error, please notify the sender immediately by replying to this
> >> message
> >>      and deleting this message, any attachments, and all copies and
> backups
> >>      from your computer.
> >>
> --
> Alison J. North, Ph.D.,
> Research Associate Professor and
> Senior Director of the Bio-Imaging Resource Center,
> The Rockefeller University,
> 1230 York Avenue,
> New York,
> NY 10065.
> Tel: office    ++ 212 327 7488
> Tel: lab        ++ 212 327 7486
> Fax:            ++ 212 327 7489
>
>  

[0000001ed7f52e4a-dmarc-request]

*****
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Post images on http://www.imgur.com and include the link in your posting.
*****

Dear Vitally,
Good points, but you are asking for a lot... I would also add ease of use, reliability and cost to the list :-) or wait 10 years and count the number of publications using these, compared with conventional widefield, confocal and other super-resolution techniques. Maybe with a focus on scientific breakthroughs?
Best wishes
Andreas


-----Original Message-----
From: Vitaly Boyko <[hidden email]>
To: CONFOCALMICROSCOPY <[hidden email]>
Sent: Fri, Jul 19, 2019 04:10 AM
Subject: Re: Nikon SoRa and Zeiss Lattice SIM


*****
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*****

 Dear Sam, Alison, and others,
I am sorry but, could you and others, please provide examples of lateral and axial resolution of each system mentioned above with side-by-side comparison of let say Tom20/OxPhos stained cells imaged under similar if not identical conditions, at least with the same N.A. lens, with SoRa, iSIM, lattice SIM, OMX and any other half-super-res system followed by quantitative intensity based image analysis that include analysis of weak and bright objects before and after decomvolution, with theoretical and experimentally obtained PSF, and/or assessing error rate during watershed segmentation of membrane targeted GFP or similar membrane marker(s) with larger samples of at least 100 um along z-axis.
I would greatly appreciate professional guidance by showing side-by-side good examples that have been segmented with identical algorithms.
Best regards,
VitalyMCCF/MSKCC   
    On Thursday, July 18, 2019, 02:39:19 PM EDT, samuel connell <[hidden email]> wrote: 
 
 *****
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*****

Hi Alison,

Your points are well received. I was more focused on Yevgeniey's question
about those two particular technologies so my term "somewhat unique" was
even more ambiguous than it sounded in my head.
(In fact, Andrew likes to call the SoRa the York-ogawa.)
I love the inclusion of the TILT on that instrument for a very flexible
live-cell rig. If you were really crazy, you could add a TIRF illuminator
and a FRAP system for multi-modal live-cell imaging.

Cheers,
-
Sam




On Thu, Jul 18, 2019 at 11:12 AM Alison J. North <
[hidden email]> wrote:

> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> Post images on http://www.imgur.com and include the link in your posting.
> *****
>
> Hi Sam,
>
> Your points are very useful, except that I wouldn't entirely agree that
> the SoRa is "somewhat unique" in doing SIM with confocal sectioning.
> Hari Shroff and Andy York's iSIM, commercialized by VisiTech quite some
> time before the SoRa was released, does exactly that too, no?  Needless
> to say, I am defending my own purchase decision here :-).  And I also
> think it's useful to point out that there are even more exciting
> possibilities now by combining Paul Maddox's Mizar TILT with this kind
> of system, which is what we have just done.  Light sheet and super-res
> on the same system, easy to combine within a single timelapse
> experiment?  Exciting times!!!
>
> All the best,
>
> Alison
>
>
> On 7/18/2019 12:09 PM, samuel connell wrote:
> > *****
> > To join, leave or search the confocal microscopy listserv, go to:
> >
> https://urldefense.proofpoint.com/v2/url?u=http-3A__lists.umn.edu_cgi-2Dbin_wa-3FA0-3Dconfocalmicroscopy&d=DwIBaQ&c=JeTkUgVztGMmhKYjxsy2rfoWYibK1YmxXez1G3oNStg&r=RBx0-WJrAO5vwSOLNmFbqYvikvIZS5ns3-USwvMOuLo&m=MG4gB93r8Ahe6bFz16x4LGVehA_9uWkzEX5Eh3QltPM&s=-I9dYnY9gnXFdJHzix4nDwhcKmaMfCPC8xzSt4dQ7HI&e=
> > Post images on
> https://urldefense.proofpoint.com/v2/url?u=http-3A__www.imgur.com&d=DwIBaQ&c=JeTkUgVztGMmhKYjxsy2rfoWYibK1YmxXez1G3oNStg&r=RBx0-WJrAO5vwSOLNmFbqYvikvIZS5ns3-USwvMOuLo&m=MG4gB93r8Ahe6bFz16x4LGVehA_9uWkzEX5Eh3QltPM&s=PJ1EvDrAqe26jiSTx4_pVDJfGZLOdaPQ3b5b_XLzeDo&e=
> and include the link in your posting.
> > *****
> >
> > A quick commercial note. The Yokogawa SoRa is available from a handful of
> > commercial suppliers, including Nikon, 3i, Olympus, Solamere, and
> Visitron.
> >
> > We have found the SoRa to be particularly easy to use and appreciate the
> > flexibility of its integration into a standard spinning disk system.
> (Quite
> > straight-forward to add photomanipulation options as well.) Like lots of
> > microscopy systems, one must take great care with the initial system
> > alignment, but once the system is locked down it is rock solid. The core
> > sensitivity is driven by the camera choice and honestly, different camera
> > options with different S:N capabilities is a nice benefit of the SoRa
> > design. It's also relatively cost-effective to have a SoRa with a pair of
> > cameras either for simultaneous imaging or for a set of distinct camera
> > choices. The SoRa is somewhat unique in its capacity to provide confocal
> > optical sectioning with SIM. This is atypical and opens up a wider array
> of
> > sample choices that are amenable to the SIM technology.
> >
> > Feel free to reach out with more specific questions if you'd like.
> >
> > --
> > Sam
> >
> > Samuel Connell
> > Director of Sales
> >
> > Intelligent Imaging Innovations (3i)
> > 3509 Ringsby Court
> > Denver, CO  80216  USA
> > 1-720-437-6926
> > [hidden email]
> > www.intelligent-imaging.com
> >
> > On Wed, Jul 17, 2019 at 5:55 AM <[hidden email]> wrote:
> >
> >> *****
> >> To join, leave or search the confocal microscopy listserv, go to:
> >>
> https://urldefense.proofpoint.com/v2/url?u=http-3A__lists.umn.edu_cgi-2Dbin_wa-3FA0-3Dconfocalmicroscopy&d=DwIBaQ&c=JeTkUgVztGMmhKYjxsy2rfoWYibK1YmxXez1G3oNStg&r=RBx0-WJrAO5vwSOLNmFbqYvikvIZS5ns3-USwvMOuLo&m=MG4gB93r8Ahe6bFz16x4LGVehA_9uWkzEX5Eh3QltPM&s=-I9dYnY9gnXFdJHzix4nDwhcKmaMfCPC8xzSt4dQ7HI&e=
> >> Post images on
> https://urldefense.proofpoint.com/v2/url?u=http-3A__www.imgur.com&d=DwIBaQ&c=JeTkUgVztGMmhKYjxsy2rfoWYibK1YmxXez1G3oNStg&r=RBx0-WJrAO5vwSOLNmFbqYvikvIZS5ns3-USwvMOuLo&m=MG4gB93r8Ahe6bFz16x4LGVehA_9uWkzEX5Eh3QltPM&s=PJ1EvDrAqe26jiSTx4_pVDJfGZLOdaPQ3b5b_XLzeDo&e=
> and include the link in your posting.
> >> *****
> >>
> >> Hello everybody
> >>
> >>
> >> I was wondering if anybody here has had any experience with the Nikon
> SoRa
> >> and Zeiss Elyra 7 with lattice SIM.  I know that the two systems attain
> >> super-resolution images in different ways, but just wanted to learn
> about
> >> people's experiences with these systems from the point of fast super-res
> >> imaging of live and fixed samples and general attributes, such as
> >> sensitivity.  Any insights you can share on the subject will be much
> >> appreciated.
> >>
> >>
> >> Thanks very much in advance,
> >>
> >>
> >> Yevgeniy Romin
> >>
> >> =====================================================================
> >>
> >>      Please note that this e-mail and any files transmitted from
> >>      Memorial Sloan Kettering Cancer Center may be privileged,
> >> confidential,
> >>      and protected from disclosure under applicable law. If the reader
> of
> >>      this message is not the intended recipient, or an employee or
> agent
> >>      responsible for delivering this message to the intended recipient,
> >>      you are hereby notified that any reading, dissemination,
> distribution,
> >>      copying, or other use of this communication or any of its
> attachments
> >>      is strictly prohibited.  If you have received this communication
> in
> >>      error, please notify the sender immediately by replying to this
> >> message
> >>      and deleting this message, any attachments, and all copies and
> backups
> >>      from your computer.
> >>
> --
> Alison J. North, Ph.D.,
> Research Associate Professor and
> Senior Director of the Bio-Imaging Resource Center,
> The Rockefeller University,
> 1230 York Avenue,
> New York,
> NY 10065.
> Tel: office    ++ 212 327 7488
> Tel: lab        ++ 212 327 7486
> Fax:            ++ 212 327 7489
>
> 

[Craig Brideau]

*****
To join, leave or search the confocal microscopy listserv, go to:
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Post images on http://www.imgur.com and include the link in your posting.
*****

You are also assuming that sample preparation will be identical at both
sites etc etc. For a truly fair comparison you should demo the equipment
with your own samples under your own conditions. A test using the sample
you plan to image, or at least a positive control, is the only way to be
confident a given instrument will perform as necessary.

Craig

On Fri, Jul 19, 2019 at 12:52 AM Andreas Bruckbauer <
[hidden email]> wrote:

> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> Post images on http://www.imgur.com and include the link in your posting.
> *****
>
> Dear Vitally,
> Good points, but you are asking for a lot... I would also add ease of use,
> reliability and cost to the list :-) or wait 10 years and count the number
> of publications using these, compared with conventional widefield, confocal
> and other super-resolution techniques. Maybe with a focus on scientific
> breakthroughs?
> Best wishes
> Andreas
>
>
> -----Original Message-----
> From: Vitaly Boyko <[hidden email]>
> To: CONFOCALMICROSCOPY <[hidden email]>
> Sent: Fri, Jul 19, 2019 04:10 AM
> Subject: Re: Nikon SoRa and Zeiss Lattice SIM
>
>
> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> Post images on http://www.imgur.com and include the link in your posting.
> *****
>
>  Dear Sam, Alison, and others,
> I am sorry but, could you and others, please provide examples of lateral
> and axial resolution of each system mentioned above with side-by-side
> comparison of let say Tom20/OxPhos stained cells imaged under similar if
> not identical conditions, at least with the same N.A. lens, with SoRa,
> iSIM, lattice SIM, OMX and any other half-super-res system followed by
> quantitative intensity based image analysis that include analysis of weak
> and bright objects before and after decomvolution, with theoretical and
> experimentally obtained PSF, and/or assessing error rate during watershed
> segmentation of membrane targeted GFP or similar membrane marker(s) with
> larger samples of at least 100 um along z-axis.
> I would greatly appreciate professional guidance by showing side-by-side
> good examples that have been segmented with identical algorithms.
> Best regards,
> VitalyMCCF/MSKCC
>     On Thursday, July 18, 2019, 02:39:19 PM EDT, samuel connell <
> [hidden email]> wrote:
>
>  *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> Post images on http://www.imgur.com and include the link in your posting.
> *****
>
> Hi Alison,
>
> Your points are well received. I was more focused on Yevgeniey's question
> about those two particular technologies so my term "somewhat unique" was
> even more ambiguous than it sounded in my head.
> (In fact, Andrew likes to call the SoRa the York-ogawa.)
> I love the inclusion of the TILT on that instrument for a very flexible
> live-cell rig. If you were really crazy, you could add a TIRF illuminator
> and a FRAP system for multi-modal live-cell imaging.
>
> Cheers,
> -
> Sam
>
>
>
>
> On Thu, Jul 18, 2019 at 11:12 AM Alison J. North <
> [hidden email]> wrote:
>
> > *****
> > To join, leave or search the confocal microscopy listserv, go to:
> > http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> > Post images on http://www.imgur.com and include the link in your
> posting.
> > *****
> >
> > Hi Sam,
> >
> > Your points are very useful, except that I wouldn't entirely agree that
> > the SoRa is "somewhat unique" in doing SIM with confocal sectioning.
> > Hari Shroff and Andy York's iSIM, commercialized by VisiTech quite some
> > time before the SoRa was released, does exactly that too, no?  Needless
> > to say, I am defending my own purchase decision here :-).  And I also
> > think it's useful to point out that there are even more exciting
> > possibilities now by combining Paul Maddox's Mizar TILT with this kind
> > of system, which is what we have just done.  Light sheet and super-res
> > on the same system, easy to combine within a single timelapse
> > experiment?  Exciting times!!!
> >
> > All the best,
> >
> > Alison
> >
> >
> > On 7/18/2019 12:09 PM, samuel connell wrote:
> > > *****
> > > To join, leave or search the confocal microscopy listserv, go to:
> > >
> >
> https://urldefense.proofpoint.com/v2/url?u=http-3A__lists.umn.edu_cgi-2Dbin_wa-3FA0-3Dconfocalmicroscopy&d=DwIBaQ&c=JeTkUgVztGMmhKYjxsy2rfoWYibK1YmxXez1G3oNStg&r=RBx0-WJrAO5vwSOLNmFbqYvikvIZS5ns3-USwvMOuLo&m=MG4gB93r8Ahe6bFz16x4LGVehA_9uWkzEX5Eh3QltPM&s=-I9dYnY9gnXFdJHzix4nDwhcKmaMfCPC8xzSt4dQ7HI&e=
> > > Post images on
> >
> https://urldefense.proofpoint.com/v2/url?u=http-3A__www.imgur.com&d=DwIBaQ&c=JeTkUgVztGMmhKYjxsy2rfoWYibK1YmxXez1G3oNStg&r=RBx0-WJrAO5vwSOLNmFbqYvikvIZS5ns3-USwvMOuLo&m=MG4gB93r8Ahe6bFz16x4LGVehA_9uWkzEX5Eh3QltPM&s=PJ1EvDrAqe26jiSTx4_pVDJfGZLOdaPQ3b5b_XLzeDo&e=
> > and include the link in your posting.
> > > *****
> > >
> > > A quick commercial note. The Yokogawa SoRa is available from a handful
> of
> > > commercial suppliers, including Nikon, 3i, Olympus, Solamere, and
> > Visitron.
> > >
> > > We have found the SoRa to be particularly easy to use and appreciate
> the
> > > flexibility of its integration into a standard spinning disk system.
> > (Quite
> > > straight-forward to add photomanipulation options as well.) Like lots
> of
> > > microscopy systems, one must take great care with the initial system
> > > alignment, but once the system is locked down it is rock solid. The
> core
> > > sensitivity is driven by the camera choice and honestly, different
> camera
> > > options with different S:N capabilities is a nice benefit of the SoRa
> > > design. It's also relatively cost-effective to have a SoRa with a pair
> of
> > > cameras either for simultaneous imaging or for a set of distinct camera
> > > choices. The SoRa is somewhat unique in its capacity to provide
> confocal
> > > optical sectioning with SIM. This is atypical and opens up a wider
> array
> > of
> > > sample choices that are amenable to the SIM technology.
> > >
> > > Feel free to reach out with more specific questions if you'd like.
> > >
> > > --
> > > Sam
> > >
> > > Samuel Connell
> > > Director of Sales
> > >
> > > Intelligent Imaging Innovations (3i)
> > > 3509 Ringsby Court
> > > Denver, CO  80216  USA
> > > 1-720-437-6926
> > > [hidden email]
> > > www.intelligent-imaging.com
> > >
> > > On Wed, Jul 17, 2019 at 5:55 AM <[hidden email]> wrote:
> > >
> > >> *****
> > >> To join, leave or search the confocal microscopy listserv, go to:
> > >>
> >
> https://urldefense.proofpoint.com/v2/url?u=http-3A__lists.umn.edu_cgi-2Dbin_wa-3FA0-3Dconfocalmicroscopy&d=DwIBaQ&c=JeTkUgVztGMmhKYjxsy2rfoWYibK1YmxXez1G3oNStg&r=RBx0-WJrAO5vwSOLNmFbqYvikvIZS5ns3-USwvMOuLo&m=MG4gB93r8Ahe6bFz16x4LGVehA_9uWkzEX5Eh3QltPM&s=-I9dYnY9gnXFdJHzix4nDwhcKmaMfCPC8xzSt4dQ7HI&e=
> > >> Post images on
> >
> https://urldefense.proofpoint.com/v2/url?u=http-3A__www.imgur.com&d=DwIBaQ&c=JeTkUgVztGMmhKYjxsy2rfoWYibK1YmxXez1G3oNStg&r=RBx0-WJrAO5vwSOLNmFbqYvikvIZS5ns3-USwvMOuLo&m=MG4gB93r8Ahe6bFz16x4LGVehA_9uWkzEX5Eh3QltPM&s=PJ1EvDrAqe26jiSTx4_pVDJfGZLOdaPQ3b5b_XLzeDo&e=
> > and include the link in your posting.
> > >> *****
> > >>
> > >> Hello everybody
> > >>
> > >>
> > >> I was wondering if anybody here has had any experience with the Nikon
> > SoRa
> > >> and Zeiss Elyra 7 with lattice SIM.  I know that the two systems
> attain
> > >> super-resolution images in different ways, but just wanted to learn
> > about
> > >> people's experiences with these systems from the point of fast
> super-res
> > >> imaging of live and fixed samples and general attributes, such as
> > >> sensitivity.  Any insights you can share on the subject will be much
> > >> appreciated.
> > >>
> > >>
> > >> Thanks very much in advance,
> > >>
> > >>
> > >> Yevgeniy Romin
> > >>
> > >> =====================================================================
> > >>
> > >>      Please note that this e-mail and any files transmitted from
> > >>      Memorial Sloan Kettering Cancer Center may be privileged,
> > >> confidential,
> > >>      and protected from disclosure under applicable law. If the reader
> > of
> > >>      this message is not the intended recipient, or an employee or
> > agent
> > >>      responsible for delivering this message to the intended
> recipient,
> > >>      you are hereby notified that any reading, dissemination,
> > distribution,
> > >>      copying, or other use of this communication or any of its
> > attachments
> > >>      is strictly prohibited.  If you have received this communication
> > in
> > >>      error, please notify the sender immediately by replying to this
> > >> message
> > >>      and deleting this message, any attachments, and all copies and
> > backups
> > >>      from your computer.
> > >>
> > --
> > Alison J. North, Ph.D.,
> > Research Associate Professor and
> > Senior Director of the Bio-Imaging Resource Center,
> > The Rockefeller University,
> > 1230 York Avenue,
> > New York,
> > NY 10065.
> > Tel: office    ++ 212 327 7488
> > Tel: lab        ++ 212 327 7486
> > Fax:            ++ 212 327 7489
> >
> >
>