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Nikon W1

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Cammer, Michael

Nikon W1

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We would be very interested in emailing or speaking with anyone who has a Nikon W1 running under Elements.

Would greatly appreciate the opportunity to discuss how the system works; would be happy to set up a phone call or Skype.

Thank you!

Michael Cammer, Sr Research Scientist, DART Microscopy Laboratory

NYU Langone Health, 540 First Avenue, SK2 Microscopy Suite, New York, NY 10016

[hidden email]<mailto:[hidden email]> http://nyulmc.org/micros http://microscopynotes.com/

Voice direct only, no text or messages: 1-914-309-3270 and 1-646-501-0567

leavesley

Re: Nikon W1

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Hi Michael,

We're in a similar (not identical) boat. We have an Andor CSU-W1 that
we would like to transition over to control with Elements. We have been
able to switch over almost all of the components to Elements control,
but have been stuck with the Andor ILE laser launch platform, as there
is no option for controlling it with Elements. We would of course be
interested to hear if anyone has ideas about this. If anything we have
done so far to get the other components running with Elements can be
helpful, feel email me directly and we can set up a call. Thanks!

Best regards,

Silas

On 1/3/2020 9:14 AM, Cammer, Michael wrote:

> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> Post images on http://www.imgur.com and include the link in your posting.
> *****
>
> We would be very interested in emailing or speaking with anyone who has a Nikon W1 running under Elements.
>
> Would greatly appreciate the opportunity to discuss how the system works; would be happy to set up a phone call or Skype.
>
> Thank you!
>
> Michael Cammer, Sr Research Scientist, DART Microscopy Laboratory
>
> NYU Langone Health, 540 First Avenue, SK2 Microscopy Suite, New York, NY 10016
>
> [hidden email]<mailto:[hidden email]> http://nyulmc.org/micros http://microscopynotes.com/
>
> Voice direct only, no text or messages: 1-914-309-3270 and 1-646-501-0567

Sylvie Le Guyader

Re: Nikon W1

*****
To join, leave or search the confocal microscopy listserv, go to:
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
Post images on http://www.imgur.com and include the link in your posting.
*****

Hi Silas

We have an X1 running on NIS. But our laser box from Andor is ALC. There is a NIS driver for it. Might be different from the one you have. We have had no problem integrating all other elements but the only Andor parts are the laser box and the cameras.

Med vänlig hälsning / Best regards
Sylvie
@@@@@@@@@@@@@@@@@@@@@@@@
Sylvie Le Guyader, PhD
Live Cell Imaging Facility Manager
Karolinska Institutet- Bionut Dpt
Blickagången 16,
Room 7362 (lab)/7840 (office)
14157 Huddinge, Sweden
mobile: +46 (0) 73 733 5008
LCI website
LCI microscopy blog

-----Original Message-----
From: Confocal Microscopy List <[hidden email]> On Behalf Of Silas Leavesley
Sent: Friday, 3 January, 2020 18:40
To: [hidden email]
Subject: Re: Nikon W1

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Post images on https://eur01.safelinks.protection.outlook.com/?url=http%3A%2F%2Fwww.imgur.com&data=02%7C01%7Csylvie.le.guyader%40KI.SE%7C053dcc79de2e4243b05208d790740269%7Cbff7eef1cf4b4f32be3da1dda043c05d%7C0%7C0%7C637136700248046093&sdata=CUQvAHD5q1J4OEKmDkk1cC8bkO%2BZm2YSX85b47e861M%3D&reserved=0 and include the link in your posting.
*****

Hi Michael,

We're in a similar (not identical) boat. We have an Andor CSU-W1 that we would like to transition over to control with Elements. We have been able to switch over almost all of the components to Elements control, but have been stuck with the Andor ILE laser launch platform, as there is no option for controlling it with Elements. We would of course be interested to hear if anyone has ideas about this. If anything we have done so far to get the other components running with Elements can be helpful, feel email me directly and we can set up a call. Thanks!

Best regards,

Silas

On 1/3/2020 9:14 AM, Cammer, Michael wrote:

> *****
> To join, leave or search the confocal microscopy listserv, go to:
> https://eur01.safelinks.protection.outlook.com/?url=http%3A%2F%2Flists
> .umn.edu%2Fcgi-bin%2Fwa%3FA0%3Dconfocalmicroscopy&data=02%7C01%7Cs
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> cf4b4f32be3da1dda043c05d%7C0%7C0%7C637136700248046093&sdata=tKdFfw
> iaZNpCvtg0ibmAVdvpXtr%2BFdaM93QFMkTFb%2Bg%3D&reserved=0
> Post images on https://eur01.safelinks.protection.outlook.com/?url=http%3A%2F%2Fwww.imgur.com&data=02%7C01%7Csylvie.le.guyader%40KI.SE%7C053dcc79de2e4243b05208d790740269%7Cbff7eef1cf4b4f32be3da1dda043c05d%7C0%7C0%7C637136700248046093&sdata=CUQvAHD5q1J4OEKmDkk1cC8bkO%2BZm2YSX85b47e861M%3D&reserved=0 and include the link in your posting.
> *****
>
> We would be very interested in emailing or speaking with anyone who has a Nikon W1 running under Elements.
>
> Would greatly appreciate the opportunity to discuss how the system works; would be happy to set up a phone call or Skype.
>
> Thank you!
>
> Michael Cammer, Sr Research Scientist, DART Microscopy Laboratory
>
> NYU Langone Health, 540 First Avenue, SK2 Microscopy Suite, New York,
> NY 10016
>
> [hidden email]<mailto:[hidden email]>
> https://eur01.safelinks.protection.outlook.com/?url=http%3A%2F%2Fnyulm
> c.org%2Fmicros&data=02%7C01%7Csylvie.le.guyader%40KI.SE%7C053dcc79
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> 637136700248046093&sdata=V5nUopqLzrg8oLRKzol3%2Fcdy1C1zeQRLlhAKQtS
> 8U68%3D&reserved=0
> https://eur01.safelinks.protection.outlook.com/?url=http%3A%2F%2Fmicro
> scopynotes.com%2F&data=02%7C01%7Csylvie.le.guyader%40KI.SE%7C053dc
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> 0Axs5Sk%3D&reserved=0
>
> Voice direct only, no text or messages: 1-914-309-3270 and
> 1-646-501-0567

--
Silas J. Leavesley, Ph.D.
Professor
Department of Chemical and Biomolecular Engineering Department of Pharmacology Center for Lung Biology University of South Alabama
150 Jaguar Drive, SH4129
Mobile, AL 36688
ph: (251)-460-6160
fax: (251)-461-1485
web: https://eur01.safelinks.protection.outlook.com/?url=http%3A%2F%2Fwww.southalabama.edu%2Fcenters%2Fbioimaging&data=02%7C01%7Csylvie.le.guyader%40KI.SE%7C053dcc79de2e4243b05208d790740269%7Cbff7eef1cf4b4f32be3da1dda043c05d%7C0%7C0%7C637136700248056092&sdata=OEkgRbJBd2ayec6fZYNu0L0u9tI3Oa%2FtuyrTqLs%2Bt3M%3D&reserved=0

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Zdenek Svindrych-2

Re: Nikon W1

In reply to this post by leavesley

*****
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http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
Post images on http://www.imgur.com and include the link in your posting.
*****

Hi Silas,

1) an obvious solution would be direct analog control through NIDAQ, it's
the most flexible and off-the-shelf solution, but it will cost you $$
(NIDAQ card, NIS module, cables). That's what we have and we're happy with
it.

2) Cheaper home-brew solution along the same lines would be a cheap DA
converter that can be controlled with serial (COM) commands, such as
Arduino (https://www.arduino.cc/en/Tutorial/AnalogInOutSerial).
Unfortunately, Arduino Due has only 2 DAC outputs, and the PWM output speed
is quite limited (1 kHz), resulting in slow response (after heavy
filtering).

3) Third, software solution would be direct control of the ILE over serial
(COM) interface, but I don't have the commands description. Provided
they're simple enough, you might be able to figure it out with a COM
traffic sniffer. This document:
http://squishycell.uchicago.edu/manuals/LMM5%20Software%20Manual%20RS232%20Rev%20A.pdf
might
be useful, chances are that LMM5 and ILE use similar control strategy.

Once you have a way to control the ILE with serial commands, you can build
a logical device in NIS Elements and use WritePort() or WritePortEx() or
something like this to control the intensity.

Good luck! Best, zdenek

On Fri, Jan 3, 2020 at 12:40 PM Silas Leavesley <[hidden email]>
wrote:

> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> Post images on http://www.imgur.com and include the link in your posting.
> *****
>
> Hi Michael,
>
> We're in a similar (not identical) boat. We have an Andor CSU-W1 that
> we would like to transition over to control with Elements. We have been
> able to switch over almost all of the components to Elements control,
> but have been stuck with the Andor ILE laser launch platform, as there
> is no option for controlling it with Elements. We would of course be
> interested to hear if anyone has ideas about this. If anything we have
> done so far to get the other components running with Elements can be
> helpful, feel email me directly and we can set up a call. Thanks!
>
> Best regards,
>
> Silas
>
>
> On 1/3/2020 9:14 AM, Cammer, Michael wrote:
> > *****
> > To join, leave or search the confocal microscopy listserv, go to:
> > http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> > Post images on http://www.imgur.com and include the link in your
> posting.
> > *****
> >
> > We would be very interested in emailing or speaking with anyone who has
> a Nikon W1 running under Elements.
> >
> > Would greatly appreciate the opportunity to discuss how the system
> works; would be happy to set up a phone call or Skype.
> >
> > Thank you!
> >
> > Michael Cammer, Sr Research Scientist, DART Microscopy Laboratory
> >
> > NYU Langone Health, 540 First Avenue, SK2 Microscopy Suite, New York,
> NY 10016
> >
> > [hidden email]<mailto:[hidden email]>
> http://nyulmc.org/micros http://microscopynotes.com/
> >
> > Voice direct only, no text or messages: 1-914-309-3270 and
> 1-646-501-0567
> --
> Silas J. Leavesley, Ph.D.
> Professor
> Department of Chemical and Biomolecular Engineering
> Department of Pharmacology
> Center for Lung Biology
> University of South Alabama
> 150 Jaguar Drive, SH4129
> Mobile, AL 36688
> ph: (251)-460-6160
> fax: (251)-461-1485
> web: http://www.southalabama.edu/centers/bioimaging
>

--
--
Zdenek Svindrych, Ph.D.
Research Associate - Imaging Specialist
Department of Biochemistry and Cell Biology
Geisel School of Medicine at Dartmouth

Craig Brideau

Re: Nikon W1

*****
To join, leave or search the confocal microscopy listserv, go to:
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
Post images on http://www.imgur.com and include the link in your posting.
*****

The NI USB-6001 has two analog output channels and is much cheaper than the
PCIe cards. Depending on your control needs, buying two of those might be
more cost effective if you need to control up to four lasers. They are also
USB connected so you can get away with using any computer without having to
worry about internal space for PCIe cards. Note that they are slow (5kS/s),
so if you are trying to do something fancy like end-of-line blanking or
similar they might not work, but if you just need to digitally control the
output of your laser they get the job done. They also provide some digital
IO which can be used for shutters etc.

https://www.ni.com/en-ca/support/model.usb-6001.html

Craig

On Mon, Jan 6, 2020 at 10:43 AM Zdenek Svindrych <[hidden email]> wrote:

> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> Post images on http://www.imgur.com and include the link in your posting.
> *****
>
> Hi Silas,
>
> 1) an obvious solution would be direct analog control through NIDAQ, it's
> the most flexible and off-the-shelf solution, but it will cost you $$
> (NIDAQ card, NIS module, cables). That's what we have and we're happy with
> it.
>
> 2) Cheaper home-brew solution along the same lines would be a cheap DA
> converter that can be controlled with serial (COM) commands, such as
> Arduino (https://www.arduino.cc/en/Tutorial/AnalogInOutSerial).
> Unfortunately, Arduino Due has only 2 DAC outputs, and the PWM output speed
> is quite limited (1 kHz), resulting in slow response (after heavy
> filtering).
>
> 3) Third, software solution would be direct control of the ILE over serial
> (COM) interface, but I don't have the commands description. Provided
> they're simple enough, you might be able to figure it out with a COM
> traffic sniffer. This document:
>
> http://squishycell.uchicago.edu/manuals/LMM5%20Software%20Manual%20RS232%20Rev%20A.pdf
> might
> be useful, chances are that LMM5 and ILE use similar control strategy.
>
> Once you have a way to control the ILE with serial commands, you can build
> a logical device in NIS Elements and use WritePort() or WritePortEx() or
> something like this to control the intensity.
>
> Good luck! Best, zdenek
>
>
> On Fri, Jan 3, 2020 at 12:40 PM Silas Leavesley <
> [hidden email]>
> wrote:
>
> > *****
> > To join, leave or search the confocal microscopy listserv, go to:
> > http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> > Post images on http://www.imgur.com and include the link in your
> posting.
> > *****
> >
> > Hi Michael,
> >
> > We're in a similar (not identical) boat. We have an Andor CSU-W1 that
> > we would like to transition over to control with Elements. We have been
> > able to switch over almost all of the components to Elements control,
> > but have been stuck with the Andor ILE laser launch platform, as there
> > is no option for controlling it with Elements. We would of course be
> > interested to hear if anyone has ideas about this. If anything we have
> > done so far to get the other components running with Elements can be
> > helpful, feel email me directly and we can set up a call. Thanks!
> >
> > Best regards,
> >
> > Silas
> >
> >
> > On 1/3/2020 9:14 AM, Cammer, Michael wrote:
> > > *****
> > > To join, leave or search the confocal microscopy listserv, go to:
> > > http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> > > Post images on http://www.imgur.com and include the link in your
> > posting.
> > > *****
> > >
> > > We would be very interested in emailing or speaking with anyone who has
> > a Nikon W1 running under Elements.
> > >
> > > Would greatly appreciate the opportunity to discuss how the system
> > works; would be happy to set up a phone call or Skype.
> > >
> > > Thank you!
> > >
> > > Michael Cammer, Sr Research Scientist, DART Microscopy Laboratory
> > >
> > > NYU Langone Health, 540 First Avenue, SK2 Microscopy Suite, New York,
> > NY 10016
> > >
> > > [hidden email]<mailto:[hidden email]>
> > http://nyulmc.org/micros http://microscopynotes.com/
> > >
> > > Voice direct only, no text or messages: 1-914-309-3270 and
> > 1-646-501-0567
> > --
> > Silas J. Leavesley, Ph.D.
> > Professor
> > Department of Chemical and Biomolecular Engineering
> > Department of Pharmacology
> > Center for Lung Biology
> > University of South Alabama
> > 150 Jaguar Drive, SH4129
> > Mobile, AL 36688
> > ph: (251)-460-6160
> > fax: (251)-461-1485
> > web: http://www.southalabama.edu/centers/bioimaging
> >
>
>
> --
> --
> Zdenek Svindrych, Ph.D.
> Research Associate - Imaging Specialist
> Department of Biochemistry and Cell Biology
> Geisel School of Medicine at Dartmouth
>