Search the CONFOCAL archive at
http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocalPutting a lens between the scanhead and the microscope will change the
effective size of the pinholes as seen by the objective. Depending on
the objective you're using and the pinhole size this may be a good or a
bad thing.
Mounting the lens between the scanhead and the camera should affect only
the magnification of the image on the camera without changing anything else.
There are probably other differences between the two lens positions;
I'll leave comments on that for someone more knowledgeable than myself.
Kurt
David Knecht wrote:
> Search the CONFOCAL archive at
>
http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal We recently
> demo'd several spinning disk confocal systems. For Nyquist matching
> of camera and objective, we saw two different solutions. In one case,
> a lens was mounted between the scan head and the microscope so that
> both excitation and emission went through it. In the other case, it
> was mounted between the scan head and the camera so only emission went
> through. Can anyone comment on the relative merits of this design
> decision? THanks- Dave
>
> Dr. David Knecht
> Department of Molecular and Cell Biology
> Co-head Flow Cytometry and Confocal Microscopy Facility
> U-3125
> 91 N. Eagleville Rd.
> University of Connecticut
> Storrs, CT 06269
> 860-486-2200
> 860-486-4331 (fax)
>
>
--
Kurt Thorn, PhD
Director, Nikon Imaging Center
University of California San Francisco
UCSF MC 2140
Genentech Hall Room S252
600 16th St.
San Francisco, CA 94158-2517
http://nic.ucsf.eduphone 415.514.9709
fax 415.514.4300
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