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Hi all
I am doing 2-channel 3D SIM on an OMX Blaze system, and I discovered something really unhelpful. I was tuning the acquisition settings to try to get the 2-channel z-stack within 7.5 seconds, and from the timestamps on the resulting stack thought I had found a good balance of exposure time, % transmission and number of sections. So then I set up my experiment to image some time points, but after feeling it took too long I manually timed it.
The metadata in the image states that the last z/c/t is around 1m20s, but in reality it took nearly 4 minutes to acquire this. So all of the time stamps in the image are wrong. It would appear that the time stamps in the image are the cumulative 'light-time' at each point. When you're trying to time a biological process this is really not a useful metric.
Has anyone encountered this and found a work around? Even better, is the correct time stamp stored elsewhere in the metadata?
Many thanks,
Michael.
Michael Porter
Centre for Gene Regulation and Expression
College of Life Sciences
University of Dundee
Dow Street
Dundee
DD1 5EH
UK
The University of Dundee is a registered Scottish Charity, No: SC015096
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