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Objective storage?

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Adrian Smith-6

Objective storage?

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Hi all,

Slightly off-topic but I'm interested in how people store their objectives.

In particular if anyone knows where to buy empty containers/cases/boxes to hold loose objectives?

Some of our objectives came in plastic containers with threads in the lids to screw the objectives into... but some did not and I'm not sure what is the best way to handle storing them.

Regards,

Adrian Smith
Centenary Institute, Sydney, Australia

SL Smith

Re: Objective storage?

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Ask your local reps for more containers. That should be no problem. Since many
of the mounts are standard threads, it doesn't even have to be the same
company.

For water immersion objectives used in experiments in live tissue, storing them
with a bit of water in the bottom of the container is a good way to keep them
from growing stubborn salt crystals. As shown here:
http://labrigger.com/blog/2010/07/15/cleaning-objectives-and-other-optics/

Larson Jeffrey M.

Re: Objective storage?

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Leaving objective lenses in a humid environment is a good way to
encourage growth of mold, trading one problem for another. I believe it
wiser to remove the water immersion objective lens from the microscope
after use and clean it carefully with a gentle water based cleaning
solution like 'Sparkle'. Make sure that the lens is dry and store it in
its original capsule. Be sure to put the nosepiece plug back in the
nosepiece. Many high numerical aperture dipping lenses do not have the
same thread sizes as typical lenses from their manufacturers. Your rep
should provide you with a replacement capsule identical to the one your
objective lens was delivered in if you've lost yours. You'll also want
to ask for a replacement nosepiece plug, or maybe a few of them.

Jeffrey M Larson
Confocal Systems Product Manager

Nikon Instruments Inc.
1300 Walt Whitman Road
Melville NY 11747-3064

Office: 631-547-8540 Fax: 631-547-4033
Mobile: 516-617-2228
[hidden email] www.nikoninstruments.com
-----Original Message-----
From: Confocal Microscopy List [mailto:[hidden email]]
On Behalf Of SL Smith
Sent: Friday, September 24, 2010 5:07 AM
To: [hidden email]
Subject: Re: Objective storage?

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Ask your local reps for more containers. That should be no problem.
Since many
of the mounts are standard threads, it doesn't even have to be the same
company.

For water immersion objectives used in experiments in live tissue,
storing them
with a bit of water in the bottom of the container is a good way to keep
them
from growing stubborn salt crystals. As shown here:
http://labrigger.com/blog/2010/07/15/cleaning-objectives-and-other-optic
s/

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Re: Objective storage?

In reply to this post by Adrian Smith-6

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Dear Adrian,
if it's about zeiss lenses just let me know the Order numbers printed on the lenses and we will take care

Best regards
Bruene Venus
Product Manager with Carl Zeiss MicroImaging in Germany

Von meinem iPhone gesendet

Am 24.09.2010 um 06:17 schrieb Adrian Smith <[hidden email]>:

> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> *****
>
> Hi all,
>
> Slightly off-topic but I'm interested in how people store their objectives.
>
> In particular if anyone knows where to buy empty containers/cases/boxes to hold loose objectives?
>
> Some of our objectives came in plastic containers with threads in the lids to screw the objectives into... but some did not and I'm not sure what is the best way to handle storing them.
>
> Regards,
>
> Adrian Smith
> Centenary Institute, Sydney, Australia

Guy Cox-2

Re: Objective storage?

In reply to this post by Adrian Smith-6

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The trouble with storing objectives in the manufacturers' containers is
that it is then natural to stand them on the lid (the widest part). But
immersion objectives should always be stored pointing down so that
nothing can run into the lens. We make up racks to hold the containers
so that they have to be put in that way up. We've also had some lenses
supplied in cardboard boxes with no proper container, but mostly these
were cheap 'lab' or 'student' objectives which weren't so important. In
such cases you have to decide whether the objective is worth the cost of
a case.

Guy

Optical Imaging Techniques in Cell Biology
by Guy Cox CRC Press / Taylor & Francis
http://www.guycox.com/optical.htm
______________________________________________
Associate Professor Guy Cox, MA, DPhil(Oxon)
Australian Centre for Microscopy & Microanalysis,
Madsen Building F09, University of Sydney, NSW 2006

Phone +61 2 9351 3176 Fax +61 2 9351 7682
Mobile 0413 281 861
______________________________________________
http://www.guycox.net

-----Original Message-----
From: Confocal Microscopy List [mailto:[hidden email]]
On Behalf Of Adrian Smith
Sent: Friday, 24 September 2010 2:17 PM
To: [hidden email]
Subject: Objective storage?

*****
To join, leave or search the confocal microscopy listserv, go to:
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
*****

Hi all,

Slightly off-topic but I'm interested in how people store their
objectives.

In particular if anyone knows where to buy empty containers/cases/boxes
to hold loose objectives?

Some of our objectives came in plastic containers with threads in the
lids to screw the objectives into... but some did not and I'm not sure
what is the best way to handle storing them.

Regards,

Adrian Smith
Centenary Institute, Sydney, Australia

Kurt Thorn

Re: Objective storage?

In reply to this post by Adrian Smith-6

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We've designed an objective case that can be 3D-printed - you can
download a model of it here: http://derisilab.ucsf.edu/index.php?3D=94

We've had some problems with storing the objectives in the
manufacturer's cases as the plastic erodes into the objective threads
and causes the objectives to stick in the microscope turret.

Kurt

On 9/23/2010 9:17 PM, Adrian Smith wrote:

Cameron Nowell

Re: Objective storage?

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Hi Kurt,

Your vase stores the objectives upside down. like Guy suggested it is best to store oil objectives pointing down to stop oil from seeping down inside the objective over the years. Are the objectives held in place somehow so you could invert the case if required?

Cheers

Cam

Cameron J. Nowell
Microscpy Manager
Central Resource for Advanced Microscopy
Ludwig Insttue for Cancer Research
PO Box 2008
Royal Melbourne Hospital
Victoria, 3050
AUSTRALIA

Office: +61 3 9341 3155
Mobile: +61422882700
Fax: +61 3 9341 3104

http://www.ludwig.edu.au/branch/research/platform/microscopy.htm

________________________________

From: Confocal Microscopy List on behalf of Kurt Thorn
Sent: Tue 28/09/2010 3:00 AM
To: [hidden email]
Subject: Re: Objective storage?

*****
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We've designed an objective case that can be 3D-printed - you can
download a model of it here: http://derisilab.ucsf.edu/index.php?3D=94

We've had some problems with storing the objectives in the
manufacturer's cases as the plastic erodes into the objective threads
and causes the objectives to stick in the microscope turret.

Kurt

On 9/23/2010 9:17 PM, Adrian Smith wrote:

Kurt Thorn

Re: Objective storage?

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No, they just sit in there. We've had more trouble with objective
threads getting gummed up with plastic particles than with oil running
into the objectives (fingers crossed!) so we explicitly didn't want the
objectives to thread into anything. For other projects we've tried to
tap the ABS our 3D printer prints without much success.

Kurt

On 9/27/2010 3:18 PM, Cameron Nowell wrote:

> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> *****
>
> Hi Kurt,
>
> Your vase stores the objectives upside down. like Guy suggested it is best to store oil objectives pointing down to stop oil from seeping down inside the objective over the years. Are the objectives held in place somehow so you could invert the case if required?
>
>
> Cheers
>
>
> Cam
>
>
> Cameron J. Nowell
> Microscpy Manager
> Central Resource for Advanced Microscopy
> Ludwig Insttue for Cancer Research
> PO Box 2008
> Royal Melbourne Hospital
> Victoria, 3050
> AUSTRALIA
>
> Office: +61 3 9341 3155
> Mobile: +61422882700
> Fax: +61 3 9341 3104
>
> http://www.ludwig.edu.au/branch/research/platform/microscopy.htm
>
>
> ________________________________
>
> From: Confocal Microscopy List on behalf of Kurt Thorn
> Sent: Tue 28/09/2010 3:00 AM
> To: [hidden email]
> Subject: Re: Objective storage?
>
>
>
> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> *****
>
> We've designed an objective case that can be 3D-printed - you can
> download a model of it here: http://derisilab.ucsf.edu/index.php?3D=94
>
> We've had some problems with storing the objectives in the
> manufacturer's cases as the plastic erodes into the objective threads
> and causes the objectives to stick in the microscope turret.
>
> Kurt
>
> On 9/23/2010 9:17 PM, Adrian Smith wrote:
>> *****
>> To join, leave or search the confocal microscopy listserv, go to:
>> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
>> *****
>>
>> Hi all,
>>
>> Slightly off-topic but I'm interested in how people store their objectives.
>>
>> In particular if anyone knows where to buy empty containers/cases/boxes to hold loose objectives?
>>
>> Some of our objectives came in plastic containers with threads in the lids to screw the objectives into... but some did not and I'm not sure what is the best way to handle storing them.
>>
>> Regards,
>>
>> Adrian Smith
>> Centenary Institute, Sydney, Australia
>>