Oil in a 10x objective
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Can anyone come up with a plausible explanation as to how oil got
into the 10x objective on our LSM510 with an upright stand? Yesterday someone complained about poor imaging through the objective, and I discovered that there was a bubble of oil trapped between internal elements. I'm at a loss as to how someone got oil inside this low mag, non-immersion lens. Any ideas? Steve... -- ____________________________________________________________________________ Steven E. Ruzin, Ph.D. Director, Biological Imaging Facility Curator, Golub Microscope Collection 381 Koshland Hall College of Natural Resources University of California Berkeley CA 94720-3102 510-642-6602 510-642-4995 (fax) http://golubcollection.berkeley.edu http://microscopy.berkeley.edu |
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A great wise one once told me to always store objectives in their case
with back aperture up, so just in case oil didn't drip into the body if stored back aperture down. Best, Gary Laevsky, Ph.D. Imaging Application Specialist Andor Technology discover new ways of seeing [hidden email] Cell (774) 291 - 9992 Office (860) 290 - 9211 x219 Fax (860) 290 - 9566 Web: www.andor.com -----Original Message----- From: Confocal Microscopy List [mailto:[hidden email]] On Behalf Of Steven Ruzin Sent: Friday, March 27, 2009 2:14 PM To: [hidden email] Subject: Oil in a 10x objective Can anyone come up with a plausible explanation as to how oil got into the 10x objective on our LSM510 with an upright stand? Yesterday someone complained about poor imaging through the objective, and I discovered that there was a bubble of oil trapped between internal elements. I'm at a loss as to how someone got oil inside this low mag, non-immersion lens. Any ideas? Steve... -- ________________________________________________________________________ ____ Steven E. Ruzin, Ph.D. Director, Biological Imaging Facility Curator, Golub Microscope Collection 381 Koshland Hall College of Natural Resources University of California Berkeley CA 94720-3102 510-642-6602 510-642-4995 (fax) http://golubcollection.berkeley.edu http://microscopy.berkeley.edu |
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In reply to this post by Steve Ruzin
User focusing on specimen with a 63x/1.4 oil objective, user then
chooses 10x/0.5 from dropdown list in software and depresses the button, stage drops, nosepiece quickly rotates into position, stage raises, user then focuses 10x/0.5 objective into immersion oil, user does not bother to clean oil off of the 10x or tell core manager, core manager does not bother to check if oil is on the 10x because no-one would put oil on a 10x, right? Oil remains for days/weeks, due to capillary action oil invades the elements of the objective. Oil is now inside. It's just that easy. Or, perhaps I am completely wrong and it was by magic. Cheers, Brian -----Original Message----- From: Confocal Microscopy List [mailto:[hidden email]] On Behalf Of Steven Ruzin Sent: Friday, March 27, 2009 11:14 AM To: [hidden email] Subject: Oil in a 10x objective Can anyone come up with a plausible explanation as to how oil got into the 10x objective on our LSM510 with an upright stand? Yesterday someone complained about poor imaging through the objective, and I discovered that there was a bubble of oil trapped between internal elements. I'm at a loss as to how someone got oil inside this low mag, non-immersion lens. Any ideas? Steve... -- ________________________________________________________________________ ____ Steven E. Ruzin, Ph.D. Director, Biological Imaging Facility Curator, Golub Microscope Collection 381 Koshland Hall College of Natural Resources University of California Berkeley CA 94720-3102 510-642-6602 510-642-4995 (fax) http://golubcollection.berkeley.edu http://microscopy.berkeley.edu --------------------------------------------------------------------- SECURITY/CONFIDENTIALITY WARNING: This message and any attachments are intended solely for the individual or entity to which they are addressed. This communication may contain information that is privileged, confidential, or exempt from disclosure under applicable law (e.g., personal health information, research data, financial information). Because this e-mail has been sent without encryption, individuals other than the intended recipient may be able to view the information, forward it to others or tamper with the information without the knowledge or consent of the sender. If you are not the intended recipient, or the employee or person responsible for delivering the message to the intended recipient, any dissemination, distribution or copying of the communication is strictly prohibited. If you received the communication in error, please notify the sender immediately by replying to this message and deleting the message and any accompanying files from your system. If, due to the security risks, you do not wish to receive further communications via e-mail, please reply to this message and inform the sender that you do not wish to receive further e-mail from the sender. --------------------------------------------------------------------- |
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In reply to this post by Steve Ruzin
Are you sure it's oil and not some other fluid? Either way, send the
lens in for servicing, don't try to clean it yourself. Andy At 01:13 PM 3/27/2009, you wrote: >Can anyone come up with a plausible explanation as to how oil got >into the 10x objective on our LSM510 with an upright stand? >Yesterday someone complained about poor imaging through the >objective, and I discovered that there was a bubble of oil trapped >between internal elements. I'm at a loss as to how someone got oil >inside this low mag, non-immersion lens. Any ideas? > >Steve... >-- >____________________________________________________________________________ >Steven E. Ruzin, Ph.D. >Director, Biological Imaging Facility >Curator, Golub Microscope Collection >381 Koshland Hall College of Natural Resources >University of California Berkeley CA 94720-3102 >510-642-6602 510-642-4995 (fax) >http://golubcollection.berkeley.edu >http://microscopy.berkeley.edu Andrew Resnick, Ph. D. Instructor Department of Physiology and Biophysics Case Western Reserve University 216-368-6899 (V) 216-368-4223 (F) |
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>Are you sure it's oil and not some other fluid? Either way, send the
>lens in for servicing, don't try to clean it yourself. > Not sure, but it probably is. I wouldn't try to clean it myself. -- ____________________________________________________________________________ Steven E. Ruzin, Ph.D. Director, Biological Imaging Facility 381 Koshland Hall College of Natural Resources University of California Berkeley CA 94720-3102 510-642-6602 510-642-4995 (fax) http://microscopy.berkeley.edu |
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In reply to this post by Gary Laevsky-2
All our objective bottles actually store them this way. The lens screws into the lid of the bottle and points down.
Craig On Fri, Mar 27, 2009 at 12:22 PM, Gary Laevsky <[hidden email]> wrote: A great wise one once told me to always store objectives in their case |
 
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Goodhouse, Joseph G. |
Re: Oil in a 10x objective
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In reply to this post by Steve Ruzin
In order for the fluid to get into your lens you would also have
to have the lens seal compromised. Now how does that happen? I once had a user drop a 40X plan Apo 1.3 oil objective. The impact put a dent in the side of the objective and it cracked the optic. Needless to say whoever did it did not come forward. Cleaning lenses with organics such as acetone will compromise the sealant. I have seen this occur. Joe Goodhouse Confocal Core Lab Manager Dept. of Molecular Biology Princeton University 609-258-5432 Visit us at http://www.molbio1.princeton.edu/facility/confocal/ -----Original Message----- From: Confocal Microscopy List [mailto:[hidden email]] On Behalf Of Steven Ruzin Sent: Friday, March 27, 2009 2:14 PM To: [hidden email] Subject: Oil in a 10x objective Can anyone come up with a plausible explanation as to how oil got into the 10x objective on our LSM510 with an upright stand? Yesterday someone complained about poor imaging through the objective, and I discovered that there was a bubble of oil trapped between internal elements. I'm at a loss as to how someone got oil inside this low mag, non-immersion lens. Any ideas? Steve... -- ________________________________________________________________________ ____ Steven E. Ruzin, Ph.D. Director, Biological Imaging Facility Curator, Golub Microscope Collection 381 Koshland Hall College of Natural Resources University of California Berkeley CA 94720-3102 510-642-6602 510-642-4995 (fax) http://golubcollection.berkeley.edu http://microscopy.berkeley.edu |
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Goodhouse, Joseph G. |
Re: Oil in a 10x objective
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In reply to this post by Craig Brideau
Storing lenses. Recently I was informed by one of my
microscope technical people that the proper way to store optics is the same way
the are mounted on your microscope. That is lens down for an upright
microscope and lens up for an inverted microscope. This is to prevent
immersion fluids from getting into the lens ( around the fluid barriers)
by gravitational and capillary action.
Joe Goodhouse
Visit us at http://www.molbio1.princeton.edu/facility/confocal/ From: Confocal Microscopy List [mailto:[hidden email]] On Behalf Of Craig Brideau Sent: Friday, March 27, 2009 3:00 PM To: [hidden email] Subject: Re: Oil in a 10x objective Craig On Fri, Mar 27, 2009 at 12:22 PM, Gary Laevsky <[hidden email]> wrote: A great wise one once told me to always store objectives in their case |
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In reply to this post by Armstrong, Brian
We have the same event happening the same way; most users are very honest about it and this gives me a chance to clean the 10x quickly. (I do clean all lenses anyway 1-2x a week). No permanent damage so far, knock on wood!
Zoltan On Fri, Mar 27, 2009 at 6:47 PM, Armstrong, Brian <[hidden email]> wrote: User focusing on specimen with a 63x/1.4 oil objective, user then -- Zoltan Cseresnyes Facility manager, Imaging Suite |
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In reply to this post by Steve Ruzin
Steve
This is not an answer but it might put a smile on your face, if you have a sick sense of humor. When I serviced Zeiss microscopes, in a former life, I had a client at a major University that asked to have their "immersion scope" serviced. When I got there I found that a foreign speaking student had poured a whole bottle of immersion oil into the support arm of the Zeiss Photo III microscope. If his English comprehension would have been a bit better, it would not have happened. It was a mess. The camera mechanism, connectors, optics, and PMT was soaked. I hope the repair cost of your 10X is not anywhere near the cost they had to endure! Dan On Mar 27, 2009, at 2:13 PM, Steven Ruzin wrote:
Dan Focht Bioptechs 724-282-7145 www.bioptechs.com |
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>Steve
> > >This is not an answer but it might put a smile on your face, if you >have a sick sense of humor. > >When I serviced Zeiss microscopes, in a former life, I had a client >at a major University that asked to have their "immersion scope" >serviced. >When I got there I found that a foreign speaking student had poured >a whole bottle of immersion oil into the support arm of the Zeiss >Photo III microscope. >If his English comprehension would have been a bit better, it would >not have happened. >It was a mess. The camera mechanism, connectors, optics, and PMT was soaked. > >I hope the repair cost of your 10X is not anywhere near the cost >they had to endure! I might just take a look down the eyepiece mount. This, btw, is a recurring legend. One I've heard before. You are the first I've heard of who actually experienced it. Steve... -- ____________________________________________________________________________ Steven E. Ruzin, Ph.D. Director, Biological Imaging Facility 381 Koshland Hall College of Natural Resources University of California Berkeley CA 94720-3102 510-642-6602 510-642-4995 (fax) http://microscopy.berkeley.edu |
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Couldn't resist adding this story -
Years ago, a student from another lab wanted to use our inverted microscope to image cells. It was all explained carefully, that day she looked at her slides under supervision, seemed to be doing all correctly. My PhD student watched her in her next session the next day, all seemed fine. Then she worked for the next 2-3 days on her material, finally saying she was having problems imaging her cells, though it had been good at the start. Had a look down the scope, horrible image indeed, because.... Instead of using the 100x oil, she had (for unknown reasons) been using the 10x dry objective, but trying to use it with oil. Since the images were so poor, and she had a hard time getting the oil to touch the coverslip, she kept adding more and more oil, so apart from the objective being covered, there was oil down in the nosepiece, everywhere. And there was the broken end of a glass pipette sitting in the concave tip of the objective, despite being trained to only use the plastic pipettes, and despite there being no glass pipettes in the room - she'd brought in her own, preferring them to plastic!! After cleaning, objective was unharmed, nosepiece clean, but it taught me a lesson about keeping a close eye on novices! And the student? After finishing her Hons degree, didn't go on in science.... cheers, Rosemary On 28/03/09 10:22 AM, "Steven Ruzin" <[hidden email]> wrote: >> Steve >> >> >> This is not an answer but it might put a smile on your face, if you >> have a sick sense of humor. >> >> When I serviced Zeiss microscopes, in a former life, I had a client >> at a major University that asked to have their "immersion scope" >> serviced. >> When I got there I found that a foreign speaking student had poured >> a whole bottle of immersion oil into the support arm of the Zeiss >> Photo III microscope. >> If his English comprehension would have been a bit better, it would >> not have happened. >> It was a mess. The camera mechanism, connectors, optics, and PMT was soaked. >> >> I hope the repair cost of your 10X is not anywhere near the cost >> they had to endure! > > I might just take a look down the eyepiece mount. > > This, btw, is a recurring legend. One I've heard before. You are the > first I've heard of who actually experienced it. > > Steve... |
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>Couldn't resist adding this story -
> >Years ago, a student from another lab wanted to use our inverted microscope >to image cells. It was all explained carefully, that day she looked at her >slides under supervision, seemed to be doing all correctly. My PhD student >watched her in her next session the next day, all seemed fine. > >Then she worked for the next 2-3 days on her material, finally saying she >was having problems imaging her cells, though it had been good at the start. >Had a look down the scope, horrible image indeed, because.... Instead of >using the 100x oil, she had (for unknown reasons) been using the 10x dry >objective, but trying to use it with oil. Since the images were so poor, >and she had a hard time getting the oil to touch the coverslip, she kept >adding more and more oil, so apart from the objective being covered, there >was oil down in the nosepiece, everywhere. And there was the broken end of >a glass pipette sitting in the concave tip of the objective, despite being >trained to only use the plastic pipettes, and despite there being no glass >pipettes in the room - she'd brought in her own, preferring them to >plastic!! > >After cleaning, objective was unharmed, nosepiece clean, but it taught me a >lesson about keeping a close eye on novices! And the student? After >finishing her Hons degree, didn't go on in science.... > Well, that's a possibility here. While the condenser is often oily, last week it was positively soaked. It's possible that I have a repeat of your problem in my lab. Steve... -- ____________________________________________________________________________ Steven E. Ruzin, Ph.D. Director, Biological Imaging Facility 381 Koshland Hall College of Natural Resources University of California Berkeley CA 94720-3102 510-642-6602 510-642-4995 (fax) http://microscopy.berkeley.edu |
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In reply to this post by Steve Ruzin
I've had a few air objectives need to go back to Zeiss for 'repair', but
generally it always seems to be cell culture fluid or similar, and that's nasty when it dries up on internal optics - most of our microscopes are inverted and so will be at greater risk [gravity]. Non drying immersion oil has just crept into our Zeiss 40x Plan Neofluar top lens but at the moment it images OK, but Zeiss say it's not economical to repair, i.e. it's a lot more than the ~60% [of new] cost of repairs I paid last-time, about 4 years ago. I've never decided whether it's better to fully clean the oil objective before storage - do you let the oil or the solvents do the damage? I tend to never solvent clean the oil off if it's in frequent use. [manufacturers do at least test the oil for safe use on objectives]. Given the deep scratches the users leave on the objectives it probably doesn't matter either way. Although oil creeps downwards [and upwards] I doubt it could make it to the back of the objective [assuming its top's been wiped]. In my experience it's temporarily resting the objective on a desktop that's contaminated with oil [users get it everywhere] that does that. You often can't see the spilt oil. I always put folded tissue down if I have to rest the objective somewhere, but mostly I try to keep it my hand and put it straight into it's plastic 'tube' [to avoid the oil contamination - tried it once, didn't like it]. Pushing down the sprung top of the oil objective can push oil past the o ring seal from where it can creep downwards unseen on the inner tube [not into the internal objective optics, but it can drip into the microscope body underneath [a problem with inverted microscopes]. So I advise users to avoid pushing the oil objective top down. Carefully apply the single small immersion oil drop to the objective lens never the slide, particularly with inverted microscopes. Once the 'liquid's' inside an objective you can't do much about it, other than get more liquid inside when 'cleaning'. This does remind to replace my Cargille DF oil, it's been open over 4 years and we should have replaced after 2.5 years. It's that oil acidity again. Keith --------------------------------------------------------------------------- Dr Keith J. Morris, Molecular Cytogenetics and Microscopy Core, Laboratory 00/069 and 00/070, The Wellcome Trust Centre for Human Genetics, Roosevelt Drive, Oxford OX3 7BN, United Kingdom. Telephone: +44 (0)1865 287568 Email: [hidden email] Web-pages: http://www.well.ox.ac.uk/cytogenetics/ -----Original Message----- From: Confocal Microscopy List [mailto:[hidden email]] On Behalf Of Steven Ruzin Sent: 27 March 2009 18:57 To: [hidden email] Subject: Re: Oil in a 10x objective >Are you sure it's oil and not some other fluid? Either way, send the >lens in for servicing, don't try to clean it yourself. > Not sure, but it probably is. I wouldn't try to clean it myself. -- ____________________________________________________________________________ Steven E. Ruzin, Ph.D. Director, Biological Imaging Facility 381 Koshland Hall College of Natural Resources University of California Berkeley CA 94720-3102 510-642-6602 510-642-4995 (fax) http://microscopy.berkeley.edu |
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