On Sale: Leica DMI3000B fluorescent semi-automatic inverted wide-field microscope with OptGrid for "confocal-like pictures and Volocity software for acquisition and quantification

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Mirco Martino Mirco Martino
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On Sale: Leica DMI3000B fluorescent semi-automatic inverted wide-field microscope with OptGrid for "confocal-like pictures and Volocity software for acquisition and quantification

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Hello all,

because we are buying a new microscope system, we are selling our current microscope with all components that make it a fluorescent semi-automatic wide-field inverted microscope that can be controlled for automatic acqusition by Volocity software. The system has also the OptiGrid system that permits to have "confocal-like" pictures.

http://edge.rit.edu/edge/P09052/public/OptiGrid%20Theory (OptiGrid Theory explanation)

Price: ~60.000 SEK
 
Located in Karolinska Institutet, Solna, Sweden

Contact me in private for more information or if interested: [hidden email]

Fluorescent Inverted Microscope with OptiGrid, structured illumination system that enables confocal-like pictures.

The xy stage, the z driver and the acquisition parameters (filters, expsorue time etc.) can be automatic setted and controlled by the Volocity Software. The software can be utilized also for process the pictures with the OptiGrid algorithm and to make measurements like intensity or distances. Software for acquisition and measurements: Volocity 5.5 (Perkin Elmer). Company: Leica Microsystems CMS GmbH Model: DMI3000 B

https://drive.google.com/file/d/1NuCfp_5SwcfMM9GBO4yAsg1w0AIWKBH3/view 

https://www.leica-microsystems.com/fileadmin/downloads/Leica%20DMI3000%20B/Brochures/Leica_DMI3000_B-Brochure_en.pdf 

Objectives:
o HCX APO 63X/1,40 Oil PH3 CS
o HC PL APO 20X/0,70 H1l/02 IMM CORR CS: ring to correct immersion medium (Oil or Glycerol) or numerical aperture (0,17-W-0)
o HCX PL APO 100X/1,40-0,70 Oil
o HCX PL FLOTAR 40X/0,75 PH2
o HI PLAN 1 10X/0,22 PH1

Camera: CCD Orca-R2 Hamamatsu C10600 with:
o Quantum efficiency: over 70%
o Read Noise: 6 electrons (Normal Scan), 10 electrons (Fast Scan)
o Pixel Size: 6,45 μm

Excitation and Emission filters and dichroic mirrors are part of Angstrom Filters ANG 0812008 and controlled by Spectral Laser Change device

Excitations Filters (All Bandwidth):
o Semrock FF390/40X
o Chroma ET430/24X
o Chroma ET490/20X
o Chroma ET500/20X
o Chroma ET546/10X
o Chroma ET555/25X
o Chroma ET590/20X
o Chroma ET640/30X
o Chroma ET645/30X
o Chroma ET740/40X

Dichroic Mirrors:
o Chroma DAPI/Aqua/Green/Texas Red
o Chroma Cy3/Cy5/Cy7
o Chroma 89000 SEDAT QUAD

Emission Filters (All Bandwidth):
o Chroma ET445/30M
o Chroma ET455/56M
o Chroma ET470/17M
o Chroma ET530/20M
o Chroma ET570/17M
o Chroma ET625/30M
o Chroma ET632/60M
o Chroma ET680/50M
o Chroma ET705/72M
o Chroma ET800/50M

Two Illumination systems based on Mercury Lamps
• One for the eyepieces: Prior Lumen200
• One for the camera: X-cute Exacte, Model XCT10

Ludl Mac 6000 system for automatized computer-controlled functions with: focus motor drive, shutter control, communication control

Optigrid Structured Illuminated System from Qioptiimaging

ProScan III Prior control and precision motorized stage

Volocity Software 5.5 with packing for acquisition, optigrid communication and post-acquisition analysis