Onix Microfluidics Perfusion System

[Cameron Nowell]

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Hi List,

 

Has anyone out there had any experience with the Onix Perfusion system?

 

http://www.cellasic.com/ONIX.html

 

I have a lab interested in growing organoids in gradients of various
growth factors, cytokines etc. And they are keen to have this system. I
wooud greatly appreciate any insight as to how it actually performs.

 

 

Cheers

 

Cam

 

 

 

Cameron J. Nowell
Microscopy Manager
Centre for Advanced Microscopy
Ludwig Institute for Cancer Research
PO Box 2008
Royal Melbourne Hospital
Victoria, 3050
AUSTRALIA

Office: [phone]
Mobile: [phone]
Fax: [phone]

Facility Website <http://www.ludwig.edu.au/confocal/>

 

 



This communication is intended only for the named recipient and may contain information that is confidential, legally privileged or subject to copyright; the Ludwig Institute for Cancer Research Ltd does not waive any rights if you have received this communication in error.
The views expressed in this communication are those of the sender and do not necessarily reflect the views of the Ludwig Institute for Cancer Research Ltd.

[Graham Wright]

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Hi Cam,

My old institute had one, and whilst I did not directly use it the lab
working with it had a good experience. They were growing fission yeast (S.
pombe) cells and were using the combination of flow and thinning/sloping
sample chamber to fix the cells in position then alter their environment
with the perfusion system whilst monitoring rate of division. As pombe cells
are pretty much uniform in their size and shape and not adherent they got
wedged in the sample chamber at consistent positions, however I don't know
if this would be applicable for organoids.

Regards,
Graham

--
*Graham Wright, PhD*
Microscopy Unit Manager

Institute of Medical Biology
8A Biomedical Grove, #06-06 Immunos, Singapore 138648

O:   [phone]
E:   [hidden email]
W:  http://www.imb.a-star.edu.sg/imu/


On 15 June 2011 14:58, Cameron Nowell <[hidden email]> wrote:

> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> *****
>
> Hi List,
>
>
>
> Has anyone out there had any experience with the Onix Perfusion system?
>
>
>
> http://www.cellasic.com/ONIX.html
>
>
>
> I have a lab interested in growing organoids in gradients of various
> growth factors, cytokines etc. And they are keen to have this system. I
> wooud greatly appreciate any insight as to how it actually performs.
>
>
>
>
>
> Cheers
>
>
>
> Cam
>
>
>
>
>
>
>
> Cameron J. Nowell
> Microscopy Manager
> Centre for Advanced Microscopy
> Ludwig Institute for Cancer Research
> PO Box 2008
> Royal Melbourne Hospital
> Victoria, 3050
> AUSTRALIA
>
> Office: [phone]
> Mobile: [phone]
> Fax: [phone]
>
> Facility Website <http://www.ludwig.edu.au/confocal/>
>
>
>
>
>
>
>
> This communication is intended only for the named recipient and may contain
> information that is confidential, legally privileged or subject to
> copyright; the Ludwig Institute for Cancer Research Ltd does not waive any
> rights if you have received this communication in error.
> The views expressed in this communication are those of the sender and do
> not necessarily reflect the views of the Ludwig Institute for Cancer
> Research Ltd.
>

[George McNamara]

*****
To join, leave or search the confocal microscopy listserv, go to:
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
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Hi Cam,

We had a seminar and demo last year - the controller is great, the
microperfusion plates come in lots of varieties, very impressive when
they work - just like the many videos on their web site. The only
problem is that a lot of the demo plates developed bubbles in the lines
during shipping each of those plates useless. Hopefully CellASIC has
resolved this problem by now.

George
p.s. they also have a line of plates that work by gravity - no
controller needed.


On 6/15/2011 2:58 AM, Cameron Nowell wrote:

> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> *****
>
> Hi List,
>
>
>
> Has anyone out there had any experience with the Onix Perfusion system?
>
>
>
> http://www.cellasic.com/ONIX.html
>
>
>
> I have a lab interested in growing organoids in gradients of various
> growth factors, cytokines etc. And they are keen to have this system. I
> wooud greatly appreciate any insight as to how it actually performs.
>
>
>
>
>
> Cheers
>
>
>
> Cam
>
>
>
>
>
>
>
> Cameron J. Nowell
> Microscopy Manager
> Centre for Advanced Microscopy
> Ludwig Institute for Cancer Research
> PO Box 2008
> Royal Melbourne Hospital
> Victoria, 3050
> AUSTRALIA
>
> Office: [phone]
> Mobile: [phone]
> Fax: [phone]
>
> Facility Website<http://www.ludwig.edu.au/confocal/>
>
>
>
>
>
>
>
> This communication is intended only for the named recipient and may contain information that is confidential, legally privileged or subject to copyright; the Ludwig Institute for Cancer Research Ltd does not waive any rights if you have received this communication in error.
> The views expressed in this communication are those of the sender and do not necessarily reflect the views of the Ludwig Institute for Cancer Research Ltd.
>
>    

--


George McNamara, PhD
Analytical Imaging Core Facility
University of Miami