Open space construcction to Microscopy Facility

[Jorge]

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Dear Community
I want to request the help of this fabulous community in the construction
of our new facility that will gather at least seven equipment in a single
workspace (Light Sheet, Seahorse, 2 Confocal, 2 epifluorescences, 1
Cellomics).
I have the motivation that this space is a unique open space (something
similar to an emergency room of a hospital). Each microscope separated with
good quality curtains and with well-directed light on the equipment.
Moreover, when no equipment is used to have a beautiful view of all the
equipment at the same time.
Based on this motivate, I have several doubts that I hope you can help me.
We will greatly appreciate it since we are making an enormous investment
for us and we have no references for this in this regard.

   - Have any of you considered or built space of this style ?, (several
   microscopes in a single open space only separated with curtains), could you
   share your experience and ideally a photo?
   - Do you know any curtain and curtain rails ideal for this type of
   construction?
   - Concerning lighting, do you have some type of lamps or bulbs to focus
   the light over the microscope only?.
   - do you know some photochromatic glass or similar material that allows
   shown microscopes, but that blocks it completely when we are working and
   prevents light from entering from outside? (with a microscopy facility
   quality).


Thanks for all
greetings

--
Jorge Toledo H
Ph.D.
Biomedical Neuroscience Institute (BNI)
Laboratory of Scientific Image Analysis (SCIAN-Lab)
Faculty of Medicine
University of Chile
www.bni.cl | www.scian.cl

[Zdenek Svindrych-2]

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I remember seeing something similar in MPI-CBG, a huge room with many
machines in it, separated by curtains.
But, each room also had regular door for entry, think of it as a big room
inside a building, so that the room has a number of doors from each side.
One microscope behind each door. The individual workspaces are still
divided by heavy curtains, but you don't normally have to move them at all.
It's more problematic when users need to pass through the curtains, as they
tend to be heavy and cumbersome. This results in the curtains being open
all the time.
But that's OK, too. You don't really need a dark room for Seahorse, not
even for a confocal. It's more critical with epifluorescence scopes and
TIRFs, and paramount in case of multiphoton imaging. With lightsheet ...
well ... it depends, Z.1 can sit on your office desk, but Phaseview alpha
would definitely benefit from a dark room, preferentially vacuum and
suspended in zero gravity.
 - curtains and rails: haven't found the right ones yet. With rails made of
short segments the curtains tend to get stuck at the segment boundaries, so
the skill of the person mounting them is important.
 - lights: dimmable fake fluorescent ceiling lights (LEDs) are OK (assuming
your curtains go all the away to the top), it's great if users can control
the light while sitting by the scope.
 - what glass? Never seen that. I would avoid glass in dark rooms.
Absolutely.
Good luck with your new facility!
$0.02
zdenek


On Fri, Aug 23, 2019 at 12:21 PM Jorge <[hidden email]> wrote:

> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> Post images on http://www.imgur.com and include the link in your posting.
> *****
>
> Dear Community
> I want to request the help of this fabulous community in the construction
> of our new facility that will gather at least seven equipment in a single
> workspace (Light Sheet, Seahorse, 2 Confocal, 2 epifluorescences, 1
> Cellomics).
> I have the motivation that this space is a unique open space (something
> similar to an emergency room of a hospital). Each microscope separated with
> good quality curtains and with well-directed light on the equipment.
> Moreover, when no equipment is used to have a beautiful view of all the
> equipment at the same time.
> Based on this motivate, I have several doubts that I hope you can help me.
> We will greatly appreciate it since we are making an enormous investment
> for us and we have no references for this in this regard.
>
>    - Have any of you considered or built space of this style ?, (several
>    microscopes in a single open space only separated with curtains), could
> you
>    share your experience and ideally a photo?
>    - Do you know any curtain and curtain rails ideal for this type of
>    construction?
>    - Concerning lighting, do you have some type of lamps or bulbs to focus
>    the light over the microscope only?.
>    - do you know some photochromatic glass or similar material that allows
>    shown microscopes, but that blocks it completely when we are working and
>    prevents light from entering from outside? (with a microscopy facility
>    quality).
>
>
> Thanks for all
> greetings
>
> --
> Jorge Toledo H
> Ph.D.
> Biomedical Neuroscience Institute (BNI)
> Laboratory of Scientific Image Analysis (SCIAN-Lab)
> Faculty of Medicine
> University of Chile
> www.bni.cl | www.scian.cl
>

--
--
Zdenek Svindrych, Ph.D.
Research Associate - Imaging Specialist
Department of Biochemistry and Cell Biology
Geisel School of Medicine at Dartmouth

[Craig Brideau]

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*****

If your curtains go all the way up to the ceiling, you will need to
consider the heat getting trapped in each pocket and need separate air
supply to each curtained area. If the curtains do not go up to the ceiling,
the heat should mix within the room, simplifying overall climate control
needs. The drawback is limiting light into the curtained areas when they
don't' go to the ceiling. You might want a hybrid approach, where you have
some curtains placed lower, and one or two areas up to the ceiling with
extra ventilation. In this case, a system like a multiphoton, which
requires darkness *and* generates a substantial heat load, will be
satisfied.

Craig

On Fri, Aug 23, 2019 at 11:26 AM Zdenek Svindrych <[hidden email]> wrote:

> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> Post images on http://www.imgur.com and include the link in your posting.
> *****
>
> I remember seeing something similar in MPI-CBG, a huge room with many
> machines in it, separated by curtains.
> But, each room also had regular door for entry, think of it as a big room
> inside a building, so that the room has a number of doors from each side.
> One microscope behind each door. The individual workspaces are still
> divided by heavy curtains, but you don't normally have to move them at all.
> It's more problematic when users need to pass through the curtains, as they
> tend to be heavy and cumbersome. This results in the curtains being open
> all the time.
> But that's OK, too. You don't really need a dark room for Seahorse, not
> even for a confocal. It's more critical with epifluorescence scopes and
> TIRFs, and paramount in case of multiphoton imaging. With lightsheet ...
> well ... it depends, Z.1 can sit on your office desk, but Phaseview alpha
> would definitely benefit from a dark room, preferentially vacuum and
> suspended in zero gravity.
>  - curtains and rails: haven't found the right ones yet. With rails made of
> short segments the curtains tend to get stuck at the segment boundaries, so
> the skill of the person mounting them is important.
>  - lights: dimmable fake fluorescent ceiling lights (LEDs) are OK (assuming
> your curtains go all the away to the top), it's great if users can control
> the light while sitting by the scope.
>  - what glass? Never seen that. I would avoid glass in dark rooms.
> Absolutely.
> Good luck with your new facility!
> $0.02
> zdenek
>
>
> On Fri, Aug 23, 2019 at 12:21 PM Jorge <[hidden email]> wrote:
>
> > *****
> > To join, leave or search the confocal microscopy listserv, go to:
> > http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> > Post images on http://www.imgur.com and include the link in your
> posting.
> > *****
> >
> > Dear Community
> > I want to request the help of this fabulous community in the construction
> > of our new facility that will gather at least seven equipment in a single
> > workspace (Light Sheet, Seahorse, 2 Confocal, 2 epifluorescences, 1
> > Cellomics).
> > I have the motivation that this space is a unique open space (something
> > similar to an emergency room of a hospital). Each microscope separated
> with
> > good quality curtains and with well-directed light on the equipment.
> > Moreover, when no equipment is used to have a beautiful view of all the
> > equipment at the same time.
> > Based on this motivate, I have several doubts that I hope you can help
> me.
> > We will greatly appreciate it since we are making an enormous investment
> > for us and we have no references for this in this regard.
> >
> >    - Have any of you considered or built space of this style ?, (several
> >    microscopes in a single open space only separated with curtains),
> could
> > you
> >    share your experience and ideally a photo?
> >    - Do you know any curtain and curtain rails ideal for this type of
> >    construction?
> >    - Concerning lighting, do you have some type of lamps or bulbs to
> focus
> >    the light over the microscope only?.
> >    - do you know some photochromatic glass or similar material that
> allows
> >    shown microscopes, but that blocks it completely when we are working
> and
> >    prevents light from entering from outside? (with a microscopy facility
> >    quality).
> >
> >
> > Thanks for all
> > greetings
> >
> > --
> > Jorge Toledo H
> > Ph.D.
> > Biomedical Neuroscience Institute (BNI)
> > Laboratory of Scientific Image Analysis (SCIAN-Lab)
> > Faculty of Medicine
> > University of Chile
> > www.bni.cl | www.scian.cl
> >
>
>
> --
> --
> Zdenek Svindrych, Ph.D.
> Research Associate - Imaging Specialist
> Department of Biochemistry and Cell Biology
> Geisel School of Medicine at Dartmouth
>

[Douglas Richardson]

*****
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*****

Our facility is completely open concept. I love it and would never go back
to individual rooms.

The facility is always quite dark (I have the bruises to prove it) as each
microscope is illuminated by its own remote controlled spot lights that are
usually off except when someone is loading/unloading a sample.  Each scope
is separated by cheap, potable office/cubical dividers.  The dividers are
easy to move around when we exchange equipment and they block what little
stray light there may be from a neighboring system's spot lights.

The one exception is our multi-photon platform.  It is housed in a corner
of the facility behind a floor to ceiling black curtain. Additional
ventilation exists within this space, although after a day of constant
imaging it is often a few degrees warmer than the rest of the room (all
ventilation is HEPA filtered and low flow).

The open concept encourages collaboration and leads to all kinds of "wow,
what is that!?" types of discussions. I can honestly say, it's brought a
number of labs together.

-Doug

On Fri, Aug 23, 2019 at 1:55 PM Craig Brideau <[hidden email]>
wrote:

> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> Post images on http://www.imgur.com and include the link in your posting.
> *****
>
> If your curtains go all the way up to the ceiling, you will need to
> consider the heat getting trapped in each pocket and need separate air
> supply to each curtained area. If the curtains do not go up to the ceiling,
> the heat should mix within the room, simplifying overall climate control
> needs. The drawback is limiting light into the curtained areas when they
> don't' go to the ceiling. You might want a hybrid approach, where you have
> some curtains placed lower, and one or two areas up to the ceiling with
> extra ventilation. In this case, a system like a multiphoton, which
> requires darkness *and* generates a substantial heat load, will be
> satisfied.
>
> Craig
>
> On Fri, Aug 23, 2019 at 11:26 AM Zdenek Svindrych <[hidden email]>
> wrote:
>
> > *****
> > To join, leave or search the confocal microscopy listserv, go to:
> > http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> > Post images on http://www.imgur.com and include the link in your
> posting.
> > *****
> >
> > I remember seeing something similar in MPI-CBG, a huge room with many
> > machines in it, separated by curtains.
> > But, each room also had regular door for entry, think of it as a big room
> > inside a building, so that the room has a number of doors from each side.
> > One microscope behind each door. The individual workspaces are still
> > divided by heavy curtains, but you don't normally have to move them at
> all.
> > It's more problematic when users need to pass through the curtains, as
> they
> > tend to be heavy and cumbersome. This results in the curtains being open
> > all the time.
> > But that's OK, too. You don't really need a dark room for Seahorse, not
> > even for a confocal. It's more critical with epifluorescence scopes and
> > TIRFs, and paramount in case of multiphoton imaging. With lightsheet ...
> > well ... it depends, Z.1 can sit on your office desk, but Phaseview alpha
> > would definitely benefit from a dark room, preferentially vacuum and
> > suspended in zero gravity.
> >  - curtains and rails: haven't found the right ones yet. With rails made
> of
> > short segments the curtains tend to get stuck at the segment boundaries,
> so
> > the skill of the person mounting them is important.
> >  - lights: dimmable fake fluorescent ceiling lights (LEDs) are OK
> (assuming
> > your curtains go all the away to the top), it's great if users can
> control
> > the light while sitting by the scope.
> >  - what glass? Never seen that. I would avoid glass in dark rooms.
> > Absolutely.
> > Good luck with your new facility!
> > $0.02
> > zdenek
> >
> >
> > On Fri, Aug 23, 2019 at 12:21 PM Jorge <[hidden email]> wrote:
> >
> > > *****
> > > To join, leave or search the confocal microscopy listserv, go to:
> > > http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> > > Post images on http://www.imgur.com and include the link in your
> > posting.
> > > *****
> > >
> > > Dear Community
> > > I want to request the help of this fabulous community in the
> construction
> > > of our new facility that will gather at least seven equipment in a
> single
> > > workspace (Light Sheet, Seahorse, 2 Confocal, 2 epifluorescences, 1
> > > Cellomics).
> > > I have the motivation that this space is a unique open space (something
> > > similar to an emergency room of a hospital). Each microscope separated
> > with
> > > good quality curtains and with well-directed light on the equipment.
> > > Moreover, when no equipment is used to have a beautiful view of all the
> > > equipment at the same time.
> > > Based on this motivate, I have several doubts that I hope you can help
> > me.
> > > We will greatly appreciate it since we are making an enormous
> investment
> > > for us and we have no references for this in this regard.
> > >
> > >    - Have any of you considered or built space of this style ?,
> (several
> > >    microscopes in a single open space only separated with curtains),
> > could
> > > you
> > >    share your experience and ideally a photo?
> > >    - Do you know any curtain and curtain rails ideal for this type of
> > >    construction?
> > >    - Concerning lighting, do you have some type of lamps or bulbs to
> > focus
> > >    the light over the microscope only?.
> > >    - do you know some photochromatic glass or similar material that
> > allows
> > >    shown microscopes, but that blocks it completely when we are working
> > and
> > >    prevents light from entering from outside? (with a microscopy
> facility
> > >    quality).
> > >
> > >
> > > Thanks for all
> > > greetings
> > >
> > > --
> > > Jorge Toledo H
> > > Ph.D.
> > > Biomedical Neuroscience Institute (BNI)
> > > Laboratory of Scientific Image Analysis (SCIAN-Lab)
> > > Faculty of Medicine
> > > University of Chile
> > > www.bni.cl | www.scian.cl
> > >
> >
> >
> > --
> > --
> > Zdenek Svindrych, Ph.D.
> > Research Associate - Imaging Specialist
> > Department of Biochemistry and Cell Biology
> > Geisel School of Medicine at Dartmouth
> >
>

[leavesley]

*****
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http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
Post images on http://www.imgur.com and include the link in your posting.
*****

We had curtains in the lab I was in for grad school for a while. They
were black-out curtains with an additional valence that hung from the
rail to provide overlap and to minimize light.  They were just OK. 
Sometimes they were cumbersome, but we worked around it.  One thing to
note - the shed.  Especially the ones that get moved back and forth.  If
you have any specific instruments that suck in large amounts of air with
poor dust-handling capabilities, then the optics in those systems could
get dirty quickly.  I guess you could maybe try for some other type of
curtain material, maybe vinyl like really thick black shower curtains,
if such a thing exists.  Good luck and best wishes for a successful design!

Best regards,

Silas


On 8/23/2019 12:50 PM, Craig Brideau wrote:

> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> Post images on http://www.imgur.com and include the link in your posting.
> *****
>
> If your curtains go all the way up to the ceiling, you will need to
> consider the heat getting trapped in each pocket and need separate air
> supply to each curtained area. If the curtains do not go up to the ceiling,
> the heat should mix within the room, simplifying overall climate control
> needs. The drawback is limiting light into the curtained areas when they
> don't' go to the ceiling. You might want a hybrid approach, where you have
> some curtains placed lower, and one or two areas up to the ceiling with
> extra ventilation. In this case, a system like a multiphoton, which
> requires darkness *and* generates a substantial heat load, will be
> satisfied.
>
> Craig
>
> On Fri, Aug 23, 2019 at 11:26 AM Zdenek Svindrych <[hidden email]> wrote:
>
>> *****
>> To join, leave or search the confocal microscopy listserv, go to:
>> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
>> Post images on http://www.imgur.com and include the link in your posting.
>> *****
>>
>> I remember seeing something similar in MPI-CBG, a huge room with many
>> machines in it, separated by curtains.
>> But, each room also had regular door for entry, think of it as a big room
>> inside a building, so that the room has a number of doors from each side.
>> One microscope behind each door. The individual workspaces are still
>> divided by heavy curtains, but you don't normally have to move them at all.
>> It's more problematic when users need to pass through the curtains, as they
>> tend to be heavy and cumbersome. This results in the curtains being open
>> all the time.
>> But that's OK, too. You don't really need a dark room for Seahorse, not
>> even for a confocal. It's more critical with epifluorescence scopes and
>> TIRFs, and paramount in case of multiphoton imaging. With lightsheet ...
>> well ... it depends, Z.1 can sit on your office desk, but Phaseview alpha
>> would definitely benefit from a dark room, preferentially vacuum and
>> suspended in zero gravity.
>>   - curtains and rails: haven't found the right ones yet. With rails made of
>> short segments the curtains tend to get stuck at the segment boundaries, so
>> the skill of the person mounting them is important.
>>   - lights: dimmable fake fluorescent ceiling lights (LEDs) are OK (assuming
>> your curtains go all the away to the top), it's great if users can control
>> the light while sitting by the scope.
>>   - what glass? Never seen that. I would avoid glass in dark rooms.
>> Absolutely.
>> Good luck with your new facility!
>> $0.02
>> zdenek
>>
>>
>> On Fri, Aug 23, 2019 at 12:21 PM Jorge <[hidden email]> wrote:
>>
>>> *****
>>> To join, leave or search the confocal microscopy listserv, go to:
>>> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
>>> Post images on http://www.imgur.com and include the link in your
>> posting.
>>> *****
>>>
>>> Dear Community
>>> I want to request the help of this fabulous community in the construction
>>> of our new facility that will gather at least seven equipment in a single
>>> workspace (Light Sheet, Seahorse, 2 Confocal, 2 epifluorescences, 1
>>> Cellomics).
>>> I have the motivation that this space is a unique open space (something
>>> similar to an emergency room of a hospital). Each microscope separated
>> with
>>> good quality curtains and with well-directed light on the equipment.
>>> Moreover, when no equipment is used to have a beautiful view of all the
>>> equipment at the same time.
>>> Based on this motivate, I have several doubts that I hope you can help
>> me.
>>> We will greatly appreciate it since we are making an enormous investment
>>> for us and we have no references for this in this regard.
>>>
>>>     - Have any of you considered or built space of this style ?, (several
>>>     microscopes in a single open space only separated with curtains),
>> could
>>> you
>>>     share your experience and ideally a photo?
>>>     - Do you know any curtain and curtain rails ideal for this type of
>>>     construction?
>>>     - Concerning lighting, do you have some type of lamps or bulbs to
>> focus
>>>     the light over the microscope only?.
>>>     - do you know some photochromatic glass or similar material that
>> allows
>>>     shown microscopes, but that blocks it completely when we are working
>> and
>>>     prevents light from entering from outside? (with a microscopy facility
>>>     quality).
>>>
>>>
>>> Thanks for all
>>> greetings
>>>
>>> --
>>> Jorge Toledo H
>>> Ph.D.
>>> Biomedical Neuroscience Institute (BNI)
>>> Laboratory of Scientific Image Analysis (SCIAN-Lab)
>>> Faculty of Medicine
>>> University of Chile
>>> www.bni.cl | www.scian.cl
>>>
>>
>> --
>> --
>> Zdenek Svindrych, Ph.D.
>> Research Associate - Imaging Specialist
>> Department of Biochemistry and Cell Biology
>> Geisel School of Medicine at Dartmouth
>>

--
Silas J. Leavesley, Ph.D.
Professor
Department of Chemical and Biomolecular Engineering
Department of Pharmacology
Center for Lung Biology
University of South Alabama
150 Jaguar Drive, SH4129
Mobile, AL 36688
ph: (251)-460-6160
fax: [phone]
web: http://www.southalabama.edu/centers/bioimaging
google scholar: http://scholar.google.co.uk/citations?user=knkwcj4AAAAJ

[Christopher Yip]

*****
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*****

Not sure how this frames up but our biosafety folks are not keen on any fabric materials in our imaging spaces if in Level 2 labs / spaces  - has to be hard surfaces or washable. So fabric blackouts are a no-go...

Christopher Yip PhD, P.Eng,
-
 

On 2019-08-23, 3:09 PM, "Confocal Microscopy List on behalf of Silas Leavesley" <[hidden email] on behalf of [hidden email]> wrote:

    *****
    To join, leave or search the confocal microscopy listserv, go to:
    http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
    Post images on http://www.imgur.com and include the link in your posting.
    *****
   
    We had curtains in the lab I was in for grad school for a while. They
    were black-out curtains with an additional valence that hung from the
    rail to provide overlap and to minimize light.  They were just OK.  
    Sometimes they were cumbersome, but we worked around it.  One thing to
    note - the shed.  Especially the ones that get moved back and forth.  If
    you have any specific instruments that suck in large amounts of air with
    poor dust-handling capabilities, then the optics in those systems could
    get dirty quickly.  I guess you could maybe try for some other type of
    curtain material, maybe vinyl like really thick black shower curtains,
    if such a thing exists.  Good luck and best wishes for a successful design!
   
    Best regards,
   
    Silas
   
   
    On 8/23/2019 12:50 PM, Craig Brideau wrote:
    > *****
    > To join, leave or search the confocal microscopy listserv, go to:
    > http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
    > Post images on http://www.imgur.com and include the link in your posting.
    > *****
    >
    > If your curtains go all the way up to the ceiling, you will need to
    > consider the heat getting trapped in each pocket and need separate air
    > supply to each curtained area. If the curtains do not go up to the ceiling,
    > the heat should mix within the room, simplifying overall climate control
    > needs. The drawback is limiting light into the curtained areas when they
    > don't' go to the ceiling. You might want a hybrid approach, where you have
    > some curtains placed lower, and one or two areas up to the ceiling with
    > extra ventilation. In this case, a system like a multiphoton, which
    > requires darkness *and* generates a substantial heat load, will be
    > satisfied.
    >
    > Craig
    >
    > On Fri, Aug 23, 2019 at 11:26 AM Zdenek Svindrych <[hidden email]> wrote:
    >
    >> *****
    >> To join, leave or search the confocal microscopy listserv, go to:
    >> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
    >> Post images on http://www.imgur.com and include the link in your posting.
    >> *****
    >>
    >> I remember seeing something similar in MPI-CBG, a huge room with many
    >> machines in it, separated by curtains.
    >> But, each room also had regular door for entry, think of it as a big room
    >> inside a building, so that the room has a number of doors from each side.
    >> One microscope behind each door. The individual workspaces are still
    >> divided by heavy curtains, but you don't normally have to move them at all.
    >> It's more problematic when users need to pass through the curtains, as they
    >> tend to be heavy and cumbersome. This results in the curtains being open
    >> all the time.
    >> But that's OK, too. You don't really need a dark room for Seahorse, not
    >> even for a confocal. It's more critical with epifluorescence scopes and
    >> TIRFs, and paramount in case of multiphoton imaging. With lightsheet ...
    >> well ... it depends, Z.1 can sit on your office desk, but Phaseview alpha
    >> would definitely benefit from a dark room, preferentially vacuum and
    >> suspended in zero gravity.
    >>   - curtains and rails: haven't found the right ones yet. With rails made of
    >> short segments the curtains tend to get stuck at the segment boundaries, so
    >> the skill of the person mounting them is important.
    >>   - lights: dimmable fake fluorescent ceiling lights (LEDs) are OK (assuming
    >> your curtains go all the away to the top), it's great if users can control
    >> the light while sitting by the scope.
    >>   - what glass? Never seen that. I would avoid glass in dark rooms.
    >> Absolutely.
    >> Good luck with your new facility!
    >> $0.02
    >> zdenek
    >>
    >>
    >> On Fri, Aug 23, 2019 at 12:21 PM Jorge <[hidden email]> wrote:
    >>
    >>> *****
    >>> To join, leave or search the confocal microscopy listserv, go to:
    >>> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
    >>> Post images on http://www.imgur.com and include the link in your
    >> posting.
    >>> *****
    >>>
    >>> Dear Community
    >>> I want to request the help of this fabulous community in the construction
    >>> of our new facility that will gather at least seven equipment in a single
    >>> workspace (Light Sheet, Seahorse, 2 Confocal, 2 epifluorescences, 1
    >>> Cellomics).
    >>> I have the motivation that this space is a unique open space (something
    >>> similar to an emergency room of a hospital). Each microscope separated
    >> with
    >>> good quality curtains and with well-directed light on the equipment.
    >>> Moreover, when no equipment is used to have a beautiful view of all the
    >>> equipment at the same time.
    >>> Based on this motivate, I have several doubts that I hope you can help
    >> me.
    >>> We will greatly appreciate it since we are making an enormous investment
    >>> for us and we have no references for this in this regard.
    >>>
    >>>     - Have any of you considered or built space of this style ?, (several
    >>>     microscopes in a single open space only separated with curtains),
    >> could
    >>> you
    >>>     share your experience and ideally a photo?
    >>>     - Do you know any curtain and curtain rails ideal for this type of
    >>>     construction?
    >>>     - Concerning lighting, do you have some type of lamps or bulbs to
    >> focus
    >>>     the light over the microscope only?.
    >>>     - do you know some photochromatic glass or similar material that
    >> allows
    >>>     shown microscopes, but that blocks it completely when we are working
    >> and
    >>>     prevents light from entering from outside? (with a microscopy facility
    >>>     quality).
    >>>
    >>>
    >>> Thanks for all
    >>> greetings
    >>>
    >>> --
    >>> Jorge Toledo H
    >>> Ph.D.
    >>> Biomedical Neuroscience Institute (BNI)
    >>> Laboratory of Scientific Image Analysis (SCIAN-Lab)
    >>> Faculty of Medicine
    >>> University of Chile
    >>> www.bni.cl | www.scian.cl
    >>>
    >>
    >> --
    >> --
    >> Zdenek Svindrych, Ph.D.
    >> Research Associate - Imaging Specialist
    >> Department of Biochemistry and Cell Biology
    >> Geisel School of Medicine at Dartmouth
    >>
    --
    Silas J. Leavesley, Ph.D.
    Professor
    Department of Chemical and Biomolecular Engineering
    Department of Pharmacology
    Center for Lung Biology
    University of South Alabama
    150 Jaguar Drive, SH4129
    Mobile, AL 36688
    ph: (251)-460-6160
    fax: [phone]
    web: http://www.southalabama.edu/centers/bioimaging
    google scholar: http://scholar.google.co.uk/citations?user=knkwcj4AAAAJ
   

[leavesley]

*****
To join, leave or search the confocal microscopy listserv, go to:
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
Post images on http://www.imgur.com and include the link in your posting.
*****

It's the same here.  They made us paint a wood frame that had a faraday
cage with lacquer just so it could be scrubbed down.

Best regards,

Silas


On 8/23/2019 2:17 PM, Christopher Yip wrote:

> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> Post images on http://www.imgur.com and include the link in your posting.
> *****
>
> Not sure how this frames up but our biosafety folks are not keen on any fabric materials in our imaging spaces if in Level 2 labs / spaces  - has to be hard surfaces or washable. So fabric blackouts are a no-go...
>
> Christopher Yip PhD, P.Eng,
> -
>  
>
> On 2019-08-23, 3:09 PM, "Confocal Microscopy List on behalf of Silas Leavesley" <[hidden email] on behalf of [hidden email]> wrote:
>
>      *****
>      To join, leave or search the confocal microscopy listserv, go to:
>      http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
>      Post images on http://www.imgur.com and include the link in your posting.
>      *****
>      
>      We had curtains in the lab I was in for grad school for a while. They
>      were black-out curtains with an additional valence that hung from the
>      rail to provide overlap and to minimize light.  They were just OK.
>      Sometimes they were cumbersome, but we worked around it.  One thing to
>      note - the shed.  Especially the ones that get moved back and forth.  If
>      you have any specific instruments that suck in large amounts of air with
>      poor dust-handling capabilities, then the optics in those systems could
>      get dirty quickly.  I guess you could maybe try for some other type of
>      curtain material, maybe vinyl like really thick black shower curtains,
>      if such a thing exists.  Good luck and best wishes for a successful design!
>      
>      Best regards,
>      
>      Silas
>      
>      
>      On 8/23/2019 12:50 PM, Craig Brideau wrote:
>      > *****
>      > To join, leave or search the confocal microscopy listserv, go to:
>      > http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
>      > Post images on http://www.imgur.com and include the link in your posting.
>      > *****
>      >
>      > If your curtains go all the way up to the ceiling, you will need to
>      > consider the heat getting trapped in each pocket and need separate air
>      > supply to each curtained area. If the curtains do not go up to the ceiling,
>      > the heat should mix within the room, simplifying overall climate control
>      > needs. The drawback is limiting light into the curtained areas when they
>      > don't' go to the ceiling. You might want a hybrid approach, where you have
>      > some curtains placed lower, and one or two areas up to the ceiling with
>      > extra ventilation. In this case, a system like a multiphoton, which
>      > requires darkness *and* generates a substantial heat load, will be
>      > satisfied.
>      >
>      > Craig
>      >
>      > On Fri, Aug 23, 2019 at 11:26 AM Zdenek Svindrych <[hidden email]> wrote:
>      >
>      >> *****
>      >> To join, leave or search the confocal microscopy listserv, go to:
>      >> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
>      >> Post images on http://www.imgur.com and include the link in your posting.
>      >> *****
>      >>
>      >> I remember seeing something similar in MPI-CBG, a huge room with many
>      >> machines in it, separated by curtains.
>      >> But, each room also had regular door for entry, think of it as a big room
>      >> inside a building, so that the room has a number of doors from each side.
>      >> One microscope behind each door. The individual workspaces are still
>      >> divided by heavy curtains, but you don't normally have to move them at all.
>      >> It's more problematic when users need to pass through the curtains, as they
>      >> tend to be heavy and cumbersome. This results in the curtains being open
>      >> all the time.
>      >> But that's OK, too. You don't really need a dark room for Seahorse, not
>      >> even for a confocal. It's more critical with epifluorescence scopes and
>      >> TIRFs, and paramount in case of multiphoton imaging. With lightsheet ...
>      >> well ... it depends, Z.1 can sit on your office desk, but Phaseview alpha
>      >> would definitely benefit from a dark room, preferentially vacuum and
>      >> suspended in zero gravity.
>      >>   - curtains and rails: haven't found the right ones yet. With rails made of
>      >> short segments the curtains tend to get stuck at the segment boundaries, so
>      >> the skill of the person mounting them is important.
>      >>   - lights: dimmable fake fluorescent ceiling lights (LEDs) are OK (assuming
>      >> your curtains go all the away to the top), it's great if users can control
>      >> the light while sitting by the scope.
>      >>   - what glass? Never seen that. I would avoid glass in dark rooms.
>      >> Absolutely.
>      >> Good luck with your new facility!
>      >> $0.02
>      >> zdenek
>      >>
>      >>
>      >> On Fri, Aug 23, 2019 at 12:21 PM Jorge <[hidden email]> wrote:
>      >>
>      >>> *****
>      >>> To join, leave or search the confocal microscopy listserv, go to:
>      >>> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
>      >>> Post images on http://www.imgur.com and include the link in your
>      >> posting.
>      >>> *****
>      >>>
>      >>> Dear Community
>      >>> I want to request the help of this fabulous community in the construction
>      >>> of our new facility that will gather at least seven equipment in a single
>      >>> workspace (Light Sheet, Seahorse, 2 Confocal, 2 epifluorescences, 1
>      >>> Cellomics).
>      >>> I have the motivation that this space is a unique open space (something
>      >>> similar to an emergency room of a hospital). Each microscope separated
>      >> with
>      >>> good quality curtains and with well-directed light on the equipment.
>      >>> Moreover, when no equipment is used to have a beautiful view of all the
>      >>> equipment at the same time.
>      >>> Based on this motivate, I have several doubts that I hope you can help
>      >> me.
>      >>> We will greatly appreciate it since we are making an enormous investment
>      >>> for us and we have no references for this in this regard.
>      >>>
>      >>>     - Have any of you considered or built space of this style ?, (several
>      >>>     microscopes in a single open space only separated with curtains),
>      >> could
>      >>> you
>      >>>     share your experience and ideally a photo?
>      >>>     - Do you know any curtain and curtain rails ideal for this type of
>      >>>     construction?
>      >>>     - Concerning lighting, do you have some type of lamps or bulbs to
>      >> focus
>      >>>     the light over the microscope only?.
>      >>>     - do you know some photochromatic glass or similar material that
>      >> allows
>      >>>     shown microscopes, but that blocks it completely when we are working
>      >> and
>      >>>     prevents light from entering from outside? (with a microscopy facility
>      >>>     quality).
>      >>>
>      >>>
>      >>> Thanks for all
>      >>> greetings
>      >>>
>      >>> --
>      >>> Jorge Toledo H
>      >>> Ph.D.
>      >>> Biomedical Neuroscience Institute (BNI)
>      >>> Laboratory of Scientific Image Analysis (SCIAN-Lab)
>      >>> Faculty of Medicine
>      >>> University of Chile
>      >>> www.bni.cl | www.scian.cl
>      >>>
>      >>
>      >> --
>      >> --
>      >> Zdenek Svindrych, Ph.D.
>      >> Research Associate - Imaging Specialist
>      >> Department of Biochemistry and Cell Biology
>      >> Geisel School of Medicine at Dartmouth
>      >>
>      --
>      Silas J. Leavesley, Ph.D.
>      Professor
>      Department of Chemical and Biomolecular Engineering
>      Department of Pharmacology
>      Center for Lung Biology
>      University of South Alabama
>      150 Jaguar Drive, SH4129
>      Mobile, AL 36688
>      ph: (251)-460-6160
>      fax: [phone]
>      web: http://www.southalabama.edu/centers/bioimaging
>      google scholar: http://scholar.google.co.uk/citations?user=knkwcj4AAAAJ
>      
>

--
Silas J. Leavesley, Ph.D.
Professor
Department of Chemical and Biomolecular Engineering
Department of Pharmacology
Center for Lung Biology
University of South Alabama
150 Jaguar Drive, SH4129
Mobile, AL 36688
ph: (251)-460-6160
fax: [phone]
web: http://www.southalabama.edu/centers/bioimaging
google scholar: http://scholar.google.co.uk/citations?user=knkwcj4AAAAJ

[Christopher Yip]

*****
To join, leave or search the confocal microscopy listserv, go to:
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
Post images on http://www.imgur.com and include the link in your posting.
*****

No cardboard boxes allowed in our level 2 spaces either....

Christopher Yip PhD, P.Eng,

On 2019-08-23, 4:07 PM, "Confocal Microscopy List on behalf of Silas Leavesley" <[hidden email] on behalf of [hidden email]> wrote:

    *****
    To join, leave or search the confocal microscopy listserv, go to:
    http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
    Post images on http://www.imgur.com and include the link in your posting.
    *****
   
    It's the same here.  They made us paint a wood frame that had a faraday
    cage with lacquer just so it could be scrubbed down.
   
    Best regards,
   
    Silas
   
   
    On 8/23/2019 2:17 PM, Christopher Yip wrote:
    > *****
    > To join, leave or search the confocal microscopy listserv, go to:
    > http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
    > Post images on http://www.imgur.com and include the link in your posting.
    > *****
    >
    > Not sure how this frames up but our biosafety folks are not keen on any fabric materials in our imaging spaces if in Level 2 labs / spaces  - has to be hard surfaces or washable. So fabric blackouts are a no-go...
    >
    > Christopher Yip PhD, P.Eng,
    > -
    >  
    >
    > On 2019-08-23, 3:09 PM, "Confocal Microscopy List on behalf of Silas Leavesley" <[hidden email] on behalf of [hidden email]> wrote:
    >
    >      *****
    >      To join, leave or search the confocal microscopy listserv, go to:
    >      http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
    >      Post images on http://www.imgur.com and include the link in your posting.
    >      *****
    >      
    >      We had curtains in the lab I was in for grad school for a while. They
    >      were black-out curtains with an additional valence that hung from the
    >      rail to provide overlap and to minimize light.  They were just OK.
    >      Sometimes they were cumbersome, but we worked around it.  One thing to
    >      note - the shed.  Especially the ones that get moved back and forth.  If
    >      you have any specific instruments that suck in large amounts of air with
    >      poor dust-handling capabilities, then the optics in those systems could
    >      get dirty quickly.  I guess you could maybe try for some other type of
    >      curtain material, maybe vinyl like really thick black shower curtains,
    >      if such a thing exists.  Good luck and best wishes for a successful design!
    >      
    >      Best regards,
    >      
    >      Silas
    >      
    >      
    >      On 8/23/2019 12:50 PM, Craig Brideau wrote:
    >      > *****
    >      > To join, leave or search the confocal microscopy listserv, go to:
    >      > http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
    >      > Post images on http://www.imgur.com and include the link in your posting.
    >      > *****
    >      >
    >      > If your curtains go all the way up to the ceiling, you will need to
    >      > consider the heat getting trapped in each pocket and need separate air
    >      > supply to each curtained area. If the curtains do not go up to the ceiling,
    >      > the heat should mix within the room, simplifying overall climate control
    >      > needs. The drawback is limiting light into the curtained areas when they
    >      > don't' go to the ceiling. You might want a hybrid approach, where you have
    >      > some curtains placed lower, and one or two areas up to the ceiling with
    >      > extra ventilation. In this case, a system like a multiphoton, which
    >      > requires darkness *and* generates a substantial heat load, will be
    >      > satisfied.
    >      >
    >      > Craig
    >      >
    >      > On Fri, Aug 23, 2019 at 11:26 AM Zdenek Svindrych <[hidden email]> wrote:
    >      >
    >      >> *****
    >      >> To join, leave or search the confocal microscopy listserv, go to:
    >      >> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
    >      >> Post images on http://www.imgur.com and include the link in your posting.
    >      >> *****
    >      >>
    >      >> I remember seeing something similar in MPI-CBG, a huge room with many
    >      >> machines in it, separated by curtains.
    >      >> But, each room also had regular door for entry, think of it as a big room
    >      >> inside a building, so that the room has a number of doors from each side.
    >      >> One microscope behind each door. The individual workspaces are still
    >      >> divided by heavy curtains, but you don't normally have to move them at all.
    >      >> It's more problematic when users need to pass through the curtains, as they
    >      >> tend to be heavy and cumbersome. This results in the curtains being open
    >      >> all the time.
    >      >> But that's OK, too. You don't really need a dark room for Seahorse, not
    >      >> even for a confocal. It's more critical with epifluorescence scopes and
    >      >> TIRFs, and paramount in case of multiphoton imaging. With lightsheet ...
    >      >> well ... it depends, Z.1 can sit on your office desk, but Phaseview alpha
    >      >> would definitely benefit from a dark room, preferentially vacuum and
    >      >> suspended in zero gravity.
    >      >>   - curtains and rails: haven't found the right ones yet. With rails made of
    >      >> short segments the curtains tend to get stuck at the segment boundaries, so
    >      >> the skill of the person mounting them is important.
    >      >>   - lights: dimmable fake fluorescent ceiling lights (LEDs) are OK (assuming
    >      >> your curtains go all the away to the top), it's great if users can control
    >      >> the light while sitting by the scope.
    >      >>   - what glass? Never seen that. I would avoid glass in dark rooms.
    >      >> Absolutely.
    >      >> Good luck with your new facility!
    >      >> $0.02
    >      >> zdenek
    >      >>
    >      >>
    >      >> On Fri, Aug 23, 2019 at 12:21 PM Jorge <[hidden email]> wrote:
    >      >>
    >      >>> *****
    >      >>> To join, leave or search the confocal microscopy listserv, go to:
    >      >>> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
    >      >>> Post images on http://www.imgur.com and include the link in your
    >      >> posting.
    >      >>> *****
    >      >>>
    >      >>> Dear Community
    >      >>> I want to request the help of this fabulous community in the construction
    >      >>> of our new facility that will gather at least seven equipment in a single
    >      >>> workspace (Light Sheet, Seahorse, 2 Confocal, 2 epifluorescences, 1
    >      >>> Cellomics).
    >      >>> I have the motivation that this space is a unique open space (something
    >      >>> similar to an emergency room of a hospital). Each microscope separated
    >      >> with
    >      >>> good quality curtains and with well-directed light on the equipment.
    >      >>> Moreover, when no equipment is used to have a beautiful view of all the
    >      >>> equipment at the same time.
    >      >>> Based on this motivate, I have several doubts that I hope you can help
    >      >> me.
    >      >>> We will greatly appreciate it since we are making an enormous investment
    >      >>> for us and we have no references for this in this regard.
    >      >>>
    >      >>>     - Have any of you considered or built space of this style ?, (several
    >      >>>     microscopes in a single open space only separated with curtains),
    >      >> could
    >      >>> you
    >      >>>     share your experience and ideally a photo?
    >      >>>     - Do you know any curtain and curtain rails ideal for this type of
    >      >>>     construction?
    >      >>>     - Concerning lighting, do you have some type of lamps or bulbs to
    >      >> focus
    >      >>>     the light over the microscope only?.
    >      >>>     - do you know some photochromatic glass or similar material that
    >      >> allows
    >      >>>     shown microscopes, but that blocks it completely when we are working
    >      >> and
    >      >>>     prevents light from entering from outside? (with a microscopy facility
    >      >>>     quality).
    >      >>>
    >      >>>
    >      >>> Thanks for all
    >      >>> greetings
    >      >>>
    >      >>> --
    >      >>> Jorge Toledo H
    >      >>> Ph.D.
    >      >>> Biomedical Neuroscience Institute (BNI)
    >      >>> Laboratory of Scientific Image Analysis (SCIAN-Lab)
    >      >>> Faculty of Medicine
    >      >>> University of Chile
    >      >>> www.bni.cl | www.scian.cl
    >      >>>
    >      >>
    >      >> --
    >      >> --
    >      >> Zdenek Svindrych, Ph.D.
    >      >> Research Associate - Imaging Specialist
    >      >> Department of Biochemistry and Cell Biology
    >      >> Geisel School of Medicine at Dartmouth
    >      >>
    >      --
    >      Silas J. Leavesley, Ph.D.
    >      Professor
    >      Department of Chemical and Biomolecular Engineering
    >      Department of Pharmacology
    >      Center for Lung Biology
    >      University of South Alabama
    >      150 Jaguar Drive, SH4129
    >      Mobile, AL 36688
    >      ph: (251)-460-6160
    >      fax: [phone]
    >      web: http://www.southalabama.edu/centers/bioimaging
    >      google scholar: http://scholar.google.co.uk/citations?user=knkwcj4AAAAJ
    >      
    >
    --
    Silas J. Leavesley, Ph.D.
    Professor
    Department of Chemical and Biomolecular Engineering
    Department of Pharmacology
    Center for Lung Biology
    University of South Alabama
    150 Jaguar Drive, SH4129
    Mobile, AL 36688
    ph: (251)-460-6160
    fax: [phone]
    web: http://www.southalabama.edu/centers/bioimaging
    google scholar: http://scholar.google.co.uk/citations?user=knkwcj4AAAAJ