Opening for Senior AFM Scientist at the Memorial Sloan-Kettering Cancer Center

[Vitaly Boyko-2]

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 Dear All,
we have an exciting opportunity at the Molecular Cytology Core Facility of the Memorial Sloan-Kettering Cancer Center. Please find it below:

Atomic Force Microscopist, PhD Senior Scientist – Molecular Cytology Core

Sloan Kettering Institute, Memorial Sloan Kettering Cancer Center

 

The Molecular Cytology Core facility at Sloan Kettering Institute is seeking a senior research scientist to lead the planning and the execution of Atomic Force Microscopy (AFM) experiments with the members of the research and clinical laboratories at MSKCC and neighbor Institutes.

The Atomic Force Microscopist is expected to work as a member of the Core team, focusing specifically on the AFM, to continue the expansion of its use and assist in the development of new methods for the scientists. While his/her role would entail a great deal of AFM work, s/he is required to understand and work with the optical systems to facilitate and catalyze projects and cross-collaboration.

 

The ideal candidate should have:

·         PhD degree in Biological Science with advanced AFM experience and strong computational background

·         Excellent communication skills and ability to work in a team environment

·         Strong organization skills, flexibility in working hours and ability to multitask

·         Scientific maturity, ability to understand the project goals and to assure the generation of the state-of-the-art AFM data

 

Please send CV, a letter outlining your interest and names/contact information of three references via email to:

 

Yevgeniy Romin, Molecular Cytology Core Facility, Sloan Kettering Institute

[hidden email]

 

Rachel Kong, Molecular Cytology Core Facility, Sloan Kettering Institute

[hidden email]

 
 

 

    On Wednesday, September 11, 2019, 02:35:44 PM EDT, Vitaly Boyko <[hidden email]> wrote:  
 
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 Hi Romain,
thank you very much for the useful information.In simpler terms, we plan to use ididi or labtek II 4-well dishes with 170 um bottom, with four inlets and four outlets, and add fixed amount of a drug/dye, test different drugs at different concentrations, etc. in the range of 100-300 ul, remove or replace the media, be able to control (calibrate) the flow rate, volume, etc.Most of perfusion systems handle single well dishes (e.g. delta T dish with inserts which is not practical along basic drug assays), with one inlet/one outlet, based on a gravity flowNikon NIS may work via serial (it functions via TTL pulses).
How much effort would it be to integrate it into the Nikon NIS and have it running smoothly?
Best,
Vitaly



    On Wednesday, September 11, 2019, 03:13:31 AM EDT, Romain Laine <[hidden email]> wrote: 
 
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Dear Vitaly,
Depending on the types of experiments that you have in mind and your
budget, you may want to consider using our NanoJ-Fluidics system.
https://www.nature.com/articles/s41467-019-09231-9
It's an open-source, cheap perfusion system for on-microscope treatments of
sample.

It's really easy to multiplex and you can control volumes and rates but
there's some limitations in terms of the complexity of simultaneous
perfusion.
I have not tried interfacing it with NIS but there may be a way via serial.
It's also not a big commitment to build a couple and give it a shot.

I hope this helps !

Romain

On Tue, Sep 10, 2019 at 9:35 PM Vitaly Boyko <
[hidden email]> wrote:

> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> Post images on http://www.imgur.com and include the link in your posting.
> *****
>
>  Dear All,
> We are looking for an advanced perfusion system with up to 8 pumps that
> can be programmed to monitor input and output media volume, flow rate, and
> more, and compatible with the Nikon NES software.Any recommendations will
> be greatly appreciated.
> Best regards,
> VitalyMSKCC/MCCF
>
>
>
>