Optical cement mounting media

[Rickman, Colin]

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Hi

There was an email exchange about fluorescent spheres mounted on slides in an optical cement. I think it was about the prepared Molecular Probes slides. I've tried to search for the email exchange but can't find it. I'm trying to locate the supplier of the optical cement used which had an RI of around 1.5.

Does anybody remember the emails and the supplier?

Thanks

Colin

Dr Colin Rickman
Life Science Interface
Department of Chemistry (WP 2.03)
School of Engineering and Physical Sciences
Heriot-Watt University
Edinburgh
EH14 4AS

Tel: [phone] (Office)

http://www.lifescienceinterface.hw.ac.uk
http://www.eps.hw.ac.uk/departments/chemistry/cr.htm



--
Heriot-Watt University is a Scottish charity
registered under charity number SC000278.

[Shivaprasad Bhuvanendran-2]

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Hi,

I do not remember the email exchange but from what I learned at the Optical
Microscopy course at Woods Hole, Norland Optical Cement # 63 supplied by
Edmund Scientific should work. The RI for # 63 is 1.56.

Interestingly, I learned it from another Colin though it was Dr. Colin
Izzard!

Best regards,
Shiva

PS: No commercial interest.

Shivaprasad Bhuvanendran
Research Support Specialist - Bio-Imaging
The Rockefeller University
1230 York Avenue
Box 209 (DWB 201)
New York NY 10065
tel [phone]
fax [phone]




On Fri, May 13, 2011 at 9:43 AM, Colin Rickman <[hidden email]> wrote:

> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> *****
>
> Hi
>
> There was an email exchange about fluorescent spheres mounted on slides in
> an optical cement. I think it was about the prepared Molecular Probes
> slides. I've tried to search for the email exchange but can't find it. I'm
> trying to locate the supplier of the optical cement used which had an RI of
> around 1.5.
>
> Does anybody remember the emails and the supplier?
>
> Thanks
>
> Colin
>
> Dr Colin Rickman
> Life Science Interface
> Department of Chemistry (WP 2.03)
> School of Engineering and Physical Sciences
> Heriot-Watt University
> Edinburgh
> EH14 4AS
>
> Tel: [phone] (Office)
>
> http://www.lifescienceinterface.hw.ac.uk
> http://www.eps.hw.ac.uk/departments/chemistry/cr.htm
>
>
>
> --
> Heriot-Watt University is a Scottish charity
> registered under charity number SC000278.
>

[John Oreopoulos]

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I think you're referring to an email I posted about a conversation I had with Iain Johnson at Molecular Probes about the TetraSpeck Beads (no commercial interest). I've copied it here again for you:


***********************
John:

The mounting medium is Saint Gobain Optical Cement.  According to my notes, this has a refractive index of 1.52 when cured.

The spectra for the TetraSpeck green dye are the same as our standard yellow/green FluoSpheres:http://www.invitrogen.com/site/us/en/home/support/Product-Technical-Resources/Product-Spectra.8811h2o.html

The spectra for the Tetraspeck dark red  dye are the same as our standard dark red FluoSpheres:http://www.invitrogen.com/site/us/en/home/support/Product-Technical-Resources/Product-Spectra.8807h2o.html

The blue and orange dyes are different from the standard FluoSpheres dyes so I don't have data for those at present.  I'll work on getting it but may take a few weeks.

Best Regards,

iain
***********************

Cheers

John Oreopoulos
Research Assistant
Spectral Applied Research
Richmond Hill, Ontario
Canada
www.spectral.ca


On 2011-05-13, at 10:10 AM, Shivaprasad Bhuvanendran wrote:

> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> *****
>
> Hi,
>
> I do not remember the email exchange but from what I learned at the Optical
> Microscopy course at Woods Hole, Norland Optical Cement # 63 supplied by
> Edmund Scientific should work. The RI for # 63 is 1.56.
>
> Interestingly, I learned it from another Colin though it was Dr. Colin
> Izzard!
>
> Best regards,
> Shiva
>
> PS: No commercial interest.
>
> Shivaprasad Bhuvanendran
> Research Support Specialist - Bio-Imaging
> The Rockefeller University
> 1230 York Avenue
> Box 209 (DWB 201)
> New York NY 10065
> tel [phone]
> fax [phone]
>
>
>
>
> On Fri, May 13, 2011 at 9:43 AM, Colin Rickman <[hidden email]> wrote:
>
>> *****
>> To join, leave or search the confocal microscopy listserv, go to:
>> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
>> *****
>>
>> Hi
>>
>> There was an email exchange about fluorescent spheres mounted on slides in
>> an optical cement. I think it was about the prepared Molecular Probes
>> slides. I've tried to search for the email exchange but can't find it. I'm
>> trying to locate the supplier of the optical cement used which had an RI of
>> around 1.5.
>>
>> Does anybody remember the emails and the supplier?
>>
>> Thanks
>>
>> Colin
>>
>> Dr Colin Rickman
>> Life Science Interface
>> Department of Chemistry (WP 2.03)
>> School of Engineering and Physical Sciences
>> Heriot-Watt University
>> Edinburgh
>> EH14 4AS
>>
>> Tel: [phone] (Office)
>>
>> http://www.lifescienceinterface.hw.ac.uk
>> http://www.eps.hw.ac.uk/departments/chemistry/cr.htm
>>
>>
>>
>> --
>> Heriot-Watt University is a Scottish charity
>> registered under charity number SC000278.
>>