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Hi Eric, we have good results with 860nm for dual green/red, and would suggest GFP and mCherry.
Cheers,
Brian D Armstrong PhD
Assistant Research Professor
Director, Light Microscopy Core
Beckman Research Institute
City of Hope
Dept of Neuroscience
1450 E Duarte Rd
Duarte, CA 91010
626-256-4673 x62872
http://www.cityofhope.org/research/support/Light-Microscopy-Digital-Imaging/Pages/default.aspx-----Original Message-----
From: Confocal Microscopy List [mailto:
[hidden email]] On Behalf Of Eric Olson
Sent: Friday, April 13, 2012 8:22 AM
To:
[hidden email]
Subject: Optimal two-photon dual-color imaging using eGFP and . . . .?
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Dear List,
We are imaging cortical neurons in a transgenic mouse embryo (eGFP expressing). We can express a second FP by electroporation and want to visualize both FPs at one excitation wavelength.
Any recommendations for another FP partner for eGFP? Possibly DsRed? And what excitation wavelength is used to excite both FPs? Our Chameleon laser tunes 700 to 950nm, but without much energy at the longer wavelengths.
Thanks,
Eric
Eric C. Olson, Ph.D.
Department of Neuroscience and Physiology
SUNY Upstate Medical University
3295 Weiskotten Hall
766 Irving St.
Syracuse, NY 13210
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