All,
Thanks to all for the suggestions. I think we will end up trying
some combination of these helpful hints.
We are trying to image GFPuv expressing rickettsia in living ticks
that have been partially dissected, and explants from living ticks.
The rickettsia are motile within the tick cells. We have done this
routinely in cultured cells, but this invivo imaging is (or course)
more challenging for a number of reasons. If anyone has additional
comment, please keep them coming!
Mike
Michael J. Herron, U of MN, Dept. of Entomology
[hidden email]
612-624-3688 (office) 612-625-5299 (FAX)
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