PSF fitting in widefield microscopy

[Sergey Tauger]

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Dear all,

Could you please recommend a free software (and a manual for it) to perform the
following task?

I have an image of microtubules. For each microtubule, I drew a ROI around
corresponding microtubule, 7 pixels wide, and decomposed it into series of 1x7
pixel "images" of microtubule cross-section. I would like to fit PSF for given
optical condition to each of cross-section and obtain the values: goodness-of-
fit, estimated maximum value, and estimated maximum position.

Yes, it is like STORM, but I don't know if software presented in
Zhuang Research Lab homepage suits the task.

Best,
Sergey

[Neil Kad]

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Try this Gaussian fitter, if I understand correctly what you are trying to achieve this will work straight from ImageJ.

http://kadlab.demojoomla.com/index.php/links-and-downloads/9-downloads

Choose Gaussian Fitter





 



Cheers,

 

Neil

 

=================================

Neil M. Kad PhD.,

School of Biosciences,

University of Kent,

Canterbury,

Kent CT2 7NH,

United Kingdom.

 

Tel: [phone]

=================================

> Date: Tue, 21 Oct 2014 11:53:52 -0500
> From: [hidden email]
> Subject: PSF fitting in widefield microscopy
> To: [hidden email]
>
> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> Post images on http://www.imgur.com and include the link in your posting.
> *****
>
> Dear all,
>
> Could you please recommend a free software (and a manual for it) to perform the
> following task?
>
> I have an image of microtubules. For each microtubule, I drew a ROI around
> corresponding microtubule, 7 pixels wide, and decomposed it into series of 1x7
> pixel "images" of microtubule cross-section. I would like to fit PSF for given
> optical condition to each of cross-section and obtain the values: goodness-of-
> fit, estimated maximum value, and estimated maximum position.
>
> Yes, it is like STORM, but I don't know if software presented in
> Zhuang Research Lab homepage suits the task.
>
> Best,
> Sergey