Confocal Microscopy List

Part 2: Image acquisition software query

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Martin Spitaler

Part 2: Image acquisition software query

Dear Vitaly,

in our facility we use both Volocity from Improvision (now owned by Perkin Elmer) and SimplePCI (now renamed to HCImage) from C-Imaging (now owned by Hamamatsu). Volocity does the job, but SimplePCI is very popular with our users for its intuitive handling and flexibility, and it has many more features like on the fly shade correction, background subtraction and ratiometric measurements, filter chromatic abberration correction etc. It's Hamamatsu though, so won't support every camera.

Martin
PS: no commercial interest

Martin Spitaler, PhD

FILM - Facility for Imaging by Light Microscopy
- Facility Manager -
Sir Alexander Fleming Building, desk 401
Imperial College London / South Kensington
Exhibition Road
London SW7 2AZ
UK

Tel. +44-(0)20-759-42023
E-mail [hidden email]
Website: http://imperial.ac.uk/imagingfacility

`Subject:`	`Part 2: Image acquisition software query`
`From:`	`Vitaly Boyko [hidden email]`
`Reply-To:`	`[hidden email]`
`Date:`	`Mon, 29 Jun 2009 12:41:51 -0500`
`Content-Type:`	`text/plain`

Dear List,

Another simple question on the acquisition.

Which image acquisition software (if not, then ... Let's be patient ...
Image Analysis software) is the most-user friendly, broad and flexible in
terms of image histogram analysis and display, with an exceptionally
"attractive" GUI interface!, including user-defined, multi-ROI background
subtraction - or highly accurate automatic background detection/subtraction,
manual and automatic thresholding, automatic means, stdev, st_error,
histogram export options and so on ... 

I do not mention at this point 'subimage, subpixel image registration', but
it could be exciting to learn if there are some recent additions to image
analysis software(s) that include the latter option.

A little hint - zero point is OpenLab (primitive histograms, lengthy, time
consuming manual work), next, slightly better option is Volocity but a kind
of user-unfriendly and flexibility remains in "a sweet dream territory". 

Very much thankful in advance,

Vitaly

NCI-Frederick,
301-846-6575

Casey Laris

Application Scientist for High-Content Screening (HCS), Image Cytometry and Automated Cell Image Analysis Company in San Diego

Vala Sciences Inc.

Application Scientist - High-Content Screening (HCS), Image Cytometry and Automated Cell Image Analysis

Vala Sciences Inc is a fast-growing San Diego-based biotechnology company that offers cell based assay reagents and automated cell image analysis software to academic, pharmaceutical and biotechnology scientists. Vala Sciences' assay kits and CyteSeer Automated Cell Image Analysis Software can be applied to images acquired from any high-content screening or digital microscope system. Vala's intellectual property position and key leading research collaborations all drive towards creating information-rich high throughput measurements for making fundamentally new insights into cell function.

Vala Sciences Application Scientist will be responsible for:

· Undertaking research projects and studies to expand the range of applications for Vala Sciences' products

· Performing feasibility studies and preparing reports for potential customers

· Developing protocols and experimental procedures for customers

· Collaborating with others on the preparation of Vala Sciences’ technical materials, seminars, scientific meetings, and publications

· Consulting with customers to devise new applications, protocols and approaches for use on Vala Sciences' products

· Management of all customer feasibility studies and for-fee projects

· Serving as a "communication bridge" between end-users and internal development groups.

The successful candidate will have:

· An advanced degree in the biological sciences, with a minimum of five years experience in confocal microscopy, high-content screening, flow cytometry, cellular imaging, or a related biomedical field.

· Thorough knowledge of appropriate scientific methods and techniques, with extensive experience in cellular immunology, high-content cellular analysis, or molecular biology.

· The ability to speak with authority with peers inside and outside the Company on relevant scientific matters

· Excellent interpersonal, communication and presentation skills, both oral and written.

· Previous experience working within and towards the goals of a commercially driven organization.

· Previous experience working with HCS vendors (i.e. Thermo Fisher Cellomics ArrayScan, Perkin Elmer/Evotec Opera, Becton Dickinson (BD/Atto Pathway), Beckman Coulter/Q3DM (IC 100), General Electric (GE InCell 1000), Applied Precision DeltaVision)

Location & Travel:

· The location of this position is flexible with priority given to candidates in San Diego, Houston, San Francisco, Boston, Research Triangle Park.

· Candidates should expect 20% travel for this position.

We are looking for a hands-on professional who has the excellent scientific depth and the interpersonal and communication skills needed for success in a small company. This person must be self-directed with considerable initiative. It is important that the person demonstrate the ability to learn complicated information quickly.

Vala Sciences is an equal opportunity employer and offers a fully competitive salary and benefits package.

Vala offers a superb working environment for the self-starting, hard working creative professional. Most positions allow for flexible work schedules in an open collaborative environment with many opportunities for new roles and responsibilities.

Candidates can apply with a CV and salary history to [hidden email].

Principals only. Recruiters, please don't reply to this posting.