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There are several particle tracking plugins for ImageJ.
I like the one from Mosaic group at ETH
http://www.mosaic.ethz.ch/Downloads/ParticleTracker - documentation is
given there as well.
This plugin has a nice interface that gives important feedback on how
user adjustable parameters influence the results: identifying and
positioning particles in each image and then linking positions in
subsequent frames into trajectories. With this plugin can see
identified particles in images and resulting trajectories laid over
stacks and adjust parameters to gain confidence that it is working with
your data.
Depending on what you want to do, it is the analysis of the trajectories
that is the interesting part. I don't know about anything in ImageJ
that does this.
Some trajectory analysis are integrated in the Matlab particle tracking
code from Maria Kilfoil:
http://people.umass.edu/kilfoil/downloads.html - detailed instructions
are there, but not too much on the meaning of the analysis.
This code as well as other particle tracking software implementations
are derived from IDL code from Crocker Grier and Weeks.
http://www.physics.emory.edu/~weeks/idl/index.html This page is a bit
out of date.
A reference which is both a good "how-to" on particle-tracking and an
introduction to its use for microrheology:
Crocker and Hoffmann "Multiple‐Particle Tracking and Two‐Point
Microrheology in Cells" Methods in Cell Biology 83:141-178
http://www.sciencedirect.com/science/article/pii/S0091679X0783007XOn Thu, 2012-02-02 at 13:15 -0500, yuansheng sun wrote:
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> To join, leave or search the confocal microscopy listserv, go to:
>
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy> *****
>
> Dear Listers,
>
> I am looking for a free ImageJ plugin for particle tracking. Any
> recommendation would be highly appreciated. Thanks in advance.
>
> Best regards,
> Sheng
--
Andrew Ridsdale
Steacie Institute for Molecular Sciences
National Research Council of Canada
100 Sussex Drive
Ottawa ON K1A 0R6
Canada
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