Many thanks to all of you who offered advice on pelleting cells after ethanol fixation. That's what they did in the end, and it worked:
"Here is the protocol I used for pelleting EtOH-fixed cells:
1. Pellet cells in EtOH fix at 750g, 4 min.
2. Wash cell pellet with 1X PBS supplemented with 1% FBS or BSA.
3. Allow to equilibrate for 20 minutes at room temperature while rocking.
4. Centrifuge cells in a swing bucket centrifuge at 1800g for 4
minutes. *Note* A fixed-radius centrifuge does not pellet the cells at
this step even at high g forces."
Michael Model, Ph.D.
Confocal Microscopy,
Dpt Biological Sciences,
Kent State University, Kent, OH 44023
tel. 330-672-2874
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