Phase contrast microscopy

[Arvydas Matiukas]

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Dear Core Managers/Directors:
 
Please share how you manage users/configurations and track usage
on confocal systems. Specifically I am interested in
systems using ZEN software which does not have
built-in multiuser/tracking capabilities.

We just moved to new building so there is good reason to
improve management of two confocal systems running ZEN
(one is new and one upgraded from AIM).
I already installed following features:
1. online calendar to book time
2.  card access to the confocal rooms
3.  Greyware logon monitor to record username and logon/logoff time.
 
I consider creating windows account for each lab. This will
allow to monitor usage using Greyware. Please advise if there
are other simple and efficient ways to track usage as well as
set different confocal configurations for differing users.
 
Finally, to fight dust/dirt we consider requiring to change shoes
before entering the confocal rooms, and placing sticky/static
mats outside (to trap dust from footwear). Maybe somebody is
using such mats and can advise on the brand/model.
 
Thanks,
Arvydas
 
 
 
Arvydas Matiukas, Ph.D.
Director of Confocal&Two-Photon Core
SUNY Upstate Medical University
Neuroscience & Physiology Dept

Room 4607 NRB
505 Irving Ave
Syracuse, NY 13210
Tel.: [phone]
Fax: [phone]
Email: [hidden email]

[Cromey, Douglas W - (dcromey)]

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Regarding the sticky mats, you could ask the histology lab in a nearby hospital.  Many use them to keep the paraffin wax from being tracked out of the lab and creating a hallway slipping hazard.

Doug

^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^
Douglas W. Cromey, M.S. - Associate Scientific Investigator
Dept. of Cellular & Molecular Medicine, University of Arizona
1501 N. Campbell Ave, Tucson, AZ  85724-5044 USA

office:  AHSC 4212         email: [hidden email]
voice:  520-626-2824       fax:  [phone]

http://swehsc.pharmacy.arizona.edu/micro
Home of: "Microscopy and Imaging Resources on the WWW"

UA Microscopy Alliance -  http://microscopy.arizona.edu


-----Original Message-----
From: Confocal Microscopy List [mailto:[hidden email]] On Behalf Of Arvydas Matiukas
Sent: Thursday, March 27, 2014 3:16 PM
To: [hidden email]
Subject: Re: Managing multiple users

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To join, leave or search the confocal microscopy listserv, go to:
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
Post images on http://www.imgur.com and include the link in your posting.
*****

Dear Core Managers/Directors:
 
Please share how you manage users/configurations and track usage on confocal systems. Specifically I am interested in systems using ZEN software which does not have built-in multiuser/tracking capabilities.

We just moved to new building so there is good reason to improve management of two confocal systems running ZEN (one is new and one upgraded from AIM).
I already installed following features:
1. online calendar to book time
2.  card access to the confocal rooms
3.  Greyware logon monitor to record username and logon/logoff time.
 
I consider creating windows account for each lab. This will allow to monitor usage using Greyware. Please advise if there are other simple and efficient ways to track usage as well as set different confocal configurations for differing users.
 
Finally, to fight dust/dirt we consider requiring to change shoes before entering the confocal rooms, and placing sticky/static mats outside (to trap dust from footwear). Maybe somebody is using such mats and can advise on the brand/model.
 
Thanks,
Arvydas
 
 
 
Arvydas Matiukas, Ph.D.
Director of Confocal&Two-Photon Core
SUNY Upstate Medical University
Neuroscience & Physiology Dept

Room 4607 NRB
505 Irving Ave
Syracuse, NY 13210
Tel.: [phone]
Fax: [phone]
Email: [hidden email]

[Craig Brideau]

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I use the 3M Clean Walk mat:

http://www.thorlabs.com/newgrouppage9.cfm?objectgroup_id=2862

http://www.grainger.com/product/3M-Clean-Walk-Mat-2GJJ4

It helps somewhat, but you have to remember to peel off fresh sheets fairly
frequently.

Craig Brideau


On Thu, Mar 27, 2014 at 4:19 PM, Cromey, Douglas W - (dcromey) <
[hidden email]> wrote:

> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> Post images on http://www.imgur.com and include the link in your posting.
> *****
>
> Regarding the sticky mats, you could ask the histology lab in a nearby
> hospital.  Many use them to keep the paraffin wax from being tracked out of
> the lab and creating a hallway slipping hazard.
>
> Doug
>
> ^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^
> Douglas W. Cromey, M.S. - Associate Scientific Investigator
> Dept. of Cellular & Molecular Medicine, University of Arizona
> 1501 N. Campbell Ave, Tucson, AZ  85724-5044 USA
>
> office:  AHSC 4212         email: [hidden email]
> voice:  520-626-2824       fax:  [phone]
>
> http://swehsc.pharmacy.arizona.edu/micro
> Home of: "Microscopy and Imaging Resources on the WWW"
>
> UA Microscopy Alliance -  http://microscopy.arizona.edu
>
>
> -----Original Message-----
> From: Confocal Microscopy List [mailto:[hidden email]]
> On Behalf Of Arvydas Matiukas
> Sent: Thursday, March 27, 2014 3:16 PM
> To: [hidden email]
> Subject: Re: Managing multiple users
>
> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> Post images on http://www.imgur.com and include the link in your posting.
> *****
>
> Dear Core Managers/Directors:
>
> Please share how you manage users/configurations and track usage on
> confocal systems. Specifically I am interested in systems using ZEN
> software which does not have built-in multiuser/tracking capabilities.
>
> We just moved to new building so there is good reason to improve
> management of two confocal systems running ZEN (one is new and one upgraded
> from AIM).
> I already installed following features:
> 1. online calendar to book time
> 2.  card access to the confocal rooms
> 3.  Greyware logon monitor to record username and logon/logoff time.
>
> I consider creating windows account for each lab. This will allow to
> monitor usage using Greyware. Please advise if there are other simple and
> efficient ways to track usage as well as set different confocal
> configurations for differing users.
>
> Finally, to fight dust/dirt we consider requiring to change shoes before
> entering the confocal rooms, and placing sticky/static mats outside (to
> trap dust from footwear). Maybe somebody is using such mats and can advise
> on the brand/model.
>
> Thanks,
> Arvydas
>
>
>
> Arvydas Matiukas, Ph.D.
> Director of Confocal&Two-Photon Core
> SUNY Upstate Medical University
> Neuroscience & Physiology Dept
>
> Room 4607 NRB
> 505 Irving Ave
> Syracuse, NY 13210
> Tel.: [phone]
> Fax: [phone]
> Email: [hidden email]
>

[Sergey Tauger]

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Michael,

S.S.Kou's transport-of-intensity approach seems to be 2D. Is it somehow
possible to map refractive indexes in 3D without interpolation between 2D
data for different optical slices?
And one more question: could you please recommend an open-source
software for optical path difference calculation regardless of the optical
setup used (DIC, brightfield, phase contrast...)? I cannot get the
underlying math, the code usually makes it more easy to understand.

Thanks in advance,
Sergey

[mmodel]

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Sorry Sergey, don't have the answer. Maybe Shalin could help? You can also contact Laura Waller at Berkeley, she is very knowledgeable in these questions. And if you are in Russia, you might try talking to Vladimir Tychinsky or Alexander Tikhonov in Moscow

-----Original Message-----
From: Confocal Microscopy List [mailto:[hidden email]] On Behalf Of Sergey Tauger
Sent: Friday, March 28, 2014 9:00 AM
To: [hidden email]
Subject: Re: Phase contrast microscopy

*****
To join, leave or search the confocal microscopy listserv, go to:
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
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*****

Michael,

S.S.Kou's transport-of-intensity approach seems to be 2D. Is it somehow possible to map refractive indexes in 3D without interpolation between 2D data for different optical slices?
And one more question: could you please recommend an open-source software for optical path difference calculation regardless of the optical setup used (DIC, brightfield, phase contrast...)? I cannot get the underlying math, the code usually makes it more easy to understand.

Thanks in advance,
Sergey